• Environmental Analysis Health and Toxicology
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• 제13권3_4호
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• pp.105-115
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• 1998

Reactive oxygen species (ROS) are highly reactive molecules due to their unpaired electron. They have been suspected as one of the major tissue damage inducers in biological metabolic systems. Antioxidant enzymes, such as catalase and superoxide dismutase, could not repair all the oxidative damages resulting from those excessive toxic ROS. It is, therefore, urgent to develop effective antioxidants to relieve from the oxidatire damages. In this study antioxidative effects were investigated by using two flavonoids such as quercetin and naringenin and a flavonoid-rich extract, Ginkgo biloba extract in combination with paraquat that is known as a strong generator of oxygen radicals. The results are summeringed as follows: 1. To assess radical scavenging ability reduction concentrations (IC$_{50}$) of 1,1-diphenyl-2-picrylhydrazine (DPPH) within 15 minutes were measured. The values of the IC$_{50}$ of quercetin and Ginkgo biloba extract were 15.4 $\mu$M and 13.2$\mu$g/ml, respectively. Their radical removing activities showed concentration-dependent manners. 2. In the hydrogen peroxide assay by using PMS-NADH system, quercetin, naringenin and Ginkgo biloba extract led to removing hydrogen peroxide in concentrationdependent manner whose removing abilities at 100$\mu$M or 100 $\mu$g/ml were 75.6, 25.8 and 26.0%, respectively. 3. In the hydrogen peroxide-induced rat blood hemolysis assay all three compounds led to similar effects whose hemolysis inhibition ratios at 100$\mu$M or 100$\mu$g/ml were 68.0, 5.14 and 55.8%, respectively. 4. In the xanthinee oxidase assay by measuring degree of NADH oxidation in the presence of hypoxanthine and xanthinee oxidase, both quercetin and Ginkgo biloba extract showed excellent activities showing 42.8 and 24.2% inhibiting xanthine oxidase activity at 100$\mu$M or 100$\mu$g/ml concentrations, respectively.

• Journal of Ginseng Research
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• 제23권3호
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• pp.176-181
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• 1999

홍삼에서 분리한 수용성 갈변물질의 항산화 활성을 DPPH 수소공여능 및 hydrogen peroxide 시스템에서 조사한 결과를 다음과 같다. 1. DPPH 수소공여능 수용성 갈변물질 S-1, S-2는 반응시간이 경과함에 따라 완만하게 감소하였고, L은 S-1, S-2보다 시간이 경과 함에 따라 감소폭이 증가하였다. L의 경우 BHT, BHA $5{\times}10^{-4}M,\;{\alpha}-tocopherol\;1{\times}10^{-4}M$ 및 ascorbic acid $5.7{\times}10^{-3}M$ 보다 감소폭이 증가하였으나, S-1, S-2는 합성 항산화제와 감소폭이 비슷하였다. 2. Synergist 효과 L, S-1 및 S-2 와 상기의 합성 항산화제 농도와 시너지스트 효과를 조사한 결과, 모든 시험구에서 반응 시간이 경과 함에 따라 감소폭이 증가하여 시너지스트 효과가 나타났다. 3. Hydrogen Peroxide 소거능 :수용성 갈변물질의 hydrgen peroxide 소거능을 조사한 결과, L은 $50\~80\%$, S-1은 $70\~100\%,$ S-1은 모든 첨가구에서 반응 초기부터 $100\%$로 완전히 소거되었다.

• 대한약침학회지
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• 제13권2호
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• pp.5-12
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• 2010

본 연구의 목적은 자하거약침액의 항산화기능에 관련한 생리활성을 보기 위하여 대한약침학회에서 제공받은 자하거 약침액을 시료로 사용하였다. 총 폴리페놀 함량은 자하거 약침액 원액에서 24.641 mg/L로 이는 산수유 등과 같은 약용식물과 비슷한 수준의 함량이다. 자하거약침액원액에서 DPPH radical 소거능과 Superoxide dismutase(SOD) 유사활성 측정은 각각 49.4%, 50.87%였다. 또한 ABTS radical 소거능과 Nitrite radical 소거능(pH1.5)에서는 각각 50.01%, 52.8%로 ABTS radical과 Nitrite radical 소거능이 우수하였다. 본 실험결과로볼 때, 자하거 약침액은 항산화능력은 뛰어난 것으로 사료된다. 하지만 현재 자하거 약침액과 관련된 실험이 부족한 실정이기 때문에 앞으로 이에 관련된 더 많은 연구가 필요하다고 생각된다.

