FLOWER RESEARCH JOURNAL (화훼연구)

The Korean Society for Floricultural Science (한국화훼학회)
권호 표지

Induction and Propagation of Protocom-Like Bodies from Shoot Tip Culture in the Pansy Orchid (Miltonia spp.)

경정배양에 의한 밀토니아의 PLB 유기와 기내 증식

  • Kim, Mi-Seon (National Institute of Horticulture & Herbal Science) ;
  • Hwang, Sun-Ja (National Institute of Horticulture & Herbal Science) ;
  • Kim, Jae-Yeong (National Institute of Horticulture & Herbal Science) ;
  • Choi, I-Jin (Dept. of Plant Biotechnology, Dongguk University)
  • 김미선 (국립원예특작과학원 화훼과) ;
  • 황선자 (국립원예특작과학원 화훼과) ;
  • 김재영 (국립원예특작과학원 화훼과) ;
  • 최이진 (동국대학교 식물생명공학과)
  • Received : 2008.07.02
  • Accepted : 2009.03.09
  • Published : 2009.03.31

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Abstract

This study was conducted to find out the commercial in vitro propagation methods through protocom-like bodies (PLB) induced from shoot tip culture of Miltonia spp. Among several culture media for induction PLB from shoot tip in Miltonia spp., MS basal medium was better than Hyponex, Vacin & Went basal medium and other media supplemented with natural additives. PLB's proliferation and differentiation in Hyponex medium including $20g{\cdot}L^{-1}$ banana + $20g{\cdot}L^{-1}$ sucrose(pH 5.2) was better than in MS medium. It was tendency that solid media showed higher PLB fresh weight than liquid medium or other cotton bridge culture. The dark culture for 1~2 weeks and adding $10{\sim}20g{\cdot}L^{-1}$ sucrose on hyponex basal medium was the most effective to increase the PLBs growth and shoot number.

밀토니아의 기내 대량생산을 위해 PLB의 유도와 증식을 위한 적정 배지 및 배양방법을 찾고자 본 실험을 수행하였다. 적정 배지는 MS 기본배지가 Hyponex 또는 VW배지보다 밀토니아의 PLB 유도가 우수하였다. PLB 증식 및 신초분화는 Hyponex 고체배지(Hyponex +바나나 20 g/L + sucrose $20g{\cdot}L^{-1}$, pH 5.2)가 MS 배지에 비해 더욱 효과적이었다. PLB로부터 신초분화에는 sucrose를 $10{\sim}20g{\cdot}L^{-1}$ 첨가하는 것이 효과적이었으나 30 g 이상의 농도에서는 유도된 신초수가 크게 감소하였다.

Keywords

References

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