• Title/Summary/Keyword: in vitro protein digestibility

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A Very Early-Maturing Italian Ryegrass (Lolium multiflorum Lam.) New Variety, 'Greenfarm3ho' (이탈리안 라이그라스 신품종 '그린팜 3호'의 생육특성과 수량성)

  • Ji, Hee Chung;Whang, Tae Young;Kim, Ki-Yong;Choe, Hyunsoak;Hong, Ki Hung;Choe, Kuh Wann;Lee, Ki-Won;Lee, Sang-Hoon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.35 no.1
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    • pp.31-35
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    • 2015
  • This experiment was carried out to breed a very early-maturing variety of Italian ryegrass (Lolium multiflorum Lam.) in the Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan, Korea from 2012 to 2014. New variety "Greenfarm3ho" is a diploid variety with a green leaf color, a semi-erect growth habit in late autumn/fall, and an erect growth habit in early spring. With a heading date of April 27, Greenfarm3ho was categorized as an early-maturing variety. Compared with the "Greenfarm" control variety, Greenfarm3ho's flag-leaf width was narrower by 0.5 mm, its flag-leaf length was longer by 4.4 cm, and its plant length was longer by 3 cm; additionally, Greenfarm3ho's stem thickness was 0.1 mm thicker and it showed winter hardiness. The dry matter (DM) yield (8,976 kg/ha) of Greenfarm3ho was similar to that of Greenfarm. The in vitro dry matter digestibility (IVDMD), total digestible nutrient (TDN), and crude protein (CP) of Greenfarm3ho were 70.0%, 64.1%, and 1.0%, respectively, which are higher than those of Greenfarm by 2.9%, 2.0%, and 1.4%, respectively. The acid detergent fiber (ADF) and neutral detergent fiber (NDF) content of Greenfarm3ho were 31.4% and 53.3%, respectively, which are lower than those of Greenfarm by 2.5% and 3.0%, respectively.

Effect of Harvest Stage of Corn on Nutritive Values and Quality of Roll Baled Corn Silage Manufactured with Corn Grown in Paddy Land (논에서 생산된 옥수수의 수확시기가 곤포사일리지의 사료가치와 품질에 미치는 영향)

  • Choi, Ki-Choon;Jo, Nam-Chul;Jung, Min-Woong;Lee, Kyung-Dong;Kim, Jong-Geun;Lim, Young-Chul;Kim, Won-Ho;Oh, Yung-Keun;Choi, Jin-Hyuk;Kim, Cheon-Man;Jung, Du-Keun;Choi, Jong-Man
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.1
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    • pp.65-74
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    • 2011
  • This study was carried out to examine the effect of harvest stage of corn on nutritive values and quality of round baled corn silage manufactured with corn grown in paddy land of Department of Animal Resources Development, National Institute of Animal Science, RDA from 2009 to 2010. Corn "Kwangpyungok" was harvested at three different growth times (milk, yellow ripen and ripen stage) and ensiled at each harvest stages. Crude protein (CP) and TDN (total digestible nutrient) contents of round baled corn silage was decreased and in vitro dry matter digestibility (IVDMD) was not changed with delayed harvest maturity. However, contents of ADF (acid detergent fiber) and NDF(neutral detergent fiber) decreased with delayed harvest maturity. The pH at three different harvest stages ranged from 3.8 to 4.0. The content of lactic acid increased with delayed harvest maturity, but the content of acetic acid decreased. And then, flieg's score reveals that there is an increase in order, ripen stage > yellow ripen stage > milk stage. Therefore, this study suggest that round baled corn silage manufactured at yellow ripen stage can improve the silage quality.

