• Title/Summary/Keyword: kVp test

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Production of the Antiserum against Recombinant Envelop Protein, rVP466 for the Neutralization of White Spot Syndrome Virus (WSSV) (흰반점바이러스(WSSV)의 중화를 위한 재조합단백질 rVP466의 항혈청 생산)

  • Gong, Soo-Jung;Kim, Yeong-Jin;Choi, Mi-Ran;Kim, Sung-Koo
    • Journal of Life Science
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    • v.20 no.10
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    • pp.1427-1432
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    • 2010
  • This study was carried out to evaluate neutralization effects against WSSV using antiserum produced from recombinant envelop protein, rVP466 of WSSV. The VP466 gene of WSSV was cloned into pCold I expression vector and rVP466 was expressed in E. coli RIPL. The antiserum against rVP466 was produced in white rabbits (New Zealand white rabbit). The specific immunoreactivity to the antigen, rVP466, was confirmed by Western blot. The constant amounts of WSSV at $1{\times}10^4$ diluted stocks were mixed with various antiserum concentrations and then injected to the muscle of shrimp, Penaeus chinensis, for the neutralization challenge. The shrimps challenged with WSSV as a positive control and those with the mixture of WSSV and preimmune serum as a preimmune control showed 100% cumulative mortality at 17 days post challenge and 83% at 25 days post challenge, respectively. The shrimps challenged with 3 different mixtures of WSSV and rVP466 antiserum at ratios of 1:0.01, 1:0.1 and 1:1 showed 73%, 53% and 46% cumulative mortalities at 25 days post challenge, respectively. These results indicated that WSSV could be neutralized by the rVP466 antiserum. These results suggest that envelop protein VP466 is involved in the initial step of WSSV infection in shrimp.

Change of PAE according to Detector Measurement Method (검출기 측정방법에 따른 PAE값의 변화)

  • Im, In-Chul
    • The Journal of the Korea Contents Association
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    • v.10 no.6
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    • pp.307-311
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    • 2010
  • The aim of this study is to investigate PAE, as the result of the test of kVp accuracy, according to detector measurement method. Based on the indicated value of 70kVp, each distance between a focus and a kVp meter was 100cm, 80cm and 60cm and the angle of X-ray tube was set on $5^{\circ},\;10^{\circ},\;15^{\circ},\;20^{\circ},\;25^{\circ},\;30^{\circ}$. Each indicated value, 60kVp, 70kVp, 80kVp, 90kVp and 100 kVp, was used compare Small focus with Large focus. As a result, PAE on the side of cathode was higher than it on the side of anode in the case of 100cm and PAE on the side of anode was higher in the case of 80cm and 60cm. The coefficient rate was stable both the side of cathode and anode in the case of 100cm and it was fluctuated in the case of 80cm and 60cm. PAE in the case of Small focus was higher than Large focus and it was disproportionate to an indicated value. Error rate was in inverse proportion to the indicated value.

Dose and Image Evaluation according to Changes in Tube Voltage during Chest X-ray Examination according to Automatic Exposure Control (자동노출제어장치 유·무에 따른 흉부 후·전방향 검사 시 관전압 변화에 따른 선량 및 영상평가)

  • Young-Cheol, Joo;Dong-Hee, Hong
    • Journal of the Korean Society of Radiology
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    • v.16 no.7
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    • pp.871-877
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    • 2022
  • This study was conducted to improve the problems of exposure dose and image reading applied to patients due to the incorrect use of AEC during chest radiography. Images were acquired by dividing the case where AEC was used as the test condition and the case where AEC was not used. As a result of the study, the dose was reduced by 1.17% in 110 kVp without AEC than with AEC, 17.2% decrease at 100 kVp, 30.19% decrease at 90 kVp, and 46.45% decrease at 80 kVp. There was a significant difference in the statistical values according to the tube voltage change in the lung, trachea, and heart SNR average values with AEC and without AEC 110 kVp, but the difference in image quality was insignificant in actual images. When AEC was not applied at the same tube voltage, the dose could be reduced by 17.2% while maintaining the image quality similar to that of with AEC at 100 kVp without AEC. Therefore, rather than relying on AE conditions during chest radiographic examination, it is considered that the conditions should be considered for the examination while lowering the dose by selecting an appropriate tube voltage.

