Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Developing Strain-Specific Simple Sequence Repeat (SSR) Markers for Chlorella sorokiniana

  • Mais Sweiss (Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa Applied University) ;
  • Maen Hasan (Department of Plant Production and Protection, Faculty of Agricultural Technology, Al-Balqa Applied University) ;
  • Nidal Odat (Department Medical Laboratory Sciences, Faculty of Science, Al-Balqa Applied University)
  • Received : 2024.04.16
  • Accepted : 2024.07.05
  • Published : 2024.09.28
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Abstract

Chlorella sorokiniana green microalga offers many environmentally friendly applications, including wastewater treatment, biofertilizers, animal feed, and biofuel production. Different strains of C. sorokiniana have unique properties that may suit one application but not another. There is a need to distinguish between the many available strains of C. sorokiniana to choose the one that best fits the application. Consequently, our research goal was to develop strain-specific simple sequence repeat (SSR) markers to differentiate between the different strains. Seventeen markers spanning ten out of the twelve chromosomes of the C. sorokiniana genome were developed and validated on eight different strains from culture collections and our lab, and were then analyzed by fragment analysis. The results demonstrate the potential of these polymorphic markers to detect the genetic differences between the strains of C. sorokiniana, and to serve as useful tools for the intra-species population genetic analysis and conservation genetics studies of C. sorokiniana.

Keywords

Acknowledgement

This research was funded by the Dean of Scientific Research and Innovation at Al-Balqa Applied University in Jordan.

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