• Journal of Nutrition and Health
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• 제35권6호
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• pp.635-642
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• 2002

This study was performed to investigate the effects of isoflavone consumption on plasma and liver lipid profiles and immune responses in Sprague-Dawley male rats. Experimental animals fed isoflavone at various doses for 4 weeks (0, 1095, 2190, 4380 isoflavone mg/kg diet). Exposure to isoflavone decreased the food consumption and final body weights of rats without decreasing the relative weights of organs, hemoglobin and hematocrit. And the plasma cholesterol and LDL-cholesterol, liver total lipid, cholesterol and triglyceride concentrations were significantly decreased by isoflavone intakes. The absolute and relative weights of thymus were significantly decreased in groups fed isoflavone than in control. Also splenocyte proliferations with Con A or PHA were decreased according to isoflavone consumption in rats, although there was not significant. These results demonstrate that isoflavone intakes significantly improve lipid profiles in plasma and liver. But the effects of isoflavone intakes on immune responses are needed further experiments.

• 한국식품과학회지
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• 제34권5호
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• pp.916-918
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• 2002

산가수분해시 산의 농도가 탈지대두박의 isoflavone 양에 미치는 영향을 조사하였다. 측정한 isoflavone은 daidzin, genistin 등 glucosides와 daidzein, genistein 등 aglycons이었다. 산가수분해는 탈지대두박 분말에 0.25 N-3.00 N HCl을 가하고 $95^{\circ}C$에서 4시간 가열하였다. 그 결과 총 isoflavone은 0.25 N-1.0 N HCl의 범위에서 가열이 진행되면서 증가하여 2시간 후에는 약 40% 증가함을 보였다. 그러나 그 이상의 농도에서는 현저한 감소가 있어 4시간 후에는 52%의 감소가 있었다. 이 결과는 낮은 HCl 농도에서의 증가는 isoflavone isomer가 isoflavone으로 전환되었음을 보여주고 높은 농도에서의 감소는 isoflavone이 분해됨을 보여주고 있다. 가열 중 각각의 isoflavone의 변화는 전체적으로 glucosides는 감소하고 aglycons는 증가하는 경향이었다. 특히 2 N HCl에서의 60분 반응시 daidzin과 genistin, glycitin 등 glucosides는 검출되지 않았다.

• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.57-62
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• 2008

Isoflavone content in various parts of six soybean cultivars and soybean sprout during germination was analyzed by high performance liquid chromatography. The parts analyzed were seed coat, cotyledon, and axis for seeds and whole sprout, root, hypocotyl, and cotyledon for sprout. Two cultivars, Aga3 which is known to have the smallest seed size and the highest isoflavone content among the Korean soybean cultivars and Pungsannamulkong which is the most widely being used as soy-sprout, were selected for sampling from 1 to 10 days after germination. At the same weight, the order of isoflavone content increased from seed coat to cotyledon to axis. The highest total isoflavone(isoflavone$\times$dry weight) content was observed in the cotyledon and the lowest in the seed coat. The cotyledon of the Aga3 variety had the highest total isoflavone content and the lowest was measured in the Pungsannamulkong variety. The highest total isoflavone content, $10,788{\mu}g/g$, was observed in whole sprouts(cotyledon+hypocotyl+root) on day 7 for Aga3. After day 7, there was a decreasing trend in isoflavone content as the germination period increased. Total isoflavone content in the cotyledon of Aga3 significantly increased after seed germination, whereas the isoflavone content in the cotyledon of Pungsannamulkong decreased. However, total isoflavone content in the root of both varieties increased while isoflavone content in the hypocotyls decreased after seed germination.

• 한국작물학회지
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• 제51권spc1호
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• pp.174-178
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• 2006

본실험은 조생종인 하대두형 및 중국수집종 콩과 중숙${\sim}$만숙종의 국내 육성 종과의 isoflavone 함량변이를 비교하며, 파종기 이동 및 콩의 생육단계별 생육기간에 따른 isoflavone함량의 차이를 분석하고자 수행하였으며, 결과를 요약하면 다음과 같다. 1. 전체 isoflavone함량은 파종기 이동에 관련 없이 국내 수집종이 하대두형 및 중국수집 종에 비하여 놓았으며, 하대 두형콩과 중국수집 종과 비슷한 함량 분포를 나타냈다. 2. 파종기이동에 따른 총 isoflavone 함량은 파종기를 늦게 할수록 하대두형, 중국수집종 및 국내육성종 모두에서 증가하는 경향 이었다. 3. 콩의 개화기${\sim}$성숙기의 기간 및 생육일수가 길어질수록 isoflavone 함량도 높아지는 경향을 나타냈으며, 통계적으로도 부합되었다. 4. 콩의 isoflavone 함량은 입중이 무거울수록 함량이 낮아 졌으며, 상관계수는 4월${\sim}$5월 파종구에서 각각 -0.57, -0.51 및 -0.38로 분석되었다.

