• 전력전자학회:학술대회논문집
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• 전력전자학회 2007년도 하계학술대회 논문집
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• pp.350-352
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• 2007

Modularized charge equalization converter for HEV lithium-ion battery cells is proposed in this paper, in which intra-module and inter-module charge equalization can be achieved at the same time. For intra-module charge equalization, the conventional flyback DC/DC converters of low power and small size are employed, in which all of the primary sides are coupled in parallel for selective charge of the specific under charged cell within the module. For inter-module charge equalization, the flyback DC/DC converters are also added, in which all the secondary windings are electrically linked in parallel for automatic charge balancing among the modules. An engineering sample of forty cells hiring the proposed cell balancing scheme is implemented and its experimental result shows that the proposed modularized charge equalization circuit has good cell balancing performance.

• Genomics & Informatics
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• 제21권2호
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• pp.19.1-19.13
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• 2023

Liver cancer, particularly hepatocellular carcinoma (HCC), poses a significant global threat to human lives. To advance the development of innovative diagnostic and treatment approaches, it is essential to examine the hidden features of HCC, particularly its 3D genome architecture, which is not well understood. In this study, we investigated the 3D genome organization of four HCC cell lines-Hep3B, Huh1, Huh7, and SNU449-using in situ Hi-C and assay for transposase-accessible chromatin sequencing. Our findings revealed that HCC cell lines had more long-range interactions, both intra-and interchromosomal, compared to human mammary epithelial cells (HMECs). Unexpectedly, HCC cell lines displayed cell line-specific compartmental modifications at the megabase (Mb) scale, which could potentially be leveraged in determining HCC subtypes. At the sub-Mb scale, we observed decreases in intra-TAD (topologically associated domain) interactions and chromatin loops in HCC cell lines compared to HMECs. Lastly, we discovered a correlation between gene expression and the 3D chromatin architecture of SLC8A1, which encodes a sodium-calcium antiporter whose modulation is known to induce apoptosis by comparison between HCC cell lines and HMECs. Our findings suggest that HCC cell lines have a distinct 3D genome organization that is different from those of normal and other cancer cells based on the analysis of compartments, TADs, and chromatin loops. Overall, we take this as evidence that genome organization plays a crucial role in cancer phenotype determination. Further exploration of epigenetics in HCC will help us to better understand specific gene regulation mechanisms and uncover novel targets for cancer treatment.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.59-63
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• 2004

By using PAGE, a study was made on the nonspecific esterase spectra of female genital organs and eggs in Bombyx mori L. The expression of 11 esterase bands was detected during ontogenesis of races and inter-races hybrids kept in Bulgaria. The gene activity of 9 esterase loci was assumed. Esterases specific for the spectrum of diapausing eggs were observed. In two esterase zones, intra- and inter-breed polymorphism was found. Based on the same breed specific expression, the existence of correspondence between esterase bands from spectra of different silkworm tissues and organs was suggested. Stage-specific expression of esterases in female genital glands, indicative of differentiated gene activity during ontogenesis, was established.

• Journal of Microbiology and Biotechnology
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• 제34권9호
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• pp.1848-1856
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• 2024

Chlorella sorokiniana green microalga offers many environmentally friendly applications, including wastewater treatment, biofertilizers, animal feed, and biofuel production. Different strains of C. sorokiniana have unique properties that may suit one application but not another. There is a need to distinguish between the many available strains of C. sorokiniana to choose the one that best fits the application. Consequently, our research goal was to develop strain-specific simple sequence repeat (SSR) markers to differentiate between the different strains. Seventeen markers spanning ten out of the twelve chromosomes of the C. sorokiniana genome were developed and validated on eight different strains from culture collections and our lab, and were then analyzed by fragment analysis. The results demonstrate the potential of these polymorphic markers to detect the genetic differences between the strains of C. sorokiniana, and to serve as useful tools for the intra-species population genetic analysis and conservation genetics studies of C. sorokiniana.