Evaluation of Feed Value for Cow Manure-Sawdust Mixtures Fermented by a Fungal Mycelium of Formitella flaxinea (Formitella flaxinea에 의하여 발효된 우분-톱밥 배양물의 사료가치 평가)

  • Kim, Yong-Kook
    • Journal of the Korea Organic Resources Recycling Association
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    • v.14 no.1
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    • pp.103-111
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    • 2006
  • Fresh dairy cow manure was mixed with dried sawdust at the following moisture contents and manure: sawdust ratios: 50% and 57:43 ($\underline{M50}$), 55% and 64:36 ($\underline{M55}$), 60% and 70:30 ($\underline{M60}$), 65% and 76:24 ($\underline{M65}$), 70% and 83:17 ($\underline{M70}$) 75% and 90:10($\underline{M75}$) and 80% and 96:04($\underline{M80}$). The mixtures were fermented by a fungal mycelium of Fomitella flaxinea for 2wk at 29 C to recycle cow manure along with sawdust and fungal mycelium as a ruminant feedstuff. Chemical composition and in vitro rumen dry matter digestibilities of fermented mixtures were compared with unfermented mixture. The crude protein contents of mixtures were not changed by fermentation with fungal mycelium. Neutral detergent fiber contents of 4WK fermented mixtures (90.6, 85.3, 80.4, and 76.4% for $\underline{M50}$, $\underline{M60}$, $\underline{M70}$ and $\underline{M80}$, respectively were lower (P<0.05) than those of unfermented mixtures (91.1, 89.9, 84.3, and 79.4%). However, acid detergent fiber contents of fermented mixtures (73.8, 68.9, 65.3, and 58.0%) were higher (P<0.05) than those unfermented mixtures (70.2, 67.8, 61.7, and 56.3%). In vitro rumen dry matter digestibilities of fermented mixtures for four weeks(49.4, 36.8, 28.6, and 22.3% for $\underline{M50}$, $\underline{M60}$, $\underline{M70}$ and $\underline{M80}$) were higher than those of unfermented mixtures(34.1, 27.5, 20.6, and 15.4%) (P<0.05).

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Effect of Cutting Height on Productivity and Forage Quality of Alfalfa in Alpine Area of Korea

  • Kim, Hak Jin;Li, Yan Fen;Jeong, Eun Chan;Ahmadi, Farhad;Kim, Jong Geun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.41 no.3
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    • pp.147-154
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    • 2021
  • Cutting management has been identified as a critical factor in the alfalfa production systems because it has a significant impact on maximizing yield and maintaining the forage quality. The objective of this experiment was to determine the proper cutting height according to harvesting time for optimizing nutrient yield and forage nutritive quality of alfalfa grown in alpine regions of Korea. Alfalfa was sown at a seeding rate of 30 kg/ha in August 2018 and harvested at four cuttings in 2019 (3 May, 2 July, 11 September, and 13 October). Cutting heights were adjusted at 5, 15, and 25 cm above the soil surface. Alfalfa plant was tallest at the third cutting (109 cm), which was on average 35 cm taller than the first or second cutting. Relative feed value (RFV) remained unaffected by cutting height at the first harvest, but increased consistently in subsequent harvests as cutting height increased. Alfalfa collected at the first and fourth cuttings had the highest RFV (mean 152), which was on average 8 and 67 units higher than the second and third harvests, respectively. At each harvest, in vitro dry matter digestibility was highest in alfalfa cut at a 25-cm height. Dry matter (DM) production at each cutting height was highest in the first cutting, accounting for on average 36-37% of total annual DM production, and lowest in the fourth harvest, accounting for about 11-13% of the total DM yield. The total dry matter production (in four harvests) was 4,218 kg/ha higher when alfalfa was subjected to a cutting height of 5 cm rather than 25 cm. Cutting height had no effect on total crude protein yield, but from the first to fourth cutting, the protein yield followed a decreasing trend. Finally, there were visible declines in forage nutritive quality when alfalfa was cut at a shorter height. However, the magnitude of difference in total forage yield may outweigh the slight decline in forage quality when alfalfa is cut at a lower height. The findings of this study could help the alfalfa growers make better harvest management decisions.

