• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.26-31
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• 2016

Toxoplasmosis is an important cause of foodborne, inflammatory illnesses, as well as congenital abnormalities. Currently available therapies are ineffective for persistent chronic disease and congenital toxoplasmosis or have severe side effects which may result in life-threatening complications. There is an urgent need for safe and effective therapies to eliminate or treat this cosmopolitan infectious disease. The aim of this study was to investigate the in vitro anti-Toxoplasma activities of Plantago asiatica L., one of the herbal extracts, using tachyzoit of T. gondii RH strain infected HeLa cells. As the results, the selectivity of Plantago asiatica L. extract was 4.5, which was higher than Sulfadiazine selectivity (0.4). Also, we perfomed the cell proliferation inhibition test and the morphological study to evaluate the anti-T. gondii activity of Plantago asiatica L. extract with HeLa cells. As the results, the inhibition rate of the Plantago asiatica L. extract was high inhibition rate. This indicates that the Plantago asiatica L. extract may be used for new anti-T. gondii agent.

• Korean Journal of Food Science and Technology
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• v.37 no.1
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• pp.67-72
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• 2005

Antioxidative activity of crude saponin fraction (CSF) prepared from Basidiomycota cultured with fresh ginseng (Panax ginseng C.A. Meyer) as substrate was investigated by analyzing CSFs for ginsenoside and phenolic compounds. On TLC chromatogram, ginsenosides such as $Rg_{2},\;Rg_{3}$, and $Rh_{1}$ which were rare in fresh ginseng, were identified. CSF of Phellinus linteus culture product showed the highest total phenolic content and electron donating ability (EDA), suggesting phenolic compounds contribute to EDA. In vitro lipid peroxidation was inhibited most by CSF of Ganoderma lucidum, indicating that the highest EDA does not imply highest inhibition against lipid peroxidation. Tyrosinase was also inhibited mostly by CSF of G. lucidum. These results suggest culture of Basidiomycota with fresh ginseng has more active substances than fresh ginseng alone.

• Journal of dental hygiene science
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• v.14 no.3
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• pp.405-410
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• 2014

The purpose of this study in vitro investigation was to compare the marginal and internal fit of Ni-Cr alloy metal ceramic crown before and after porcelain veneering. Furthermore, this study evaluated whether the influence of the porcelain firing on the precision of fit of dental prostheses. The maxillary right incisor was selected as an abutment for experiments. Ten working models were prepared. Ni-Cr alloy cores appropriate for each abutment were prepared by lost wax technique. The marginal area and four internal areas of the crowns were measured at two stages: before veneering process and after upper porcelain firing. Silicone replica techniques were used. The data were statistically analyzed with the paired t-test (${\alpha}=0.05$). $Mean{\pm}SD$ marginal and internal gap were $67.1{\pm}23.3{\mu}m$ for the nickle chrome alloy core group and $74.4{\pm}21.9{\mu}m$ for the metal ceramic crown group. There were statistically significant differences in all investigated areas (p<0.05). Within the limitations of this study, none of the Ni-Cr alloy metal crown values measured after porcelain firing process exceeded $120{\mu}m$, which is the clinically acceptable threshold.

• Proceedings of the Ginseng society Conference
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• 1988.08a
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• pp.115-121
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• 1988

Although Saponin A from Panax ginseng has previously been shown to inhibit carageenin induced edema. a paucity of information exists on the effects of components from ginseng on the cellular inflammatory response. specifically polymorphonuclear leukocyte (PMNL) function. The purpose of this study was 10 determine the effects of isolated neutral dammarane saponins from ginseng (i.e..glycosidic derivatives of 20(S)-protopanaxadiol [ginsenoside $Rb_1,\;Rb_2$ and Rc] and 20(S)-protopanaxatriol [ginsenosides Re and $Rg_1$]) on in vivo PMNL function and to compare their effects with those produced by a steroidal anti-inflammatory agent (dexamethasone) and commercially available saponin. Dexamethasone. the ginsenosides and saponin were all shown to he potent inhibitors of PMNL chemotaxis using the $^{51}Cr$ assay with $5{\times}10^{-8}M$ f-met-leu-phe [FMLP] as the chemoattractant. Inhibition or PMNL chemotaxis by dexamethasone. the ginsenosides and saponin were all shown to be both time-and dose-dependent and these agents did not affect cellular viability at the concentrations tested Saponin and the ginsenosides were more potent inhibitors of chemotaxis than was dexamethasone. while oxidant generation (as measured by the luminol-enhaneed chemil-uminescence of PMNL using FMNL $[10^{-6}]$ as the stimulus) was inhibited by dexamethasone. the ginsenosides $(Rb_1\;Rb_2\;Rc\;Re\;and\;Rg_1)$ and saponin at a concentration of 1 ${\mu}M$ had no significant effect on PMNL chemiluminescence. Thus. the neutral dammarane saponins are potentially important modulators or PMNL function and their inhibitory effects may he differentiated from those of the Steroidal anti-inflammatory agents.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.4
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• pp.344-349
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• 1981