Image Evaluation and Exposure Dose with the Application of Tube Voltage and Adaptive Statistical Iterative Reconstruction of Low Dose Computed Tomography (저 선량 전산화단층촬영의 관전압과 적응식 통계적 반복 재구성법 적용에 따른 영상평가 및 피폭선량)

  • Moon, Tae-Joon;Kim, Ki-Jeong;Lee, Hye-Nam
    • Journal of radiological science and technology
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    • v.40 no.2
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    • pp.261-267
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    • 2017
  • The study has attempted to evaluate and compare the image evaluation and exposure dose by respectively applying filter back projection (FBP), the existing test method, and adaptive statistical iterative reconstruction (ASIR) with different values of tube voltage during the low dose computed tomography (LDCT). With the image reconstruction method as basis, chest phantom was utilized with the FBP and ASIR set at 10%, 20% respectively, and the change of tube voltage (100 kVp, 120 kVp). For image evaluation, back ground noise, signal-noise ratio (SNR) and contrast-noise ratio (CNR) were measured, and, for dose assessment, CTDIvol and DLP were measured respectively. In terms of image evaluation, there was significant difference in ascending aorta (AA) SNR and inpraspinatus muscle (IM) SNR with the different amount of tube voltage (p < 0.05). In terms of CTDIvol, the measured values with the same tube voltage of 120 kVp were 2.6 mGy with no-ASIR and 2.17 mGy with 20%-ASIR respectively, decreased by 0.43 mGy, and the values with 100 kVp were 1.61 mGy with no-ASIR and 1.34 mGy with 20%-ASIR, decreased by 0.27 mGy. In terms of DLP, the measured values with 120 kVp were $103.21mGy{\cdot}cm$ with no-ASIR and $85.94mGy{\cdot}cm$ with 20%-ASIR, decreased by $17.27mGy{\cdot}cm$ (about 16.7%), and the values with 100 kVp were $63.84mGy{\cdot}cm$ with no-ASIR and $53.25mGy{\cdot}cm$ with 20%-ASIR, a decrease by $10.62mGy{\cdot}cm$ (about 16.7%). At lower tube voltage, the rate of dose significantly decreased, but the negative effects on image evaluation was shown due to the increase of noise.

A study of image evaluation and exposure dose with the application of Tube Voltage and ASIR of Low dose CT Using Chest Phantom (흉부 Phantom을 이용한 Low Dose CT의 관전압과 ASIR(Adaptive Statistical Iterative Reconstruction)적용에 따른 영상평가 및 피폭선량에 관한 연구)

