Browse > Article
http://dx.doi.org/10.5352/JLS.2017.27.7.790

The Anti-oxidative Activities of Fermented Hibiscus syriacus Extract in High Fat Diet-induced obese C57BL/6 Mice  

Lee, Ha Neul (Department of Pharmaceutical Engineering and College of Medical Life Science, Silla University)
Ha, Bae Jin (Department of Pharmaceutical Engineering and College of Medical Life Science, Silla University)
Publication Information
Journal of Life Science / v.27, no.7, 2017 , pp. 790-795 More about this Journal
Abstract
The purpose of this study was to evaluate the anti-oxidative effects of fermented Hibiscus syriacus extract on obese mice. The antioxidant experiments were conducted both in vivo and in vitro. The in vitro experiment was measured through the use of nitric oxide (NO) free radical scavenging activity. The results showed that the scavenging activity of the NO free radical in fermented Hibiscus syriacus extract had higher activity than unfermented Hibiscus syriacus extract. The in vivo experiments were divided into a normal dietary group, a high fat dietary group (CON), a high fat dietary + fermented Hibiscus syriacus extract group (FH), and a high fat dietary + unfermented Hibiscus syriacus extract group. These four groups received intraperitoneal administrations for four weeks. Next, the levels of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in a liver homogenate were measured. The results showed that the levels of SOD and CAT in FH were higher than the CON and MDA levels, which were significantly decreased in FH. Consequently, fermented Hibiscus syriacus extract had an inhibitive effect on oxidative stress and a heightening effect on antioxidant enzyme activity in obese mice, suggesting that fermented Hibiscus syriacus extract could be utilized as a useful, natural antioxidant.
Keywords
Antioxidative activities; fermentation; Hibiscus syriacus;
Citations & Related Records
Times Cited By KSCI : 15  (Citation Analysis)
연도 인용수 순위
1 Kobayashi, M., Fujita, M. and Mitsuhashi, H. 1984. Components of Cnidium officinale Makino: occurrence of pregnenolone, coniferylferulate, ferulate and hydroxylprthalides. Chem. Pharm. Bull. 32, 3770-3773.   DOI
2 Lee, E. J and Bae, J. H. 2011. Study on the alleviation of an alcohol induced hangover and the antioxidant activity by mulberry fruit. Kor. J. Food Nutr. 24, 204-209.   DOI
3 Lee, E. J. 2014. Effect of a pumpkin, corn silk, and adzuki bean on antioxidant activity and anti-obesity in diet-induced obese rat. M.S. dissertation, Kyungnam university, Kyungnam, Korea.
4 Lee, H. J., Lee, S. W., Park, C. G., Ahn, Y. S., Kim, J. S., Bang, M. S., Oh, C. H. and Kim, C. T. 2015. Effects of white Habiscus syriacus L. flower extracts on antioxidant activity and bone resorption inhibition. Kor. J. Med. Crop Sci. 23, 190-197.   DOI
5 Lee, J. J., Son, H. Y., Choi, Y. M., Cho, J. H., Min, J. K. and Oh, H. K. 2016. Physicochemical components and antioxidant activity of Sparassis crispa mixture fermented by lactic acid bacteria. Kor. J. Food Preserv. 23, 361-368.   DOI
6 Lee, S. J. 2016. Bioactive substances searching and bioactiviteis evalution of fermented Sargassum siliquanstrum. M.S. dissertation, Silla University, Busan, Korea.
7 Lim, K. T. and Shim, J. H. 1997. Antioxidative effects of ethanol extracts from Rhus Verniciflua Stokes (RVS) on mouse whole brain cells. Kor. J. Food Sci. Technol. 29, 1248-1254.
8 Marcocci, L., Packer, L., Dory-Lefaix, M. T., Sakaki, A. and Gerdes-Albert, M. 1994. Antioxidation action of Ginkgo biloba extract egb 76. Methods Enz. 234, 462-475.
9 Park, S. J., Kim, D. H., Paek, N. S. and Kim, S. S. 2006. Preparation and quality characteristics of the fermentation product of ginseng by lactic acid bacteria (FGL). J. Ginseng Res. 30, 88-94.   DOI
10 Aebi, H. 1984. Catalase in vitro, in : Packer. L , ed methods in enzymology. N. Y. Academic. 105, 121-126.
11 Bae, G. J. and Ha, B. J. 2015. Antioxidative effect of fermented Rhynchosia nulubilis in obese rats. J. Fd Hyg. Safety 30, 383-389.   DOI
12 Beak, Y. H. and Lee, S. H. 2010. The effect of aerobic exercise and Allium Tuberosum intake on blood lipids, MDA and antioxidant enzyme in rats. J. Life Sci. 20, 245-252.   DOI
13 Beauchamp, C. and Fridovich, I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44, 276-287.   DOI
14 Ha, B. J. 1985. Studies on the antilipidperoxidation effect of brazilin and hematoxylin. Ph. D. Dissertation. Seoul university, Seoul, Korea.
