• 한국미생물·생명공학회지
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• 제27권2호
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• pp.102-106
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• 1999

To investigate the effect of wormwood extract on intestinal Microorganisms of human, we examined the changes of the intestinal microflora of 6 volunteers for 13 days. Numbers of total anaerobes in feces of 6 volunteers showed 1010cfu/g before and after intake of wormwood extract. In case of beneficial Microorganisms, wormwood extract did not have an effect on the growth of bifidobacteria and Lactobacillus spp. On the other hand, the growth of C. perfringens and E. coli, the harmful Microorganisms of human intestines, was inhibited by the wormwood extract.

• Journal of Dairy Science and Biotechnology
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• 제31권2호
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• pp.153-159
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• 2013

유아 분변, 우분, 염소 분변, 개 분변, 돼지 분변, vaginal tract 채취물, 채소류, 부패한 과일류, 김치, 젓갈, 발효 소세지, 원유, 치즈, 발효유, 청국장, 메주, 막걸리 등 다양한 시료로부터 3,000주의 미생물을 분리하였다. 분리미생물은 L. monocytogenes, E. coli O157:H7, S. enterica serovar Enteritidis에 대해 항균활성을 측정하여 박테리오신 생산 미생물 26주를 최종 선발하였다. 16S rDNA gene sequencing 분석을 통하여 동정한 결과, Enterococcus faecalis(7주), E. faecium(8주), E. hirae(5주), Lactobacillus acidophilus(1주), L. amylovorus(1주), L. curvatus(2주), L. plantarum(1주), Pediococcus acidilactici(1주)와 같이 8종의 미생물이 박테리오신을 생산하는 것으로 확인되었다. 미생물이 생산한 박테리오신은 대부분이 단백질 또는 펩타이드성 물질이어서 인간 또는 동물의 소화효소에 의해 분해되므로 식품에서 안전한 천연보존제로 사용할 수 있을 것으로 기대된다.

• Natural Product Sciences
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• 제10권6호
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• pp.347-352
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• 2004

When saponin extracts of dried ginseng and red ginseng were anaerobically incubated with human intestinal microflora, these extracts were metabolized to compound K and ginsenoside $Rh_2$, respectively. However, when these extracts were incubated with commercial lactic acid bacteria, these did not metabolize these ginsenosides to compound K or ginsenoside $Rh_2$. Among some intestinal bacteria isolated from human feces, Bacteroides C-35 and C-36 transformed these saponin extracts to compound K and ginsenoside $Rh_2$, respectively. These bacteria also transformed water extracts of dried ginseng and red ginseng to compound K and ginsenoside $Rh_2$, respectively, similarly with that of the saponin extracts. Among transformed ginsenosides, compound K and 20(S)-ginsenoside $Rh_2$ exhibited the most potent cyotoxicity against tumor cells.

• 한국미생물·생명공학회지
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• 제22권4호
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• pp.443-445
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• 1994

Eubacterium is one of the predominant bacteria in the human large intestine. currently ES (Eubacterium Selective) medium developed by T. Mitsuoka is commonly used as a selective medium. neomycin sulfate which is one of the selective agents of ES medium inhibited about 50% of the growth of Eubacterium isolated, whereas malidixic acid inhibited only 5% while inhibiting other intestinal bacteria. NES medium which replaced neomycin with nalidixic acid in the ES medium was designed and shown to be better for the isolation and enumeration of Eubacterium sp. than ES medium.

• 한국식품영양과학회:학술대회논문집
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• 한국식품영양과학회 2001년도 International Symposium on Food,Nutrition and Health for 21st Century
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• pp.161-169
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• 2001

Lactic acid producing bacteria were isolated from baby feces and characterized to be used as a probiotic with anti Helicobacter pylori functions. The selected bacteria had inhibition activity on the adherance and growth of H. pylori. These bacteria had additional beneficial characteristics for the probiotic such as antibacterial activity, antitumor activity, immunostimulation activity, resistance to antibiotic and bile salt, ability to bind to the intestinal cells, and safe for the human use.

• 한국동물위생학회지
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• 제32권3호
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• pp.241-249
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• 2009

Shiga toxin (Stx)-producing Escherichia coli (STEC) strains can cause broad spectrum of human disease, including diarrhea, hemorrhagic colitis, and the life-threatening hemolytic uremic colitis (HUS). We examined 868 samples was taken from bovine feces and carcass from January to December 2008 in Seoul. Twenty two (9.5%) shiga toxin -producing Escherichia coli were isolated from the 230 of bovine feces, and two (0.31%) were isolated from the 638 of carcasses. Serotype of E. coli isolates were O157 (10, 41.6%), O26 (10, 41.6%), O111 (1, 4.2%) and UT (3, 12.6%). In PCR, the isolates displayed three different stx gene combination (stx1 [2, 8.4%]), stx2 [3, 12.6%] and stx1 and stx2 [19,87.5%]). The eaeA and hlyA gene were found in 11 (45.8%) of the 24 strains. Saa gene was present only one strains (4.2%). Toxin typing using reverse passive latex agglutination test showed the same result in VT 1. But it showed different result in VT 2. In antimicrobial susceptibility test, all isolates were sensitive to amikacin, amoxicillin/clavulanic acid, ciprofloxacin and colistin. Eighteen strains (75.0%) of 24 isolates showed the multi-resistant patterns with over 3 drugs. PFGE was performed after the genomic DNA of twenty four isolates was digested with Xba I. the 24 isolates showed 7 (A~G) PFGE type.