• 방송공학회논문지
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• 제13권2호
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• pp.200-213
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• 2008

H.264|MPEG-4 AVC는 ITU-T와 ISO/IEC 공동으로 결성된 JVT (Joint Video Team)에 의해서 정의된 가장 최신의 영상 압축 표준이다. H.264|MPE6-4 AVC는 효율적 부호화를 위하여 여러 방법이 제안되었는데, 화면 간 프레임(P-frame)에서의 화면 내 예측(Intra Prediction)의 경우 매크로블록마다 후보 모드 결정 및 율-왜곡 비용 계산에 따른 부호화 시간의 급격한 증가를 초래하여 고속화 방법의 필요성이 대두되고 있다 본 논문에서는 $16{\times}16$과 $4{\times}4$ 화면 내 예측 부호화 결과를 바탕으로, 두 예측 결과의 통계적 상관관계를 규정한 후, 이를 활용한 $4{\times}4$ 화면 내 예측의 후보 모드 수를 감소시키는 방법을 제안한다. 구체적으로는 화면 간 예측(Inter Prediction) 단계에서 결정된 움직임 벡터 정보를 이용하여 현재 매크로블록의 화면 내 예측이 필요한지를 미리 판정한 후, 매 화면 내 프레임(I-frame)의 $16{\times}16$ 화면 내 예측의 최종 후보 모드에 따른 $4{\times}4$ 화면 내 예측의 최종 결정 모드들의 발생분포를 누적 확률 순으로 배열하여 특정 누적 확률에 도달하기까지 만의 후보 모드들만을 예측에 포함하는 참조 테이블을 부호화 과정 중에 생성한 후 동일 GOP 내에 위치하는 모든 화면 간 프레임의 화면 내 예측 시 활용하게 된다. 제안하는 방법은 H.264|MPEG-4 AVC의 참조 소프트웨어인 JM11.0을 사용하여 실험하였으며, 총 부호화 시간을 최대 51.24% 감소시킬 수 있었으며 PSNR 감소와 비트율 증가는 무시할 정도의 작은 변화만 있었다.

• 통상정보연구
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• 제18권4호
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• pp.311-332
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• 2016

본 연구는 KS인증업체를 중심으로 KS인증시스템이 중소기업 수출성과에 미치는 영향 요인을 분석하여 국제환경에 중소기업의 수출경쟁력을 강화하는데 시사점을 제시하고자 연구를 수행하였다. 이를 검증하기 위하여 환경특성요인과 기업특성요인에 대한 선행연구를 통해 이 요인들이 KS인증시스템 표준화 요인에 영향을 미치는 것을 규명하고 KS인증시스템 표준화 요인이 수출성과에 영향을 미치는 요인을 분석하였다. 분석을 위해 SPSS 21.0을 사용하여 빈도분석, 타당성분석, 신뢰성분석, 다중회귀분석을 실시하였다. 분석결과 기업특성요인의 경영자마인드, 종업원표준인식, KS인증교육이 KS인증시스템 표준화 요인에 영향을 미치는 것으로 나타났다. 그리고 KS인증시스템 표준화 요인이 수출성과에 영향을 미치는 것으로 나타났다. 실증분석한 결과에 따라 중소기업에 다음과 같은 시사점을 제시할 수 있다. 첫째, 수출성과를 강화하기 위해서는 중소기업 최고경영자는 KS인증시스템에 대한 사내표준화 마인드를 고양시키는 지원이 요구된다. 둘째, 중소기업 종업원에 대한 표준화 인식이 높아질 수 있는 KS 인증 교육이 필요하다. 셋째, 제품품질 향상을 위해 ISO 인증, FTA관련 업무 사내 표준화 시스템 활용이 필요하다.

