• 한국식품영양과학회지
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• 제14권1호
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• pp.1-12
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• 1985

가열 및 동결저장이 오징어, 굴, 새우 및 명태의 in vitro 소화율과 trypsin indigestible substrate (TIS) 함량 변화에 미치는 영향을 실험한 결과는 다음과 같다. Boiling 했을 때 최고의 운 in vitro 소화율을 나타내는 시간은 오징어 1분, 내장을 제거한 굴 0.5분, 내장을 제거하지 않은 굴은 1분이었고, 명태는 5분 이었으며, 오징어와 굴은 부위에 따라 소화율은 다르게 나타났다. 최고의 in vitro 소화율을 나타내는 steaming 조건은 오징어는 $100^{\circ}C$에서 1분, 굴은 $88^{\circ}C$에서 1분이었으며, 명태는 $100^{\circ}C$에서 $1{\sim}2.5$분이었다. TIS는 진시료에서 in vitro 소화율과 역상관관계를 가지며 변화하였다. 동결건조한 시료가 다른 건조품보다 현저하게 높은 in vitro 소화율을 보였으나, 내장을 제거했거나 지방함량이 낮은 시료를 천일건조했을 때도 동결건조시료에 버금가는 in vitro 소화율을 나타내었다. 동결저장은 지방함량이 낮은 시료의 in vitro 소화율의 저하 및 TIS함량 증가에 효과적이었으나, 지방함량이 높아 산패가 심하게 진행된 시료에는 현저한 효과를 볼 수 없었다. 지방함량은 수산식품단백질을 가열처리했거나 저장했을 때의 소화율 변화에 결정적인 영향을 미침을 알 수 있었으며, 4가지의 효소를 이용한 multi-enzyme digestion technique은 수산식품단백질의 소화율 변화를 측정함에 있어 아주 민감하고 유용한 방법으로 판명되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제12권1호
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• pp.15-21
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• 1999

The aim of the present study was to determine the effect of paternal sex chromosome on early development of buffalo embryos fertilized and cultured in vitro. Embryos were produced in vitro from abattoir derived buffalo oocytes. The cleaved embryos were cocultured with buffalo oviductal epithelial cells and evaluated on day 7 under the phase contrast microscope to classify development. The embryos which reached the morula/blastocyst stage were fast developing, the embryos which were at 16-32 cell stage were medium developing and the embryos below 16 cell stage were slow developing. The embryos which showed some fragmentation in the blastomeres or degenerated blastomeres, were degenerating. Sex of emberyos (n=159) was determined using PCR for amplification of a male specific BRY. 1 (301 bp) and a buffalo specific satellite DNA (216 bp) fragments. The results thus obtained show that 1) X and Y chromosome bearing sperms fertilize oocytes to give almost equal numbers of cleaved XX and XY embryos, 2) male embryos develop faster than female embryos to reach advanced stage and 3) degeneration of buffalo embryos is not linked with the paternal sex chromosome. We suggest that faster development of males is due to differential processing of X and Y chromosome within the zygote for its activation and / or differential expression of genes on paternal sex chromosome sex chromosome during development of buffalo embryos fertilized and cultured in vitro which may be attributed to a combination of genetic and environmental factors.

• Rapid Communication in Photoscience
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• 제5권1호
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• pp.11-12
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• 2016

Schwann cells and neuronal cells from dorsal root ganglion (DRG) in embryos of rat were cultured in vitro respectively. The purified neuronal cells with anti-mitotic agents and purified Schwann cells were co-cultured and then accomplished myelination processing. Infection of Semliki forest virus into this myelinated co-culture system was performed and then accomplished demyelination. We identified myelination and demyelination processing using antibody of neuropeptide Y.

• 한국수산과학회지
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• 제17권2호
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• pp.101-108
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• 1984

소화율이 우수한 자건멸치의 가공조건을 규명하기 위하여 자숙시간과 건조방법을 달리하여 제조한 멸치를 저온($9{\pm}1^{\circ}C$) 및 고온($26{\pm}1^{\circ}C$)에서 저장했을 때의 trypsin 활성저해물질(TI)과 시험관적 단백질 개산소화율의 변화를 측정하고, 동시에 이에 영향을 미친다고 생각되는 지방의 산패 및 갈변도의 변화를 분석 검토하였다. 자숙시간에 따른 TI의 생성정도는 5분간 자숙한 시료가 가장 적었고, 건조방법을 달리 했을 때는 열풍 건조시료가 일건 및 동결건조시료보다 낮은 값을 나타내었다. 그리고, 저온($9{\pm}1^{\circ}C$)에서 저장한 시료는 고온($26{\pm}1^{\circ}C$) 저장시료보다 낮은 값을 보였으며, 5분간 자숙 후 천일건조한 시료의 경우는 저온저장 56일에 0.173mg/g의 TI를 나타내어 전 시료 중에서 가장 낮은 값을 보였다. 소화율은 0.5분간 자숙하여 천일건조한 경우는 $85.3\%$, 1분간 자숙 후 동결건조했을 때 $85.9\%$로 가장 높았으며, 저온저장($9{\pm}1^{\circ}C$)한 것이 고온저장($26{\pm}1^{\circ}C$)한 것 보다 감소정도가 적어 저장 56일 후에도 $2.5\%$ 밖에 감소하지 않았다. 지방의 산패는 자숙조건별로 볼 때 5분간 자숙한 시료에서, 건조방법별로는 동결건조한 시료에서 가장 촉진되었고, 갈변은 생시료 또는 0.5분간 자숙하여 일건한 뒤 고온($26{\pm}1^{\circ}C$)에서 저장한 시료가 가장 촉진되 었다. 소화율은 저장기간이 경과함에 따라 저하하는 반면, TI의 생성정도 지방의 산패정도 및 비효소적 갈변도도 증가하였다. 결과적으로 지방의 산패 및 비효소적 갈변반응이 TI의 생성 및 소화율적 저하에 깊이 관여하는 것으로 생각되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제33권8호
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• pp.1292-1300
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• 2020

