• 한국미생물생명공학회:학술대회논문집
• /
• 한국미생물생명공학회 2002년도 학술발표대회
• /
• pp.18-25
• /
• 2002

The pikromycin biosynthetic system in Streptomyces venezuleae is unique for its ability to produce two groups of antibiotics that include the 12-membered ring macrolides methymycin and neomethymycin, and the 14-membered ring macrolides narbomycin and pikromycin. The metabolic pathway also contains two post polyketide-modification enzymes, a glycosyltransferase and P450 hydroxylase that have unusually broad substrate specificities. In order to explore further the substrate flexibility of these enzymes a series of hybrid polyketide synthases were constructed and their metabolic products characterized. The plasmid-based replacement of the multifunctional protein subunits of the pikromycin PKS in S. venezuelae by the corresponding subunits from heterologous modular PKSs resulted in recombinant strains that produce both 12- and 14-membered ring macrolactones with predicted structural alterations. In all cases, novel macrolactones were produced and further modified by the DesVII glycosyltransferase and PikC hydroxylase leading to biologically active macrolide structures. These results demonstrate that hybrid PKSs in S. venezuelae can produce a multiplicity of new macrolactones that are modified further by the highly flexible DesVII glycosyltransferase and PikC hydroxylase tailoring enzymes. This work demonstrates the unique capacity of the S. venezuelae pikromycin pathway to expand the toolbox of combinatorial biosynthesis and to accelerate the creation of novel biologically active natural products. The polyketide backbone of rifamycin B is assembled through successive condensation and ${\beta}$-carbonyl processing of the extender units by the modular rifamycin PKS. The eighth module, in the RifD protein, contains nonfunctional DH domain and functional KR domain, which specify the reduction of the ${\beta}$-carbonyl group resulting in the C-21 bydroxyl of rifamycin B. A four amino acid substitution and one amino acid deletion were introduced in the putative NADPH binding motif in the proposed KR domain encoded by rifD. This strategy of mutation was based on the amino acid sequences of the corresponding motif of the KR domain of module 3 in the RifA protein, which is believed dysfunctional, so as to introduce a minimum alteration and retain the reading frame intact, yet ensure loss of function. The resulting strain produces linear polyketides, from tetraketide to octaketide, which are also produced by a rifD disrupted mutant as a consequence of premature termination of polyketide assembly. Much of the structural diversity within the polyketide superfamily of natural products is due to the ability of PKSs to vary the reduction level of every other alternate carbon atom in the backbone. Thus, the ability to introduce heterologous reductive segments such as ketoreductase (KR), dehydratase (DH), and enoylreductase (ER) into modules that naturally lack these activities would increase the power of the combinatorial biosynthetic toolbox. The dehydratase domain of module 7 of the rifamycin PKS, which is predicted to be nonfunctional in view of the sequence of the apparent active site, was replaced with its functional homolog from module 7 of rapamycin-producing polyketide synthase. The resulting mutant strain behaved like a rifC disrupted mutant, i.e., it accumulated the heptaketide intermediate and its precursors. This result points out a major difficulty we have encountered with all the Amycolatopsis mediterranei strain containing hybrid polyketide synthases: all the engineered strains prepared so far accumulate a plethora of products derived from the polyketide chain assembly intermediates as major products instead of just analogs of rifamycin B or its ansamycin precursors.

• 전자공학회논문지D
• /
• 제34D권5호
• /
• pp.52-60
• /
• 1997

Photonic switching systems with throughput above Tbit/s are reuried to transport vast amounts of information for the coming B-ISDN. In this paper, we proposed a new time-division hybrid WDM photonic swithc architecture, the proposed basic switch module has simple configuratin consisted of frequency routers for wavelength division and cell coders and star couplers for time division. Through the comparison with other sysems in field of switching capacity, hardware complexity and cost effect of implemetnation, we proved that the proposed system is suitable for large-capacity photonic switching system.

• 한국세라믹학회지
• /
• 제54권4호
• /
• pp.272-277
• /
• 2017

Organic-inorganic hybrid thermoelectric materials have obtained increasing attention because it opens the possibility of enhancing thermoelectric performance by utilizing the low thermal conductivity of organic thermoelectric materials and the high Seebeck coefficient of inorganic thermoelectric materials. Moreover, the organic-inorganic hybrid thermoelectric materials possess numerous advantages, including functional aspects such as flexibility or transparency, low cost raw materials, and simplified fabrication processes, thus, allowing for a wide range of potential applications. In this study, the types and synthesis methods of organic-inorganic thermoelectric hybrid materials were discussed along with the methods used to enhance their thermoelectric properties. As a key factor to maximize the thermoelectric performances of hybrid thermoelectric materials, the nanoengineering to control the nanostructure of the inorganic materials as well as the modification of the organic material structure and doping level are considered, respectively. Meanwhile, the interface between the inorganic and organic phase is also important to develop the hybrid thermoelectric module with excellent reliability and high thermoelectric efficiency in addition to its performance in various electronic devices.

