• Food Science and Biotechnology
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• v.17 no.5
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• pp.1032-1037
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• 2008

Lowly volatile heterocyclic sulfur compounds generated in autoclaved garlic extract were isolated and identified, and their antimicrobial activity was determined. Two kinds of unusual volatile sulfur compounds were separated from heated garlic by preparative recycling high performance liquid chromatography (HPLC), and identified by gas chromatography (GC)-mass spectrometry (MS) and $^1H$-nuclear magnetic resonance (NMR). They had heterocyclic structures with 4 to 5 sulfur atoms in the molecules. 4-Methyl-1,2,3-trithiolane (MTTT) is highly volatile and was not able to be concentrated, and was identified by GCMS only. MTTT and 6-methyl-1,2,3,4,5-pentathiepane (MPTP) are lowly volatile and were obtained in pure states to be positively identified for the first time. All 3 heterocyclic sulfur compounds began to appear by the time when the early-formed diallyl sulfides started to disappear. The minimum inhibitory concentration range of MTTT and MPTP was determined to be between 1 and 6 ppm against all yeasts tested. MTTT and MPTP were lowly volatile and sparingly soluble in water.

• Parasites, Hosts and Diseases
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• v.52 no.5
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• pp.565-568
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• 2014

Ticks and tick-borne diseases are important in human and livestock health worldwide. In November 2012, ixodid ticks were collected and identified morphologically from cattle and wild animals in the Maswa district and Iringa urban, Tanzania. Amblyomma gemma, A. lepidum, and A. variegatum were identified from Maswa cattle, and A. variegatum was the predominant species. A. marmoreum, Hyalomma impeltatum, and Rhipicephalus pulchellus were identified from Iringa cattle in addition to the above 3 Amblyomma species, and A. gemma was the most abundant species. Total 4 Amblyomma and 6 Rhipicephalus species were identified from wild animals of the 2 areas. A. lepidum was predominant in Maswa buffaloes, whereas A. gemma was predominant in Iringa buffaloes. Overall, A. variegatum in cattle was predominant in the Maswa district and A. gemma was predominant in Iringa, Tanzania.

• Korean Journal of Veterinary Research
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• v.54 no.4
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• pp.219-224
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• 2014

Uterine sterilization is important for improving fertility in cattle. This study compared bacterial flora in the uterus between healthy and repeat breeder cows (RBCs). The uterine flushing of six heifers, 13 healthy HanWoo cows and eight RBCs (HanWoo) were sampled, and 15 frozen semen samples were selected. Overall, 35 bacteria were identified from in HanWoo uterine flushing and semen. The bacterial genera identified from HanWoo uterine flushing were Alloiococcus, Bacillus, Enterobacter, Enterococcus, Erysipelothrix, Gardnerella, Granulicatella, Kocuria, Pantoea, Pasteurella, Rothia, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The bacterial genera identified from HanWoo semen were Bacillus, Escherichia, Kocuria, Oligella, Pseudomonas, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The prevalence and presence of the identified bacteria between healthy cows and RBCs differed significantly. Further studies are needed to determine the role of these bacteria in the uterus of HanWoo cattle with reproductive disorder.

• Journal of Microbiology and Biotechnology
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• v.18 no.9
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• pp.1510-1517
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• 2008

Expresssed sequence tag (EST) analysis was developed from three cDNA libraries constructed from cells of the digestive tract, gonad, and liver of sea squirt. Randomly selected cDNA clones were partially sequenced to generate a total of 922 ESTs, in which 687 unique ESTs were identified respectively. Results of BLASTX search showed that 612 ESTs (89%) have homology to genes of known function whereas 75 ESTs (11%) were unidentified or novel. Based on the major function of their encoded proteins, the identified clones were classified into ten broad categories. We also identified several kinds of immune-related genes as identifying novel genes. Sequence analysis of ESTs revealed the presence of microsatellite-containing genes that may be valuable for further gene mapping studies. The accumulation of a large number of identified cDNA clones is invaluable for the study of sea squirt genetics and developmental biology. Further studies using cDNA microarrays are needed to identify the differentially expressed transcripts after disease infection.

• The Korean Journal of Food And Nutrition
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• v.11 no.5
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• pp.499-505
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• 1998

The purpose of this study was to ivestigate the structural characteristics of waste soulble browning reaction prodcuts (WS-BRPs) isolated from Korean red ginseng. They all showed the maximum absorbances at near 280 nm. Their IR spectra suggested the presence of hydroxyl, amide carbonyl and aliphatic methine groups. From sugar analysis it was identified that L and S-1 contained two kinds of suggar, glucose and xylose, and S-2, a fructose together with glucose and xylose. Thirteen different amino acids were identified in L. Ten amino acids from S-1 and seven amino acids from S-2 were identified using Auto Amino Acid Analyzer. Glycine, serine and glutamic acid in S-1 and one unknown amino acid and glycine in S-2 were detected as the major amino acids, respectively. From the 1H-and 13C-NMR spectra, it was identified that a number of sugar moieties, carbonyl and carbon double bonds (only in S-2) were contained in the three WS-BRP components. Approximate number of sugar moiety of L, S-1 and S-2 was determined to be 8∼10, 9∼11 and 4∼5, respectively. Contents of nitrogen, carbon and hydrogen showed L>S-1>S-2.