Objective: To explore feed resources capable of replacing regular poor-quality fodder, this study was conducted to evaluate the effects of additives on the fermentation quality, in vitro digestibility and aerobic stability of mulberry leaves silage. Methods: The mulberry leaves were ensiled either untreated (control) or treated with 1×10⁶ cfu/g fresh matter Lactobacillus plantarum (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and Lactobacillus plantarum (L+G), and a combination of 3% molasses and Lactobacillus plantarum (L+M). The fermentation quality and chemical composition were analyzed after 7, 14, 30, and 60 d, respectively. The 60-d silages were subjected to an aerobic stability test and fermented with buffered rumen fluid to measure the digestibility. Results: Inoculating lactic acid bacteria (LAB) resulted in more rapid increase in lactic acid concentrations and decline in pH of mulberry leaves silage as compared control. Higher acetic acid and lower ethanol and ammonia nitrogen concentrations (p<0.05) were observed in the LAB-inoculated silages as opposed to control during ensiling. The LAB-inoculated silages contained lower water-soluble carbohydrates compared with control during the first 14 d of ensiling, and lower neutral detergent fibre (p<0.05) concentrations as compared with non-LAB inoculated silages. Adding molasses alone increased (p<0.05) the digestibility of dry matter (DM). The aerobic stability of mulberry leaves silage was increased by LAB inoculation, whereas decreased by adding glucose or molasses. Conclusion: The LAB inoculation improved fermentation quality and aerobic stability of mulberry leaves silage, while adding glucose or molasses failed to affect the fermentation and impaired the aerobic stability. Inoculating LAB alone is recommended for mulberry leaves especially when ensiled at a relatively high DM.

• Asian-Australasian Journal of Animal Sciences
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• 제13권9호
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• pp.1337-1344
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• 2000

Differences in the physical structure and chemical composition of sorghums result in different nutritional values. Sorghums with high in vitro nutrient digestibility tend to have greater ileal and total tract nutrient digestibilies. Soft endosperm can improve growth and nutrient digestibility in nursery pigs and broiler chicks. However, finishing pigs respond less to endosperm hardness. Chicks benefit from waxy sorghums, but responses of swine to waxy sorghum remain controversial. Reduction of particle size benefits nursery pigs more than finishing pigs, while age of chicks affects the coarseness preference. Nutritional benefits of thermal processing in sorghum remain unclear in chicks and pigs. Although experiments have demonstrated increased efficiency with processed sorghum, processing provided only an immediate solution to the problem of reduced utilization. Long-term, solutions will be genetic improvement of physical and on chemical characteristic.

• KSBB Journal
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• 제13권4호
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• pp.369-377
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• 1998

To analyze the unique aspects of biomolecular processing for monoclonal antibody (MAb) production and secretion, the simple working model based on 3-compartment (endoplasmic reticulum, Golgi apparatus, and extracellular medium) was developed. Based on in vitro MAb assembly experimental results, the kinetic model for MAb assembly in the endoplasmic reticulumn was proposed. The dynamics of MAb assembly and secretion was simulated using methematica program. According to the simulation results, the proposed 3-compartment model provides an efficient means to predict the specific MAb productivity as well as intracompartmental concentrations of MAb in endoplasmic reticulum, Golgi apparatus, and extracellular compartment model. In vivo profiles of MAb intermediates gave good agreements with the simulation profiles predicted by the intracellular compartment model. Furthermore, results of such analysis can help in directing the control strategy for optimum biomolecular processing in a mammalian cell culture system.

