• Ocean and Polar Research
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• 제33권2호
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• pp.159-169
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• 2011

Antifreeze proteins (AFPs) have ice binding affinity, depress freezing temperature and inhibit ice recystallization which protect cellular membranes in polar organisms. Recent structural studies of antifreeze proteins have significantly expanded our understanding of the structure-function relationship and ice crystal growth inhibition. Although AFPs (Type I-IV AFP from fish, insect AFP and Plant AFP) have completely different fold and no sequence homology, they share a common feature of their surface area for ice binding property. The conserved ice-binding sites are relatively flat and hydrophobic. For example, Type I AFP has an amphipathic, single ${\alpha}$-helix and has regularly spaced Thr-Ala residues which make direct interaction with oxygen atoms of ice crystals. Unlike Type I AFP, Type II and III AFP are compact globular proteins that contain a flat ice-binding patch on the surface. Type II and Type III AFP show a remarkable structural similarity with the sugar binding lectin protein and C-terminal domain of sialic acid synthase, respectively. Type IV is assumed to form a four-helix bundle which has sequence similarity with apolipoprotein. The results of our modeling suggest an ice-binding induced structural change of Type IV AFP. Insect AFP has ${\beta}$-helical structure with a regular array of Thr-X-Thr motif. Threonine residues of each Thr-X-Thr motif fit well into the ice crystal lattice and provide a good surface-surface complementarity. This review focuses on the structural characteristics and details of the ice-binding mechanism of antifreeze proteins.

• 대한조선학회논문집
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• 제41권6호
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• pp.75-83
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• 2004

Ice rubble pieces broken by the bow impact load and side hull of an icebreaking vessel usually pass along the ship's bottom hull and may hit the propeller/rudder or other stern structures causing serious damage to ship's hull . Therefore it is important to estimate the size of broken ice pieces during the icebreaking process. The dynamic interaction process of icebreaker with infinite ice sheet is simplified as a wedge type beam of finite length supported by elastic foundation. The wedge type ice beam is leaded with vertical impact forces due to the inclined bow stem of icebreaking vessels. The numerical model provides locations of maximum dynamic bending moment where extreme tensile stress arises and also possible fracture occurs. The model can predict a failure length of broken ice sheet given design parameters. The results are compared to Nevel(1961)'s analytical solution for static load and observed pattern of ice sheet failure onboard an icebreaker. Also by comparing computed failure length with the characteristic length, the meaning of ice rubble sizes is discussed.

• 한국태양에너지학회 논문집
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• 제33권2호
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• pp.50-55
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• 2013

On a plan for the Winter Olympics 2018, Korean government is in the process of the world's first use of ocean energy for the Olympic ice-rink. This technology will be applied to a seaside town and have possibility of an export industry. In this study, we researched facilities and system for P ice-rink that acts as a cultural center as well as a physical plant in Busan and provided the way that apply by seawater heat source. Also, existing system and seawater heat source system of P ice-rink was analyzed by the most commonly used life cycle cost analysis among economics methods. Such economics data for ice-rink using seawater will be utilized by a basic information.

• 설비공학논문집
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• 제11권3호
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• pp.301-312
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• 1999

Experiments were carried out to evaluate performance of the cold air distribution systems with an ice storage tank in test room. Cold air distribution systems provide primary air for comfort conditioning or process cooling at coil discharge temperatures$4^{\circ}C$ to$11^{\circ}C$. The application of a cold air distribution system allows for the downsizing of air distribution equipment and central plant equipment when ice storage tank is used. The benefit of a cold air distribution system include a decrease in the floor-to-floor height, increase floor space, reduced building capital costs, reduced energy use and demand. The use of cold air distribution can result in the most cost effective system and is currently being implemented world wise as the new standard in air conditioning systems. In this study, the cold air distribution system is compared with the general ice storage system. Under the same cooling load conditions, experimental results show that the supply air volume of cold air distribution system decrease 38%, and decrease 45% flow rate of brine for the general ice storage system.

