• Journal of The Korean Association of Information Education
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• v.14 no.2
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• pp.209-218
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• 2010

Robot Curriculum based education in Insects Robot help elementary school students better understand how a robot works. This robot curriculum is aimed at elementary school students in fifth grade. This study progressed with LEGO(R) MINDSTORMS(R) NXT, departed 6 groups, reached the insect's movement, designed robot like insects. This curriculum enhanced discussion prowess and improved the ability of building robot. During this study, most of the students were attracted to the action of the robot-like insect's movement.

• Research in Plant Disease
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• v.19 no.2
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• pp.114-117
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• 2013

To facilitate their spread, plant viruses have developed several methods for dispersal including insect and seed transmission. While insect transmission requires virus stability against insect digestion, seed-transmitted viruses have to overcome barriers to entry into embryos. Bean pod mottle virus (BPMV) is transmitted through seed at levels typically below 0.1%, but co-infection with Soybean mosaic virus (SMV) enhanced the seed transmission rate of BPMV in one experiment. In contrast, the rate of SMV seed transmission was not affected by BPMV co-infection. In a second preliminary study, the rate of SMV transmission was lower in an isoline of Williams 82 that contained a null mutation for the Kunitz trypsin inhibitor gene than in Williams 82. In this preliminary study, we observed that factors such as protease inhibitor expression and dual infection may affect the frequency of seed transmission of BPMV and SMV.

• The Korean Journal of Pesticide Science
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• v.3 no.1
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• pp.78-83
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• 1999

Sublethal effects of imidacloprid (30 and 100 ppm) and insect growth regulators; flufenoxuron (3 and 10 ppm), triflumuron (10 ppm), and teflubenzuron (3 ppm), were tested by treatment via larval rearing medium of a housefly, Musca domestica, in laboratory. Pupal weight was significantly reduced by treatment to the 3rd larvae with high concentrations of imidacloprid (100 ppm) and flufenoxuron (10 ppm), and the adults that survived the flufenoxuron 10 ppm treatment deposited significantly fewer eggs compared with controls and other treatments. Adult longevity and egg viability, however, were not affected by any of the treatments.

• International Journal of Industrial Entomology and Biomaterials
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• v.28 no.1
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• pp.1-4
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• 2014

The effect of an artificial diet on developmental rate, a life history parameter, was examined for the swallowtail butterfly Papilio xuthus. Artificial insect diets are an essential component of many insect rearing systems that produce insects for research purposes. Complex agar-gelled diets are generally prepared in large batches and used shortly after preparation because the degradation of perishable diet ingredients, such as vitamins and fatty acids, can adversely affect insect quality (Brewer 1984). However, the timing of diet preparation may be inconvenient, and large batches wasteful, if the unused excess is discarded. The percentage of pupation varied considerably, with no significant differences among diets, on which a maximum pupation percentage of 83% was observed. Pellet-type diets were investigated with the aim of developing a more easily prepared diet. The extrusion of the artificial diet under high temperature and pressure may induce desirable chemical and physical changes in the extruded product. The purpose of the present study was to develop an artificial diet for rearing P. xuthus.

• Korean journal of applied entomology
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• v.59 no.4
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• pp.295-301
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• 2020

In this study, we aimed to investigate the changes in the catch rate of Monochamus saltuarius, based on sexual maturation by using aggregation-sex pheromone traps. Ovariole development of caught M. saltuarius females was compared to that of the ones not caught using traps. In a mesh cage set up at the Hongneung experimental forest, we placed a multi-funnel trap with or without an aggregation-sex pheromone lure. M. saltuarius adults, which emerged from pine logs, were grouped in four according to the emergence dates (0, 1, 7, and 10 days after emergence [DAE]). We released beetles into the mesh cage to investigate the catch rate using the traps. In each group, a total of 80 beetles (20 beetles × 4 replications) were tested, making it a total of 320 beetles. Among the four groups, M. saltuarius adults in the 7 DAE group were caught more frequently using the traps, especially with a pheromone lure; the other groups showed a low catch rate. A similar number of female and male beetles were caught using the traps. Regarding ovariole development, all the female adults in the 0 and 1 DAE groups were immature, while those in the other two groups were completely developed. Therefore, aggregation-sex pheromone traps might have a limitation in the prevention of pine wilt disease because of the transmission of pine wood nematode during maturation feeding of newly emerged M. saltuarius adults. However, aggregation-sex pheromone traps can be effective for collecting sexually mature M. saltuarius adults, for the investigation of seasonal occurrence of beetles in forests.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.2
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• pp.220-227
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• 2007

