Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Potential prebiotic effects of yacon extract, a source of fructooligosaccharides, on Bifidobacterium strains

프락토올리고당을 함유한 야콘 추출물의 비피더스균에 대한 잠재적인 프리바이오틱 효과

  • Kim, Su Jeong (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Sohn, Hwang Bae (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Kwon, Bin (Research Center, BIFIDO Co., Ltd.) ;
  • Park, Myeong Soo (Research Center, BIFIDO Co., Ltd.) ;
  • Hong, Su Young (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Nam, Jeong Hwan (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Suh, Jong Taek (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Jong Nam (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Ki Deog (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Chang, Dong Chil (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Yul Ho (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
  • 김수정 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 손황배 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 권빈 (비피도(주) 연구소) ;
  • 박명수 (비피도(주) 연구소) ;
  • 홍수영 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 남정환 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 서종택 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 이종남 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 김기덕 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 장동칠 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 김율호 (농촌진흥청 국립식량과학원 고령지농업연구소)
  • Received : 2020.03.06
  • Accepted : 2020.04.02
  • Published : 2020.04.30
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Abstract

This study was conducted to investigate the potential prebiotic activity of an extract from yacon tuberous root on the growth of probiotic strains Bifidobacterium and Lactobacillus. Results showed that the amount of fructooligosaccharides per 100 g fresh root was the highest (7.60 g), followed by sucrose (0.72 g), fructose (0.34 g), and glucose (0.26 g). The in vitro culture test of intestinal beneficial bacteria, including Bifidobacterium longum BORI, B. bifidum BGN4, and B. lactis AD011 showed effective growth on the MRS-Yacon medium containing yacon extract, whereas the growth of Lactobacillus acidophilus AD031, L. plantarum BH02, and L. fermentum BH03 did not differ from that of the control groups. In particular, B. longum BORI showed better growth than the control group after 10 h of incubation. These results indicate that yacon can be a natural prebiotic source of fructooligosaccharides, which can exert a prebiotic effect on intestinal microflora by selectively enriching Bifidobacterium.

본 연구에서는 야콘 덩이뿌리 추출물이 프로바이오틱스인 비피더스균과 락토바실러스균의 생장에 미치는 영향을 구명하고자 하였다. 야콘의 당함량을 HPLC로 분석한 결과, 생체 중 100 g 당프락토올리고당 함량이 7.60 g으로 가장 많았으며, 유리당은 수크로스가 0.72 g, 프럭토스 0.34 g, 글루코스 0.26 g 순의 함량을 보였다. 야콘 추출물이 장내 유산균 활성에 미치는 영향을 구명하기 위해 락토바실러스 3종(L. acidophilus AD031, L. plantarum BH02 and L. fermentum BH03)과 비피더스 3종(B. bifidum BGN4, B. longum BORI, B. lactis AD011) 등 6종을 대상으로 대조군과 야콘처리군 배지에서 유산균의 생장속도를 비교하였다. 락토바실러스 3종의 균주들은 대조군과 야콘처리군에서 대수기에 도달하는 시간이 비슷하였고, 생균수에서도 비슷하거나 감소하는 경향을 보였다. 하지만, 비피더스 3종의 경우 야콘처리군에서 모두 생장이 증가되었고, 특히, B. longum BORI 균주는 배양 10시간 이후부터 대조군과의 확연한 차이를 나타냈다. 이와 같은 결과로, 야콘 추출물은 장내 유익균 중에서도 비피더스균에 특이적인 증식 효과를 보이며, 야콘의 프락토올리고당이 비피더스균 증식에 적합한 영양원임을 확인하였다.

Keywords

References

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