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http://dx.doi.org/10.5487/TR.2009.25.4.243

Evaluation of Antioxidative Activity of Agrimonia pilosa-Ledeb Leaves on Non-lipid Oxidative Damage  

Hah, Dae-Sik (Gyeongnam Livestock Promotion Institute Middle-branch)
Kim, Chung-Hui (Department of Animal Science and Biotechnology, Jinju National University)
Kim, Eui-Kyung (College of Veterinary Medicine, Gyeongsang National University (Institute of Animal Science))
Kim, Jong-Shu (College of Veterinary Medicine, Gyeongsang National University (Institute of Animal Science))
Publication Information
Toxicological Research / v.25, no.4, 2009 , pp. 243-251 More about this Journal
Abstract
Present study was conducted to evaluate the anti oxidative activity of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidative damage. The antioxidative activity of methanolic (MeOH) extract of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidation, including liposome oxidation, deoxyribose oxidation, protein oxidation, chelating activity against metal ions, scavenging activity against hydrogen peroxide, scavenging activity against hydroxyl radical and 2'-deoxyguanosine (2'-dG) oxidation were investigated. The MeOH extract of the Agrimonia pilosa-Ledeb leaves exhibited high anti oxidative activity in the liposome model system. Deoxyribose peroxidation was inhibited by the MeOH extract of the Agrimonia pilosa-Ledeb leaves and MeOH extract of the Agrimonia pilosa-Ledeb leaves provided remarkable protection against damage to deoxyribose. Protective effect of MeOH extracts of the Agrimonia pilosa-Ledeb leaves on protein damage was observed at $600{\mu}g$ level (82.05%). The MeOH extracts of the Agrimonia pilosa-Ledeb leaves at $300{\mu}g$ revealed metal binding ability (32.64%) for hydrogen peroxide. Furthermore, the oxidation of 2'-deoxyguanosine (2'-dG) to 8-hydroxy-2-deoxyguanosine (8-OH-2'dG) was inhibited by MeOH extracts of the Agrimonia pilosa-Ledeb leaves and scavenging activity for hydroxyl radical exhibited a remarkable effect. From the results in the present study on biological model systems, we concluded that MeOH extract of the Agrimonia pilosa-Ledeb leaves was effective in the protection of non-lipids against various oxidative model systems.
Keywords
Antioxidative activity; Agrimonia pilosa leaves; 2'-Deoxyguanosine (2'-dG); Non-lipid oxidative model systems;
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1 Antosiewicz, J., Popinigis, J., Wozniak, M., Damiani, E., Carloni, P. and Greci, L. (1995). Effects of indolinic and quinolinic aminoxylson protein and lipid peroxidation of rat liver microsoned. Free Rad. Bio. Med., 18, 913-917   DOI   ScienceOn
2 Aruoma, O.I. (1991). Pro-oxidant properities: An important consideration for food additive and/or nutrient components.: In free radical and food additives (O.I. Aruoma and B. Hailliwell, Eds.). Taylor & Francis, London, pp 173-194
3 Benzie, I.F.F. (2000). Evolution of antioxidant defence mechanism. Eur. J. Nutr., 39, 53-21   DOI   PUBMED   ScienceOn
4 Carter, P. (1971). Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine). Analy. Biochem., 40, 450-458   DOI   PUBMED   ScienceOn
5 Cha, B.C., Lee, S.K., Lee, H.W, Lee, U., Choi, M.Y., Rhim, T.J. and Park, H.J. (1997). Antioxidative effects of medicinal plants in Korea. Kor. J. Pharmacogn., 28, 15-20
6 Frankel, E.N., Huang, S.W. and Aeschbach, R. (1997). Antioxidant ativity of green teas in different lipid systems. J. Ame. Oil Chern. Soc., 74, 1309-1315   DOI   ScienceOn