  • Hwang, Hyeseong;Kim, Nuri;Jeong, Yoonji;Goo, Eunhoe;Kim, Kijeong
    • Korean Journal of Digital Imaging in Medicine
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    • v.16 no.2
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    • pp.9-14
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    • 2014
  • Purpose: The purpose of this study has attempted to evaluate and compare the image evaluation and exposure dose by respectively applying Filtered Back Projection(FBP), the existing test method, and Adaptive Statistical Iterative Reconstruction(ASIR) with different values of tube voltage during the Low Dose Computed Tomography(LDCT). Materials and Methods: With the image reconstruction method as basis, Chest Phantom was utilized with the FBP and ASIR set at 10%, 20% respectively, and the change of Tube Voltage (100kVp, 120kVp). For image evaluation, Back ground noise, Signal to Noise ratio(SNR) and Contrast to Noise ratio(CNR) were measured, and, for dose evaluation, CTDIvol and DLP were measured respectively. The statistical analysis was tested with SPSS(ver. 22.0), followed by ANOVA Test conducted after normality test and homogeneity test. (p<0.05). Results: In terms of image evaluation, there was no outstanding difference in Ascending Aorta(AA) SNR and Infraspinatus Muscle(IM) SNR with the different values of ASIR application(p<0.05), but a significant difference with the different amount of tube voltage(p>0.05). Also, there wasn't noticeable change in CNR with ASIR and different amount of Tube Voltage (p<0.05). However, in terms of dose evaluation, CTDIvol and DLP showed contrasting results(p<0.05). In terms of CTDIvol, the measured values with the same tube voltage of 120kVp were 2.6mGy with No-ASIR and 2.17mGy with 20%-ASIR respectively, decreased by 0.43mGy, and the values with 100kVp were 1.61mGy with No-ASIR and 1.34mGy with 20%-ASIR, decreased by 0.27mGy. In terms of DLP, the measured values with 120kVp were $103.21mGy{\cdot}cm$ with No-ASIR and $85.94mGy{\cdot}cm$ with 20%-ASIR, decreased by $17.27mGy{\cdot}cm$(about 16.7%), and the values with 100kVp were $63.84mGy{\cdot}cm$ with No-ASIR and $53.25mGy{\cdot}cm$ with 20%-ASIR, a decrease by $10.62mGy{\cdot}cm$(about 16.7%). Conclusion: At lower tube voltage, the rate of dose significantly decreased, but the negative effects on image evaluation was shown due to the increase of noise. For the future, through the result of the experiment, it is considered that the method above would be recommended for follow-up patients or those who get health checkup as long as there is no interference on the process of diagnosis due to the characteristics of Low Dose examination.

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An Improvement Video Search Method for VP-Tree by using a Trigonometric Inequality

  • Lee, Samuel Sangkon;Shishibori, Masami;Han, Chia Y.
    • Journal of Information Processing Systems
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    • v.9 no.2
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    • pp.315-332
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    • 2013
  • This paper presents an approach for improving the use of VP-tree in video indexing and searching. A vantage-point tree or VP-tree is one of the metric space-based indexing methods used in multimedia database searches and data retrieval. Instead of relying on the Euclidean distance as a measure of search space, the proposed approach focuses on the trigonometric inequality for compressing the search range, which thus, improves the search performance. A test result of using 10,000 video files shows that this method reduced the search time by 5-12%, as compared to the existing method that uses the AESA algorithm.

A Study on the Dose Reduction Method for Temporal Bone HRCT Scan (관자뼈 HRCT 스캔 시 선량감소 방법에 관한 연구)

  • Joon Yoon;Hyeon-Ju Kim
    • Journal of the Korean Society of Radiology
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    • v.17 no.7
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    • pp.1041-1047
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    • 2023
  • Temporal bone CT, which is a high-resolution CT, uses a high tube voltage and a thin section thickness, so the scan dose is higher than that of adjacent areas. Accordingly, we applied changes to the reconstruction algorithm among the test conditions to find an algorithm with excellent sensitivity to lesions while reducing the test dose, and investigated its significance and the possibility of providing basic clinical data. As a result, when the tube voltage was lowered to 100 kVp and applied, the dose was reduced by about 35.6%, and when the definition algorithm was applied to the raw data acquired at 100 kVp, the SNR and CNR were excellent, and a statistically significant difference was shown when compared to other algorithms(p<0.05). And as a result of comparing structural similarity, the SSIM index was analyzed as 0.776, 0.813, and 0.741 for each ROI. Therefore, we believe that applying algorithm changes to temporal bone CT scans can partially reduce the dose generated from CT scans and are very meaningful in terms of basic clinical data.

Monoclonal antibodies against porcine group C rotavirus VP6 (돼지 group C 로타바이러스 VP6 특이 단클론항체)

