FLOWER RESEARCH JOURNAL (화훼연구)

The Korean Society for Floricultural Science (한국화훼학회)
권호 표지

Multiple Shoot Formation from Flower Stalk Tissue Culture of Phalaenopsis Hybrids

팔레높시스 화경절편조직 배양에 의한 다신초 형성

  • Choi, Jin Young (Department of Floral Design, Catholic University of Daegu) ;
  • Koh, Jae Chul (Department of Floral Design, Catholic University of Daegu)
  • 최진영 (대구가톨릭대학교 플라워디자인학과) ;
  • 고재철 (대구가톨릭대학교 플라워디자인학과)
  • Received : 2009.08.04
  • Accepted : 2009.09.24
  • Published : 2009.09.30

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Abstract

This experiment was conducted to extend utility of in-vitro culture material stalk and to mass produce uniform plants in Phalaenopsis. The optimal concentration of hyponex medium and plant hormone were examined. The most effective concentration of the hyponex in the flower stalk tissue culture of Phalaenopsis was $4mg{\cdot}L^{-1}$. The most effective concentration of TDZ and BA on the formation of multiple shoots in Phalaenopsis flower stalk culture was $0.3mg{\cdot}L^{-1}$ and $5mg{\cdot}L^{-1}$, respectively. TDZ was more effective for formation of the shoots and multiple shoots than BA at the basic medium with hyponex $4g{\cdot}L^{-1}$.

팔레높시스 화경의 모든 부위를 사용한 화경절편조직을 기내 배양하여 효율적이고 변이 발생률이 낮은 균일한 식물체를 일시에 대량생산하기 위하여 Hyponex와 생장조절물질 TDZ, BA의 적정 농도를 규명하고자 하였다. 두 공시 품종은 Hyponex, TDZ, BA 농도별로 floral stem, shoot, 다신초의 기관형성 발생에서 차이를 보였다. 팔레높시스의 화경절편조직 배양에 적절한 Hyponex의 농도는 $4g{\cdot}L^{-1}$ 가 가장 효과적이었으며, 다신초 형성에 효과적인 TDZ의 농도는 $0.3mg{\cdot}L^{-1}$이고, BA의 농도는 $5mg{\cdot}L^{-1}$ 이었다. 화경절편조직 배양 시 Hyponex $4g{\cdot}L^{-1}$을 기본배지로 하여 TDZ와 BA의 효과를 비교한 결과 TDZ가 더 효과적이었다.

Keywords

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