• Current Research on Agriculture and Life Sciences
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• 제26권
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• pp.55-62
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• 2008

콩나물로부터 고 isoflavone 함량의 콩 식품원료를 얻고자 콩나물 재배 시 빛을 쪼여주고, 재배한 콩나물의 저장온도를 달리한 조건에서 isoflavone 함량변이를 조사하였다. 공시 품종들의 7일 재배 후 저장하기 전 isoflavone 함량은 아가 3호>아가 1호>아가 2호>아가 4호>장기콩>보석콩>은하콩>풍산나물콩 순이었는데 아가 3호의 isoflavone 함량은 $4,619{\mu}g/g$이었다. 재배한 콩나물의 isoflavone 함량은 저장온도에 따라 품종별로 상이한 차이를 보였는데, 저장온도 $6^{\circ}C$와 $20^{\circ}C$에서 isoflavone 함량이 높은 반면 저장온도 $9^{\circ}C$와 $30^{\circ}C$에서는 대부분 콩 품종들의 isoflavone 함량은 낮았다. 저장일수에 따른 isoflavone의 함량은 품종들 간에 일정한 경향이 없었으나 대부분의 품종들은 저장기간 3일 이내에 가장 높은 isoflavone 함량을 보였고, 아가 4호와 장기콩은 저장기간 5일에서도 높은 isoflavone의 함량을 보였다. 저장기간과 온도처리를 하여 재배한 콩나물의 isoflavone 함량을 높이고자 한 결과 아가 3호를 포함하여 공시한 8품종 중 4품종에서 $20^{\circ}C$에서 하루 동안 저장했을 때 가장 높은 isoflavone의 함량을 나타내었다. 재배한 콩나물들 중 저장을 통해 가장 높은 isoflavone의 함량을 보인 품종은 아가 3호로써 저장후의 isoflavone 함량은 $11,705{\mu}g/g$이었다. 본 연구에서는 콩나물 저장기간 동안 수분공급이 지속적으로 이루어졌기 때문에 콩나물이 계속 성장한 것으로 생각된다. 따라서 저장기간 동안 온도처리를 달리하여 isoflavone의 함량을 증가시키는 보다 확실한 방법을 확립하기 위해서는 더 많은 연구가 이루어져야 할 것으로 생각되어진다.

• Archives of Pharmacal Research
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• 제26권5호
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• pp.426-431
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• 2003

This study was carried out to investigate the addition of water-soluble isoflavone into milk by means of microencapsulation technique. The yield of microencapsulation, sensory attributes, and capsule stability of water-soluble isoflavone microcapsules in milk were measured. Coating materials used was polyglycerol monostearate (PGMS), and core material was water-soluble isoflavone. The encapsulation yield of water-soluble isoflavone with PGMS was 67.2% when the ratio of coating material to core material was 15 : 1. The rate of water-soluble isoflavone release from capsules was 18, 19, and 25% when stored at 4,20, and $30^{\circ}C$ for 12 days in milk, respectively. In sensory evaluation, beany flavor and color of microencapsuled water-soluble isoflavone added milk were significantly different from uncapsuled water-soluble isoflavone added milk, however, bitterness was not significantly different. In vitro study, micro-capsules of water-soluble isoflavone in simulated gastric fluid with the range of 3 to 6 pHs were released 3.0∼15.0%, however, the capsules in simulated intestinal fluid with pH 7 were released 95.7% for 40 min incubation time. In conclusion, this study provided that PGMS as coating materials was suitable for the microencapsulation of water-soluble isoflavone, and the capsule containing milk was almost not affected with sensory attribute.

• Nutrition Research and Practice
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• 제16권sup1호
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• pp.134-146
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• 2022

BACKGROUND/OBJECTIVES: Accumulating evidence has shown the beneficial effects of isoflavone on health. There is limited information on the usual isoflavone intake for Koreans. This study examined the usual intake of total isoflavone and its major food sources in Koreans according to age and gender. SUBJECTS/METHODS: The dietary intake data of 21,271 participants aged 1 yrs and older from the Korea National Health and Nutrition Examination Survey (KNHANES) VII 2016-2018 were analyzed. The average isoflavone intake was estimated based on the 24-h dietary recall data in KNHANES and the isoflavone database from the Korea Rural Development Administration (RDA) and literatures. The usual isoflavone intake was estimated by applying the ratio of within- and between-participant variance estimated from the 2009 KNHANES data to the 7th KNHANES (2016-2018) data. The variance of the isoflavone intake was calculated using MIXTRAN macro with intake data for two days in the 2009 KNHANES. Complex sample analysis with stratified variables and integrated weights was conducted. RESULTS: The mean total isoflavone intake in the Korean population aged 1 yrs and older (n = 21,271) was 139.27 mg/d, which was higher than the usual intake of 47.44mg/d. Legumes were a major contributing food group (91%), with arrowroot being a major individual contributor to the isoflavone intake (67.2%), followed by 21.3% of soybean, 5.4% of bean sprouts, and 2.1% of tofu. The usual isoflavone intake was highest in the participants aged 50 to 64 yrs old and increased with age until 50 to 64 yrs and then decreased with further increases in age. The usual isoflavone intake of participants aged 65 yrs and older was higher for men than for women, showing gender differences. CONCLUSIONS: The usual dietary intake of isoflavone varied according to age and gender in the Korean population. This study showed that the usual isoflavone intake was lower than the average isoflavone intake. The difference between percentiles of the usual isoflavone intake was similarly smaller than the average intake. An estimation of average intake can be hindered by the occasional consumption of foods high in isoflavones, suggesting that the usual intake estimation method can be more appropriate. Further research will be needed to establish isoflavone dietary guidelines regarding the effects of isoflavone intake on health outcomes.

