Effects of Crataegi Fructus Hot-Water Extract on Hair Growth and Growth Factor Gene Expression of Human Dermal Papilla Cells

산사 열수추출물의 모발 성장과 모유두세포의 성장인자 유전자 발현에 대한 영향

  • Kim, Hyeong-Key (Department of Meridian & Acupoint, College of Korean Medicine, Dong-Eui University) ;
  • Kim, Cheol-Hong (Department of Acupuncture & Moxibustion, College of Korean Medicine, Dong-Eui University) ;
  • Yoon, Hyun-Min (Department of Acupuncture & Moxibustion, College of Korean Medicine, Dong-Eui University) ;
  • Kang, Kyung-Hwa (Department of Physiology, College of Korean Medicine, Dong-Eui University) ;
  • Song, Choon-Ho (Department of Meridian & Acupoint, College of Korean Medicine, Dong-Eui University)
  • 김형기 (동의대학교 한의과대학 경락경혈학교실) ;
  • 김철홍 (동의대학교 한의과대학 침구학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구학교실) ;
  • 강경화 (동의대학교 한의과대학 생리학교실) ;
  • 송춘호 (동의대학교 한의과대학 경락경혈학교실)
  • Received : 2017.08.29
  • Accepted : 2017.09.22
  • Published : 2017.09.27


Objectives : This study was carried out to investigate the effects of Crataegi Fructus water extract(CFWE) on hair growth in an alopecia model of C57BL/6N mice and human dermal papilla cells(hDPCs). Methods : Six-week old mice were depilated and separated in 3 groups ; CON, MXD(2% Minoxidil), and CFWE. The treatments were applied twice a day for 18 days. The hair growth was determined photographically. The hair density, thickness and length were identified by Folliscope and the weights of body were measured. In dorsal skin tissue, the expression of hair growth-related protein was analyzed by Western blot. In hDPCs with/without $IFN-{\gamma}$, cell proliferation and the expression of hair growth-related genes were analyzed. Results : We observed that CFWE promoted hair growth compared to CON. CFWE improved the hair density, thickness and length compared to CON. CFWE increased the $Wnt/{\beta}$-catenin signaling in dorsal skin. In hDPCs, CFWE accelerated the cell proliferation and inhibited $IFN-{\gamma}$-induced hDPCs degeneration. CFWE increased the mRNA expression of ${\beta}$-catenin, Axin-2, BMP-4, FGF-7, FGF-10, and ALP compared to CON and $IFN-{\gamma}$ treated cells. Conclusions : These results suggest that CFWE has a hair regrowth activity via $Wnt/{\beta}$-catenin signaling and can be useful for the treatment of alopecia.



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