• 생명과학회지
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• 제19권1호
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• pp.65-74
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• 2009

본 연구는 미국산 옥수수와 인도산 옥수수의 steam flaking 처리가 in vtro 가스발생량과 미생물 성장량 그리고 곰팡이 독소 aflatoxin $B_1$과 ochratoxin A의 농도에 미치는 영향을 구명하기 위하여 수행되었다. 실험 설계는 4개의 처리구, (1) USCW (미국산 무처리 옥수수), (2) USCF (미국산 steam flaking 옥수수 ), (3) IDCW (인도산 무처리 옥수수) 그리고 (4) IDCF (인도산 steam flaking 옥수수), 처리구당 4반복으로 6개의 발효시간대(3, 6, 9, 12, 18 및 24)를 두고 in vitro 실험을 수행하였다. 공시한 옥수수중 aflatoxin $B_1$이나 ochratoxin A와 같은 곰팡이 독소의 함량은 항구, hopper, 사일로 그리고 가공 전까지 보관기간이 길어짐에 따라 증가하는 경향을 보였다. 공정라인별로 곰팡이 독소를 측정한 결과 입고 시 인도산 옥수수(IDCW)와 미국산 옥수수 (USCW)의 aflatoxin $B_1$ 수치는 각각 11.71 ppb와 1.78 ppb으로 나타났지만 steam flaking 후의 aflatoxin $B_1$ 함량은 USCW 구와 IDCW구에서 전혀 검출되지 않아(0.00 ppb) steam flaking 처리가 곰팡이 Aspergillus flavus를 감소시킬 수 있는 것으로 조사되었다. 그러나 이러한 경향은 ochratoxin A 함량에서 관찰되지 않았다. In vitro 실험에서 gas 발생량은 원산지별로는 미국산 옥수수 (USCW & USCF)가 인도산 옥수수 (IDCW & IDCF) 보다 유의적으로 높았으며 가공 처리별로는 steam flaking 처리한 옥수수가 알곡 옥수수보다 발효 3시간대를 기준으로 $1.5{\sim}2%$ 정도 높았다. 배양액 중의 pH 는 $6.05{\sim}6.54$의 범위로서 미생물이 성장하기에 적정한 pH를 유지하였으며 처리 구간에 유의적인 차이는 찾아 볼 수 없었으나 USCF구의 pH가 다른구에 비해 다소 낮았다. pH는 배양 12 시간까지 감소하였으며 이 시간 중에 가스 발생량은 급격히 증가하였다. In vitro 미생물 성장량도 발효 18시간까지 증가하다가 그 이후 시간대에서는 성장량이 증체를 보이거나 오히려 감소하는 경향이었다. 결론적으로 원산지별로는 in vitro 실험결과와 곰팡이 독소 함량을 기준으로, 미국산 옥수수가 인도산 옥수수보다는 품질이 훨씬 높았으며, 수입산 옥수수의 steam flaking 처리는 invitro 가스발생량 및 미생물 성장량을 개선 시킬 뿐만 아니라, aflatoxin $B_1$이나 ochratoxin A와 같은 곰팡이 독소를 감소시키는 역할을 하였다.

• Macromolecular Research
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• 제17권12호
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• pp.1025-1031
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• 2009

Several kinds of biodegradable hydrogels were prepared via in situ photopolymerization of Pluronic F127/poly($\varepsilon$-caprolactone) macromer and acrylic acid (AA) comonomer in aqueous medium. The swelling kinetics measurements showed that the resultant hydrogels exhibited both thermo- and pH-sensitive behaviors, and that this stimuli-responsiveness underwent a fast reversible process. With increasing pH of the local buffer solutions, the pH sensitivity of the hydrogels was increased, while the temperature sensitivity was decreased. In vitro hydrolytic degradation in the buffer solution (pH 7.4, $37^{\circ}C$), the degradation rate of the hydrogels was greatly improved due to the introduction of the AA comonomer. The in vitro release profiles of bovine serum albumin (BSA) in-situ embedded into the hydrogels were also investigated: the release mechanism of BSA based on the Peppas equation was followed Case II diffusion. Such biodegradable dual-sensitive hydrogel materials may have more advantages as a potentially interesting platform for smart drug delivery carriers and tissue engineering scaffolds.

• Journal of Microbiology and Biotechnology
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• 제1권3호
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• pp.212-219
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• 1991

Four species of yeast, Saccharomyces cerevisiae, Candida utilis, Saccharomycopsis flbuligera, and Schwanniomyces castellii were evaluated for their ability to bioconvert potato processing waste water into microbial protein and the resulting single-cell proteins were evaluated as protein sources for rainbow trout, using in vitro analyses. The studies indicated that Schwanniomyces castellii, which utilizes starch dircetly and converts it into cell mass efficiently, was suitable for the bioconversion. In the single-stage continuous bioconversion, the yield S. castellii cell mass, which contained approximately 37% protein, was 77%, at dilution rate 0.25 $h^{-1}$. Reduction of total carbohydrate was 81%. During batch fermentations, cell mass yield was about 72% and total carbohydrate reduction was 81%. Among the yeasts tested, S. castellii possessed the most fragile cell wall and had a favorable amino acid profile for salmonid fish; protein score of 86% (Met). In an in vitro pepsin digestibility test 80% digestibility (23~38% above control) was observed when cells were pre-heated in a steam bath for 30 min. Results presented should be regarded as being preliminary in nature because they were derived from single experiments.