• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.1
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• pp.86-92
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• 2007

Hibiscus sabdariffa L., a tropical beverage material, is used commonly as in folk medicine against hypertension, pyrexia, inflammation, liver disorders, and obesity. However, the mechanism by which Hibiscus sabdariffa L. modulates adipogenic differentiation is remained to be elusive. This report was designed to investigate the inhibitory effect of Hibiscus extract on insulin signaling pathway during adipocyte differentiation in 3T3-L1 preadipocytes. 3T3-L1 preadipocytes were differentiated with isobutylmethylxanthine, dexamethasone, and insulin (MDI) and followed by the addition of Hibiscus extract. Treatment with Hibiscus resulted in a decrease of lipid droplet accumulation, which was suppressed by PI-3 kinase inhibitor wortmannin in 3T3-L1 preadipocytes. Also, Hibiscus extract markedly attenuated the mRNA expression of adipogenic transcriptional factor PPAR${\gamma}$ and adipogenic hormon Leptin during adipogenesis. However, it did not affect the expression of adiponectin in 3T3-L1 preadipocytes differentiated with MDI mixture. Furthermore, Adipogenic differentiation by MDI mixture increased the phosphorylation and expression of PI3-Kinase and Akt in 3T3 preadipocytes, which was markedly suppressed by Hibiscus extract treatment. Taken together, our results suggest that Hibiscus extract suppressed the adipogenic differentiation of 3T3 preadipocytes through activation of PI3-Kinase and Akt signaling pathway.

• Journal of Life Science
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• v.27 no.7
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• pp.790-795
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• 2017

The purpose of this study was to evaluate the anti-oxidative effects of fermented Hibiscus syriacus extract on obese mice. The antioxidant experiments were conducted both in vivo and in vitro. The in vitro experiment was measured through the use of nitric oxide (NO) free radical scavenging activity. The results showed that the scavenging activity of the NO free radical in fermented Hibiscus syriacus extract had higher activity than unfermented Hibiscus syriacus extract. The in vivo experiments were divided into a normal dietary group, a high fat dietary group (CON), a high fat dietary + fermented Hibiscus syriacus extract group (FH), and a high fat dietary + unfermented Hibiscus syriacus extract group. These four groups received intraperitoneal administrations for four weeks. Next, the levels of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in a liver homogenate were measured. The results showed that the levels of SOD and CAT in FH were higher than the CON and MDA levels, which were significantly decreased in FH. Consequently, fermented Hibiscus syriacus extract had an inhibitive effect on oxidative stress and a heightening effect on antioxidant enzyme activity in obese mice, suggesting that fermented Hibiscus syriacus extract could be utilized as a useful, natural antioxidant.

• Journal of Applied Biological Chemistry
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• v.63 no.1
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• pp.61-67
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• 2020

Thirty-five edible plants were tested against oral bacteria (Streptococcus mutans, Lactobacillus casei, Staphylococcus epidermidis, Klebsiella pneumonia). The edible plants were extracted using 70% ethanol as a solvent. Among the thirty-five edible plants, hibiscus (Hibiscus sabdariffa) extract was selected as an effective antibacterial source because it showed the lowest minimum inhibitory concentration. The antimicrobial substances of hibiscus extract were very stable at acidic condition and at wide range of temperature 4-75 ℃. The total polyphenol and flavonoid contents of hibiscus extract were 130.5 and 25.7 mg/g, respectively. Also hibiscus extract showed a high degree of antioxidant activity.

• Fashion & Textile Research Journal
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• v.17 no.3
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• pp.476-485
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• 2015

The purpose of this research is to analyze the effects of persimmon juice and hibiscus flowers extract to perform combination dyeing of silk fabrics, with respect to color changes, fastness, and functionality. The optimal conditions as a result, were shown at 100% of dye concentration, $40^{\circ}C$ of dyeing temperature and 30 minutes of dyeing time while in terms of dye uptake depending on the kind of mordants and methods. The highest K/S value was recorded at pH 3, pre-mordants of $CuSO_4$, and $SnCl_2$, post-mordanting. After repeated dyeing and post-mordanting, various color change occurred with mordant treatments. Color fastness of dyed fabrics with hibiscus flowers extract was improve considerably by combination dyeing with persimmon juice extract. In aspects of functional properties, it showed excellent results of 99% deodorization rate, 99.9% Staphylococcus aureus rate and 99.9% Klebsiella pneumoniae.

• Journal of the Korean Society of Food Culture
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• v.32 no.4
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• pp.295-302
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• 2017

The aim of this study was to analyze the functional components of five cultivars of 'Mugunghwa (Hibiscus syriacus L.)' for the development of 'Mugunghwa'-added foods. There were five varieties, namely, Hibiscus syriacus 'Hwanhee' (HH), Hibiscus syriacus 'Samchulli' (SC), Hibiscus syriacus 'Harmony' (HM), Hibiscus syriacus 'Baekgu' (BG), and Hibiscus sabdariffa L (HB). Of the five varieties tested, HH extract showed the highest total phenolic content (131.85 GAE mg/g), DPPH free radical scavenging activity (95.18%), and anthocyanin and sugar contents. The sensory quality of powders from each Hibiscus variety was evaluated, and it was found that overall palatability decreased in the order: HH, SC, HB, HM, and BG. Thus, addition of Hibiscus variety, which has excellent functionality and palatability, to foods confers an extra element of Korean history and culture, which is expected to improve the marketability of various food products.

