• Title/Summary/Keyword: laser alignment

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The Effect of S130A Mutant of pharaonis Halorhodopsin on Ability of Chloride Binding and Photocycle

  • Sato, Maki;Kikukawa, Takashi;Araiso, Tsunehisa;Okita, Hirotaka;Shimono, Kazumi;Kamo, Naoki;Demura, Makoto;Nitta, Katsutoshi
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.308-310
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    • 2002
  • Bacteriorhodopsin (bR) and halorhodopsin (hR), which exist in the membrane of Halobacterium salinarum, are light-driven ion pumps. In spite of high similarity of primary and tertiary structures between bR and hR, these membrane proteins transport different ions, proton and chloride, in the opposite direction. From alignment of the amino acid sequences, Thr-89 of bR is homologous to Ser-l15 of hR from Halobacterium salinarum (shR). X-ray structure of shR has revealed that OH group of this residue directly interacts with CI$\^$-/ Thus, Ser-lI5 of shR is expected to play an important role in CI$\^$-/ binding and transport. In this study, we expressed wild type hR from Natronobacterium pharaonis (PhR) and Sl30A, which corresponds to Ser-l15 of shR, in E. coli in order to clarify binding affinity of chloride ion and photocycle reactions. From the titration with CI$\^$-/, affinity of Sl30A became quite lower than that of WT (WT 6 mM, Sl30A 89 mM). Furthermore, from the flash photolysis with pulse laser of λ$\_$max/ at 532 nm, the reaction rate of SI30A from 0 intermediate to hR ground state was found to become apparently slower than that of WT. The singular value decomposition (SVD) and global fitting analyses of the photocycles were performed to identify all photointermediates and determine the reaction rates.

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Growth of vertically aligned carbon nanotubes on Co-Ni alloy metal (Co-Ni 합금위에서 수직방향으로 정렬된 탄소나노튜브의 성장)

  • Lee, Cheol-Jin;Kim, Dae-Woon;Lee, Tae-Jae;Park, Jeong-Hoon;Son, Kwon-Hee;Lyu, Seung-Chul;Song, Hong-Ki;Choi, Young-Chul;Lee, Young-Hee
    • Proceedings of the KIEE Conference
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    • 1999.07d
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    • pp.1504-1507
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    • 1999
  • We have grown vertically aligned carbon nanotubes in a large area of Co-Ni codeposited Si substrates by the thermal CVD using $C_2H_2$ gas. Since the discovery of carbon nanotubes, Synthesis of carbon nanotubes for mass production has been achieved by several methods such as laser vaporization arc discharge, and pyrolysis. In particular, growth of vertically aligned nanotubes is of technological importance for applications to FED. Recently, vertically aligned carbon nanotubes have been grown on glass by PECVD Aligned carbon nanotubes can be also grown on mesoporous silica and Fe patterned porous silicon using CVD. Despite such breakthroughs in the growth, the growth mechanism of the alignment are still far from being clearly understood. Furthermore, FED has not been clearly demonstrated yet at a practical level. Here, we demonstrate that carbon nanotubes can be vertically aligned on catalyzed Si substrate when the domain density reaches a certain value. We suggest that steric hindrance between nanotubes at an initial stage of the growth forces nanotubes to align vertically and then nanotubes are further grown by the cap growth mechanism.

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Measurements of Turbulent How in $5\times{5}$ PWR Rod Bundles With Spacer Grids (지지격자를 갖는 $5\times{5}$ PWR 봉다발에서의 난류유동 측정)