• 식물조직배양학회지
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• 제27권4호
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• pp.339-343
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• 2000

When plants are exposed to subfreezing temperatures ice crystals are forming within extracelluar space in leaves. The growth of ice crystal is closely related to the degree of freezing injury. It was shown that an antifreeze protein binds to an ice nucleator through hydrogen bonds to prevent growth of ice crystal and also reduces freezing damage. The antifreeze proteins in plants are similar to PR proteins but only the PR proteins induced upon cold acclimation were shown to have dual functions in antifreezing as well as antifungal activities. Three of the genes encoded for CLP, GLP, and TLP were isolated from barley and Kentucky bluegrass based on amino acid sequence revealed after purification and low temperature-inducibility as shown in analysis of the protein. The deduced amino acid of the genes cloned showed a signal for secretion into extracellular space where the antifreezing activity sup-posed to work. The western analysis using the antisera raised against the antifreeze proteins showed a positive correlation between the amount of the protein and the level of freeze tolerance among different cultivars of barely. Besides it was revealed that TLP is responsible for a freeze tolerance induced by a treatment of trinexapac ethyl in Kentucky bluegrass. Analysis of an overwintering wild rice, Oryza rufipogon also showed that an acquisition of freeze tolerance relied on accumulation of the protein similar to CLP. The more direct evidence for the role of CLP in freeze tolerance was made with the analysis of the transgenic tobacco showing extracellular accumulation of CLP and enhanced freeze tolerance measured by amount of ion leakage and rate of photosynthetic electron transport upon freezing. These antifreeze proteins genes will be good candidates for transformation into crops such as lettuce and strawberry to develop into the new crops capable of freeze-storage and such as rose and grape to enhance a freeze tolerance for a safe survival during winter.

• 생물환경조절학회지
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• 제25권2호
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• pp.106-110
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• 2016

식물의 생육과 생산성을 예측하는 것은 매우 중요한 일이다. 본 연구는 common ice plant (Mesembryanthemum crystallinum L.)의 작물생장률, 상대생장률, 지상부 생체중과 건물중, 수확시기, 상품률과 상품수량과 같은 생육과 수확 요인들을 쉽게 읽을 수 있는 재식밀도-생육-수확(PGH) 도표를 만들기 위함이다. 광도 $140{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$과 일장 12시간 주기로, 3파장 형광등을 이용한 완전제어형 식물공장 시스템에서 박막수경 재배하였다. 4가지 재식밀도($15{\times}10cm$, $15{\times}15cm$, $15{\times}20cm$, and $15{\times}25cm$) 하에서 생육과 수량을 분석하였다. 재식밀도가 증가할수록 어느 한계까지는 식물체당 생체중과 건물중은 증가하는 경향이었으며, 단위면적당 생체중인 수량 또한 같은 경향을 보였다. 작물생장률, 상대생장률과 lost time은 2차 등식 형태를 보였으며, 지상부 생체중과 건물중은 직선적인 관계를 보였다. 이러한 등식을 이용하여 재식밀도-생육-수확(PGH) 도표를 만들었다. 예를 들면, $15{\times}20cm$ 재식밀도와 식물체당 생체 중 100g에서 수확할 경우, 재식주수, 작물생장률, 상대생장률, lost time, 식물체당 건물중, 수확시기와 수량은 각각 $33plants/m^2$, $20g{\cdot}m^{-2}{\cdot}d^{-1}$, $0.27g{\cdot}g^{-1}{\cdot}d^{-1}$, 22days, 2.5g/plant, 정식 후 26일과 $3.2kg{\cdot}m^{-2}$이었다. 이 도표를 가지고 적어도 2가지 요인 예를 들면, 재식밀도와 수확요인 중 하나만 알면, 작물생장률, 상대생장률, lost time과 같은 생육 요인과 지상부 생체중, 지상부 건물중, 수확시기와 수량과 같은 수확 요인들을 쉽게 구할 수 있다. 이러한 도표는 다양한 작물의 생육과 수량 요인을 예측할 수 있어 완전제어형 식물 생산 시스템 설계를 위해 유용한 도구가 될 것이다.