• Toxicological Research
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• 제25권4호
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• pp.243-251
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• 2009

Present study was conducted to evaluate the anti oxidative activity of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidative damage. The antioxidative activity of methanolic (MeOH) extract of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidation, including liposome oxidation, deoxyribose oxidation, protein oxidation, chelating activity against metal ions, scavenging activity against hydrogen peroxide, scavenging activity against hydroxyl radical and 2'-deoxyguanosine (2'-dG) oxidation were investigated. The MeOH extract of the Agrimonia pilosa-Ledeb leaves exhibited high anti oxidative activity in the liposome model system. Deoxyribose peroxidation was inhibited by the MeOH extract of the Agrimonia pilosa-Ledeb leaves and MeOH extract of the Agrimonia pilosa-Ledeb leaves provided remarkable protection against damage to deoxyribose. Protective effect of MeOH extracts of the Agrimonia pilosa-Ledeb leaves on protein damage was observed at $600{\mu}g$ level (82.05%). The MeOH extracts of the Agrimonia pilosa-Ledeb leaves at $300{\mu}g$ revealed metal binding ability (32.64%) for hydrogen peroxide. Furthermore, the oxidation of 2'-deoxyguanosine (2'-dG) to 8-hydroxy-2-deoxyguanosine (8-OH-2'dG) was inhibited by MeOH extracts of the Agrimonia pilosa-Ledeb leaves and scavenging activity for hydroxyl radical exhibited a remarkable effect. From the results in the present study on biological model systems, we concluded that MeOH extract of the Agrimonia pilosa-Ledeb leaves was effective in the protection of non-lipids against various oxidative model systems.

• 미생물학회지
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• 제46권4호
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• pp.375-382
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• 2010

갓김치로부터 분리된 유산균 5종(Lactobacillus acidophilus GK20, Lactobacillus brevis GK55, Lactobacillus paracasei GK74, Lactobacillus plantarum GK81, 및 Leuconostoc mesenteroides GK104)의 활성산소종에 대한 저항성 및 항산화 활성을 조사하였다. Hydrogen peroxide (0.5 mM)와 반응 5시간후 L. acidophilus GK20, L. brevis GK55, L. paracasei GK74 및 L. plantarum GK81들은 50% 이상의 생존율을 보였으며, superoxide anions에 의한 저해율은 L. acidophilus GK20와 L. paracasei GK74가 가장 낮았고, hydroxyl radical과의 반응에서는 L. paracasei GK74와 L. plantarum GK81이 가장 안정하였다. 한편, L. plantarum GK81는 실험 균주 중 가장 높은 DPPH 소거능($70.8{\pm}10.1%$)을 나타내었는데, 세포(IC)보다는 세포추출물(ICFE)의 활성이 더 높았다. 또한 L. plantarum GK81의 ICFE는 다른 균주들에 비해 높은 superoxide radical 소거능을 보였고, 이 균주의 IC와 ICFE에 의한 환원력은 BHA와 vitamin C보다 유의적으로 더 높게 나타났으며, L.paracasei GK74 IC의 환원력도 vitamin C와 비슷한 수준으로 나타났다. L. brevis GK55, L. paracasei GK74 및 L.mesenteroides GK104 IC의 $Fe^{2+}$-chelating 활성은 30% 이상이었고, L. plantarum GK81의 ICFE는 45% 이상의 활성을 나타내었다. 따라서 실험 균주 중 L. plantarum GK81은 다른 균들에 비해 ROS에 대해 비교적 안정하고, 항산화 활성도 가장 높은 것으로 확인되었다.

• 한국식생활문화학회지
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• 제38권2호
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• pp.112-118
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• 2023

Seaweed-derived foods have long been popular in Korea because of their high content of nutrients that are beneficial to the human body. Recently, Korean seaweeds have been used as raw materials to produce new natural products with health benefits. Herein, we compared the antioxidant activity of 16 Korean seaweed extracts to explore their potential utility as health foods. The total phenolic content (TPC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of seaweed extracts were determined. We also investigated their ability to protect human diploid fibroblast (HDF) cells against hydrogen peroxide. The results showed that seaweed extracts at a concentration of 100 ㎍/mL did not cause any cell toxicity. Sargassum thunbergii (Jichung-i) had the highest TPC and radical scavenging effects, followed by Porphyra tenera (Gim), Silvetia siliquosa (Tteumbugi), and Sargassum fusiforme (Tot). Hydrogen peroxide increased the production of intracellular reactive oxygen species, while P. tenera (Gim), Saccharina japonica (Dasima), and S. thunbergii (Jichung-i) extracts significantly decreased it. The effect was highest in the S. thunbergii (Jichung-i)-treated HDF cells. These findings indicate that S. thunbergii (Jichung-i) shows promise as a potential antioxidant raw material.

• Asian-Australasian Journal of Animal Sciences
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• 제25권8호
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• pp.1153-1158
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• 2012

The objective of this study was to evaluate the antioxidant capacity of Lactobacillus plantarum ZLP001 and its effects on growth performance and antioxidant status in weaning piglets. The survival in hydrogen peroxide and free radical-scavenging activity of Lactobacillus plantarum ZLP001 were analysed in vitro. The Lactobacillus plantarum ZLP001 showed high viability in 1.0 mmol/L hydrogen peroxide and high scavenging ability against hydroxyl, superoxide anion, and DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals which was dose dependent. Ninety-six weaning piglets were selected ($7.45{\pm}0.79kg$) and divided into three groups comprising of negative control without any supplementation, treatment group with supplemented $6.8{\times}10^7$ Lactobacillus plantarum ZLP001 CFU/g of diet, and positive control with antibiotic treatment (chlorotetracycline, 80 mg/kg diet). The results showed that Lactobacillus plantarum ZLP001 supplementation enhanced feed conversion rates in piglets compared with control (p<0.05). Supplementation of Lactobacillus plantarum ZLP001 increased the concentration of superoxide dismutase (p<0.05), glutathione peroxidase (p<0.01) and catalase in serum (p<0.10), while decreased the concentration of malondialdehyde (p<0.05). The present study implies that the strain Lactobacillus plantarum ZLP001 had high antioxidant ability and its supplementation improved the growth performance and antioxidant status of weaning piglets, so it can be considered useful to alleviate oxidative stress and increase productive performance of pigs.

