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http://dx.doi.org/10.5713/ajas.2011.11290

Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat (Capra hircus)  

Wang, X.J. (College of Life Science, Inner Mongolia University)
Shi, J.J. (College of Life Science, Inner Mongolia University)
Yang, J.F. (College of Life Science, Inner Mongolia University)
Liang, Y. (College of Life Science, Inner Mongolia University)
Wang, Y.F. (College of Life Science, Inner Mongolia University)
Wu, M.L. (College of Life Science, Inner Mongolia University)
Li, S.Y. (College of Life Science, Inner Mongolia University)
Guo, X.D. (College of Life Science, Inner Mongolia University)
Wang, Z.G. (College of Life Science, Inner Mongolia University)
Liu, D.J. (College of Life Science, Inner Mongolia University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.25, no.5, 2012 , pp. 606-612 More about this Journal
Abstract
Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal's fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.
Keywords
Cashmere Goat; IGFBP-5; Expression Pattern; Tissue-specific; Hair; Gene;
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