This study was conducted to define the effect of 2.4-D, NAA, Benzyladenine, and basic mediums on the callus induction and the organ differentiation from the meristem tips and the stem and leaf segments of the potato. Benzyladenine promoted the induction and growth of shoot from the meristem tip of potato but inhibited initiation of roots and induction of callus. At higher concentration of NAA than 0.5 ppm and of 2.4-D than 1.0 ppm the shoots were not initiated but the callus was induced from the meristem. The callus growth was significantly promoted on the medium containing NAA than 2.4-0. The initiation and growth of the shoots from the potato meristem was significantly increased in the medium containing 2.4-D and BA, or NAA and BA, compared with those containing BA, NAA or 2.4-D alone. The callus was more easily induced from the stem segments than the leaf segments of potato. And the 2.4-D was more effective for the induction and growth of the callus than the NAA. MS medium diluted its concentration to 1/2 was more suitable for the initiation and growth of the shoots from the potato meristem than the MS standard medium. For the initiation and growth of the shoots from the potato meristem, the most desirable medium was the diluted MS medium containing 1.0 ppm BA and 0.1 ppm NAA or 0.1 ppm 2.4-D.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.3
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• pp.169-175
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• 1985

This experiment was carried out during 1983-1984 to determine an effective method for the introduction of pasture species on nonarable hill country without cultivation. In this experiment, 1.8 and 2.8kg a.i. $ha^{-1}$ rates of glyphosate and a 0.7kg a.i.$ha^{-1}$ rate of paraquat were applied, and also 30, 45 and 60 days intervals between spraying and sowing were used. Establishment of orchardgrass (Dactylis glomerata) was not affected by the application of herbicides, but that of ladino clover (Trifolium repens) was significantly affected by glyphosate and paraquat. It was found that glyphosate and paraquat have no apparent soil residual activity or pre-emergence effect when sprayed 30 to 60 days before sowing. Dry matter yield of pasture species on the herbicide treatments was significantly higher than that on the unsprayed treatments. The highest yield was obtained from the 1.8kg a.i.$ha^{-1}$ rate of glyphosate 45 days before sowing. Botanical analysis at the final cut showed that the shrubs and native grass of original site were reduced to 5% by glyphosate application, but those on the unsprayed control were still 51%. Crude protein content and in vitro dry matter digestibility of herbage on the herbicide treatments were higher than those on the unsprayed treatments. Results indicated that glyphosate was more effective to control resident shrubs and grass than paraquat, and good establishment and higher yield of surface-sown pasture species can be obtained by spraying 1.8 to 2.8kg a.i.$ha^{-1}$ of glyphosate, 30 to 45 days before sowing.

• Korean Journal of Food Science and Technology
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• v.52 no.2
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• pp.149-155
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• 2020

This study was conducted to investigate the potential prebiotic activity of an extract from yacon tuberous root on the growth of probiotic strains Bifidobacterium and Lactobacillus. Results showed that the amount of fructooligosaccharides per 100 g fresh root was the highest (7.60 g), followed by sucrose (0.72 g), fructose (0.34 g), and glucose (0.26 g). The in vitro culture test of intestinal beneficial bacteria, including Bifidobacterium longum BORI, B. bifidum BGN4, and B. lactis AD011 showed effective growth on the MRS-Yacon medium containing yacon extract, whereas the growth of Lactobacillus acidophilus AD031, L. plantarum BH02, and L. fermentum BH03 did not differ from that of the control groups. In particular, B. longum BORI showed better growth than the control group after 10 h of incubation. These results indicate that yacon can be a natural prebiotic source of fructooligosaccharides, which can exert a prebiotic effect on intestinal microflora by selectively enriching Bifidobacterium.

