• FLOWER RESEARCH JOURNAL
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• v.17 no.3
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• pp.165-171
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• 2009

This experiment was conducted to extend utility of in-vitro culture material stalk and to mass produce uniform plants in Phalaenopsis. The optimal concentration of hyponex medium and plant hormone were examined. The most effective concentration of the hyponex in the flower stalk tissue culture of Phalaenopsis was $4mg{\cdot}L^{-1}$. The most effective concentration of TDZ and BA on the formation of multiple shoots in Phalaenopsis flower stalk culture was $0.3mg{\cdot}L^{-1}$ and $5mg{\cdot}L^{-1}$, respectively. TDZ was more effective for formation of the shoots and multiple shoots than BA at the basic medium with hyponex $4g{\cdot}L^{-1}$.

• FLOWER RESEARCH JOURNAL
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• v.17 no.2
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• pp.114-120
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• 2009

The most effective conditions of in vitro culture were studied for mass propagation of Pteris cretica 'Wilsonii'. Spores of the species germinated within 7 weeks. The greatest proliferation was obtained with Knop and Hyponex media, but growth was more effective in Hyponex medium. MS medium induced necrosis of prothalli in all strength of nitrogen and sucrose except in case of 0% sucrose. Hyponex medium supplemented with 1% sucrose and 0.6% agar promoted propagation and growth of prothalli. In Hyponex medium, optimal inoculation method was homogenization, but in MS medium dividing colonies of prothalli was more effective. Culturing on solid medium was more effective than liquid culture method. Liquid culture induced necrosis of prothalli. Shaking cultured prothalli showed good growth, but propagation was inhibited compared to those cultured on solid medium.

• Korean Journal of Plant Tissue Culture
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• v.25 no.3
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• pp.189-194
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• 1998

The experiment was tried to identify culture media which was suitable for seed germination and seedling growth of wild orchids, Calanthe discolor and Habenaria radiata. When seeds of Calanthe discolor, which was treated with ultrasonics for 30 minutes, were sowed in Murashige and SKoog(MS) medium, germination was much more promoted than other treatments. Seedling of C. discolor grew more rapidly in 3g/L Hyponex and 2g/L tryptone(H$_3$T$_2$) medium and 3g/L Hyponex and 2g/L peptone(H$_3$P$_2$) medium, especially in H$_3$P$_4$ medium among those media, pseudobulb became more corpulent. Habenaria radiata, whose tubers were obtained from seedlings, were sprouted more vigorously in 3g/L Hyponex and 1g/L peptone(H$_3$P$_1$) medium and 1g/L Hyponex and 2g/L peptone(H$_1$P$_2$) medium; but multiplication of tubers, growth of daughter tubers and its corpulence were well established in 1g/L Hyponex and 2g/L peptone(H$_1$P$_2$) medium.

• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.9-14
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• 2009

This study was conducted to find out the commercial in vitro propagation methods through protocom-like bodies (PLB) induced from shoot tip culture of Miltonia spp. Among several culture media for induction PLB from shoot tip in Miltonia spp., MS basal medium was better than Hyponex, Vacin & Went basal medium and other media supplemented with natural additives. PLB's proliferation and differentiation in Hyponex medium including $20g{\cdot}L^{-1}$ banana + $20g{\cdot}L^{-1}$ sucrose(pH 5.2) was better than in MS medium. It was tendency that solid media showed higher PLB fresh weight than liquid medium or other cotton bridge culture. The dark culture for 1~2 weeks and adding $10{\sim}20g{\cdot}L^{-1}$ sucrose on hyponex basal medium was the most effective to increase the PLBs growth and shoot number.

• Journal of the Korean Institute of Landscape Architecture
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• v.29 no.4
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• pp.82-90
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• 2001

This study was carried out to reveal optimum conditions for in vitro propagation of 3 Korean native azalea species, Rhododendron mucronulatum, R. yedonese var. poukhanense, and R. shlippenbachii, which are useful for landscape proposes. Seeds and meristems from three azalea species were cultured on 1/2MS, Hyponex, and Anderson media containing growth of regulators benzyladenine(BA) and 2-isopentenyadenosine(2ip). The results were as follows. 1. In the culture of R. schlippenbachii and R. mucronulatum seeds, in vitro seedlings germinated and grew well on he 1/2MS and Anderson media, while R. yedoense var. poukhanense on Hyponex media containing 6.0mg/$\ell$ 2ip. 2. When the meristems of R. mucronulatum were cultured on Andeson media containing 9.0mg/$\ell$ 2ip, the survival rate of meristems was 23.0% in 6 weeks after culture, and the survival rate of R. schlippenbachii was 46.0% o nthe same media containing 12.0mg/$\ell$ 2ip. The survial rate of R. yedoense var. poukhanense was 92.0% onHyponex media containing 0.5mg/$\ell$ BA and 9.0mg/$\ell$ 2ip. When the meristems of R. mucronulatum and R. yedoense var. poukhanense were cultured on Hyponex media containing 12.0mg/$\ell$ 2ip, they showed the most excellent growth. R. schlippenbachii grew well on Anderson media containing 9.0mg/$\ell$ 2ip. When in vitro shoots of R. yedoense var. poukhanense were subcultured to solid medium, they grew well in shoot growth on Hyponex media containing 6.0mg/$\ell$ 2ip.

