The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Identification of Cervus elaphus Species by Sequencing Analysis and BLAST Search

Cervus elaphus 종의 sequencing과 BLAST search에 의한 감별

  • Seo, Jung-Chul (The Research Center for Biomedical Resources of Oriental Medicine, College of Oriental Medicine, Daegu Hanny University) ;
  • Kim, Min-Jung (The Research Center for Biomedical Resources of Oriental Medicine, College of Oriental Medicine, Daegu Hanny University) ;
  • Lee, Chan (The Research Center for Biomedical Resources of Oriental Medicine, College of Oriental Medicine, Daegu Hanny University) ;
  • Leem, Kang-Hyun (Department of Herbal Pharmacology, College of Oriental Medicine, Semyung University)
  • 서정철 (대구한의대학교 한방생명자원연구센터) ;
  • 김민정 (대구한의대학교 한방생명자원연구센터) ;
  • 이찬 (대구한의대학교 한방생명자원연구센터) ;
  • 임강현 (세명대학교 한의과대학 본초학교실)
  • Published : 2006.06.30
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Abstract

Objectives : Cervus elaphus species are some of the most medicinally important genera in the Oriental medicine. This study was performed to determine if Cenvus elaphus species could be identified by sequencing analysis and to verify Basic Local Alignment Search Tool (BLAST) search, which was used to assess genetic identification. Methods : The DNAs of Cervus elaphus species were extracted, amplified by PCR, and sequenced. The DNAs of Cervus species were identified by BLAST search in website. Results : By BLAST search one of Cervus elaphus species was identified as Cervus elaphussibericus but the other was identified as Cervus elaphus nelsoni. This work showed that identification can efficiently be performed by BLAST search. Conclusion : These results suggest that sequencing following BLAST search might be able to provide the identification of Cervus elaphus species.

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