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Hibiscus Sabdariffa L. Inhibits the Insulin Signaling in Adipogenesis  

Kim, Jin-Kyung (Vestibulocochlear Research Center & Department of Microbiology, Wonkwang University)
Song, Jeong-Hoon (Plastic & Reconstructive Surgery, Wonkwang University School of Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.1, 2007 , pp. 86-92 More about this Journal
Abstract
Hibiscus sabdariffa L., a tropical beverage material, is used commonly as in folk medicine against hypertension, pyrexia, inflammation, liver disorders, and obesity. However, the mechanism by which Hibiscus sabdariffa L. modulates adipogenic differentiation is remained to be elusive. This report was designed to investigate the inhibitory effect of Hibiscus extract on insulin signaling pathway during adipocyte differentiation in 3T3-L1 preadipocytes. 3T3-L1 preadipocytes were differentiated with isobutylmethylxanthine, dexamethasone, and insulin (MDI) and followed by the addition of Hibiscus extract. Treatment with Hibiscus resulted in a decrease of lipid droplet accumulation, which was suppressed by PI-3 kinase inhibitor wortmannin in 3T3-L1 preadipocytes. Also, Hibiscus extract markedly attenuated the mRNA expression of adipogenic transcriptional factor PPAR${\gamma}$ and adipogenic hormon Leptin during adipogenesis. However, it did not affect the expression of adiponectin in 3T3-L1 preadipocytes differentiated with MDI mixture. Furthermore, Adipogenic differentiation by MDI mixture increased the phosphorylation and expression of PI3-Kinase and Akt in 3T3 preadipocytes, which was markedly suppressed by Hibiscus extract treatment. Taken together, our results suggest that Hibiscus extract suppressed the adipogenic differentiation of 3T3 preadipocytes through activation of PI3-Kinase and Akt signaling pathway.
Keywords
Hibiscus Sabdariffa L.; Insulin; isobutylmethylxanthine; dexamethasone;
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1 Wolf, A.M., Colditz, G.A. Social and economic effects of body weight in the United States. Am. J. Clin. Nutr. 63: 466S-469S, 1996   DOI
2 Pi-Sunyer, F.X. Medical hazards of obesity. Ann Intern Med. 19(7 Pt 2):655-660, 1993
3 Swen, W., Ying, W., Frank, T. Anti-obesity effects of green tea: from beside to bench. Mol. Nutr. Food Res. 50: 176-187, 2006   DOI   ScienceOn
4 El-saadany, S.S., Sitohy, M. Z., Labib, S.M. Biochemical dynamics and hypo cholesterolemic action of Hibiscus sabdariffa (Karkade). Nahrung. 35:567-576, 1991   DOI   ScienceOn
5 Rosen, E.D., Hsu, C.H., Wang, X., Sakai, S., Freeman, M. W., Gonzalez, F.J., Spiegelman. C/EBPalpha induces adipogenesis through PPARgamma: a unified pathway. Genes Dev. 16(1):22-26, 2002   DOI   ScienceOn
6 Johnson, P.F., Landschulz, W.H., Graves, B.J., McKnight, S.L. Identification of a rat liver nuclear protein that binds to the enhancer core element of three anival viruses. Gene Dev. 1:133-146, 1987   DOI   ScienceOn
7 Fiedman, J.M., Halaas, J.L. Leptin and the regulation of body weight in mammals. nature. 395:763-770, 1998   DOI   ScienceOn
8 Bluher, M., Patti, M.E., Gesta, S., Kahn, B.B., Kahn, C.R. Intrinsic heterogeneity in adipose tissue of fat-specific insulin receptor knockout mice is associated with differences in patterns of gene expression, J. Biol. Chem. 2004