• Current Research on Agriculture and Life Sciences
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• v.3
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• pp.1-7
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• 1985

The study was conducted in order to get basic information on seed germination and seedling growth through the experiments such as basal medium, concns of auxin, sucrose agar and pH values. Seed germination was very excellent in $3g/{\ell}$ hyponex and $5g/{\ell}$ peptone containing medium. Multiple shoots were formed in $1-3g/{\ell}$ hyponex and $5g/{\ell}$ pepton containg medium supplemented with $1.0g/{\ell}$ IBA, $30g/{\ell}$ sucrose and $8g/{\ell}$ agar. Growth of seedlings was more stimulated in the same amount of hyponex and peptone as above medium substituted with $40g/{\ell}$ sucrose and $6g/{\ell}$ agar without IBA.

• Korean Journal of Plant Resources
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• v.1 no.1
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• pp.88-92
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• 1988

The study was conducted to determine the optimal basal media and the concentration of plant growth regulator for germination of seeds and growth of plantlet from Dendrobium monile. The results obtained were summarized as follows. Germination was similar in light and dark condition, but the growth of plantlet after germination was better under dark than under light condition in several media. Germination was best in Hyponex and Kyoto solution medium among the 9 media tested. The number of roots/shoot was most in the Hyponex medium containing 0,1ppm NAA and 1.Oppm BA.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.246-250
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• 2009

This study was conducted to determine the effect of controlled-release fertilizer on growth of Oncidium. Leaf and pseudobulb length increased with controlled-release fertilizer from 2 g to 3 g treatment compared to hyponex and controlled-release fertilizer I g treatment, while leaf width and number of leaf was not significant among treatments. Plant weight increased with controlled-release fertilizer from 2 g to 3 g treatment compared to hyponex and controlled-release fertilizer I g treatment. Flowering date(bloomed October early) did not show significant difference among treatments. The number of flowers showed the most in controlled-release fertilizer 3 g treatment as 62.4 ea/plant, but was not different significantly among treatments. Flower stem length and width were also non-significant among treatments. Branching numbers increased in the controlled-release fertilizer 2 g and 3 g treatments. Mineral elements of shoot increased from 2 g to 3 g treatment rather than the controlled-release fertilizer 1g treatment. Especially, K content was higher compared to N and P content. Therefore, appropriate controlled-release fertilizer amount for Oncidium was recommended as 2 g.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.50-50
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• 2018

최근 생태 조경용 관상식물의 수요가 증가함에 따라, 환경적응성이 강하며 관상용으로 가치가 있는 자생식물들을 개발하기 위한 노력이 증가하고 있다. 둥근산꼬리풀(Veronica rotunda Nakai)은 화기가 길며, 꽃이 만개하였을 때 관상가치가 높아 정원의 지피식물 및 실내 분화식물로 이용 가치가 높다. 본 연구는 둥근산꼬리풀의 육묘기술을 개발하기 위하여 2018년 5월 8일에서 6월 30일까지 약 8주간 시행되었다. 본 연구의 공통사항은 200구 트레이에 원예상토를 충진한 후 셀당 4립씩 종자를 파종하였다. 이후 무차광 조건에서 육묘하였으며, 추비는 hyponex $500mg{\cdot}L^{-1}$를 잎이 충분히 젖도록 2, 4주차에 엽면시비 하였다. 파종 용기별 실험은 162, 200, 288구 트레이를 사용하였고, 파종립수별 생육을 알아보기 위하여 200구 트레이에 셀 당 1, 2, 4 및 6립을 파종하였다. 토양종류는 원예상토, 피트모스:펄라이트(3:1, 4:1)의 혼용토를 육묘에 이용하였다. 각 혼용토의 비료 농도는 한아름상토(N:P:K, $300-200-200mg{\cdot}L^{-1}$)를 기준으로 0, 100 및 200%로 조성하였다. 추비는 hyponex (6.5-6-19)를 물에 희석하여 0, 500, 및 $1000mg{\cdot}L^{-1}$로 파종 후 2, 4주차 총 2회 잎이 충분히 젖을 정도로 엽면시비 하였으며, 차광실험은 0, 55 및 75% 차광막이 설치된 곳에 재배하였다. 8주간 육묘 실험을 수행한 결과, 파종 용기별로는 162구 트레이에서 생육한 유묘에서 각 측정 항목별로 유의적으로 양호한 결과가 나타났다. 셀 당 파종립수 별로는 1립 파종구에서 가장 우수한 유묘의 생장을 보였으며, 파종립수가 증가할수록 생육이 감소하는 경향이 나타났다. 토양조성을 달리하였을 때 원예상토 단용 처리에서 가장 생육이 우수하였고, 피트모스가 혼합된 토양에서는 생육이 저조한 경향을 보였다. Hyponex의 농도를 달리하여 추비하였을 때 $1000mg{\cdot}L^{-1}$에서 유묘의 생육이 가장 양호하였으며, 추비농도가 감소할수록 생육이 감소하는 경향을 보였다. 차광정도에 따른 생육을 관찰한 결과 55% 차광에서 생육이 가장 우수하였고, 75% 차광의 경우에는 무차광 처리구에 비해 생육이 감소하는 경향이었다. 따라서 각 처리별 결과를 토대로 둥근산꼬리풀을 육묘시 162구 트레이에 원예상토를 충진한 후 셀 당 1립 파종하여, 55% 차광에서 재배하며, 추비는 Hyponex $1000mg{\cdot}L^{-1}$를 잎이 충분히 젖을 정도로 엽면시비하는 것이 가장 효과적일 것으로 생각된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.62-62
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• 2018

