Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Effect of culture Media on Asymbiotic Seed Germination and Those Seedling Growth of Calanthe discolor and Habenaria radiata

새우난초와 해오라비난초 종자의 기내발아와 유묘생장에 미치는 배지의 영향

  • Published : 1998.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

The experiment was tried to identify culture media which was suitable for seed germination and seedling growth of wild orchids, Calanthe discolor and Habenaria radiata. When seeds of Calanthe discolor, which was treated with ultrasonics for 30 minutes, were sowed in Murashige and SKoog(MS) medium, germination was much more promoted than other treatments. Seedling of C. discolor grew more rapidly in 3g/L Hyponex and 2g/L tryptone(H$_3$T$_2$) medium and 3g/L Hyponex and 2g/L peptone(H$_3$P$_2$) medium, especially in H$_3$P$_4$ medium among those media, pseudobulb became more corpulent. Habenaria radiata, whose tubers were obtained from seedlings, were sprouted more vigorously in 3g/L Hyponex and 1g/L peptone(H$_3$P$_1$) medium and 1g/L Hyponex and 2g/L peptone(H$_1$P$_2$) medium; but multiplication of tubers, growth of daughter tubers and its corpulence were well established in 1g/L Hyponex and 2g/L peptone(H$_1$P$_2$) medium.

우리나라 야생란 중 원예적 개발가치가 인정되나 대량 증식체계가 확립되지 않아 원예종으로 보급이 미흡한 새우난초 및 해오라비난초를 중심으로 종자의 기내발아와 유묘의 생장촉진에 미치는 배지의 영향에 대해 실험한 결과는 다음과 같다. 새우난초의 발아 및 유묘 생장은 파종전 초음파를 30분간 처리한 후 MS배지에 파종하여 얻은 유묘를 H$_3$P$_4$배지에 이식하여 육묘했을 때 가장 적합하였다. 해오라비난초는 종자를 H$_3$P$_2$배지에 파종하여 형성된 구근을 H$_3$P$_1$ 배지에 이식하여 맹아시킨 후 H$_1$P$_2$배지에 옮겨 배양했을 때 우량종구의 기내생산이 가능하였다.

Keywords

References

  1. Ph.D. thesis, Graduate school of Kyungpook Natl Univ Establishment of Propagation System and Selection of Genetic Variants through Asymbiotic Germination of Seed Shoot Tip of Temperate Zone Ochid, Cymbidium Species Choi SO
  2. Research Review of Kyungpook National Univ v.25 Studies on aseptic culture of seeds in Dendrobium monile.(Ⅰ) Effect of agar, sucrose, tryptone and peptone concentration on germination and growth Chun CK;Chung JD
  3. Korean J Plant Tissue Culture v.7 Seed culture of Neofinetia falcata in vitro.(Ⅱ) Effect of Hyponex medium added with various concentrations of peptone of tryptone on asymbiotic germination of seeds and growth of seedlings Chung JD
  4. Korean J Plant Tissue Culture v.8 Seed culture of Neofinetia falcata in vitro.(Ⅲ) Effect of auxin, kinetin, vitamin and apple juice on growth of seedlings after transplanting Chung JD
  5. Wild orchid Chung JD
  6. J Kor Soc Hort Sci v.24 Asymbiotic germination of Cymbidium ensifolium.(Ⅰ) Effect of basal media and growth regulators on germination of seeds and shoot emergence from rhizomes Chung JD;Chun CK
  7. J Kor Soc Hort Sci v.25 Asymbiotic germination of Aerides japonicum.(Ⅰ) Determination of optimal medium and cultural condition for germination of seeds and growth of seedlings Chung JD;Chun CK;Kim SS
  8. Korean J Plant Tissue Culture v.9 Studies on asymbiotic germination of seeds of Bletilla striata.(Ⅰ) Effect of basal media, light of dark treatment and auxius on germination of seeds and growth of seedling Chung JD;Suh JH
  9. Amer Jour Bot v.30 Germination and seedling development in five species of Cymbiditum Curtis JT
  10. Amer Orchid Soc Bull v.17 Studies on the nitrogen nutrition of orchid embriods.Ⅱ. Comparative utlization of nitrate and ammonium nitrogen Curtis JT;Spoerl E
  11. In culture method of Calanthe In vitro culture of Calanthe species Ento F
  12. Seed formation and asymbiotc germanation of Orchid Torkata H
  13. Jap J of Hort Sci(Abstract spring conference) Asymbiotic germination of orchid species(5) medium and its composition of inorganic salts Ichihashi S
  14. Fac Agri Kagawa Univ v.20 Studies on the media for orchid seed germination Mem. Kano K
  15. New plant tissue culture Use of plant tissue culture technique for propagation of horticultural crops Kano K;Takeuchi, K(et a.)
  16. Seed bearing and germination of Orchid species Medium for asymbtotic orchid seed germimation Kano K
  17. On asy,biotic germenation and growth of Laelia bregeri. Master thesis of graduate school. Choongbuk National University Lee HS
  18. Korean Orchid Seoul Lee J
  19. Scientia Horticulturae v.35 Ultrasonic treatment for enhancing seed germination of terrestrial orchid, in asymbiotic culture Miyoshi K;Mii M
  20. In kako,S. Bio technology of orchid Asymbiotic germination of cross seed of calanthe species Murakami G
  21. Gymnosperms and monocotyledons v.Ⅰ The classification of flowering plants Rendle AB
  22. Asymbiotic germination of Habenaria radiata Uetski B
  23. Bot Rev v.33 Use of tomato juice in the asymbiotic germination of orchid seeds Vacin EF;Went F