• Biomolecules & Therapeutics
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• 제31권5호
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• pp.550-558
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• 2023

Hair loss is a common condition that can have a negative impact on an individual's quality of life. The severe side effects and the low efficacy of current hair loss medications create unmet needs in the field of hair loss treatment. Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1), one of the components of the extracellular matrix, has been shown to play a role in maintaining its integrity. HAPLN1 was examined for its ability to impact hair growth with less side effects than existing hair loss treatments. HAPLN1 was predominantly expressed in the anagen phase in three stages of the hair growth cycle in mice and promotes the proliferation of human hair matrix cells. Also, recombinant human HAPLN1 (rhHAPLN1) was shown to selectively increase the levels of transforming growth factor-β receptor II in human hair matrix cells. Furthermore, we observed concomitant activation of the ERK1/2 signaling pathway following treatment with rhHAPLN1. Our results indicate that rhHAPLN1 elicits its cell proliferation effect via the TGF-β2-induced ERK1/2 pathway. The prompt entering of the hair follicles into the anagen phase was observed in the rhHAPLN1-treated group, compared to the vehicle-treated group. Insights into the mechanism underlying such hair growth effects of HAPLN1 will provide a novel potential strategy for treating hair loss with much lower side effects than the current treatments.

• Journal of Microbiology and Biotechnology
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• 제34권3호
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• pp.506-515
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• 2024

Primary human dermal papilla cells (HDPCs) are often preferred in studies on hair growth and regeneration. However, primary HDPCs are limited by their reduced proliferative capacity, decreased hair induction potential, and extended doubling times at higher passages. To overcome these limitations, pTARGET vectors containing human papillomavirus16 (HPV16) E6/E7 oncogenes were transfected into HDPCs and selected using G-148 to generate immortalized cells here. HPV16 E6/E7 oncogenes were efficiently transfected into primary HDPCs. Immortalized HDPC showed higher proliferative activity than primary HDPC, confirming an increased proliferation rate. Expression of p53 and pRb proteins was downregulated by E6 and E7, respectively. E6/E7 expressing HDPC cells revealed that cyclin-dependent kinase (CDK) inhibitor p21 expression was decreased, while cell cycle-related genes and proteins (CDK2 and cyclin E) and E2F family genes were upregulated. Immortalized HDPCs maintained their responsiveness to Wnt/β-catenin pathway and hair follicle formation capability, as indicated by their aggregative properties and stemness. E6/E7 immortalized HDPCs may facilitate in vitro hair growth and regeneration studies.

• 한국생활과학회지
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• 제16권5호
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• pp.1051-1059
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• 2007

This study intends to find out possible variations in physical properties and color of hair depending on dye type(acidic and alkalic) and permanent treatment/hair dyeing procedure. To meet the goals, healthy hair samples were taken from 5 women's hair speciment in 20's without experiencing in any harmful substance via long-term medications. and beauty care using chemicals. To get possible findings depending upon hair dye type and treatment procedure, both acidic and alkalic hair dye were applied on some specimens after permanent treatment, while on other specimens before permanent treatment. In order to determine scale damage, this study used SEM(Hitachi S-2500C) both hair thickness and tensile strength were measured with optical microscope(Nikon, MM-60/L3T) and Instron (4482-standard). Wave configuration was measured transverse and longitudinal round diameter. The chromaticity of each specimen was measured using a spectrocolorimeter(Color Techno System, JP/JX-777) with visual $C/2^{\circ}$ (at $2^{\circ}$ with C light source) monitor fixed to determine Lab and CMYK values. As a result, it was found that hair specimens were more significantly damaged with alkalic hair dye treatment than with acidic hair dye treatment, while hair specimens were more effectively dyed with the former hair dye type than with the latter one. For possible results depending on treatment procedure, it was found that hair specimens were less damaged but more effectively dyed with permanent treatment followed by hair dyeing than vice versa. Therefore, it was concluded that permanent treatment followed by acidic hair dyeing would be more effective in reducing hair damages.