• 설비공학논문집
• /
• 제18권11호
• /
• pp.874-880
• /
• 2006

The power density and heat dissipation rate per unit area of the telecommunication equipment have been increased with the technology development in the footprint of telecommunication hardware. A proper heat dissipation method from the PCB module is very important to allow reliable operation of its electronic component. In this study, a hybrid air-conditioner for the telecommunication equipment room was designed to save energy and obtain system reliability. For high outdoor temperatures, the hybrid system operates in the vapor compression cycle, while, for low outdoor temperatures, the hybrid system works in the secondary fluid cooling cycle with no operation of the compressor. The performance of the hybrid air-conditioner was measured by varying outdoor and indoor temperatures. The hybrid air-conditioner yielded 50% energy saving compared with the conventional refrigeration system when the mode switch temperature was $8.3^{\circ}C$.

• ETRI Journal
• /
• 제34권6호
• /
• pp.838-846
• /
• 2012

This paper proposes a cost-effective hybrid-type power budget extender (PBEx) that can provide a high power budget of over 45 dB in an asymmetric 10-Gb/s Ethernet passive optical network (10/1G-EPON). The hybrid-type 10/1G-EPON PBEx comprises a central office terminal (COT) and remote terminal (RT) module supporting four channels and uses a coarse wavelength division multiplexing (CWDM) technology between the COT and RT for a reduction of fiber cost and efficient access network design. The proposed 10/1G-EPON PBEx can provide over a 40-km reach and 128-way split per CWDM wavelength with no modification of a legacy 10/1G-EPON system and can satisfy the error-free service in $10^{10}$ packet transmission.

• 한국신재생에너지학회:학술대회논문집
• /
• 한국신재생에너지학회 2010년도 춘계학술대회 초록집
• /
• pp.143.2-143.2
• /
• 2010

Therefore, with this development assignment we'd like to develop the hybrid system combining 800W DMFC (Direct Methanol Fuel Cell) and 1.6kW of Lithium secondary battery pack which can be applied to the most common small cart. a scooter, to secure the development capability of hundreds of Watts DMFC, the high-capacity Lithium secondary battery pack, the technology of BMS (Battery Management System) and the development technology of hybrid system. DMFC, in fact, has lower energy efficiency than PEMFC (Polymer Electrolyte Membrane Fuel Cell); however, it has several advantages in terms of fuel storage and use. It is pretty easy to be stored and used without any additional colling and heating devices because of its insensitive liquid methanol to temperature. In conclusion, DMFC system is the most suitable device for small mobile vehicles.

• Journal of Electrical Engineering and Technology
• /
• 제11권6호
• /
• pp.1684-1692
• /
• 2016

In order to adapt the fast dynamic performances of Buck DC/DC converter, and reduce the influence on converter performance owing to uncertain factors such as the disturbances of parameters and load, a control strategy based on two-dimensional hybrid cloud model is proposed. Firstly, two cloud models corresponding to the specific control inputs are determined by maximum determination approach, respectively, and then a control rule decided by the two cloud models is selected by a rule selector, finally, according to the reasoning structure of the rule, the control increment is calculated out by a two-dimensional hybrid cloud decision module. Both the simulation and experiment results show that the strategy can dramatically improve the dynamic performances of the converter, and enhance the adaptive ability to resist the random disturbances, and its control effect is superior to that of the current-mode control.

• 한국자동차공학회논문집
• /
• 제7권9호
• /
• pp.75-81
• /
• 1999

In this paper, the details of electronically controlled CVT is described , which Kia Motor company recently developed in prototype form for hybrid passenger car. This transmission has two input shafts, one for engine and the other for traction motor. The shaft for traction motor is located at rear side which is extended from primary pulley shaft and connected to traction motor through adapter gear box. Adopting two input system, various driving mode is available such as motor alone driving in hybrid vehicle application. As far as electrohydraulic system concerned , this transmission uses two bleed type variable force solenoids for line pressure and ratio control, and one on-off solenoid for clutch control. Another feature for this transmission is that oil pump for transmission is separate from CVT for supplying oil pressure even at vehicle standstill.

• 한국태양에너지학회 논문집
• /
• 제25권3호
• /
• pp.47-52
• /
• 2005

Normally if sunlight is directed on a solar cell without any increasing in temperature, the amount of absorption energy per unit area of each cell is increasing. In a silicon solar cell. however, cell conversion efficiency decreases with the increase of temperature. Therefore, to maintain cell conversion efficiency under normal condition, it is necessary to keep the cell at operating temperature. We tried to design and make new hybrid panel with cooling system to prevent increasing of temperature on cell, collect and use thermal energy more effectively. We compared performance of this new hybrid panel with current thermal panel. We also evaluated conversion efficiency, thermal capacity and confirmed cooling effects from thermal absorption efficiency.

• 한국지열·수열에너지학회논문집
• /
• 제2권2호
• /
• pp.1-8
• /
• 2006

The objective of the present study is to investigate the load delivery characteristics of a hybrid-renewable energy system with geothermal and solar heat sources for hot water, heating and cooling of a residential house in Korea. The hybrid energy system consists of ground source heat pump of 2 RT for cooling with a 150 m vertical U-bend ground heat exchanger, solar collectors of 4.8 m2 and gas fired backup boiler. The averaged coefficient of performance of geothermal module during cooling and heating seasons are evaluated as about 4.5 and 3.8, respectively.