• Advances in Computational Design
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• 제8권3호
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• pp.237-253
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• 2023

Torque ripple content and variable switching frequency operation of conventional direct torque control (DTC) are reduced by the integration of space vector modulation (SVM) into DTC. Integration of space vector modulation to conventional direct torque control known as SVM-DTC. It had been more frequently used method in renewable energy and machine drive systems. In this paper, SVM-DTC is used to control the rotor side converter (RSC) of a wind driven doubly-fed induction generator (DFIG) because of its advantages such as reduction of torque ripples and constant switching frequency operation. However, flux and torque ripples are still dominant due to distorted current waveforms at different operations of the wind turbine. Therefore, to smoothen the torque profile a Neural Network Controller (NNC) based SVM-DTC has been proposed by replacing the PI controller in the speed control loop of the wind turbine controller. Also, stability analysis and simulation study of DFIG using process reaction curve method (RRCM) are presented. Validation of simulation study in MATLAB/SIMULINK environment of proposed wind driven DFIG system has been performed by laboratory developed prototype model. The proposed NNC based SVM-DTC yields superior torque response and ripple reduction compared to other methods.

• Journal of Microbiology and Biotechnology
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• 제18권8호
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• pp.1357-1363
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• 2008

The $P_{entC}$ promoter of the entCERA operon encoding enzymes for enterobactin biosynthesis in Escherichia coli is tightly regulated by the availability of iron in the culture medium. In iron-rich conditions, the $P_{entC}$ promoter activity is strongly repressed by the global transcription regulator Fur (ferric uptake regulator), which complexes with ferrous ions and binds to the Fur box 19-bp inverted repeat. In this study, we have constructed the expression vector pOS2 containing the $P_{entC}$ promoter and characterized its repression, induction, and modulation by quantifying the expression of the lacZ reporter gene encoding $\beta$-galactosidase. $\beta$-Galactosidase activities of E. coli transformants harboring pOS2-lacZ were highly induced in the presence of divalent metal ion chelators such as 2,2'-dipyridyl and EDTA, and were strongly repressed in the presence of excess iron. It was also shown that the basal level $\beta$-galactosidase expression by the $P_{entC}$ promoter was drastically decreased by incorporating the fur gene into the expression vector. Since the newly developed iron chelator-inducible expression system is efficient and cost-effective, it has wide applications in recombinant protein production.

• 한국양식학회지
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• 제11권4호
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• pp.457-463
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• 1998

미꾸라지(Misgrunus mizolepis)의 DNA로부터 클론된 핵기질 부착부위(MAR)를 포함하는 발현벡터인 pUC19N6-luc 벡터를 구성하였다. 이를 물고기 CHSE-214 세포주에 electroporation으로 transfection 시킨 후 유전자의 발현율, 벡터의 copy 수 및 염색체내 삽입 양상을 luciferase 활성도 분석, PCR 및 Southern blotting를 통해 분석하였다. 대조군 발현벡터에 luciferase 유전자는 전형적인 transient 발현양상을 나타내는데 비해, 미꾸라지 MAR가 포함된 pUC19N6-luc 벡터의 luciferase 유전자의 발현은 transfection 후 5일째부터 급격히 증가하는 양상을 보였다. Transfection된 CHSE-214 세포내에서 pUC19N6-luc 벡터는 대조군 벡터에 비해 높은 copy 수를 유지하였으나, 염색체내 삽입은 거의 비슷한 시간에 일어났다. 결론적으로 transfection 후 시간경과에 따른 pUC19N6-luc 벡터내의 luciferase 유전자의 발현 증가에 미치는 MAR의 효과는 벡터 copy수 증가 때문이 아니라, 염색체내 삽입후 형성되는 전사활성구조의 형성에 기인하는 것으로 판단된다.

• 미생물학회지
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• 제29권1호
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• pp.16-24
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• 1991

The yeast gene THR1 encodes the homoserine kinase (EC 2.7.1.39: HKase) which catalyses the first step of the threonine specific arm at the end of the common pathway for methionine and threonine biosynthesis. A recombinant plasmid pMC3 (12.6 kilobase pairs, vector YCp50) has been cloned into E. coli HB101 from a yeast genomic library through its complementing activity of a thr1 mutation in a yeast recipient strain M39-1D. When subcloned into pMC32 (8.6kbp, vector YRp7) and pMC35 (8.3 kbp, vector YIp5), the HindIII fragment (2.7 kbp) of pMC3 insery was positive in the thrI complementing activity in both yeast and E. coli auxotrophic strains. The linearized pMC35 was introduced into the original recipient yeast strain and the mitotically stable chromosomal integrant was identified among the transformants. Through the tetrad analysis, the integration site of the pMC35 was localized to the region of THR1 structural gene at an expected genetic distance of approximately 11.1 cM from the ARG4 locus on the right arm of the yeast chromosome VIII. When episomically introduced into the auxotrophic cells and cultured in Thr omission liquid medium, the cloned gene overexpressed the HKase in the order of thirteen to fifteenfold, as compared with a wildtype. HKase levels are repressed by addition of threonine at the amount of 300 mg/l and 1, 190 mg/l for pMC32 and pMC3, respectively. Data from genetic analysis and HKase response thus support that the cloned HindIII yeast DNA fragment contains the yeast thr1 structural gene, along with necessary regulatory components for control of its proper expression.

