• Title/Summary/Keyword: inner frame

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Molecular Characterization and Expression Analysis of Ribosomal Protein S6 Gene in the Cashmere Goat (Capra hircus)

  • Bao, Wenlei;Hao, Xiyan;Zheng, Xu;Liang, Yan;Chen, Yuhao;Wang, Yanfeng;Wang, Zhigang
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.11
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    • pp.1644-1650
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    • 2013
  • Ribosomal protein (rp) S6 is the substrate of ribosomal protein S6K (S6 kinase) and is involved in protein synthesis by mTOR/S6K/S6 signaling pathway. Some S6 cDNA have been cloned in mammals in recent years but has not been identified in the goat. To facilitate such studies, we cloned the cDNA encoding Cashmere goat (Capra hircus) S6 (GenBank accession GU131122) and then detected mRNA expression in seven tissues by real time PCR and protein expression in testis tissue by immunohistochemisty. Sequence analysis indicated that the obtained goat S6 was a 808 bp product, including a 3' untranslated region of 58 bp and an open reading frame of 750 bp which predicted a protein of 249 amino acids. The predicted amino acid sequence was highly homologous to cattle, human, mouse and rat S6. Expression analysis indicated S6 mRNA was expressed extensively in detected tissues and S6 protein was expressed in testis tissue.

Multilayered frame structure subjected to non-linear creep: A delamination analysis

  • Rizov, Victor I.;Altenbach, Holm
    • Coupled systems mechanics
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    • v.11 no.3
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    • pp.217-231
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    • 2022
  • The present paper is concerned with a delamination analysis of a multilayered frame structure that exhibits non-linear creep behavior. A solution to the strain energy release rate is obtained by considering the time-dependent complementary strain energy in the frame. The mechanical behavior of the frame is treated by using a non-linear stress-strain-time relationship. The time-dependent solution to the strain energy release rate obtained in the present paper holds for a multilayered frame made of arbitrary number of adhesively bonded layers of different thicknesses and material properties. Besides, the dealamination is located arbitrary along the thickness. The solution to the strain energy release rate is verifiedby applying the J-integral approach. A parametric study of the strain energy release rate is carried-out. Two three-layered frame configurations are analyzed in order to evaluate the influence of the delamination crack location along the thickness on the strain energy release rate. The strain energy release is analyzed also for the case when a notch is cut-out in the inner delamination crack arm. The results obtained are compared with these for a frame without a notch.

The metatheatricality of Aurand Harris' plays (오런드 해리스 극의 메타연극성)

  • Yang, Seung-Joo
    • English Language & Literature Teaching
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    • v.15 no.4
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    • pp.313-330
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    • 2009
  • The purpose of this study is to investigate the metatheatricality of Harris' plays marked by the device of 'play-within-a-play' - Androcles and the Lion, Arkansaw Bear, and Punch and Judy. In his metatheatrical plays Harris shows that characters perform for themselves and others. The framework that inner performance is going on within the outer frame play is formed on the stage, and this device reminds the audience in the seats that they are representing reality but that they are only in the middle of performing on the stage. Based on this point of view, this study explores fictionality of character, and play. In Androcles and the Lion, the most-performed children's play in America, the metatheatrical elements are shown in the style of commedia dell'arte, which attacks the rigidity of characters' identity in the Roman society. Another well-known children's play, Arkansaw Bear consists of realistic frame play and fantastic inner play in the mind of a girl, both of which function as a mirror each other and help to sustain aesthetic distance to death and reality on the stage. In Punch and Judy, the structure of frame play and inner puppet play reminds that what's going on in the play is just a fictional play and reflects history of children's puppet show. Harris' unique metatheatricality, the heightened awareness of his own artistic medium, offers children educational opportunity to learn about how a play is performed on the stage and contributes to convey mature theme through children's imaginary participation in the process of playmaking on the stage.

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Development of an Assumed Strain Shell Element for the Three Dimensional Construction Stage Analysis of PSC Bridge (PSC 교량의 3차원 시공 중 해석기법을 위한 가정된 변형률 쉘 요소 개발)

  • Kim, Ki-Du;Song, Sak Suthasupradit;Hwang, Hyun-Jin;Park, Jae-Gyun
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.14 no.3
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    • pp.108-117
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    • 2010
  • The frame element is commonly used for construction stage analysis PSC bridges. However, the frame element does not show sufficient information in the curved PSC box bridges. For the case of curved PSC bridges, the deformations in the inner and outer web are different. In this case, the different jacking forces are required in the inner and outer webs. And it is impossible to calculate different jacking forces in the inner and outer webs if we use the frame element for construction stage analysis. In order to overcome this problem, the use of shell element is essential for a three-dimensional construction stage analysis of PSC bridges. In the following, the formulation of an assumed strain shell element and its application of PSC box girder bridge analysis are presented.