  • Yoon, Young-Sim;Lee, Seung-Chul;Woo, Sang-Kyu;Cho, Kyoung-Oh;Kang, Shien-Young
    • Korean Journal of Veterinary Service
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    • v.35 no.3
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    • pp.175-182
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    • 2012
  • Rotaviruses have been known to be a major etiological agent of gastroenteritis in both infants and young animals. Subsequently new rotaviruses, which were morphologically indistinguishable but antigenically and electrophoretically distinct with each other, were reported from several animals throughout world including Korea. These new rotaviruses were named as non-group A or group B or group C rotaviruses and so on. It has been very difficult to isolate and grow the non-group A rotaviruses in vitro, and this has greatly limited the characterizations of non-group A rotaviruses and serological studies. In this study, monoclonal antibodies (MAbs) against porcine non-group A rotavirus were produced and characterized. The VP6 gene of porcine group C rotavirus Korean isolate(#06-52-1) was cloned and expressed. For expression of VP6 gene, baculovirus expression system was applied. The VP6 gene and expressed protein in the recombinant virus were confirmed by polymerase chain reaction (PCR), indirect fluorescence antibody (IFA) test and Western blot, respectively. The expressed VP6 was used for MAbs production. The MAbs produced in this study would be promising as diagnostic reagents for detection of group C rotavirus infection.

A Comparison of Density and Patient Doses According to kVp and mAs Changes in General Radiography (일반촬영에서 kVp와 mAs의 변화에 따른 농도와 환자 선량 비교)

  • Kang, Eun Bo
    • Journal of the Korean Society of Radiology
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    • v.13 no.7
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    • pp.987-994
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    • 2019
  • Low energy x-rays that occur in the low tube voltage radiography of general radiography are absorbed strongly in the body and do not aid image quality enhancement. This study maintains titer in general radiography while using tube current that are proportional to density and the tube voltage 15% principle according to density to reduce patient exposure doses, and area doses and entrance surface doses were measured to compare patient exposure doses. In hand, knee, abdomen, and skull radiography, kVp was increased to 115% and mAs was decreased to 50% and kVp was decreased to 85% while mAs was increased to 200% and area doses and entrance surface doses were measured to compare relative doses. Also, 5 places in each image were set, density was measured, and Kruskal wallis H test was conducted to observe significance probabilities between groups. To fix density, kVp was increased to 115% and mAs was decreased to 50% and after measurements of mean area doses and entrance surface doses were made by each part, each decreased to 58.68% and 59.85% when standard doses were set to 100%, and each increased to 147.28% and 159.9% when kVp was decreased to 85% and mAs was increased to 200%. Comparisons of density changes showed that hand, knee, abdomen, and skull radiography all displayed significance probabilities>0.05, showing no changes in concentration. Radiography that increases kVp and lowers mAs through reasonable calculations within ranges that don't affect resolution and contrast seems to be a simple way to decrease patient exposure doses.

Development and Performance Comparison of Silicon Mixed Shielding Material (실리콘 혼합 차폐체의 개발과 성능비교)

  • Hoi-Woun Jeong;Jung-Whan Min
    • Journal of radiological science and technology
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    • v.46 no.3
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    • pp.187-195
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    • 2023
  • A shield was made by mixing materials such as bismuth(Bi) and barium(Ba) with silicon to evaluate its shielding ability. Bismuth was made into a shield by mixing a bismuth oxide(Bi2O3) colloidal solution and a silicon base and applied to a fibrous fabric, and barium was made by mixing lead oxide(PbO) and barium sulfate(BaSO4) with a silicon curing agent and solidifying it to make a shield. The test was conducted according to the lead equivalent test method for X-ray protective products of the Korean Industrial Standard. The experiment was conducted by increasing the shielding body one by one from the test condition of 60 kVp, 200 mA, 0.1sec and 100 kVp, 200 mA, 0.1 sec. At 60 kVp, 2 lead oxide-barium sulfate shields, 2 bismuth oxide 1.5 mm shields, and 5 bismuth oxide 0.3 mm shields showed shielding ability equal to or higher than that of lead 0.5 mm. At 100 kVp, 2 lead oxide-barium sulfate shields and 2 bismuth oxide 1.5 mm shields showed shielding ability equal to or higher than that of lead 0.5 mm. It was confirmed that when using 2 pieces of lead oxide-barium sulfate and 1.5 mm of bismuth oxide, respectively, it has shielding ability equivalent to that of lead. Bismuth oxide and lead oxide-barium sulfate are lightweight and have excellent shielding ability, thus they have excellent properties to be used as an apron for radiation protection or other shielding materials.