15 Ha, J. H., Seo, Y. C., Choi, W. Y., Kim, J. S., Kim, H. H., Ahn, J. H and Lee, H. Y. 2010. Enhancement of antioxidant activities of bark of Berberis koreana Palibin by lactic acid fermentation. Kor. J. Med. Crop Sci. 18, 421-428.
16 Jang, A. R. 2012. Study on cosmetic physiological and skin cell activity of Hibiscus syriacus extracts. M.S. dissertation, Hannam University, Daejeon, Korea.
17 Kim, J. D., Lee, B. I., Jeon, Y. H., Bak, J. P., Jin, H. R. and Lim, B. O. 2010. Anti-oxidative and anti-inflammatory effects of green tea mixture and dietary fiber on liver of high fat diet-induced obese rats. Kor. J. Med. Crop Sci. 18, 224-230.
18 Jeon, J. M., Choi, S. K., Kim, Y. J., Jang, S. J., Cheon, J. W. and Lee, H. S. 2011. Antioxidant and antiaging effect of ginseng berry extract fermented by lactic acid bacteria. J. Soc. Cosmet. Scientists Korea 37, 75-81.
19 Jung, Y. J. and Choi, M. J. 2015. Effects of chunggukjang and greentea-chunggukjang on lipid profile and antioxidative enzyme activity of liver tissue in growing rats fed cholesterol. J. East Asian Soc. Dietary Life 25, 278-286.   DOI
20 Kang, H. J., Kim, H. S., Jeon, I. H., Mok, J. Y., Han, K. S. and Jang, S. I. 2013. Effects of antioxidant and blood flow improvement of grape leaf extract and resveratrol from Vitis romaneti. J. Kor. Soc Food Sci Nutr. 42, 1736-1743.   DOI
21 Kim, J. H., Lee, W. J., Cho, Y. W. and Kim, K. Y. 2009. Storage-life and palatability extension of Betula platyphylla Sap using lactic acid bacteria fermentation. J. Kor. Soc. Food Sci. Nutr. 38, 787-794.   DOI
22 Kim, J. Y., Shin, M. S. and Heo, Y. R. 2014. Effects of stabilized rice bran on obesity and antioxidative enzyme activity in high fat diet-induced obese C57BL/6 mice. J. Kor. Soc. Food Sci. Nutr. 43, 1148-1157.   DOI
23 Yoo, K. O., Lim, H. T. and Kim, J. H. 1996. Studies on the flavonoids of the Hibiscus syriacus L. complex. Kor. J. Plant. Res. 9, 224-229.
24 Kim, M. A. 2014. Influences of feral haw (Crataegus pinnatifida BUNGE) on the antioxidative activities and lipid metabolism functional improvement in dyslipidemic rats. M.S. dissertation, Busan University, Busan, Korea.
25 Kim, S. M., Kim, D. Y., Park, H. R., Seo, J. H., Yeom, B. M., Jin, Y. J. and Pyo, Y. H. 2014. Screening the antioxidant components and antioxidant activitiy of extracts derived from five varieties of edible spring flowers. Kor. J. Food Sci. Technol. 46, 13-18.   DOI
26 Kim, S. W., Jeong, S. T. and Baek, Y. H. 2013. Effects of cheonggukjang diet and aerobic exercise on lipid metabolism and antioxidant enzyme in rats. J. Life Sci. 23, 657-663.   DOI
27 Sung, K. S., Chun, C., Kwon, Y. H., Kim, K. H. and Chang, C. C. 2000. Effects of red ginseng component on the antioxidative enzymes activities and lipid peroxidation in the liver of mice. J. Ginseng Res. 24, 29-34.
28 Yoo, I. D., Lee, I. K., Ryoo, I. J., Choung, D. H., Han, K. H. and Yun, B. S. 1997. Studies on the constituents of Hibiscus syriacus (I). Kor. J. Pharmacoan. 28, 112-116.
29 Yoon, S. W. 2016. Effect of absolute from Hibiscus syriacus L. flower on skin regeneration and wound healing in keratinocyte. M.S. dissertation, Hoseo University, Chungnam, Korea.