9 Birkenmeier, E.H., Gwynn, B., Howard, S., Jerry, J., Gordon, J.I. Landschulz, W.H., McKnight, S.L. Tissue-specific expression, developmental regulation, and genetic mapping of the gene encoding CCAAT/enhancer binding protein. Genes Dev. 3(8):1146-1156, 1989   DOI   ScienceOn
10 Smith, P.J., Wise, L.S., Berkowit, R.C., Rubin, C.S. Insuli-like growth factor-I is an essential regulatior of the differentiation of 3T3-L1 adipocytes. J Biol Chem. 263:9402-9408, 1988
11 He, Y, Chen, H., Quon, M.J., Reitman, M. The mouse obese gene. Genomic organization, promoter activity, and activation by CCAAT /enhancer-binding protein alpha. J Biol Chem. 270:28887-28891, 1995   DOI
12 Haji, Raraji, Haji, Takhani. The effect of sour tea (Hibiscus sabdariffa L) on essential hypertension. 65:231-236, 1999
13 Onyenekwe, P.C., Ajani, E.O., Ameh, D.A., Gamaniel, K.S. Antihypertensive effect of roselle (Hibiscus sabdariffa) calyx infusion in spontaneously hypertensive rats and a comparison of its toxicity with that in Wistar rats. Cell Biochem Funct. 17(3):199-206, 1999   DOI   ScienceOn
14 Kuczmarski, R.J., Flegal, K.M., Campbell, S.M., Johnson, C.L. Increasing prevalence of overweight among US adults. The National Health and Nutrition Examination Sureveys. 1960 to 1991. JAMA. 272:205-211, 1994   DOI   ScienceOn
15 Update: Prevalence of overweight among children, adolescents, and adults-United States. Morb Mortal Wkly Rep. 46: 199-202, 1997
16 Hansawasdi, C., Kawabata, J., Kasai, T. Alpha-amylase inhibitors from roselle (Hibiscus sabdariffa Linn.) tea. Biosci Biotechnol Biochem. 64(5):1041-1043, 2000   DOI   ScienceOn
17 Cornelius, P., MacDounald, O.A,, Lane, M.D. Regulation of adipocyte development. Annu Rev Nutr. 14:99-129, 1994   DOI   ScienceOn
18 Czech, M.P. Fat targets for insulin signaling. Mol Cell. 9: 695-696, 2002   DOI   ScienceOn
19 Anne, W. Harmon, Joyce, B. Harp. Differentiatial effects of flavonoids on 3T3-L1 adipogenesis and lipolysis. Am J Physiol Cell Physiol. 280:C807-C813, 2001   DOI
20 McKnight, S.L. Transcriptional regulation. cold spring Harbor Lab. Press, Plainview. 771-795. 1992
21 Cheatham, B., Kahn, C.R. Insulin action and the insulin signaling network. Endocr Rev. 16:117-142, 1995
22 Farombi, E.O., Fakoya, A. Free radical scavenging and antigenotoxic activities of natural phenolic compounds in dried flowers. Mol Nutr Food Res. 49(12):1120-1128, 2005   DOI   ScienceOn
23 Sheard, N.F. Moderate changes in weight and physical activity can prevent or delay the development of type 2 diabetes mellitus in susceptible individuals. Nutr. Rev. 61: 76-79, 2003   DOI   ScienceOn
24 Regina, P.B., Kim, J.B., Erding, H., Soner, A., Bruce, M.S. Adipocyte differentiation: a transcriptional regulatory cascade. 8:826-832, 1996   DOI   ScienceOn
25 Bluher, M., Michael, M.D., Peroni, O.D., Ueki, K., Carter, N., Kahn, B.B., Kahn, C.R. Adipose tissue selective insulin receptor knockout protects against obesity and obesity-related glucose intolerance. Dev. Cell 3:25-38, 2002   DOI   ScienceOn
26 Lin, F.T., Lane, M.D. CCAAT/enhancer binding protein alpha is sufficient to initiate the 3T3- L1 adipocyte differentiation program. Proc Natl Acad Sci U S A. 91(19):8757-8761, 1994
27 Hirunpanich, V., Utaipat, A., Morales, N.P,, Bunyapraphatsara, N., Sato, H., Herunsale, A., Suthisisang, C. Hypocholesterolemic and antioxidant effects of aqueous extracts from the dried calyx of Hibiscus sabdariffa L in hypercholesterolemic rats. J Ethnopharmacol. 103(2):252-260, 2006   DOI   ScienceOn