  • Yang, Sun-Kyu;Chung, Heung-June;Chun, Se-Young;Chung, Moon-Ki
    • Nuclear Engineering and Technology
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    • v.24 no.3
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    • pp.263-273
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    • 1992
  • The study on the velocity distribution and the pressure drop characteristic of the nuclear fuel assembly is of importance for the thermal hydraulic design and safety analysis. The purpose of this experimental study is to investigate the hydraulic mixing behind the different kinds of spacer grids in the now or rod bundles. In this study, the detailed hydraulic characteristics in subchannels of 5$\times$5 PWR(Pressurized Water Reactor) rod bundles were measured using one-component He-Ne LDV(Laser Doppler Velocimeter). Measurements of the axial velocity, turbulent intensities and pressure drops were peformed Lateral velocity, turbulent intensities and Reynolds shear stress were also measured by adjust-ing LDV alignment. Friction factors in rod bundles and loss coefficients for spacer grids were evaluated from the measured pressure drops. Hydraulic mixing performance for different kinds of spacer grids could be investigated by estimating the turbulent cross-flow mixing rates between neighboring subchannels.

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Fabrication of Laminated Multi-layer Flexible Substrate with Cu/Sn Via (Cu/Sn 비아를 적용한 일괄적층 방법에 의한 다층연성기판의 제조)

  • Lee H. J.;Yu Jin
    • Journal of the Microelectronics and Packaging Society
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    • v.11 no.4 s.33
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    • pp.1-5
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    • 2004
  • A multi-layer flexible substrate is composed of copper(Cu)/polyimide that are known as good electrical conductivity, and low dielectric constant, respectively. In this study. conductor line of $5{\mu}m$-pitch was successfully fabricated without non-uniform pattern shape by electroplating copper and coating polyimide on patterned stainless steel. For multi-layer flexible substrate, via holes were drilled by UV laser and filled with electroplating copper and tin. And then, the PI layer with vias and conductor lines was stripped from stainless steel substrate. The PI layers were laminated at once with careful alignment between layers. Solid state reaction between tin and copper during lamination formed the intermetallic compounds of $Cu_6Sn_5$($\eta$-phase) and $Cu_3Sn$($\epsilon$-Phase) and achieved a complete inter-connection by vertically positioning the plugged via holes on via pad. The via formation process has several advantages; such as better electrical property and lower cost than V type via and paste via.

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Radial displacement of clinical target volume in node negative head and neck cancer

  • Jeon, Wan;Wu, Hong-Gyun;Song, Sang-Hyuk;Kim, Jung-In
    • Radiation Oncology Journal
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    • v.30 no.1
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    • pp.36-42
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    • 2012
  • Purpose: To evaluate the radial displacement of clinical target volume in the patients with node negative head and neck (H&N) cancer and to quantify the relative positional changes compared to that of normal healthy volunteers. Materials and Methods: Three node-negative H&N cancer patients and five healthy volunteers were enrolled in this study. For setup accuracy, neck thermoplastic masks and laser alignment were used in each of the acquired computed tomography (CT) images. Both groups had total three sequential CT images in every two weeks. The lymph node (LN) level of the neck was delineated based on the Radiation Therapy Oncology Group (RTOG) consensus guideline by one physician. We use the second cervical vertebra body as a reference point to match each CT image set. Each of the sequential CT images and delineated neck LN levels were fused with the primary image, then maximal radial displacement was measured at 1.5 cm intervals from skull base (SB) to caudal margin of LN level V, and the volume differences at each node level were quantified. Results: The mean radial displacements were 2.26 (${\pm}1.03$) mm in the control group and 3.05 (${\pm}1.97$) in the H&N cancer patients. There was a statistically significant difference between the groups in terms of the mean radial displacement (p = 0.03). In addition, the mean radial displacement increased with the distance from SB. As for the mean volume differences, there was no statistical significance between the two groups. Conclusion: This study suggests that a more generous radial margin should be applied to the lower part of the neck LN for better clinical target coverage and dose delivery.