산꼬리풀(Veronica rotunda var. subintegra T.Yamaz.)은 다년생 초본류로 달성이라는 꽃말을 가진 우리나라에만 자생하는 특산식물이다. 줄기는 곧게 서며, 줄기 끝에 보라색의 꽃이 총상화서로 피어 관상가치도 우수하다. 또한, 전초를 일지향이라 불리우며, 중약대사전에는 천식과 기침, 감기, 기관지염 등에 효능이 있다고 보고되었다. 이러한 산꼬리풀의 육묘 체계의 확립을 위하여 2017년 6월 2일부터 7월 31일까지 약 8주간 육묘하였다. 종자 육묘기술 개발을 위한 공통조건은 200구 트레이에 원예상토를 충진한 다음 셀 당 4립씩 파종하여 55% 차광막이 설치된 배드에 배치하였으며, 이후 4, 6주차에 Hyponex $500mg{\cdot}L^{-1}$을 처리하였다. 토양실험은 원예상토 단용[$300-200-200mg{\cdot}L^{-1}$(N-P-K)], 피트모스:펄라이트(3:1) 및 코코피트:펄라이트(3:1) 혼용토를 사용하였다. 피트모스와 코코피트의 혼용토는 원예상토의 비료농도를 기준으로 0-0-0, 300-200-200, $600-400-400mg{\cdot}L^{-1}$의 비료를 첨가하였다. 파종 립수별 실험은 셀탕 1, 2, 4 및 6립으로 하였으며, 추비는 hyponex(6.5-6-19) 0, 500, $1000mg{\cdot}L^{-1}$를 증류수에 희석하여 2회 분무기로 살포하였다. 차광 실험은 0, 55, 75% 등의 차광막이 설치된 곳에서 수행하였다. 연구의 결과, 산꼬리풀의 육묘는 원예상토를 사용하는 것이 다른 토양에 비해 우수한 결과를 보였다. 초장, 경직경 및 엽수는 원예상토를 이용하는 것이 가장 효과적이었으며, 다음으로 피트모스:펄라이트 혼용토 순이었다. 근장의 경우, 피트모스 혼용토에서 비료 농도와 관계없이 원예상토에 비해 유의적으로 길었다. 파종립수에 따른 초장과 경직경은 1, 2립 파종구에서 가장 양호하였으며, 파종량이 적을수록 길고 굵어지는 경향이었다. 엽수도 셀 당 파종량이 적을수록 많아지는 경향이었으나, 유의적인 큰 차이는 확인되지 않았다. 추비는 지상부의 경우 hyponex $500mg{\cdot}L^{-1}$ 처리 시 생육의 증가 효과가 있었지만, 비교적 고농도인 $1000mg{\cdot}L^{-1}$ 처리구에서는 생육에 효과가 없거나 억제되는 결과를 보였다. 한편, 근장은 처리농도가 높을수록 증가하는 경향이었고, hyponex $1000mg{\cdot}L^{-1}$ 처리구(9.9 cm)에서 가장 길었다. 산꼬리풀의 유묘 재배 시 차광처리는 경직경과 근장을 감소시키는 결과를 보였으며, 초장과 엽수는 처리에 따른 유의적인 차이는 없었다.