• Journal of Microbiology and Biotechnology
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• 제30권9호
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• pp.1321-1334
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• 2020

Beef, pork, chicken and milk are considered representative protein sources in the human diet. Since the digestion of protein is important, the role of intestinal microflora is also important. Despite this, the pure effects of meat and milk intake on the microbiome are yet to be fully elucidated. To evaluate the effect of beef, pork, chicken and milk on intestinal microflora, we observed changes in the microbiome in response to different types of dietary animal proteins in vitro. Feces were collected from five 6-week-old pigs. The suspensions were pooled and inoculated into four different media containing beef, pork, chicken, or skim milk powder in distilled water. Changes in microbial communities were analyzed using 16S rRNA sequencing. The feces alone had the highest microbial alpha diversity. Among the treatment groups, beef showed the highest microbial diversity, followed by pork, chicken, and milk. The three dominant phyla were Proteobacteria, Firmicutes, and Bacteroidetes in all the groups. The most abundant genera in beef, pork, and chicken were Rummeliibacillus, Clostridium, and Phascolarctobacterium, whereas milk was enriched with Streptococcus, Lactobacillus, and Enterococcus. Aerobic bacteria decreased while anaerobic and facultative anaerobic bacteria increased in protein-rich nutrients. Functional gene groups were found to be over-represented in protein-rich nutrients. Our results provide baseline information for understanding the roles of dietary animal proteins in reshaping the gut microbiome. Furthermore, growth-promotion by specific species/genus may be used as a cultivation tool for uncultured gut microorganisms.

• 한국잠사곤충학회지
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• 제53권2호
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• pp.78-81
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• 2015

본 연구는 사육용 쌍별귀뚜라미의 배변제거를 위한 절식시간을 알아보는 것을 실험 목적으로 하였다. 귀뚜라미는 현재 드디어 한시적 식품인정을 받았다. 따라서 식용 곤충으로서 국내시판을 위한 연구의 일환으로 귀뚜라미 생체 안의 배변을 제거하여 미생물의 번식을 예방하고 이취 제거와 맛을 향상시키기 위하여 본 쌍별귀뚜라미의 절식 실험을 실시한 결과 농가와 실험실에서 두 곳 모두 절식 2일째부터는 귀뚜라미의 습성에 의해 동족포식의 카니발리즘이 발동하여 귀뚜라미의 생체가 훼손되어 나감을 관찰 하였다. 쌍별 귀뚜라미 장내 분변을 현미경으로 살펴본 결과 1일째 이후 분변은 거의 없어지고 싸움으로 획득한 귀뚜라미의 팔다리 일부가 장내에 들어 있는 양상을 보여 1일이 귀뚜라미 장내 변이 거의 없어지는 시간임을 알 수 있어 생체 운송 배달시 1일이 걸리는 기간을 감안하면 절식시간은 1일이 적당하다고 사려 되어진다.

• Parasites, Hosts and Diseases
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• 제47권3호
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• pp.315-318
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• 2009

Quantitative fecal egg counts represented as the number of eggs per gram of feces (EPG) are generally a reliable parameter to estimate the worm burden of intestinal and hepatic parasitoses. Although Haplorchis taichui (Digenea: Heterophyidae) is one of the most common minute human intestinal flukes, little is known about the relationship between EPG and the actual worm burden in patients or the severity of the disease. In the present study, fecal samples were collected from 25 villagers in northern Thailand before and after praziquantel treatment. The EPG values of each participant were determined by the modified cellophane thick smear method, and adult worms were collected from the whole stool after the treatment. Eggs per day per worm (EPDPW) of H. taichui were estimated 82 from egg counts and expelled worms. The EPG was not well correlated with the worm burden, and a reverse correlation was observed between the EPDPW and the worm burden.

• 한국동물위생학회지
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• 제24권2호
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• pp.133-138
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• 2001

Cryposporidium species have a wide host range. These coccidian parasites are found in close association with epithelial cells of many species of animals including mm. The gastrointestinal tract is most commonly affected in young ruminants and this parasite is thought to be considerable importance in calf diarrhea complex. Major outbreaks of cryptosporidiosis have been reported in calves, lambs, pigs and others including avian species. Cryptosporidiosis is transmitted by oocysts of Cryptosporidium species. Because cryptosporidiosis is common infection among animals, early literature considered it a zoonosis. Human infections contracted from calves, cats, and horse feces. However, the resrvoir host is longer considered the major source of infection. Mild cases of disease have been reported in farm workers. Immunosuppressed, very young and very old persons should avoid contact with this parasite because it may cause severe diarrhea. In order to detect of Crytosporidium sp infection from feces of cattle and pigs at Chonbuk Iksan and Kunsan area, sedimentation and modified acid fast stain were applied. The positive rate of Cryptosporidium sp infection from 1, 176 of cattle and 267 of pigs were 0.5 % and 16.8%, respectively. According to area in Iksan and Kunsan, the positive rates were 0.4% and 0.9% from cattle, and 18.9% and 12.1% from pigs, respectively. In cattle, positive detection rate was 0.6% in milking cows but not in Korean cattle.