• 약학회지
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• 제47권3호
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• pp.142-147
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• 2003

Gas chromatography-mass spectrometric (GC-MS) procedure is presented for the simultaneous qualification and quantitation of methamphetamine (MA), amphetamine (AMP), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxyethylamphetamine (MDEA) in human hair. The method procedure involves decontamination of hair with distilled water and acetone, acidic hydrolysis, extraction in the presence of deuterated internal standards, and GC-MS analysis after derivatization with trifluoroacetic anhydride (TFAA) in ethylacetate. The limit of detection for 5 drugs were about 0.1∼0.15 ng/mg using 30 mg hair sample. Coefficient variations of correlation ranged from 0.9941 to 0.9993. The recoveries of these drugs were found to be 93.4∼104.4％. The concentrations of AMP, MA, MDA, and MDMA in abusers' hair samples were measured 0.17∼2.88, 2.09∼18.34, 0.24∼3.83, and 3.10∼22.81 ng/mg, respectively. The ratios of MA/AMP and MDMA/MDA ranged 5.67∼49.57 and 4.78∼54.31, respectively. This assay has been successfully utilized in the evaluation of the deposition of amphetamine-type stimulants (ATS) in human hair.

• Applied Microscopy
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• 제27권3호
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• pp.321-332
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• 1997

The dermal papilla is known to playa major role in influencing the form and dynamics of the hair follicle, which probably involves regulatory substances crossing the basal lamina. But little is known about the junctions between the dermal papilla and the surrounding epithelial cells of the hair bulb, or between the connective tissue and the epithelial cells on the outside of the hair follicle. This study was performed to identify the ultrastructural differences between dermoepidermal junction of the skin and connective tissue-epithelial junctions on the outside of the hair follicle and around the dermal papilla of normal anagen hair follicles in the human fetal scalp skin. Electron microscopic findings of dermoepidermal junction in scalp skin showed that basal lamina was very irregular and undulated, and it contained many attachment plaques of hemidesmosomes with sub-basal dense plates, tonofilaments, and anchoring filaments. Also invaginations of plasma membrane of basal keratinocytes were seen. There were clear differences both on the outside of the follicle and around the dermal papilla as compared with similar junction in the skin. In particular, neither hemidesmosomes nor tonofilaments, as seen in dermoepidermal junction, were observed in the dermal papilla. Also attachment plaque, sub-basal dense plate and anchoring filaments were not observed at the junction on the outside of the follicle and the dermal papilla. There were some differences between connective tissue-epithelial junctions on the outside of the hair follicle and around the dermal papilla, ie, smoothness of basal lamina and orthogonal arrangement of collagen fibers were seen in the outside of hair follicle, but not in the dermal papilla. These results indicate that the mechanical connection between the hair follicle and the connective tissue component is much weaker than that between the corresponding components in skin, and it reflects the dynamic processes during the anagen phase of the hair follicle compared to the relatively permanent state of the epidermis.

• Journal of Ginseng Research
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• 제47권3호
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• pp.440-447
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• 2023

Background: The human hair follicle undergoes cyclic phases-anagen, catagen, and telogen-throughout its lifetime. This cyclic transition has been studied as a target for treating hair loss. Recently, correlation between the inhibition of autophagy and acceleration of the catagen phase in human hair follicles was investigated. However, the role of autophagy in human dermal papilla cells (hDPCs), which is involved in the development and growth of hair follicles, is not known. We hypothesized that acceleration of hair catagen phase upon inhibition of autophagy is due to the downregulation of Wnt/β-catenin signaling in hDPCs, and that components of Panax ginseng extract can increase the autophagic flux in hDPCs. Methods: We generated an autophagy-inhibited condition using 3-methyladenine (3-MA), a specific autophagy inhibitor, and investigated the regulation of Wnt/β-catenin signaling using the luciferase reporter assay, qRT-PCR, and western blot analysis. In addition, cells were cotreated with ginsenoside Re and 3-MA and their roles in inhibiting autophagosome formation were investigated. Results: We found that the unstimulated anagen phase dermal papilla region expressed the autophagy marker, LC3. Transcription of Wnt-related genes and nuclear translocation of β-catenin were reduced after treatment of hDPCs with 3-MA. In addition, treatment with the combination of ginsenoside Re and 3-MA changed the Wnt activity and hair cycle by restoring autophagy. Conclusions: Our results suggest that autophagy inhibition in hDPCs accelerates the catagen phase by downregulating Wnt/β-catenin signaling. Furthermore, ginsenoside Re, which increased autophagy in hDPCs, could be useful for reducing hair loss caused by abnormal inhibition of autophagy.

