The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Isolation and Identification of Ice Nucleation Active Fusarium Strains from Rapid Apple Declined Trees in Korea

  • Avalos-Ruiz, Diane (School of Applied Biosciences, Kyungpook National University) ;
  • Ten, Leonid N. (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Chang-Kil (Department of Horticultural Science, Kyungpook National University) ;
  • Lee, Seung-Yeol (School of Applied Biosciences, Kyungpook National University) ;
  • Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University)
  • Received : 2022.04.05
  • Accepted : 2022.06.02
  • Published : 2022.08.01
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Abstract

In biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to determine the INA of the strains, using Pseudomonas syringae pv. syringae KACC 21200 as a positive control and resulting in seven INA+ fungal strains that were identified as F. tricinctum (KNUF-21-F17, KNUF-21-F18, KNUF-21-F29, KNUF-21-F32, KNUF-21-F38, KNUF-21-F43, and KNUF-21-F44). The effect of Fusarium INA+ KNUF-21-F29 was compared to that of INA- strains on Chrysanthemum morifolium cv. Shinma explants. A higher callus formation and no-shoot formation were observed, suggesting that fungal INA could play a role in cold injuries and be a factor to consider in rapid apple decline. To the best of our knowledge, this is the first report of INA fungal strains isolated in Korea.

Keywords

Acknowledgement

This work was carried out with the support of the Cooperative Research Program for Agriculture, Science and Technology Development (Project No. PJ014998022022), Rural Development Administration, Republic of Korea.

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