• Food Science and Biotechnology
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• 제15권5호
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• pp.728-734
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• 2006

The tannins represent a highly heterogeneous group of water-soluble plant polyphenols that may play an important role in antimutagenic and antioxidant properties. We investigated the antioxidant function of tannic acid in comparison to other phenolic compounds including catechin, chlorogenic acid, cinnamic acid, ellagic acid, and gallic acid for their ability to scavenge several stable radicals and reactive oxygen species (ROS) such as ${\bullet}DPPH^+$, ${\bullet}ABTS^+$, hydrogen peroxide, hydroxyl radical, and superoxide radical. The ability of tannic acid to decrease paraquat-induced lipid oxidation in mouse liver and lung through its antioxidant properties was also assessed. The results showed that almost all the tested compounds have stable radical scavenging activity except cinnamic acid. Tannic acid, gallic acid, and ellagic acid demonstrated remarkable ROS scavenging properties toward $H_2O_2$, ${\bullet}OH^-$, ${\bullet}O_2^-$ and especially only tannic acid could inhibit paraquat-induced lipid peroxidation effectively in mouse liver and lung. Based on these results, it appears that increased number of galloyl and ortho-hydroxyl groups enhances the antioxidant activity of phenolic compounds and tannic acid is evaluated as the most effective antioxidant among all the tested compounds. These results suggest that the tannins, especially tannic acid, can be used as therapeutic agent for various diseases caused by ROS.

• 생명과학회지
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• 제24권11호
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• pp.1193-1199
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• 2014

국내의 천연자원 및 부산물로부터 기능성을 갖는 다양한 물질을 탐색하여 자원의 효율적인 측면, 새로운 기능성을 함유한 화장품소재 개발 및 피부 노화 메커니즘을 연구함으로써 밤과수 과정에서 폐기되는 밤송이의 활용방안을 찾기 위한 연구의 기초 자료로서 그 화학성분을 분석하였으며, 또한 새로운 기능성 소재를 탐색하기 위한 일환으로 밤송이 발효 추출물을 이용하여 항산화 효과에 대한 연구를 하였다. DPPH 라디칼소거능, Hydrogen peroxide저해활성, Nitric oxide radical 소거능 측정결과 균주 5% 접종 후 6일간 배양액 추출에서 가장 높은 항산화활성을 나타내었다. 또한 ABTS 라디칼소거능, 환원력 측정, Superoxide anion radical 저해활성 실험을 통해서 대조군으로 사용된 BHA와 Ascrobic acid, EGCG에 비교하여 발효밤송이 추출물은 유사한 항산화 활성을 나타나는 결괄를 얻었다. 이와 같은 결과로 미루어 보아 발효 밤송이 추출물은 효능이 좋은 천연 항산화제로 기능성 화장품의 소재로 개발될 가능성이 매우 높다고 사료된다.

• 생명과학회지
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• 제15권5호
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• pp.796-801
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• 2005

국내산 산죽잎을 methanol로 추출하여 천연 항산화제로서의 이용성을 조사하기 위하여 여러 방법으로 항산화 활성을 측정하고 안정성에 대해 조사하였다. 산죽잎 메탄올 추출물은 0.1 mg/l에서 0.8mg/ml의 범위에서 농도 의존적으로 항산화활성이 증가하였다. 산죽잎 메탄올추출물은 DPPH radical 소거능에 대한 $IC_{50}$ 값은 $583{\mu}g/ml$, SOD 유사활성능에 대한 $IC_{50}$값은 $800{\mu}g/ml$, hydrogen peroxide 소거능에 대한 $IC_{50}$값은 $38{\mug}/ml$이 었으며 대조구로 사용한 BHT의 각각 의 $IC_{50}$값은 $271{\mu}g/ml,{\;}680{\mu}g/ml,{\;}30{\mu}g/ml$이었다. 산죽잎 메탄올추출물의 자동산화 억제능은 상기 농도 범위에서 $55\∼60\%$의 억제효과를 나타내었다. 산죽잎 메탄올추출물의 pH 안정성은 pH 6에서 안정하였으나 산성이나 알카리성으로 갈수록 pH 안정성은 떨어졌다. 산죽잎 메탄올추출물의 열안정성은 $0^{\circ}C$에서 $120^{\circ}C$의 온도범위에서 $80\%$ 이상의 항산화능을 유지하였다.