• Food Science and Biotechnology
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• 제17권6호
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• pp.1221-1227
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• 2008

The application of the cellulase gene (celA) as a selection marker of food-grade integration system was investigated in Lactobacillus (Lb.) casei, Lactococcus lactis, and Leuconostoc (Leu.) mesenteroides. The 6.0-kb vector pOC13 containing celA from Clostridium thermocellum with an integrase gene and a phage attachment site originating from bacteriophage A2 was used for site-specific recombination into chromosomal DNA of lactic acid bacteria (LAB). pOC13 was also equipped with a broad host range plus replication origin from the lactococcal plasmid pWV01, and a controllable promoter of nisA ($P_{nisA}$) for the production of foreign proteins. pOC13 was integrated successfully into Lb. casei EM116, and pOC13 integrants were easily detectable by the formation of halo zone on plates containing cellulose. Recombinant Lb. casei EM 116::pOC13 maintained these traits in the absence of selection pressure during 100 generations. pOC13 was integrated into the chromosome of L. lactis and Leu. mesenteroides, and celA acted as an efficient selection marker. These results show that celA can be used as a food-grade selection marker, and that the new integrative vector could be used for the production of foreign proteins in LAB.

• Agribusiness and Information Management
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• 제12권2호
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• pp.40-54
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• 2021

Market integration and prices in pulse crops like black gram play an important role in determining the production decisions of the farmers and diversification towards high value nutritious crops. In this context, the present study explores extent of market integration and price transmission in selected major black gram markets in Andhra Pradesh using Johansen co-integration, Vector Error Correction Model and Granger causality test. The study used monthly prices data of black gram (Rs/quintal) sourced from selected markets of Srikakulam, Krishna and Kurnool spanning January, 1990 to December, 2019. The results of the study strongly buttressed the existence of co-integration and interdependence of selected black gram markets in Andhra Pradesh. However, the speed of adjustment of the prices found to be moderate in Krishna market and quite weaker in Srikakulam market and thereby prices correct a small percentage of the disequilibrium in these markets with the greatest percentage by the external and internal forces. So, it necessitates the need for future research, to investigate the influence of external and internal factors such as market infrastructure, Government policy and self-sufficient production, product characteristics and utilization towards market integration. As there exists only unidirectional causality from Krishna to Kurnool and from Krishna to Srikakulam markets, it calls for strengthening the information technology for flow of market information regularly to help the farmers for increasing their income.

• Structural Engineering and Mechanics
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• 제28권5호
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• pp.549-570
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• 2008

Numerical integration is an efficient approach for nonlinear dynamic analysis. In this paper, general category of the implicit integration errors will be discussed. In order to decrease the errors, Dynamic Relaxation method with modified time step (MFT) will be used. This procedure leads to an alternative algorithm which is very general and can be utilized with any implicit integration scheme. For numerical verification of the proposed technique, some single and multi degrees of freedom nonlinear dynamic systems will be analyzed. Moreover, results are compared with both exact and other available solutions. Suitable accuracy, high efficiency, simplicity, vector operations and automatic procedures are the main merits of the new algorithm in solving nonlinear dynamic problems.

• Journal of applied mathematics & informatics
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• 제5권3호
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• pp.735-748
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• 1998

We analyze the error in the p version of the of the finite element method when the effect of the quadrature error is taken in the load vector. We briefly study some results on the $H^{1}$ norm error and present some new results for the error in the $L^{2}$ norm. We inves-tigate the quadrature error due to the numerical integration of the right hand side We present theoretical and computational examples showing the sharpness of our results.

• 전력전자학회:학술대회논문집
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• 전력전자학회 1998년도 전력전자학술대회 논문집
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• pp.217-220
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• 1998

In this paper, for high performance as drive, in the speed sensorless vector control of induction motor, introduced flux estimator of voltage model and error compensation algorithm using closed loop integration method, and then we proposed a improved flux estimation method of high accuracy. And the rotor speed is estimating using the stator current and the estimated flux, it is used speed information. The proposed scheme is verified through digital simulations and experiments for 3.7[kW] induction motor and shows good dynamic performance.

• 전자공학회논문지B
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• 제31B권12호
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• pp.100-107
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• 1994

This article describes the hardware design scheme of Hamming MAXNET algorithm which is appropriate for binary pattern classification with minimum HD measurement between stimulus vector and storage vector. Circuit integration is profitable to Hamming MAXNET because the structure of hamming network have a few connection nodes over the similar neuro-algorithms. Designed hardware is the two-layered structure composed of hamming network and MAXNET which enable the characteristics of low power consumption and fast operation with biline volgate sensing scheme. Proposed Hamming MAXNET hardware was designed as quantize-level converter for simulation, resulting in the expected binary pattern convergence property.