• 한국표면공학회지
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• 제54권5호
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• pp.222-229
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• 2021

Icing causes various serious problems, where water vapor or water droplets adhere at cold conditions. Therefore, understanding of ice adhesion on solid surface and technology to reduce de-icing force are essential for surface finishing of metallic materials used in extreme environments and aircrafts. In this study, we controlled wettability of aluminum alloy using anodic oxidation, hydrophobic coating and lubricant-impregnation. In addition, surface porosity of anodized oxide layer was controlled to realize superhydrophilicity and superhydrophobicity. Then, de-icing force on these surfaces with a wide range of wettability and mobility of water was measured. The results show that the enhanced wettability of hydrophilic surface causes strong adhesion of ice. The hydrophobic coating on the nanoporous anodic oxide layer reduces the adhesion of ice, but the volume expansion of water during the freezing diminishes the effect. The lubricant-impregnated surface shows an extremely low adhesion of ice, since the lubricant inhibits the direct contact between ice and solid surface.

• The Plant Pathology Journal
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• 제38권4호
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• pp.403-409
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• 2022

In biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to determine the INA of the strains, using Pseudomonas syringae pv. syringae KACC 21200 as a positive control and resulting in seven INA+ fungal strains that were identified as F. tricinctum (KNUF-21-F17, KNUF-21-F18, KNUF-21-F29, KNUF-21-F32, KNUF-21-F38, KNUF-21-F43, and KNUF-21-F44). The effect of Fusarium INA+ KNUF-21-F29 was compared to that of INA- strains on Chrysanthemum morifolium cv. Shinma explants. A higher callus formation and no-shoot formation were observed, suggesting that fungal INA could play a role in cold injuries and be a factor to consider in rapid apple decline. To the best of our knowledge, this is the first report of INA fungal strains isolated in Korea.

• 대한조선학회논문집
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• 제55권6호
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• pp.497-504
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• 2018

Platform Support Vessels operated in the Arctic Ocean support diverse operations of offshore plant in the sea, and the PSV is also needed to support works to exploit the oil and gas in the Arctic Ocean. Both of the ice breaking and the open sea performance have been considered together to secure the enhanced operational performance at the harsh environment in the Arctic Ocean and the open sea as well. In this study, One of the design requirements of a PSV is to guarantee continuous icebreaking performance with 3 knots at 1 m thickness of level ice, where the design draft is 7.5m and the engine power is 13 MW. Three hull forms were designed, and the ice resistance based on empirical formulas was estimated to select the initial hull form having an outstanding performance. The full scale performance of the designed hull forms was predicted by the ice model test conducted in the ice model basin of Korea Research Institute of Ships & Ocean Engineering(KRISO). The analysed results show that the selected hull form satisfies the above design requirement.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2016년도 추계학술대회
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• pp.230-233
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• 2016

본 논문에서는 원격지에서 제빙기의 상태를 확인할 수 있는 제빙기 상태 모니터링 시스템을 개발하였다. 개발된 시스템은 제빙기에 연결되어 전류, 전압, 냉매압력, 냉매온도 등의 데이터를 주기적으로 서버에게 송신하는 데이터 수집용 통신보드와 이 보드로부터 전송된 데이터를 데이터베이스에 저장하는 서버 프로그램, 데이터베이스에 저장된 데이터를 관리자 및 장비 운용자에게 보여주는 웹 기반의 사용자 응용프로그램으로 구성된다. 제빙기에 이상 징후가 감지되면, 이 시스템을 통해 얻은 데이터를 활용하여 관리자와 운용자는 제빙기의 상태를 실시간으로 확인하고 적절한 조치를 취함으로써 장비의 고장을 예방할 수 있다.