Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Monoclonal antibodies against porcine group C rotavirus VP6

돼지 group C 로타바이러스 VP6 특이 단클론항체

  • Received : 2012.08.16
  • Accepted : 2012.09.03
  • Published : 2012.09.30
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Abstract

Rotaviruses have been known to be a major etiological agent of gastroenteritis in both infants and young animals. Subsequently new rotaviruses, which were morphologically indistinguishable but antigenically and electrophoretically distinct with each other, were reported from several animals throughout world including Korea. These new rotaviruses were named as non-group A or group B or group C rotaviruses and so on. It has been very difficult to isolate and grow the non-group A rotaviruses in vitro, and this has greatly limited the characterizations of non-group A rotaviruses and serological studies. In this study, monoclonal antibodies (MAbs) against porcine non-group A rotavirus were produced and characterized. The VP6 gene of porcine group C rotavirus Korean isolate(#06-52-1) was cloned and expressed. For expression of VP6 gene, baculovirus expression system was applied. The VP6 gene and expressed protein in the recombinant virus were confirmed by polymerase chain reaction (PCR), indirect fluorescence antibody (IFA) test and Western blot, respectively. The expressed VP6 was used for MAbs production. The MAbs produced in this study would be promising as diagnostic reagents for detection of group C rotavirus infection.

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References

  1. Anderson EJ, Weber SG. 2004. Rotavirus infection in adults. Lancet Infect Dis 4: 91-99. https://doi.org/10.1016/S1473-3099(04)00928-4
  2. Bridger JC. 1987. Novel rotaviruses in animals and man. Ciba Found Symp 128: 5-23.
  3. Collins PJ, Martella V, O'Shea H. 2008. Detection and characterization of group C rotaviruses in asymptomatic piglets in Ireland. J Clin Microbiol 46: 2973-2979. https://doi.org/10.1128/JCM.00809-08
  4. Estes MK, Cohen J. 1989. Rotavirus gene structure and function. Microbiol Rev 53: 410-449.
  5. Estes MK, Crawford SE, Penaranda ME, Petrie BL, Burns JW, Chan WK, Ericson B, Smith GE, Summers MD. 1987. Synthesis and immunogenicity of the rotavirus major capsid antigen using a baculovirus expression system. J Virol 61: 1488-1494.
  6. Jeong YJ, Park SI, Hosmillo M, Shin DJ, Chun YH, Kim HJ, Kwon HJ, Kang SY, Woo SK, Park SJ, Kim GY, Kang MI, Cho KO. 2009. Detection and molecular characterization of porcine group C rotaviruses in South Korea. Vet Microbiol 138: 217-224. https://doi.org/10.1016/j.vetmic.2009.03.024
  7. Jiang BM, Saif LJ, Kang SY, Kim JH. 1990. Biochemical characterization of the structural and nonstructural polypeptides of a porcine group C rotavirus. J Virol 64: 3171-3178.
  8. Kang SY, Saif LJ, Miller KL. 1989. Reactivity of VP4-specific monoclonal antibodies to a serotype 4 porcine rotavirus with distinct serotypes of human(symptomatic and asymptomatic) and animal rotaviruses. J Clin Microbiol 27: 2744-2750.
  9. Kim YJ, Chang KO, Straw B, Saif LJ. 1999. Characterization of group C rotaviruses associated with diarrhea outbreaks in feeder pigs. J Clin Microbiol 37: 1484-1488.
  10. Madore HP, Estes MK, Zarley BH, Parsons DD, Greiner S, Smith R, Jiang B, Corsaro B, Barniak V, Crawford S, Conner ME. 1999. Biochemical and immunologic comparison of virus-like particles for a rotavirus subunit vaccine. Vaccine 17: 2461-2471. https://doi.org/10.1016/S0264-410X(98)00319-3
  11. Ojeh CK, Jiang BM, Tsunemitsu H, Kang SY, Weilnau PA, Saif LJ. 1991. Reactivity of monoclonal antibodies to the 41-kilodalton protein of porcine group C rotavirus with homologous and heterologous rotavirus serogroups in immunofluorescence tests. J Clin Microbiol 29: 2051-2055.
  12. Orlik O, Altaner C. 1988. Modification of hybridoma technology which improve the yield of monoclonal antibody producing cells. J Immunol Methods 115: 55-59. https://doi.org/10.1016/0022-1759(88)90309-2
  13. Parashar UD, Hummelman EG, Bresee JS, Miller MA, Glass RI. 2003. Global illness and deaths caused by rotavirus disease in children. Emerg Infect Dis 9: 565-572. https://doi.org/10.3201/eid0905.020562
  14. Park SI, Jeong YJ, Kim HJ, Park JG, Kang SY, Woo SK, Kim CH, Jung CH, Kang MI, Cho KO. 2011. Genetically diverse group C rotaviruses cause sporadic infection in Korean calves. J Vet Med Sci 73: 479-482. https://doi.org/10.1292/jvms.10-0280
  15. Peralta A, Molinari P, Taboga O. 2009. Chimeric recombinant rotavirus- like particles as a vehicle for the display of heterologous epitopes. J Virol 6: 192-201. https://doi.org/10.1186/1743-422X-6-192
  16. Rodger SM, Bishop RF, Holmes IH. 1982. Detection of a rotavirus- like agent associated with diarrhea in an infant. J Clin Microbiol 16: 724-726.
  17. Saif LJ, Terrett LA, Miller KL, Cross RF. 1988. Serial propagation of porcine group C rotavirus (pararotavirus) in a continuous cell line and characterization of the passaged virus. J Clin Microbiol 26: 1277-1282.
  18. Snodgrass DR, Herring AJ, Campbell I, Inglis JM, Hargreaves FD. 1984. Comparison of atypical rotaviruses from calves, piglets, lambs and man. J Gen Virol 65: 909-914. https://doi.org/10.1099/0022-1317-65-5-909
  19. Thouless ME, Beards GM, Flewett TH. 1982. Serotyping and subgrouping of rotavirus strains by the ELISA test. Arch Virol 73: 219-230. https://doi.org/10.1007/BF01318076
  20. Tosser G, Labbe M, Bremont M, Cohen J. 1992. Expression of the major capsid protein VP6 of group C rotavirus and synthesis of chimeric single-shelled particles by using recombinant baculoviruses. J Virol 66: 5825-5831.
  21. Towbin H, Staechelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354. https://doi.org/10.1073/pnas.76.9.4350
  22. Yang DK, Kweon CH, Kim BH, Lim SI, Kwon JH, Kim SH, Song JY, Han HR. 2005. Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice. J Vet Sci 6: 125-133.
  23. Yolken RH, Barbour B, Wyatt RG, Kalica AR, Kapikian AZ, Chanock RM. 1978. Enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species. Science 201: 259-262. https://doi.org/10.1126/science.208150

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