• IMMUNE NETWORK
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• 제23권2호
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• pp.17.1-17.16
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• 2023

Latent membrane protein 2A (LMP2A), a latent Ag commonly expressed in Epstein-Barr virus (EBV)-infected host cells, is a target for adoptive T cell therapy in EBV-associated malignancies. To define whether individual human leukocyte antigen (HLA) allotypes are used preferentially in EBV-specific T lymphocyte responses, LMP2A-specific CD8⁺ and CD4⁺ T cell responses in 50 healthy donors were analyzed by ELISPOT assay using artificial Ag-presenting cells expressing a single allotype. CD8⁺ T cell responses were significantly higher than CD4⁺ T cell responses. CD8⁺ T cell responses were ranked from highest to lowest in the order HLA-A, HLA-B, and HLA-C loci, and CD4⁺ T cell responses were ranked in the order HLA-DR, HLA-DP, and HLA-DQ loci. Among the 32 HLA class I and 56 HLA class II allotypes, 6 HLA-A, 7 HLA-B, 5 HLA-C, 10 HLA-DR, 2 HLA-DQ, and 2 HLA-DP allotypes showed T cell responses higher than 50 spot-forming cells (SFCs)/5×105 CD8⁺ or CD4⁺ T cells. Twenty-nine donors (58%) showed a high T cell response to at least one allotype of HLA class I or class II, and 4 donors (8%) had a high response to both HLA class I and class II allotypes. Interestingly, we observed an inverse correlation between the proportion of LMP2A-specific T cell responses and the frequency of HLA class I and II allotypes. These data demonstrate the allele dominance of LMP2A-specific T cell responses among HLA allotypes and their intra-individual dominance in response to only a few allotypes in an individual, which may provide useful information for genetic, pathogenic, and immunotherapeutic approaches to EBV-associated diseases.

• 한방안이비인후피부과학회지
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• 제21권3호
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• pp.20-35
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• 2008

Objective : This study was designed to investigate the effects of Curcuma longa Rhizoma(CLR) on asthma. Methods : Detecting splenocyte proliferation rates, cytokines and antigen specific antibody isotypes in bronchoalveolar lavage fluid (BALF). In addition, the present author I also investigated changes in spleen and histopathological changes of lung tissues. Results : Oral administration of CLR lowered spleen weight and splenocyte proliferation rates. In addition, levels of IL-4, IL-17A and Granulocyte/Macrophage Colony-Stimulating Factor(GM-CSF), Th2 driven cytokines, were lowered respectively and IFN-g, and Th1 driven cytokine, were elevated by CLR. Levels of Ovalbumin(OVA) specific IgE and IgGl in BALF were also lowered by oral administration of CLR too. Conclusion : CLR is useful to treat patients with asthma and the mechanisms are related to the in suppression of Th2 skewing reactions.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.42-42
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• 2014

Mating of tetrapolar mushrooms is regulated by to chromosomal loci, A and B. A locus contains A gene that expresses a homeodomain protein whereas B locus contains multiple pheromones and receptor genes. In order to characterize the mating loci in Korean cultivated strains of Lentinula edodes, one hundred monokaryotic myclelia were isolated from the basidiospores of cultivated strains, including Cham-A-Ram, Sanjo701, and Sanjo707. Both mating loci were amplified using primer sets targeting conserved sequence regions for homeodomain (HD), pheromone, and receptor genes. Subsequent sequence analysis revealed that the Korean strains contained significant variations in the homeodomain of A locus, even within the same A1 or A2 mating type. Similarly, B locus was also highly diversified in the sequences of pheromones and receptors as well as gene organization. These results enabled us to design mating type-specific probes which can distinguish mating type of each strain. The specificity was confirmed by between intra- and inter-strain mating experiment.

• 대한수의학회지
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• 제7권2호
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• pp.46-50
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• 1967

The pathologic studies on the cottonseed meal poisoning in chicken were performed by feeding a diet containing 50 per cent cottonseed meal. The results obtained are summarized as follows: The specific effect of cottonseed meal on chicken was apparently limited to the parenchymatous tissue and blood vessels, where its major pathologic manifestations were degenerative changes in acute cases fed for a period of 7 days, while necrosis of portal and central veins of liver and consequent perivascular hemorrhage and coagulative necrosis of liver and intra-glomerular hemorrhage were characteristic lesions in chronic cases fed for a period 20, 40 and 60 days. In addition, specific cottonseed pigment cells were observed in small number in villus of small intestine in acute cases and in large number in villus, liver and spleen in chronic cases.