• Journal of the Korean Applied Science and Technology
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• v.41 no.2
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• pp.386-392
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• 2024

Due to COVID-19, modern people have come to prefer natural substances as anxiety due to harmful environments and various stimuli has increased. Therefore, in order to find out the appropriateness of hibiscus, which is recognized as a non-toxic plant in traditional medicine, as a natural cosmetic material, the antioxidant effects (polyphenol, flavonoid, DPPH, ABTS) of hibiscus extract were studied, and the following results were obtained. First, the total polyphenol of hibiscus was found to be 433 ㎍/mL when the HSE was 100%. Second, the total flavonoids showed high antioxidant capacity at 488 ㎍/mL in 100% of the HSE. Third, the DPPH radical scavenging ability was found to be 94.04% in the undiluted HES and 89.54% in the diluted HSE 20%. Fourth, the ABTS radical scavenging ability was 98.95% in 100% of the HSE stock solution and 94.84% in the diluted HSE 20%, respectively, showing a high scavenging ability of more than 90%. As a result of these studies, it is thought that the hibiscus extract can be used as an antioxidant raw material for natural cosmetics in the future.

• Food Science and Biotechnology
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• v.15 no.2
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• pp.260-263
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• 2006

In this study, we investigated the antimicrobial effect of 14 different herbal petal extracts on various foodborne and food spoilage bacteria. Herbal petal extracts were prepared with 70% ethanol followed by sequential hexane, chloroform, ethyl acetate, n-butanol, and water fractionation. Antimicrobial activity was highest in the ethanol fraction from roselle (Hibiscus sabdariffa L.) petals as determined by the paper disc method. The roselle ethanol extract retarded the growth of food spoilage bacteria in kimbap (rice rolled in dried laver). Foodborne microorganisms (e.g. Bacillus cereus and Clostridium perfringens), on the other hand, were most efficiently inhibited by the ethyl acetate fraction of the roselle petal extract as determined by growth inhibition curves. Our study shows that roselle petals harbor antimicrobial activity against foodborne and food spoilage microorganisms. The critical ingredient is highly enriched in the ethyl acetate fraction of the extract.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.3
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• pp.797-807
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• 2004

This research investigates the effect of the Hibiscus syriacus(HSS) on Alzheimer's disease. The effects of the HSS extract on the behavior in the Morris water maze experiment; the expression of IL-1β, TNF-α, IL-1β mRNA, TNF-α mRNA, CD68/GFAP and RDS; the the infarction area of the hippocampus, and brain tissue injury in Alzheimer's diseased mice induced with M were investigated. The HSS extract group showed a significant inhibitory effect on the memory deficit on the mice with Alzheimer's disease induced by βA in the Morris water maze experiment. The HSS extract group suppressed the over-expression of IL-1β, TNF-α, IL-1β and TNF-α mRNA, CD68/GFAP, RDS in the mice with Alzheimer's disease induced by βA. The HSS extract reduced the infarction area of hippocampus, and controlled the injury of brain tissue in the mice with Alzheimer's disease induced by βA. This study suggest that HSS may be effective for the prevention and treatment of Alzheimer's disease

• YAKHAK HOEJI
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• v.54 no.3
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• pp.164-167
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• 2010

Column chromatographic separation of the MeOH extract from the aerial parts of Hibiscus manihot led to the isolation of four compounds. Their structures were characterized to be hentriacontane (1), palmitic acid (2), daucosterol (3) and 3-dihydrocaffeonyl-5-p-coumaroylquinic acid (4) by spectroscopic methods. The compounds (1~4) were for the first time reported from this plant. The solvent fractions were tested for their antioxidant activities by free radical scavenging and superoxide dismutase (SOD).

• Food Science and Biotechnology
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• v.16 no.3
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• pp.409-414
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• 2007

Antimicrobial and antioxidative effects of 14 different herbal flower extracts on skin microorganisms and oxidation were tested in this research. Herbal flower extracts were prepared with 70% ethanol. Among the herbal flower extracts, roselle (Hibiscus sabdariffa L.) flower extract showed the highest antimicrobial activity against Staphylococcus epidermidis as determined by a paper disc method. The seventy % ethanol extract of roselle flower was fractionated by sequential hexane, chloroform, ethyl acetate, n-butanol, and water fractionation. The growth of S. epidermidis, Streptomyces collinus, Streptomyces coeruleoprunus, Salmonella enteritidis, Vibrio parahaemolyticus, and Malassezia pachydermatis was most efficiently inhibited by ethyl acetate fraction of roselle flower extract as determined by a paper disc method and growth inhibition curves. In addition, the ethyl acetate fraction, water fraction and butanol fraction showed free radical scavenging and DNA cleavage inhibition activities. These results demonstrate that roselle flowers hold antimicrobial and antioxidative activities against skin microorganisms and oxidants.