Design and Verification of Far-field measurement system for W-band Millimeter-wave Antenna (밀리미터파(W대역) 안테나 측정을 위한 원-전계 측정 시스템 설계 및 검증)

  • Baek, Jong-Gyun;Hwang, In-June;Cho, Chi-Hyun;Joo, Joung-Myoung;Joo, Ji-Han;Park, Jong-Kuk;Kim, Jaesik;Kim, Sosu
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.22 no.2
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    • pp.43-50
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    • 2022
  • In this paper, we designed and verified a Far-field measurement system for measuring the performance of an antenna operating in millimeter wave(W-band). For the antenna test, a measurement system should be selected according to the type of antenna, measurement items, measurement environment and period, etc. In the case of near-field measurement, it takes a lot of time because the number of measurement items increases when the antenna has multiple channels or various beams. Such an antenna can reduce the measurement time through Far-field measurement, and only necessary measurement items can be measured. Therefore, this study secured precise alignment of the far-field measurement system using a high-power laser and improved the measurement accuracy by applying a double amplifier system. The designed system was built in the anechoic chamber and verified by comparison with the verified Near-field measurement system.

DEVELOPMENT OF LIGHTWEIGHT OPTICAL TELESCOPE KIT USING ALUMINUM PROFILE AND ISOGRID STRUCTURE

  • Park, Woojin;Lee, Sunwoo;Han, Jimin;Ahn, Hojae;Ji, Tae-Geun;Kim, Changgon;Kim, Dohoon;Lee, Sumin;Kim, Young-Jae;Kim, Geon-Hee;Kim, Junghyun;Kim, Ilhoon;Pak, Soojong
    • Journal of The Korean Astronomical Society
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    • v.55 no.1
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    • pp.11-22
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    • 2022
  • We introduce the Transformable Reflective Telescope (TRT) kit that applies an aluminum profile as a base plate for precise, stable, and lightweight optical system. It has been utilized for optical surface measurements, developing alignment and baffle systems, observing celestial objects, and various educational purposes through Research & Education projects. We upgraded the TRT kit using the aluminum profile and truss and isogrid structures for a high-end optical test device that can be used for prototyping of precision telescopes or satellite optical systems. Thanks to the substantial aluminum profile and lightweight design, mechanical deformation by self-weight is reduced to maximum 67.5 ㎛, which is an acceptable misalignment error compared to its tolerance limits. From the analysis results of non-linear vibration simulations, we have verified that the kit survives in harsh vibration environments. The primary mirror and secondary mirror modules are precisely aligned within 50 ㎛ positioning error using the high accuracy surface finished aluminum profile and optomechanical parts. The cross laser module helps to align the secondary mirror to fine-tune the optical system. The TRT kit with the precision aluminum mirror guarantees high quality optical performance of 5.53 ㎛ Full Width at Half Maximum (FWHM) at the field center.

Manufacturing and Characteristic Evaluation of Free space Optical Communication Devices in 5G Mobile Base Stations for Emergency Disaster Response (긴급재난 대응용 5G 이동 기지국을 위한 대기공간 광통신 장치의 제작과 특성평가)

  • Jin-Hyeon Chang
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.23 no.5
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    • pp.131-138
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    • 2023
  • In this paper, a free space optical communication device that can be used in a mobile base station of several km or less was fabricated and its characteristics were investigated. To overcome the loss due to atmospheric transmission, an optical fiber amplifier (EDFA) with an output of 23 dBm or more was used. In order to increase the focusing speed and miniaturization of the laser beam, an optical lens was manufactured, and a transmission lens was designed to have beam divergence within the range of 1.5 to 1.8 [mrad]. A PT module that controls PAN/TILT was fabricated in order to reduce pointing errors and effective automatic alignment between transceiver devices. In this study, Reed-Solomon (RS) code was used to maintain the transmission quality above a certain level. It was manufactured to be able to communicate at a wireless distance of 300m in a weather situation with visibility of 300m. For performance measurement, it was measured using BERT and eye pattern analyzer, and it was confirmed that BER can be maintained at 2.5Gbps.

