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http://dx.doi.org/10.4196/kjpp.2018.22.5.555

Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth  

Bak, Dong Ho (Department of Dermatology, College of Medicine, Chung-Ang University)
Choi, Mi Ji (Department of Dermatology, College of Medicine, Chung-Ang University)
Kim, Soon Re (Department of Dermatology, College of Medicine, Chung-Ang University)
Lee, Byung Chul (Department of Dermatology, College of Medicine, Chung-Ang University)
Kim, Jae Min (Department of Medicine, Graduate School, Chung-Ang University)
Jeon, Eun Su (Biomedical Research Institute, R&D Center, MEDIPOST Co., Ltd.)
Oh, Wonil (Biomedical Research Institute, R&D Center, MEDIPOST Co., Ltd.)
Lim, Ee Seok (Thema Dermatologic Clinic)
Park, Byung Cheol (Department of Dermatology, Dankook Medical College)
Kim, Moo Joong (Fort Hays State University)
Na, Jungtae (Department of Dermatology, College of Medicine, Chung-Ang University)
Kim, Beom Joon (Department of Dermatology, College of Medicine, Chung-Ang University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.22, no.5, 2018 , pp. 555-566 More about this Journal
Abstract
Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) are used in tissue repair and regeneration; however, the mechanisms involved are not well understood. We investigated the hair growth-promoting effects of hUCB-MSCs treatment to determine whether hUCB-MSCs enhance the promotion of hair growth. Furthermore, we attempted to identify the factors responsible for hair growth. The effects of hUCB-MSCs on hair growth were investigated in vivo, and hUCB-MSCs advanced anagen onset and hair follicle neogeneration. We found that hUCB-MSCs co-culture increased the viability and up-regulated hair induction-related proteins of human dermal papilla cells (hDPCs) in vitro. A growth factor antibody array revealed that secretory factors from hUCB-MSCs are related to hair growth. Insulin-like growth factor binding protein-1 (IGFBP-1) and vascular endothelial growth factor (VEGF) were increased in co-culture medium. Finally, we found that IGFBP-1, through the co-localization of an IGF-1 and IGFBP-1, had positive effects on cell viability; VEGF secretion; expression of alkaline phosphatase (ALP), CD133, and ${\beta}-catenin$; and formation of hDPCs 3D spheroids. Taken together, these data suggest that hUCB-MSCs promote hair growth via a paracrine mechanism.
Keywords
Alopecia; Dermal papilla cell; Hair growth; IGFBP-1; Stem cell; Stem-cell therapy;
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