7 Isao, K., Naosuke, B., Yumiko, O. and Nobusuke, K. (1988). Triterpenoids from Agrimonia pilosa. Phytochemistry, 27, 297-299   DOI   ScienceOn
8 Karioti, D., Hadjipavlou, L., Mensah, M.L., Fleischer, TC. and Skaltsa, H. (2004). Composition and antioxidant activity of the essential oils of Xylopia aethiopica (Dun) A Rich. (Annonaceae) leaves, stem bark, root bark, and fresh and dried fruits, growing in Ghana. J. Agri. Food Chemi., 52, 8094-8098   DOI   ScienceOn
9 Kimie, I., Tachio, A, Masaki, S., Yoshisa, K., Ryokei, K. and Toshiro, M. (1988). Antioxidative effect of protoporphyrin on lipid rat liver subcellular fractions. Chem. Pharm. Bull., 36, 1104-1109   DOI   PUBMED   ScienceOn
10 Nobuyki, S., Shunji, U., Yoshinori, F. and Masayasu, S. (1996). Protective effect of vitamin E on chromium (VI)induced cytotoxicity and lipid peroxidation in primary cultures of rat hepatocytes. Arch. Toxicol., 71, 20-24   DOI   ScienceOn
11 Smith, C., Halliwell, B. and Aruoma, O.I. (1992). Protecting byalbumin against the pro-oxidant action of phnolic dietary components. Food Chem. Toxico., 30, 483-489   DOI   ScienceOn
12 Wen, H.C., Beth, A.V. and Xin, G.L. (1999). High level dietary vitamin E do not replace cellular glutathione peroxidase in protecting mice from acute oxidative stress. Biochem. Mole. Action Nutr., 98, 1951-1957
13 Yun, Z.F., Sheng, Y. and Guoyao, W. (2002). Free radicals, antioxidants, and Nutrition. Nutrition, 18, 872-879   DOI   ScienceOn
14 Lennon, S.V., Marin, S.J. and Cotter, T.G. (1991). Dosedependent induction of apoptosis in human tumor cell lines by widely diverging stimuli. Cell Prolife., 24, 203-14   DOI   ScienceOn
15 Fridovich, I. (1974). Superoxide and evolution. Horizons Biochem. Biophys., 1, 1-37   PUBMED
16 Hatano, T. (1995). Constituents of natural medicines with scavenging effects on active oxygen species-Tannins and related polyphenols. Natu. Medi., 49, 357-363
17 Masaki, H., Sakaki, S., Atsumi, T. and Sakurai, H. (1995). Active-oxygen scavenging activity of plant extracts. BioI. Pharm. Bull., 18, 162-166   DOI   ScienceOn
18 Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M. and Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease, Inter. J. Biochem. Cell Bio., 39, 44-84   DOI   ScienceOn
19 MeCord, J.M. (2000). The evolution of free radicals and oxidative stress. Am. J. Med., 108, 652-662   DOI   PUBMED   ScienceOn
20 Hah, D.S., Kim, C.H., Kim, E.G and Kim, J.S. (2005). Antioxidative effects of traditional medicinal plants on lipid peroxidation. Korea J. Vet. Res., 45, 341-350
21 Ayres, G.H. (1949). Evaluation of accuracy in photometric analysis. Anal. Chem., 21, 652-657   DOI
22 Aruoma, O.I., Spencer, J.P. and Mahmood, E. (1999). Protection against oxidative damage and cell death by the natural antioxidant Ergothineine. Food and Chem. Toxieol., 37, 1043-1053   DOI   ScienceOn
23 Fang, Y.Z., Yang, S. and Wu, G. (2002). Free radical, antioxidants and nutrition. Nutr., 18, 872-879   DOI   ScienceOn
24 Halliwell, B. (1991). Drug antioxidant effects. Drugs, 42, 569-605   DOI   PUBMED   ScienceOn
25 Imlay, J.A. and Linn, S. (1988). DNA damage and oxygen radical toxicity. Science, 240, 1302-1309   DOI   PUBMED
26 Sloane, H.B. and William, S.G. (1977). Spectrophotometric accuracy, linearity and adherence to Beer's law. Appl. Spectro., 31, 25-30   DOI   ScienceOn
27 Ames, B.N. and Gold, L.S. (1991). Endogenous mutagens and cause of aging and cancer. Mut. Res., 250, 3-16   DOI   ScienceOn
