• Title/Summary/Keyword: intestinal microorganism

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Effect of Feeding Live Yeast (Saecharomyces cerevisiae) on Nutrient Utilization and Changes of Intestinal Microorganism in Broiler Chicks (육계에 있어서 활성효모 (Saccharomyces cerevisiae)의 급여가 영양소 이용성과 장내 미생물의 변화에 미치는 영향)

  • 이현우;김인호;김춘수
    • Korean Journal of Poultry Science
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    • v.22 no.4
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    • pp.203-211
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    • 1995
  • In order to investigate the effect of feeding live yeast on growth performance, nutrients utilization, tibia mineral deposit and intestinal microorganism changes, a growth assay was conducted with 360 broiler chicks. Treatments were four levels of yeast as 0, 0.025, 0.05 and 0.1% in 1.83% tricalcium phosphate and two levels of yeast as 0 and 0.05% in 1.15% tricalcium phosphate. The crude protein content of live yeast was 45%, and 97% of it was in the pure protein form, with 46.6% of essential amino acids and 53.4% of non-essential amino acids. Growth performance was tended to increase by feeding the yeast but there was no significant difference(P>.05). The protein digestibility was increased as the feeding level of yeast increased. However, digestibilities of fat, fiber, calcium and phosphorus were not affected by the yeast. Ash and calcium content of tibia were increased as the level of yeast increased. Total number of E. coli in small intestine was significantly decreased(P<.05) in chicks fed yeast. Total number of Lactobaci1lus was significantly increased by the yeast feeding. The changes of microorganism in cecum had the same trend with the changes of microorganism in small intestine.

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Enzymological Evaluation of Bowel Inflammation Inhibitory Activity and Intestinal Microbial Flora Improvement by Enzymatic Hydrolysate of Schizandrae Fructus (오미자 효소가수분해물에 의한 장내 세균총 개선 및 장관 염증 억제 효과의 효소학적 평가)

  • Ryu, Il-Hwan;Kwon, Tae-Oh;Lee, Kang-Soo;Yun, Yong-Gab
    • Korean Journal of Pharmacognosy
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    • v.38 no.4
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    • pp.363-371
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    • 2007
  • Schizandrae Fruits has been used as a traditional Oriental medicine for treatment of many stress-induced diseases. In the present study, we investigated inhibitory activity of enzymatic hydrolysate of Schizandrae fructus (SC-EX) in growth of tested intestinal microorganism and activity of bowel inflammation related enzyme. SC-EX was added to the proteose peptone-yeast extract-fildes (PYF) media to investigation the effect on the growth of type culture of intestinal microorganism. The growth of lactic acid bacteria such as Bifidobacterium species and Lactobacillus species was accelerated by more than 3% concentration of SC-EX. But, growth of harmfulness bacteria such as E.coli, Clostridium sp. Staphylococcus sp. Streptococcus sp. was inhibited by more than 3% concentration of SC-EX. Also, SC-EX was exhibited a concentration-dependent inhibitory activity of the bowel inflammation related enzymes. The SC-EX was showed 76% and 92% inhibitory activity of 5-lipoxygenase and cyclooygenase at 5% additional concentration respectively. Our results indicated that SC-EX may possess improvement effect on the intestinal flora and Anti-inflammatory effect on the bowel.

Symbiotic relationship between termite, Reticulitermes Speratus kyushuenesis Morimoto, and its intestinal microorganisms (흰개미 (Reticulitermes Speratus kyushuenesis Morimoto)와 장내 미생물과의 공생관계)

  • 박현철
    • The Korean Journal of Soil Zoology
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    • v.3 no.1
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    • pp.19-24
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    • 1998
  • The importance of the gut microorganism in the termite, Reticulitermes Speratus kyushuenesis Morimoto, was estimated by feeding with several antibiotics. Antibiotics which killed the bacterium, but not the fungi (Ampicillin, Kanamycin, Erythromycin), or killed both the microorganismw (Cephaloridine, Tetracycline) reduced the life span of the termite from 245 days to about 15 days. On the other hand, some antibiotics, penicillin and pimaricin, did not greatly reduce the life span of the termite.