Sweetpotato fields in Korea are highly infected with virus and virus like diseases that greatly diminish both yield and quality as indicated by field observations and laboratory tests. In order to solve this problem, there is an urgent need to produce and mass propagate virus-free planting materials for distribution to the farmers. These experiments were conducted, firstly, to determine the most appropriate culture media, nutrient solution, and cutting intervals to maintain growth and vigor of tissue cultured plantleta as mother plants for propagation in insect-proof greenhouse. And as a labor saving method, the production efficiency of plug trays for rapid propagation of stem cuttings as a source of planting materials was likewise evaluated. Results showed that plants grown in medium B supplied with 0.5 and 1.0 strength of MS nutrients had high growth rate, and 20-day cutting interval was the best. 72-plug tray was better than 128-plug. Secondly, it was to develop a technique for the production of first-generation seed roots using hydroponics cultivation system. The yield of virus-free plants propagated in the non-insect proof and open-field cultivation was 2,402 kg/10a, 6% higher than those in the insect-proof cultivation, and the rate of virus re-infection was 18% higher compared to 3.3% with insect-proof cultivation. Lastly, it was to investigate the growth performance of virus free plants in farmers' field. Differences were existed in the yield depending on the variety used, but virus free plants showed an increase of $6{\sim}24%$ over virus infected plants.

• The Journal of the Korea Contents Association
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• v.14 no.8
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• pp.84-94
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• 2014

Among artworks of Shin Sa-Im-Dang, 'grass and insect painting' was drawn the rustic materials that cannot be seen without a loving attention because they are too small and insignificant creatures. Likewise, 'grass and insect painting' is work that can feel the wonder of life, simplicity, and womanly sensitivity unique. Therefore, beauty of Korea can be found through the works. It needs to develop high value-added culture products with her works which contained this unique beauty of Korea and artistic soul. In this study, I tried to develop the scarf textile designs which had Korean sentiment with the Shin Sa-Im-Dang's 'grass and insect painting' and to make the scarves with the textile designs developed. For the purpose, theoretical examination about her art world and artworks was first performed. And then six scarf textile designs which based on them were developed using adobe illustrator 10.0, computer design program. The textile designs developed were printed on 100% silk satin using textile digital printing system. Six scarves were made with them.

• Journal of Microbiology and Biotechnology
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• v.11 no.3
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• pp.482-490
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• 2001

The gene for glycoprotein B (gB2) of HSV-2-strain G was subcloned, sequenced, recombinated into the lacZ-HcNPV, expressed in insect cells, and compared with the homologous gene of other HSV-2 strains. The ORF of the gB2 gene was 2,715 bp. The overall nucleotide sequence homology of te gB2 gene compared ith that of the two previously reported HSV-2 strains appeared to be over 98%. A recombinant virus named Baculo-gB2 protein in insect cells. The recombination was confirmed by a PCR and the expression was demonstrated by radio immunoprecipitation. Insect cells infected with the Baculo-gB2 virus synthesized and processed gB2 with approximately 120 kDa in the cells, and then secreted it into the culture media, where it reacted with a nomoclonal antibody to gB2. The gB2 polypeptide contained two main hydrophobic regions (a signal sequence from 1 to 23 amino acid residues, and a membrane anchor sequence from aa 745 to 798), eight N-glycosylation sites evenly distributed, and was rich in alanine (11.2%). Antibodies to this recombinant protein that were raised in mice recognized the viral gB2 and neutralized the infectivity of the HSV-2 in vitro. There results show that the gB2 protein was successfully porduced in insect cells and could be used to raise a protective neutralizing antibody. Accordingly, this particular recombinant protein may be useful in the development of a subunit vaccine.

• The Korean Journal of Mycology
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• v.38 no.2
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• pp.99-104
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• 2010

Dead insects infected with fungi were collected from mountains and islands in several locations in Korea from 2003 to 2007. Morphological characteristics of the fungal fruiting bodies produced on the insect samples were examined. A total of 542 entomopathogenic fungal isolates were obtained from the insect samples and identified based on their morphological and cultural characteristics. Three species of Beauveria, eight species of Cordyceps and four species of Isaria were mainly isolated from the insect samples. The other entomopathogenic fungi isolated from the insect samples were Metarhizium anisopliae, Nomuraea rileyi, Paecilomyces sp. and Verticillium sp.

• Korean Journal of Agricultural Science
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• v.24 no.1
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• pp.21-28
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• 1997

For the investigation of occurrence of insect pests and their natural enemies, total number of 353 controlled agricultural farms were visited and surveyed. In the 73% of total controlled agricultural farms, 52 species insect pests were occurred. Among these pests, the western flower thrip (Frankliniella occidentalis), the two spotted spider mite (Tetranychus urticae), the greenhouse whitefly (Trialeurodes vaporariorum), the cotton aphid (Aphis gossypii) and the green peach aphid (Myzus persicae) were mainly occurred. On the other hand, small numbers of natural enemies were sustained in 40 controlled agricultural farms, which was 11% and 17% of visited farms and pest occurred farms, respectively. For the most part of collected natural enemies, there were included in Braconid, Coccinellid and Anthocorid, as 63, 20 and 8% of them, respectively.