28 Imaida, K., Fukushima, S., Hivai, T., Ohtani, M., Nakanish, K. and Ito, N. (1983). Promoting activitied of butylated hydroxyanisol and butylated hydroxytpluene on 2-stage urinary bladder carcinogenesis and inhibition of r-glutamyl transpeptase-positive foci development in the liver of rats. Carcino., 4, 895-899   DOI   ScienceOn
29 Ozcelik, B., Lee, J.H. and Min, D.B. (2003). Effects of light, oxygen, and pH on the absorbance of 2,2-diphenyl-1-picrylhydrazyl. J. Food Sci., 68, 487-490   DOI   ScienceOn
30 Tamura, H. and Shibamoto, T. (1991). Antioxidative activity measurement in lipi peroxidation system with malonaldehyde and 4-hydroxy nonenal. J. Am. Oil Chem. Soc., 68, 941-943   DOI
31 Branen, A.L. (1975). Toxicology and biochemistry of butylated hydroxy anisole and butylated hydroxy toluene. J.A.O.C.S., 52. 59-63   DOI
32 Lenz, AG, Costable, U., Shaltei, S. and Cevine, RL. (1989). Determinationof carboyl groups inoxidatively modified proteins by reduction withtritiated sodium borohydride. Analy. Biochem., 177, 419-425   DOI   ScienceOn
33 Park, J.H., Kang, K.C., Baek, S.B., Lee, YH. and Rhee, K.S. (1991). Separation of antioxidants compounds from edible marin algae. Korean J. Food Sci. Technol., 23, 256-261
34 Zhao, M.J. and Jung, L. (1995). Kinetis of the competive degradation of deoxyribose and other molecules by hydroxyl radicals produced by the Fenton reaction in the presence of ascorbic acid. Free Rad. Res., 23, 229-243   DOI   ScienceOn
35 Duh, P.D., Du, P.C. and Yen, G.C. (1999). Action of methanolic extract of Mung bean hulls as inhibitors of lipid peroxidation and non-lipid oxidative damage. Food Chem. Toxicol., 37, 1055-1061   DOI   ScienceOn
36 Freidovich, I. (1999). Fundamental aspects of reactive oxygen species, or what's the matter with oxygen? Ann. NY Acad. Sci., 893, 13-15   DOI
37 Halliwell, B., Gutteridge, J.M.C. and Aruoma, O.I. (1987). The deoxyribose method: a simple 'teste-tube' assay for determination of rate constants for reaction of hydroxyl radicals. Anal. Biochem., 165, 215-219   DOI   ScienceOn
38 Chen, G. and Stevens, J.L. (1991). Inhibition of iodoacetamide and t-butyl hydroperoxide toxicity in LLC-PKI cells by antioxidants: a role for lipid peroxidation in alkylation induced cytotoxicity. Arch. Biochern. Biophy., 284, 422-430   DOI   ScienceOn
39 Su, G., Su, S. and Zhu, T. (1984). Studies on bacteriostatic components from Agrimonia pilosa Ledeb. Shenyang Yaoxueyuan Xuebao., 1, 44-50
40 Strain, J.J. and Benzie, I.F.F. (1999). Free radicals in biology and medicine. Clarendon Press, Oxford. Antioxidants: diet and antioxidant defence. In: The Encyclopedia of human nutrition (M. Sadler, J.J. Strain and B. Cabellero, Eds.). Academic Press, London, pp. 95-106
41 Ruch, K.J., Cheng, S.J. and Klauing, J.E. (1989). Prevention of cytotoxity and inhibition of intracellular communication by antioxidant catechin isolated from Chinese green tea. Carcinogenesis., 10, 1003-1008   DOI   ScienceOn
42 Droge, W. (2002). Free radicals in the physiological control of cell function. Physiological Reviews, 82, 47-95   PUBMED
43 Stadtman, H. and Shibamoto, T. (1991). Antioxidative activity measurement in lipid peroxidation system with malonaldehyde and 4-hydroxy nonenal. J. Am. Oil Chem. Soc., 68, 941-943   DOI
44 Sala, A, Recio, M.C., Schinella, GR, Manez, S., Giner, RM., Cerda, N.M. and Rios, J.L. (2003). Assessment of the anti-inflammatory activity and free radical scavenger activity of tiliroside. Eur. J. Pharm., 461, 53-61   DOI   ScienceOn
45 Schreck, R., Rieber, P. and Baeuerle, P.A. (1991). Reactive oxygen intermediate as apparently widely used messengers in the activation of the NFkB transcription factor and HIV-1. EMBO J., 10, 2247-2258   PUBMED