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Effect of Wormwood Ethanol Extract on Human Intestinal Microorganisms. (쑥 추출물이 인체 장내 미생물에 미치는 영향)

  • Kwon, Dong-Jin;Park, Jong-Hyun;Kwon, Min;Yoo, Jin-Young;Koo, Young-Jo
    • Microbiology and Biotechnology Letters
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    • v.27 no.2
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    • pp.102-106
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    • 1999
  • To investigate the effect of wormwood extract on intestinal Microorganisms of human, we examined the changes of the intestinal microflora of 6 volunteers for 13 days. Numbers of total anaerobes in feces of 6 volunteers showed 1010cfu/g before and after intake of wormwood extract. In case of beneficial Microorganisms, wormwood extract did not have an effect on the growth of bifidobacteria and Lactobacillus spp. On the other hand, the growth of C. perfringens and E. coli, the harmful Microorganisms of human intestines, was inhibited by the wormwood extract.

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Culture-Based and Denaturing Gradient Gel Electrophoresis Analysis of the Bacterial Community Structure from the Intestinal Tracts of Earthworms (Eisenia fetida)

  • Hong, Sung-Wook;Kim, In-Su;Lee, Ju-Sam;Chung, Kun-Sub
    • Journal of Microbiology and Biotechnology
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    • v.21 no.9
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    • pp.885-892
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    • 2011
  • The bacterial communities in the intestinal tracts of earthworm were investigated by culture-dependent and -independent approaches. In total, 72 and 55 pure cultures were isolated from the intestinal tracts of earthworms under aerobic and anaerobic conditions, respectively. Aerobic bacteria were classified as Aeromonas (40%), Bacillus (37%), Photobacterium (10%), Pseudomonas (7%), and Shewanella (6%). Anaerobic bacteria were classified as Aeromonas (52%), Bacillus (27%), Shewanella (12%), Paenibacillus (5%), Clostridium (2%), and Cellulosimicrobium (2%). The dominant microorganisms were Aeromonas and Bacillus species under both aerobic and anaerobic conditions. In all, 39 DNA fragments were identified by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis. Aeromonas sp. was the dominant microorganism in feeds, intestinal tracts, and casts of earthworms. The DGGE band intensity of Aeromonas from feeds, intestinal tracts, and casts of earthworms was 12.8%, 14.7%, and 15.1%, respectively. The other strains identified were Bacillus, Clostridium, Enterobacter, Photobacterium, Pseudomonas, Shewanella, Streptomyces, uncultured Chloroflexi bacterium, and uncultured bacterium. These results suggest that PCR-DGGE analysis was more efficient than the culturedependent approach for the investigation of bacterial diversity and the identification of unculturable microorganisms.

Effect of Feeding Yeast (Saceharomyces eerevisiae) on Growth Performance and Changes of Intestinal E. coli in Broiler Chicks (효모의 급여가 육계의 성장 및 장내 대장균의 변화에 미치는 영향)

  • 이현우;김인호;김춘수;손중천
    • Korean Journal of Poultry Science
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    • v.24 no.2
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    • pp.67-72
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    • 1997
  • In order to investigate the effect of feeding live yeast (Sacckaromyces cerevisiae) on the growth performance and changes of intestinal microorganism (E. coli), a growth assay was conducted with 144 broiler chicks. Treatments were consisted of corn-soybean meal control, 0.05% live yeast, and 0.05% dead yeast. Most of the chick protein of the live yeast was in the pure protein form, and had a high amino acid composition with 47% of essential amino acids and 53% of non-essential amino acids. No differences in growth performance were shown among dietany treatments. Total number of E. coli in the small intestine of chicks fed either live or dead yeast was significantly reduced compared to chicks fed the control diet. Although the changes of E. coli in the cecum were not identical to differences in the small intestine, the changes of E. coli in the cecurn had a similar trend.