• The Korean Journal of Physiology and Pharmacology
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• 제22권5호
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• pp.555-566
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• 2018

Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) are used in tissue repair and regeneration; however, the mechanisms involved are not well understood. We investigated the hair growth-promoting effects of hUCB-MSCs treatment to determine whether hUCB-MSCs enhance the promotion of hair growth. Furthermore, we attempted to identify the factors responsible for hair growth. The effects of hUCB-MSCs on hair growth were investigated in vivo, and hUCB-MSCs advanced anagen onset and hair follicle neogeneration. We found that hUCB-MSCs co-culture increased the viability and up-regulated hair induction-related proteins of human dermal papilla cells (hDPCs) in vitro. A growth factor antibody array revealed that secretory factors from hUCB-MSCs are related to hair growth. Insulin-like growth factor binding protein-1 (IGFBP-1) and vascular endothelial growth factor (VEGF) were increased in co-culture medium. Finally, we found that IGFBP-1, through the co-localization of an IGF-1 and IGFBP-1, had positive effects on cell viability; VEGF secretion; expression of alkaline phosphatase (ALP), CD133, and ${\beta}-catenin$; and formation of hDPCs 3D spheroids. Taken together, these data suggest that hUCB-MSCs promote hair growth via a paracrine mechanism.

• 한국HCI학회논문지
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• 제2권2호
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• pp.1-9
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• 2007

본 논문에서는 3차원 배우 모델링을 위해, 깊이 카메라를 이용하여 깊이 영상 획득 시 손실되는 머리카락 영역에 대한 복원기법을 제안한다. 대상 객체의 동적인 3차원 정보는 적외선 센서가 장착된 깊이 카메라를 통하여 실시간으로 획득한다. 이때, 깊이 비디오뿐만 아니라 각 프레임마다 컬러영상이 동시에 획득된다. 그러나 대상 객체의 일부 또는 전체가 반짝이면서 어두운 재질로 되어있을 경우 획득한 깊이 영상에서 그 부분 전체가 손실되는데, 이는 특히 방송용 콘텐츠로서 연기자의 3차원 정보를 획득할 때 머리카락 영역이 손실되어 매우 부자연스러운 결과를 초래한다. 이러한 문제점을 손실된 영역의 복원을 통해 해결한다. 먼저 컬러 영상을 이용하여 손실된 영역의 위치 정보를 알아내고, 손실된 영역 내 경계부분의 깊이 정보를 복원한 후 2차 베지어 커브로 보간하여 내부의 깊이 정보를 복원한다. 개선된 깊이 영상을 기반으로 일련의 모델링 과정을 수행하면 보다 자연스러운 3차원 모델을 생성할 수 있다. 생성된 3차원 모델은 실감방송용 콘텐츠로 사용될 수 있으며, 시청자에게 시각상호작용과 촉각상호작용 등 다차원 감각의 상호작용을 제공할 수 있다.

• 한방안이비인후피부과학회지
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• 제27권3호
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• pp.56-71
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• 2014

Objectives : This study was carried out to examine the change of scalp and hair condition and to evaluate the efficacy of hair growth, subject's satisfaction and safety of the Narasoo healing shampoo and Hair tonic Methods : We offered 25 subjects Narasoo healing shampoo and Hair tonic and observed the changes among the starting point, 3rd week and 6th week. Study methods include the measurement of water and sebum content of the scalp, photo of scalp and hair, thermographic images and questionnaires. Results : It is estimated that Narasoo healing shampoo and Hair tonic have the positive effects on increase of water contents hair gloss, removal of excessive hair keratin, facilitation of hair growth, and decrease of sebum contents. Conclusions : Several meaningful effects of Narasoo healing shampoo and Hair tonic are observed in this study. Additional research is needed forward.

• 패션비즈니스
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• 제17권3호
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• pp.48-57
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• 2013

This study was performed to quantitatively and qualitatively estimate the effect of keratin essence on hair protection against physicochemical damage. Damaged hairs were obtained from an early thirty woman who dyed her hair two times and did digital permanent treatment of her hair two times. The damaged hairs were divided into four experimental groups, which are the control hair (CH) group without additional beauty treatment, the damaged hair (DH) group by additional dyeing treatment, basic essence-treated hair (BEH) group, and keratin essence-treated hair (KEH) groups according to the research goal. The protection effect of keratin essence against the physicochemical damage was quantitatively compared by difference of chrominance measured using a color difference meter and qualitatively compared by difference of outer morphological structure images pictured using scanning electron microscopy (SEM). The brightness and yellowish blue color of KEH were relatively lower but the reddish blue color was relatively higher than other groups of test hairs. Cuticle structure of the previously DH was irregularly deformed and more strongly deformed or partially broken by additional dyeing treatment. On the other hand, the gaps between cuticle scales of the DH were reformed by treatment with basic essence and reformed and filled by treatment with keratin essence in comparison with the DH group. Conclusively, the keratin essence was effective to protect hair structure against the structural damage induced by the dyeing-treatment, by which the coloring efficiency is thought to be improved.