• 대한지역사회영양학회지
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• 제5권1호
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• pp.120-129
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• 2000

Very little is known about the relation between isoflavone intake and menopausal symptoms in Korean woman To find the effects and correlations between these factors, questionnaires(maternal factors, menopausal symptoms) anthropometric measurement, 24hr dietary recalls, and urinary isoflavones analysis were conducted in 100 postmenopausal women residing in the Kyunggi-do rural area. The average age hight and weight of the subject were 61.5 years, 153.6 cm and 56.2kg. The average age at menarche, menopause, and menstrual cycle were 16.7 years, 47.2 years, 29.8 years, respectively. Most of the postmenopausal women experienced mildly menopausal symptoms. The mean calorie, protein and calcium intake were 1417.6 kcal(74.7% of the Korean RDA) 53.2 g(88.6% of the Korean RDA) and 454.0mg(65.6% of the Korean RDA) respectively. The ration of energy from carbohydrate, protein, and fat was 68: 15: 17 The average isoflavone intake from soy foods was 27.27 mg. The major food source of the isoflavone were soybeans and soybean curd. The average urinary isoflavone excretion was 2. 78nmol/mg Cr and showed significant positive correlation with isoflavone intake. Isoflavone intake from soy foods had a negative correlation with the severity of menopausal symptoms. Therefore, soy foods which contain isoflavone may have a protective effect on menopausal symptoms of women in Korea.

• Journal of Nutrition and Health
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• 제36권7호
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• pp.667-674
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• 2003

The study was performed to investigate the effects of isoflavone and/or grape seed oil supplementation on serum and liver lipid profiles and bone strength in ovariectomized female rats. Female Spraque-Dawley rats were assigned into 4 groups of ovariectomized (O), isoflavone (0.085 mg/100 g b.w/day) in ovariectomized rats, grape seed oil in ovariectomized rats and isoflavone and/or grape seed oil in ovariectomized rats. After 8 weeks, biochemical profiles of serum, liver and bone were analyzed. Total food intakes, body weight gains and FER (food efficiency ratio) were not statistically significantly different among groups. Total cholesterol, triglyceride and LDL-cholesterol levels in serum were decreased by fed of isoflavone and/or grape seed oils. However, crude lipid and total cholesterol contents in liver were not affect of isoflavone and/or grape seed oil. The hepatic glutathione contents were increased by isoflavone and/or grape seed oil fed. The hepatic glutathione-S-transferase activity in isoflavone and/or grape seed oil supplemented groups were higher than that O group. Bone (scapular and femur bone) dry weight, femur of max weight and bending strength were no significant difference among groups. Our finding suggest that isoflavone/grape seed oils might have potential role for serum lipid profiles improvement and bone strength in vivo.

• Nutrition Research and Practice
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• 제1권3호
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• pp.206-211
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• 2007

This study estimated the isoflavone intake level in Koreans using Food Frequency Questionnaire and analyzed related variables. The results showed that the average daily intake of isoflavone in adults was shown as 23.1 mg. The isoflavone intake level at 50 percentile was 16.9 mg ($0{\sim}90\;mg$), and 10% of adults took almost 50 mg of isoflavone a day and 10% took about 5 mg a day. The major food sources for isoflavone in Koreans were in the order of soybean, soybean paste, soy milk, soybean curd (tofu), and bean sprouts; the intake was different depending on age, educational background, occupation, economic standard, and family type. The result showed higher isoflavone intake levels in the group over 30 years old and the highest isoflavone intake in subjects working in farming/fishery, followed by housemakers. According to the differences by families the families with elderly members showed 50% higher isoflavone intake than young families with friends or siblings. Depending on related ecological variables, therefore, various nutrition education programs should be developed for a variety of intakes of soybean foods, along with easy and simple cooking methods as parts of continuous research.