Efficiency Evaluation of CT Simulator QA Phantom (전산화 단층촬영 모의치료기 정도관리 팬텀의 유용성 평가)

  • Hwang, Se-Ha;Min, Je-Sun;Lee, Jae-Hee;Park, Heung-Deuk
    • The Journal of Korean Society for Radiation Therapy
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    • v.21 no.2
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    • pp.89-95
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    • 2009
  • Purpose: The purpose is to evaluate efficiency of the CT simulator QA phantom manufactured for daily QA. Materials and Methods: We made holes ($1{\times}100{\times}1\;mm$) to verify accuracy between image and real measurement in polystyrene phantom and made 1 mm holes to verify table movement accuracy at superior and inferior 100 mm to the center of the phantom and inserted radiopacity material. To evaluate laser alignment, we made cross mark on the right and left side at phantom and to evaluate CT number accuracy we made 3 cylindrical holes and inserted equivalence material of bone, water, air in them. After CT scanning the phantom, We evaluated accuracy between image and real measurement, accuracy of table movement, laser, and CT number using exposed image. Results: It was measured that the accuracy between image and real measurement was ${\pm}0.3\;mm$, table movement accuracy was ${\pm}0.3\;mm$, laser accuracy was ${\pm}0.5\;mm$ from 7th January to 7th March in 2008 as within the reference point ${\pm}1\;mm$. In the CT number accuracy of bone was ${\pm}10\;HU$, air was ${\pm}5\;HU$, water was ${\pm}5\;HU$ as within the reference point is ${\pm}10\;HU$. Conclusion: We was able to perform CT simulator QA and laser equipment QA more conveniently and fast using manufactured phantom at the same time. We will be able to make more accurate treatment plan that added to QA procedures using images at previous daily QA.

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Holographic phase gratings in back- and frontlights for LCD's

  • Bastiaansen, C.W.M.;Heesch, C. van;Broer, D.J.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2006.08a
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    • pp.421-421
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    • 2006
  • The light and energy-efficiency of classical liquid crystal displays is notoriously poor due to the use of absorption-based linear polarisers and colour filters. For instance, the light efficiency of PVAL polarisers is typically between 40 and 45 % and the colour filters have a typical efficiency below 35 % which results in a total light and energy-efficiency of the display below 10 %. In the past, a variety of polarizers were developed with an enhanced efficiency in generating linearly polarized light. Typically, these polarizers are based on the polarisationselective reflection, scattering or refraction of light i.e. one polarisation direction of light is directly transmitted to the LCD/viewer and the other polarization direction of light is depolarised and recycled which results in a typical efficiency for generating linearly polarized light of 70-85 %. Also, special colour filters have been proposed based on chiral-nematic reactive mesogens which increase the efficiency of generating colour. Despite the enormous progress in this field, a need persists for improved methods for generating polarized light and colour based on low cost optical components with a high efficiency. Here, the use of holographic phase gratings is reported for the generation of polarized light and colour. The phase grating are recorded in a photopolymer which is coated onto a backor frontlight for LCDs. Typically the recording is performed in the transmisson mode or in the waveguiding mode and slanted phase gratings are generated with their refractive index modulation at an angle between 20o and 45o with the normal of the substrate. It is shown that phase gratings with a high refractive index modulation and a high efficiency can be generated by a proper selection of the photopolymer and illumination conditions. These phase gratings coupleout linearly polarized light with a high contrast (> 100) and the light is directed directly to the LCD/viewer without the need for redirection foils. Dependent on the type of phase grating, the different colours are coupled-out at a slightly different angle which potentially increases the efficiency of classical colour filters. Moreover, the phase gratings are completely transparent in direct view which opens the possibility to use them in frontlights for LCDs. Holographic polarization gratings posses a periodic pattern in the polarization state of light (and not in the intensity of light). A periodic pattern in the polarization direction of linearly polarized light is obtained upon interference of two circularly polarized laser beams. In the second part of the lecture, it is shown that these periodic polarization patterns can be recorded in a linear photo-polymerizable polymer (LPP) and that such an alignment layer induces a period rotation in the director of (reactive and non-reactive) liquid crystals. By a proper design, optical components can be produced with only first order diffraction and with a very high efficiency (>0.98). It is shown that these diffraction gratings are potentially useful in projection displays with a high brightness and energy efficiency

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