46 Eriksson, C.E. (1987). Oxidation of lipids in food systems: In Autooxidation of unsaturated lipids (H.W.S. Chan, Ed.). Academic Press, London, pp. 207-231
47 Shi, X., Dalal, N.S. and Jain, A.C. (1991). Antioxidant behaviour of caffeine: efficient scavenging of hydroxyl radicals. Food Chem. Toxicol., 29, 1-6   DOI   ScienceOn
48 Salles, B., Sattler, U., Bozzato, C. and Calsou. E. (1999). Repair of oxidative DNA damage in vitro: A tool for screening antioxidative compounds. Food Chem. Toxico., 37, 1009-1014   DOI   ScienceOn
49 Cha, B.C., Park, H.J., Lee, E., Choi, MY and Rhim, T.J. (1996). Comparison of antioxidant activity and composition in Glycine max. and Glycine soja Siebold et Zucco. Kor. J. Pharmacogn., 27, 190-195
50 Kasai, H. and Nibishimura, S. (19894). Hydroxylation of deoxyguanisine at the C-8 position by ascorbic acid and other reducing agents. Nucleic Acids., 12, 2137-2145   DOI   ScienceOn
51 Selvendiran, K.J., Singh, P.v., Kirshnan, K.B. and Sakthisekaran, D. (2003). Cytoprotective effect of piperine against beno[a]pyrene induced lung cancer with reference to lipid peroxidation and antioxidant system in Swiss albino mice. Fitoterapia, 74, 109-115   DOI   ScienceOn
52 Sohal, R.S. and Weindruch, .R (1996). Oxidative stress, caloric restriction, and agibg. Sci., 128, 372-379
53 Anne, C.T. and Andrew, R.C. (2000). Recovery of human lymphocytes from oxidative DNA damage; the apparent enhancement of DNA repair by cartenoids is probably simply an antioxidant effect. Eur. J. Nutr., 39, 80-85   DOI   ScienceOn
54 Ginn, P.M.E. and Hisler, R.L. (1998). Redxo signals and NFkB activation in T cell. Free Rad. Bio. Med., 25, 346-361   DOI   ScienceOn
55 Kano, M., Takayanagi, T., Harada, K., Makino, K. and Ishikawa, F. (2005). Antioxidative activity of anthocyan ins from purple sweet potato Ipomoera batatas cultivar Ayamurasaki. Biosci. Biotechno. and Biochemi., 69, 979-988   DOI   ScienceOn
56 Pei, Y.H., Li, X. and Zhu, T.R. (1989). Studies on the chemical constitutes from the root-sprouts of Agrimonia pilosa Ledeb. Yao. Hsueh Hsueh Pao., 25, 267-270
57 Floyd, R.A. (1990). Role of oxygen free radicals in carcinogenesis and brain ischemia. FASEB, 4, 2587-2597
58 Hah, D.S., Kim, C.H., Kim, E.G., Kang, J.B. and Kim, J.S. (2007). Antioxidative effects of Houttuynia cordata root on non-lipid oxidative damage. Korea J. Vet. Res., 47, 25-32
59 Lander, H.M. (1997). An essential role for free radicals and derived species in signal transduction. FASEB J., 11, 118-126   PUBMED
60 Neuzil, J., Gebicki, J.M. and Stocker. R. (1993). Radicalinduced chain oxidation of proteins and its inhibition by chain breaking antioxidants. Biochern. J., 293, 601-606
61 Eklund, P.C., Langvik, O.K, Wama, J.P, Salmi, T.O., Willfor, S.M. and Sjoholm, R.E. (2005). Chemical studies on antioxidant mechanisms and free radical scavenging properties of lignans. Orga. Bimolec. Chem., 21, 3336-3347
62 Pratt, D.E. and Watts, B.W. (1964). The antioxidative activity of vegetable extracts. I. Flavone aglycones. J. Food Sci., 29, 17-24
63 Gutteridge, J.M.C. and Stocks, J. (1981). Caeruloplasmin : physiological and pathological perspectives. Crit. Rev. Clin. Labo. Sci., 14, 257-329   DOI   ScienceOn
64 Inatani, R, Nakatani, N. and Fuwa, H. (1996). Antioxidative effect the constituent of rosemary and their derivatives. Agric. BioI. Chem., 47, 521-525
65 Fukuzawa, K. and Takaishi, Y. (1990). Antioxidants. J. Act. Oxyg. Free Rad., 1, 55-70