• Korean Journal of Food Science and Technology
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• v.34 no.3
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• pp.510-517
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• 2002

Effects of wheat arabinoxylan on mouse spleen lymphocytes and peritoneal macrophages were examined in vitro. Among three wheat arabinoxylans (A1: low MW, A2: medium MW, A3: high MW), A3$(50{\sim}100\;{\mu}g/mL)$ increased the viability of spleen lymphocytes up to $114{\sim}125%$ of the control. A1 and A3 $(20\;{\mu}g/mL)$ increased the viability of lipopolysaccharide-treated lymphocytes synergistically. Viability of murine peritoneal macrophages treated with wheat arabinoxylans $(10{\sim}100{\mu}g/mL)$ was increased up to $135{\sim}175%$ of the control. The cytotoxic activity of macrophages against murine lymphocytic leukemic cell increased in the presence of wheat arabinoxylan. Phagocytic index of macrophages treated with wheat arabinozylans $(20\;{\mu}g/mL)$ significantly increased $197{\sim}232%$ compared with the control, and lysosomal phosphatase and myeloperoxidase activities also increased significantly (p<0.05). Treatment of wheat arabinoxylans tended to decrease nitrite production, but significantly stimulated $H_2O_2\;and\;O_2$ productions of macrophages (p<0.05). These results indicate that the immunostimulating effect of wheat arabinoxylan may be closely related with lysosomal enzyme activity and reactive oxygen intermediate production of macrophages.

• Journal of dental hygiene science
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• v.9 no.5
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• pp.525-530
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• 2009

The purpose of this study was to evaluate color change and enamel surface changes using the plasma arc light source during tooth bleaching treatments. Twenty-four extracted bovine incisors were selected and embedded in the resin blocks. All the specimens were highly polished and discolored with commercial $COCK^{(R)}$. High concentration carbamide peroxide with and without plasma arc were used for bleaching. Specimens were bleached for 1 hour per week during 3 weeks. Color and enamel surface changes were determined with colorimeter (TC-8600A), microhardness tester(MXT-a7), scanning electron microscope(S-4200). All the collected data analyzed with paired t-test, t-test and one-way ANOVA. After the bleaching, both groups showed the color changes(${\Delta}E^*$). Microhardness of two group decreased after tooth bleaching. The SEM evaluation of enamel surface of both group showed a similar morphology of decalcification after tooth bleaching. Office bleaching using the plasma arc application with 35% carbamide peroxide can increase the color change. Office bleaching using the high concentration of carbamide peroxide and plasma arc also detract the outer surface of enamel. It is recommended that careful procedures are needed during office bleaching with high concentration of carbamide peroxide and light source.

• Korean Journal of Food Science and Technology
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• v.53 no.5
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• pp.544-552
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• 2021

This study investigated the anti-fibrotic and antioxidant effects of Paeonia Radix Alba water extract (PR) on thioacetamide (TAA)-induced liver fibrosis in a mouse model and its underlying mechanisms. Liver fibrosis was induced by intraperitoneal injection of TAA (three times a week) for 8 weeks. Furthermore, silymarin (50 mg/kg body weight) and PR (200 mg/kg body weight) were administered for 8 weeks. PR treatment downregulated aspartate aminotransferase (AST), alanine aminotransferase (ALT), ammonia, and myeloperoxidase levels. Moreover, PR treatment downregulated NOX2 and p47phox and upregulated antioxidant enzymes by activating the Nrf2/Keap1 signaling pathway. Furthermore, PR inhibited the factors associated with fibrosis, such as α-SMA and collagen I. AMPK/SIRT1 was upregulated by PR treatment. Overall, these results suggest that PR attenuates liver fibrosis by regulating the Nrf2/Keap1 and AMPK/SIRT1/NF-κB signaling pathways through the inhibition of oxidative stress. Hence, PR has potential as a remedy for preventing and treating liver fibrosis.