Effect of Seed Blending Rates Between Rye and Barley on Forage Production and Quality in Daejon Area (대전지역에서 호밀과 보리의 파종비율이 건물수량 및 사료가치에 미치는 영향)

  • Lee, In-Duk;Lee, Hyung-Suk;Shin, Yeun-Ho
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.28 no.4
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    • pp.289-294
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    • 2008
  • This experiment was conducted at college of Agriculture and Life Science in Chungnam National University from September, 2004 to June, 2007 in order to evaluate the mixture possibility between barley and rye in the area of Daejeon. Rye (cultivar; Koolgrazer) and barley (cultivar; Daeyeon Bori) were set for the experiment. The experiment was arranged in four treatments: R100 (rye 100%), R60 + B40(rye 60% + barley 40%), R50% + B50% (rye 50% + barley 50%), and R40% + B60% (rye 40% + barley 60%). The experiment was repeated three times in the randomized complete block. The average dry matter (DM) yield for three years of R100 weighed 9,282 kg and its DM yield was higher than any other DM yield. The higher the barley seed rates are, the lower the DM yield is (p<0.05). As the barley seed rates increased 40%, 50%, and 60% respectively, its vegetative percentage tended to increase 30%, 41%, and 47%, but the barley vegetative percentage against its seed rates did bring forth somewhat low results. Compared with R100, the contents of crude protein (CP) and in vitro dry matter digestibility (IVDMD) got higher as the barley seed rates became higher, while the contents of NDF, ADF, cellulose, and lignin were lower (p<0.05). Compared with R100, the yields of crude protein dry matter (CPDM) and digestible dry matter (DDM) showed lower in the any mixed barley (p<0.05). Thus, in case of using barley mixed with rye in the area of Daejeon, it seems to be quite difficult, unless the supply of high-productive barley variety is followed, to enhance the yields of DM, CPDM, and DDM.

Optimizing Cooking Condition of Short Grain Rice Containing Sea-tangle Patch (다시마밥 제조조건의 최적화)

  • Shin, Eun-Soo;Lee, Jin-Hwa;Park, Kyong-Tae;Ryu, Hong-Soo;Jang, Dae-Heung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.10
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    • pp.1726-1734
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    • 2004
  • Response surface methodology on sensory evaluation was used to optimize cooking condition of cooked short grain rice (CSR) and cooked short grain rice containing grainy sea-tangle patch (CSTR). Texture profile analysis and nutritional evaluation were also performed. For maximizing overall acceptability, it can be predicted sea-tangle patch 14%, added water 1.67 times of rice and sea-tangle amount and steeping time 47 min as optimum cooking conditions from sensory evaluation conducted by the forties panelist. Hardness of pressure cooked short grain rice containing grainy sea-tangle patch (CSTR) were lower than cooked short grain rice (CSR), but it showed similar as CSR in adhesiveness, chewiness and higher in springiness and cohesiveness. In addition CSTR was delayed retrogradation compared with CSR. The nutritional Quality of CSTR showed higher level in total dietary fiber, protein, ash than CSR, but lower in protein digestibility, C-PER, gelatinization degree compared with CSR.

A Study on Rumen Fermentation Characteristics and Greenhouse Gas Emission of Forages in South Korea (국내 조사료별 반추위 내 발효특성과 온실가스 발생량에 관한 연구)