Design of the Stiffened Punch for Stamping of Lead Frame by Buckling Analysis (좌굴해석을 이용한 리드프레임 타발용 펀치의 보강설계)

  • Ko, Dae-Cheol;Lee, In-Soo;Ahn, Byung-Hwan;Kim, Byung-Min
    • Journal of the Korean Society for Precision Engineering
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    • v.23 no.9 s.186
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    • pp.68-75
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    • 2006
  • The lead frame manufactured by stamping process plays an important role in connecting semiconductor to PCB board. As a result of the miniaturization of semiconductor, its corresponding lead frame punch has been narrow. In case of the punch with high slenderness ratio such as lead frame punch, the punch can be broken suddenly due to buckling. To prevent the fracture of lead frame punch, some manufacturers have experientially attached stiffeners to weak parts of punch. The purpose of this study, therefore, is to suggest the guideline far design of stiffened punch. The optimal position and the number of stiffeners to be attached to punch are investigated by elastic buckling analysis. The elastic buckling analysis consists of the eigenvalue buckling analysis and nonlinear buckling analysis. The critical buckling load of elastic buckling analysis is compared with that of buckling test. Finally, the guideline far attaching stiffeners is suggested through analysis of cross section of lead frame punch such as moment of inertia and eccentricity.

Economic Analysis of Connection and Anchorage Methods of Bottom Rebar for Composite Precast Concrete Girder (합성 PC 보의 하부철근 이음 및 정착방법에 따른 경제성 검토)

  • Cho, Wonhyun;Lim, Chaeyeon;Kim, Sunkuk
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2015.05a
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    • pp.11-12
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    • 2015
  • Green Frame is a Rahmen structure made of composite precast concrete members. According to the concrete design code, a lower rebar of precast concrete girder, should be extended to the inner precast concrete column. However, such extension of lower rebar may sharply reduce its constructability. To satisfy the criteria, the connection and anchorage of beam rebar should be taken into consideration, yet it is difficult to use lapping as it is not easy to ensure enough space when Green Frame method is adopted. To solve this, a new method of lower rebar connection and anchorage was developed, and this study is intended to review economic feasibility prior to applying the method developed onto sites. The study result can be used as basic data for selection of the optimal joint and anchorage method for lower rebar of the green frame construction.

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A Study on the Wiring Plan Considering Construction and Frame Performance Degradation of BIPV System (BIPV시스템 시공 및 프레임 성능저하를 고려한 배선처리방안 제시에 관한 연구)

  • Oh, Min-Seok;Kim, Gi-Cheol
    • Journal of the Korean Solar Energy Society
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    • v.38 no.4
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    • pp.33-42
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    • 2018
  • In the application of the BIPV system, it is expected that the workability is lowered due to the difficulty in securing the space for wiring in the frame and the performance of the frame due to the wiring hole processing is lowered. Therefore, In this study, we propose a method to improve the wiring of the inner space of the BIPV frame, and through the simulation evaluation process, the thermal and condensation performance are secured by complementing the problems caused by the hole machining, and the time and effort required for BIPV construction are reduced. For this purpose, a wiring treatment method using a flange insertion tube was proposed, and the thermal and condensation performance was evaluated through simulation analysis.

Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat (Capra hircus)

  • Wang, X.J.;Shi, J.J.;Yang, J.F.;Liang, Y.;Wang, Y.F.;Wu, M.L.;Li, S.Y.;Guo, X.D.;Wang, Z.G.;Liu, D.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.5
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    • pp.606-612
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    • 2012
  • Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal's fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.

An Inductance Voltage Vector Control Strategy and Stability Study Based on Proportional Resonant Regulators under the Stationary αβ Frame for PWM Converters

  • Sun, Qiang;Wei, Kexin;Gao, Chenghai;Wang, Shasha;Liang, Bin
    • Journal of Power Electronics
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    • v.16 no.3
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    • pp.1110-1121
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    • 2016
  • The mathematical model of a three phase PWM converter under the stationary αβ reference frame is deduced and constructed based on a Proportional-Resonant (PR) regulator, which can replace trigonometric function calculation, Park transformation, real-time detection of a Phase Locked Loop and feed-forward decoupling with the proposed accurate calculation of the inductance voltage vector. To avoid the parallel resonance of the LCL topology, the active damping method of the proportional capacitor-current feedback is employed. As to current vector error elimination, an optimized PR controller of the inner current loop is proposed with the zero-pole matching (ZPM) and cancellation method to configure the regulator. The impacts on system's characteristics and stability margin caused by the PR controller and control parameter variations in the inner-current loop are analyzed, and the correlations among active damping feedback coefficient, sampling and transport delay, and system robustness have been established. An equivalent model of the inner current loop is studied via the pole-zero locus along with the pole placement method and frequency response characteristics. Then, the parameter values of the control system are chosen according to their decisive roles and performance indicators. Finally, simulation and experimental results obtained while adopting the proposed method illustrated its feasibility and effectiveness, and the inner current loop achieved zero static error tracking with a good dynamic response and steady-state performance.