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Development of Intestinal Microorganisms and Molecular Ecological Methods for Analysis of Intestinal Ecosystem in the Neonate (신생아 장내 미생물의 형성과 이의 분석을 위한 분자 생태학적 기술)

  • Park Ja Ryeong;Bae Jin-Woo;Rhee Sung-Keun;Nam Young-Do;Oh Jong-Won;Park Yong-Ha
    • Microbiology and Biotechnology Letters
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    • v.33 no.3
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    • pp.159-168
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    • 2005
  • Up to date, a number of review papers were reported on intestinal microorganisms that influence the health and disease of human being and diet that directly influence the establishment of intestinal microbial populations. Importance of studying intestinal microorganisms in the neonate arises from the easy approach to studying initial acquisition and settlement of intestinal microorganisms. Despite of the importance, few studies of neonatal intestinal microorganisms have been carried out and there is no paper focusing the factors to influence the development of intestinal microorganisms and molecular ecological methods for the analysis of intestinal ecosystem in the neonate. In this review, we summarized the status of our current knowledge of basic initial acquisition and settlement of intestinal microorganisms. And recent development of molecular ecological methods in studying the intestinal microbiology was also discussed.

The Impact of Gut Microbiota in Human Health and Diseases: Implication for Therapeutic Potential

  • Ha, Eun-Mi
    • Biomolecules & Therapeutics
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    • v.19 no.2
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    • pp.155-173
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    • 2011
  • Humans have and hold 100 trillion intestinal bacteria that are essential for health. For millions of years human-microorganisms interaction has co-evolved, and maintained close symbiotic relationship. Gut bacteria contributes to human health and metabolism, and humans provides the optimum nutrition-rich environment for bacteria. What is the mechanism of the host distinguishing the intestinal bacteria as its cohabiting partner and what kind of benefits does the gut microbiota provide the human are the fundamental questions to be asked and solved in order to make human life a higher quality. This review explains the physiological relationship and mutualism between the host and gut microorganism, and highlights the potential therapeutic approach for treating diseases, maintaining and improving health based on these correlations.

The Nutritional Composition of Bamboo Shoots and the Effects of its Fiber on Intestinal Microorganisms (죽순의 영양성분 및 죽순의 식이섬유가 장내미생물에 미치는 영향)

  • Park, Eun-Jin;Jhon, Deok-Young
    • Journal of the Korean Society of Food Culture
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    • v.28 no.5
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    • pp.502-511
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    • 2013
  • This study evaluated the composition two popular species of edible bamboo shoots in Korea (Phyllostachyspubescens and Sinoarundinarianigra) and the effect of their abundant dietary fiber on intestinal microorganisms in healthy young women. The ranges of total moisture, crude protein, crude lipid, crude ash, and dietary fiber content were 87.190.8, 2.943.5, 0.150.39, 0.411.05, and 4.206.15% (wet weight basis), respectively. Moisture and crude ash content increased after heat treatment; however, crude protein, crude lipid, and dietary fiber content were reduced after heating. The major minerals found in bamboo shoots were potassium, phosphorous, sulfur, magnesium, and calcium. In addition, glucose and fructose were abundant free sugars, while asparagine and tyrosine were the most abundant free amino acids. Approximately 70% of the total free fatty acids found in bamboo shoots were linoleic acid and linolenic acid. The ascorbic acid content was 6.60~17.56 mg/100 g (wet weight basis), and one phenolic compound, p-hydroxy benzoic acid, was 0.10.2% (wet weight basis) and detected by HPLC analysis. The intake of bamboo shoots for seven days significantly increased viable cell counts of Lactobacillus spp. and reduced viable cell counts of Bacteriodes spp. in feces (p<0.05). In our data, bamboo shoots may be useful in the food industry as high dietary fiber ingredients.