  • Young Ho Joo;Ji Yoon Kim;Myeong Ji Seo;Chang Hyun Baeg;Seung Min Jeong;Sam Churl Kim
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.4
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    • pp.268-273
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    • 2023
  • The present study estimated rumen fermentation characteristics and greenhouse gas emissions of different forages. Alfalfa, timothy, tall fescue, Italian ryegrass, and rice straw as the main forage sources for Hanwoo were used in the present study. Crude protein was highest in alfalfa but lowest in rice straw (p<0.05). Ether extract was higher in alfalfa and Italian ryegrass than in the other forages (p<0.05). Crude ash was highest in rice straw but lowest in tall fescue (p<0.05). Neutral detergent fiber was highest in tall fescue but lowest in alfalfa (p<0.05). Acid detergent fiber was highest in Italian ryegrass and rice straw but lowest in alfalfa (p<0.05). In vitro digestibilities of dry matter (DMD) and neutral detergent fiber (NDFD) were highest in timothy but lowest in rice straw (p<0.05). Rumen pH was highest (p<0.05) in alfalfa, while ammonia-N was higher (p<0.05) in alfalfa and Italian ryegrass than in the other forages. Total volatile fatty acid was highest (p<0.05) in timothy, while acetate and propionate were highest (p<0.05) in alfalfa and rice straw, respectively. Acetate to propionate ratio was higher (p<0.05) in alfalfa, timothy, and Italian ryegrass than in rice straw. Rice straw had lowest total gas (mL) (p<0.05) but highest its per DMD and NDFD. Rice straw had higher (p<0.05) CO2 (per DMD and NDFD) compared to alfalfa (per DMD and NDFD), timothy (per DMD and NDFD), tall fescue (per NDFD), and Italian ryegrass (per DMD). Again, rice straw had higher (p<0.05) CH4 (per DMD and NDFD) compared to timothy (per DMD and NDFD) and tall fescue (per NDFD). Therefore, this study indicates that timothy has a higher nutrient digestibility and volatile fatty acid in the rumen leading to a reduction of greenhouse gas emission.

Forage Productivity and Quality of Domestic Italian Ryegrass and Barley Varieties (국내 개발 이탈리안 라이그라스와 청보리 주요 품종의 생산성과 사료가치 비교)

  • Seo, Sung;Kim, Won-Ho;Kim, Ki-Yong;Choi, Gi-Jun;Ji, Hee-Chung;Lee, Sang-Hoon;Lee, Ki-Won;Kim, Meing-Jooung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.3
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    • pp.261-268
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    • 2011
  • This study was carried out to determine the forage production and quality of Italian ryegrass (IRG) and forage barley developed by Korea in Suwon, 2009~'10. The nine treatments were two IRG varieties (Kowinearly with early maturity and Kowinmaster with medium maturity), five barley varieties (Youngyang, Wooho, Yuyeon, Dami and Youho), and two mixtures (Kowinearly + Yuyeon and Kowinmaster + Yuyeon). The heading dates of Kowinearly and Kowinmaster were 14 May and 18 May, respectively. The growth stage of barley investigated at 22 May were late milk in Youngyang and Wooho, early dough in Dami and early to medium dough in Yuyeon and Youho. Plant length of IRG in IRG + barley mixtures was 117~118 cm, which was longer than those of IRG monoculture of 98~101 cm, and no lodging was found in mixtures. The dry matter (DM) percentage at harvest was 20.7~25.4% in all treatments. The botanical composition of IRG in mixtures was 43.1%. The percentage of spike per barley plant was become high according to progressed maturity, as a 35.7%, 44.1%, 54.8% and 57.2% in late milk, dough, yellowish and full ripeness stage, respectively, and the spike percentages of Youngyang and Wooho were tends to high. The crude protein (CP) content of IRG as 9.0~10.0% was higher than that of barley (7.0~8.5%), and the contents of NDF and ADF of barley were lower than those of IRG, and in vitro DM digestibility were 64.4% in Kowinearly, 64.1% in Kowinmaster, 64.5% in mixture, and 60.2% (Youho) to 66.4% (Wooho) in barley. The yields of DM, CP and in vitro digestible DM were high in Kowinmaster+barley mixture as a 11,508 kg, 1,046 kg and 7,422 kg per ha, respectively (p<0.05). However, no significant differences in forage yield were observed among cultivar of IRG, and barley, although Wooho was tends to high in digestibility and forage yield among five barley varieties. In conclusion, the mixture cultivation of IRG Kowinmaster + forage barley was recommended, because of preventing of IRG lodging, higher plant length of IRG, increasing of forage yield, and stable production. Selection of suitable winter forage species and variety for district, climate environment, and utilization type of farm was also important.