• Journal of the Korean Society of Costume
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• v.55 no.8 s.99
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• pp.112-126
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• 2005

The purpose of this study is to analyze the aesthetic characteristics of 'movement' expression in modern fashion(1910-2004) based on a study of modern fine arts which adopted 'movement' element in their work. In this study the meaning of movement was defined as motion, changing position and transformation. Literature survey through books and research papers and demonstrative study with fashion collection photos were undertaken. The results wert as follows ; 1) Kinetic art, optical an, light kinetic art and technology art such as video and computer art have adopted 'movement' element in their work. 2) The plasticities of 'movement' fine arts were identified as mutual penetration, increase of visibility, use of non-traditional materials and dynamism. The internal meanings were identified as expansion of aesthetic experience and the concept of fine art, optimistic attitude on technology, spectator participation and integration of art and life. 3) The 'movement' expression in modern fashion was distinctively found in 1910s-20s(avant-garde fashion), 1960s (kinetic and optical art fashion) and mid 1990s to 2004 (techno-cyber fashion). 4) The plasticities of the 'movement' expression in modern fashion were identified as non-definition, use of non-traditional materials, dynamism. The internal meanings were identified as expansion of aesthetic experience and the concept of dress, optimistic attitude on technology, playfulness through participation. In conclusion, the expression of 'movement' in modern fashion has optimistic viewpoint on the development of modern society and is one of the interesting design points which will be pursued in the fellowing years.

• Fisheries and Aquatic Sciences
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• v.17 no.2
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• pp.215-222
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• 2014

To explore marine microorganisms with medical potential, we isolated and identified marine bacteria from floats, marine algae, animals, and sponges collected from Jeju Island, Korea. We isolated and identified 21 different strains from the marine samples by 16S rRNA analysis, cultured them in marine broth, and extracted them with ethyl acetate (EtOAc) to collect secondary metabolite fractions. Next, we evaluated their anti-oxidative and anti-inflammatory effects. Among the 21 strains, the secondary metabolite fraction of Bacillus badius had both strong antioxidant and anti-inflammatory activity, and thus was selected for further experiments. An antioxidant compound detected from the secondary metabolite fraction of B. badius was purified by preparative centrifugal partition chromatography (n-hexane:EtOAc:methanol:water, 4:6:4:6, v/v), and identified as diolmycin A2. Additionally, diolmycin A2 strongly inhibited nitric oxide production. Thus, we successfully identified a significant bioactive compound from B. badius among the bacterial strains collected from Jeju Island.

• The Korea Journal of Herbology
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• v.21 no.2
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• pp.129-133
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• 2006

Objectives : Cervus elaphus species are some of the most medicinally important genera in the Oriental medicine. This study was performed to determine if Cenvus elaphus species could be identified by sequencing analysis and to verify Basic Local Alignment Search Tool (BLAST) search, which was used to assess genetic identification. Methods : The DNAs of Cervus elaphus species were extracted, amplified by PCR, and sequenced. The DNAs of Cervus species were identified by BLAST search in website. Results : By BLAST search one of Cervus elaphus species was identified as Cervus elaphussibericus but the other was identified as Cervus elaphus nelsoni. This work showed that identification can efficiently be performed by BLAST search. Conclusion : These results suggest that sequencing following BLAST search might be able to provide the identification of Cervus elaphus species.

• International Journal of Fuzzy Logic and Intelligent Systems
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• v.5 no.1
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• pp.76-82
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• 2005

Speech signal has various features of speakers. This feature is extracted from speech signal processing. The speaker is identified by the speaker identification system. In this paper, we propose the speaker identification system that uses the incremental learning based on neural network. Recorded speech signal through the microphone is blocked to the frame of 1024 speech samples. Energy is divided speech signal to voiced signal and unvoiced signal. The extracted 12 orders LPC cpestrum coefficients are used with input data for neural network. The speakers are identified with the speaker identification system using the neural network. The neural network has the structure of MLP which consists of 12 input nodes, 8 hidden nodes, and 4 output nodes. The number of output node means the identified speakers. The first output node is excited to the first speaker. Incremental learning begins when the new speaker is identified. Incremental learning is the learning algorithm that already learned weights are remembered and only the new weights that are created as adding new speaker are trained. It is learning algorithm that overcomes the fault of neural network. The neural network repeats the learning when the new speaker is entered to it. The architecture of neural network is extended with the number of speakers. Therefore, this system can learn without the restricted number of speakers.

• Journal of Environmental Health Sciences
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• v.10 no.1
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• pp.21-31
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• 1984

The purpose of this study was to find out more effective schemes on safe water supply in rural area. Then, this study was carried out to investigate drinking water qualities of 20 simple piped water supply systems and 20 water pumps in Chun Sung County, Kang Won Province from 12th December to 24th December, 1983 and was compared this results with the previous results that had been carried out at the same sample sites from 1st July to 30th August, 1981. 1. This results of water quality in winter are better than the previous results in summer because this results are identified as safe 20%, unsafe 35% in simple piped water supply systems, safe 50%, unsafe 20% in water pumps and the previous results are identified as safe 1.8%, unsafe 78.6% in simple piped water supply systems, safe 5.5%, unsafe 73.6% in water pumps. 2. Water qualities of water pumps are better than that of simple piped water supply systems because the former is identified as safe 50%, unsafe 20% and the latter is identified as safe 20%, unsafe 35%. 3. Drinking waters that are in need of chlorination in winter as well as summer are 45% of simple piped water supply systems and 30% of water pumps.