The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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Effects of Ganoderma Lucidum Extract Ethanol Extract and Microneedle Therapy System on Hair Growth in an Alopecia Model of C57BL/6N Mice

C57BL/6N 마우스 탈모모델에서 미세다륜침과 영지추출물이 모발성장에 미치는 영향

  • Ju, Bong Hyun (Dept. of Pediatrics, College of Oriental Medicine, Dongeui University) ;
  • Yu, Sun Ae (Dept. of Pediatrics, College of Oriental Medicine, Dongeui University) ;
  • Kang, Kyung Hwa (Dept. of Oriental Physiology, College of Oriental Medicine, Dongeui University) ;
  • Lee, Seung Yoen (Dept. of Pediatrics, College of Oriental Medicine, Dongeui University)
  • 주봉현 (동의대학교 한의과대학 소아과교실) ;
  • 유선애 (동의대학교 한의과대학 소아과교실) ;
  • 강경화 (동의대학교 한의과대학 생리학교실) ;
  • 이승연 (동의대학교 한의과대학 소아과교실)
  • Received : 2014.05.03
  • Accepted : 2014.05.20
  • Published : 2014.05.31
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Abstract

Objectives This study was carried out to investigate the effect of Ganoderma lucidum extract (GLE) and microneedle therapy system (MTS) on hair growth in an alopecia model of C57BL/6N mice. Methods Five-week old mice were depilated and separated in 4 groups ; CON (50% EtOH), MXD (5% Minoxidil), MTS and GLE + MTS. The treatments were applied twice a week for 3 weeks. The hair growth was determined photographically, the hair density, thickness and length were identified by Folliscope and the weights of body and organs were measured. In dorsal skin tissue, the expression of hair growth-related gene and protein was analyzed by RT - PCR or Western blot. In addition, the hair follicles in the dermis were observed by H&E staining. Results The promotion of hair growth was observed in GLE + MTS and MTS compared to CON. The hair density, thickness and length were also improved in GLE + MTS and MTS compared to CON. The mRNA expression of TGF-${\alpha}$, TGF-${\beta}1$, IGF-1, PRL and PL and the protein expression of VEGF and IGF-1 were increased in GLE + MTS and MTS compared to CON. The hair follicles and hair root growth were improved in GLE + MTS and MTS compared to CON. In the above results, GLE + MTS were more effective than MTS. Conclusions These results suggest that GLE and MTS has a hair growth activity and can be useful for the treatment of alopecia.

Keywords

References

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