Evaluation of Characteristics, Winter Survival and Forage Production for Warm Season Grass in the Mid-Southern Regions of Korea (중남부지역에서 난지형 목초의 생육특성, 월동성 및 사초생산성 평가)

  • Park, Hyung Soo;Jung, Min Woong;Jung, Yong Bok;Lim, Young Chul;Choi, Ki Choon;Kim, Ji Hye;Lee, Ki Won;Choi, Gi Jun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.34 no.1
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    • pp.1-8
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    • 2014
  • Field studies were conducted from the years 2009 to 2012 in order to determine the cultivation limit as well as to evaluate the characteristics and forage production of warm season grass in Korea. Two bermudagrass [Cynodon dactylon (L.) Pers.] cultivars, two bahiagrass (Paspalum notatum Flugge) cultivars and a Kleingrass [Panicum coloratum L.] cultivar were compared for forage production and quality in the mid-southern regions of Korea. The experimental design was a randomized block design (RBD) with three replications. The number of days to seedling emergence for bremudagrass and bahiagrass was observed as approximately 12 days and 28 days after seeding, respectively. In Kwangju, the heading dates of bahiagrass and kleingrass were 21 August and 10 July, respectively,. Warm season grass did not winter in the mid-regions (Kimjea, Cheonan) of Korea. All of the Bermudagrass cultivars had higher dry matter (DM) than bahiagrass at the first harvest. The dry matter yield of kleingrass was usually greater than the other entries at all study sites. Peak forage DM production of bermudagrass and bahiagrass cultivars occurred in June and July, respectively. The contents of crude protein (CP) and total digestibility nutrient (TDN) for bermudagrass cultivars were usually greater than the other entries at all study sites. Further, acid detergent fiber (ADF) and in vitro DM digestibility (IVDMD) were similar across all cultivars.

Effects of Fibrolytic Enzyme Addition on Ruminal Fermentation, Milk Yield and Milk Composition of Dairy Cows (Fibrolytic Enzyme 첨가가 반추위 발효 성상 및 착유우의 유량 및 유성분에 미치는 영향)

  • Ahn, J. H.;Kim, Y. J.;Kim, H. J.
    • Journal of Animal Science and Technology
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    • v.45 no.1
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    • pp.131-142
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    • 2003
  • We evaluated the effects of adding fibrolytic enzyme into ruminant diets on ruminal fermentation (in vitro) and lactational performances of dairy cows (in vivo). Through the in vitro experiment that was carried out with different contents of NDF (34, 38, 43%) in diets, digestibilities of NDF in the rumen appeared not significantly different by the addition of enzyme but were different by NDF content in diets showing higher digestibility in NDF 43% diet. It could be attributed by the relatively higher amount of hemicellulose in the current experimental diets than in conventional diets that might have been digested easily by the addition of fibrolytic enzyme in the rumen. The addition of fibrolytic enzyme tended to increase NDF digestibilities to a little extent both in 0.05 and 0.1% enzyme levels. Ruminal pH, NH3-N concentrations and VFA production in the rumen were not affected by the addition of fibrolytic enzyme. Activities of CMCase and xylanase were higher in enzyme treated diets of both NDF 34 and 38%. In particular, the activities of xylanase that slowly decreased from 0 to 12 hr but rapidly after 24 hr indicates that the major action of the enzyme in the rumen occurs in early period of incubation. Through an in vivo experiment, fibrolytic enzyme addition into the diets of dairy cows indeed affected lactational performance of milk yield. The cows fed enzyme treated diets produced 8% (1.9kg/d) more amounts of milk than with no enzyme addition. Milk composition of milk fat and protein was not affected by enzyme addition. Overall, the results of this in vivo study indicates that fibrolytic enzyme can be used to improve milk production in lactating cows. In respect that animals in different treatments of this study had the same amounts of intake, the increased milk yield with enzyme addition may be attributed to the improved utilization of nutrients in the digestive tract.