• 제목/요약/키워드: herbal raw material

검색결과 60건 처리시간 0.026초

중약주사제 실태현황 분석을 통한 국내 약침 연구 방향 모색 (A Study on the Direction of Research for Pharmacopuncture through the Analysis on the Current Status of Chinese Herbal Injections)

  • 황지혜;최수현;송호섭
    • Korean Journal of Acupuncture
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    • 제38권4호
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    • pp.250-266
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    • 2021
  • Objectives : This study was conducted to suggest new alternative methods to improve pharmacopuncture and Korean medicine research by analyzing the injection route, pharmacological effect, and status studies of Chinese herbal injections. Methods : 130 types of marketed and licensed Chinese herbal injection were searched from National Medical Products Administration (NMPA) of China. CNKI, PubMed, EMBASE, and the 2020 edition of the Chinese Pharmacopoeia were used to collect additional information. 'Herbal injection' and 'Chinese herbal injection' were used as keywords. All data were collected mainly on the treatment of Chinese herbal injection. But data which were not related to the relevant research or Chinese herbal injection were excluded. Results : Intramuscular injection accounted for more than half of the single injection route (51%). Acupoint and intramuscular injections accounted for 55% of dual injection routes. Acupoint, intravenous, and intramuscular injections accounted for the largest proportion (76%) of the multiple routes of injections. As for the pharmacological effect, injection for cardiovascular diseases accounted for 29%. About the number of raw herbal materials, single herbal material was the most common. Twelve intervention studies all tested intravenous injections, and half of them investigated cardiocerebrovascular diseases. All were given by intravenous injection. In the side effect section, the most common symptoms were nausea and vomiting. Conclusions : Through the results, it is expected to be used for research and development of new pharmacopuncture and herbal medicine.

생약복합제 GCSB-5의 품질 표준화를 위한 흑두 및 두충의 함량 분석 (Quantitative Analysis of Glycine Semen Nigra and Eucommiae Cortex for Standardization of GCSB-5 Preparation)

  • 이은희;차배천
    • 생약학회지
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    • 제40권1호
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    • pp.18-24
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    • 2009
  • GCSB-5 preparation is a purified extract from a mixture six herbal medicines (Acanthopanacis Cortex, Achyranthis Radix, Saposhnikoviae Radix, Cibotii Rhizoma, Glycine Semen Nigra, Eucommiae Cortex) that have been widely used in traditional medicine to treat various bone disorders. This study was carried out to obtain the HPLC analysis method that can be used to establish quantitative analysis of Glycine Semen Nigra and Eucommiae Cortex for standardization of GCSB-5 preparation. HPLC analysis methods for the simultaneous determination of genistin (Glycine Semen Nigra) and geniposide (Eucommiae Cortex) were established for the quality control of herbal medicinal raw material and preparation. And validation of HPLC analysis methods were conformed for verification of HPLC methods by check to specificity, linearity, intra-day precision, inter-day precision and accuracy following ICH guideline. As the result of quantitative analysis, the contents of genistin and geniposide in the raw material of GCSB-5 preparation were 0.0426-0.0427 mg/g and 0.431-0.432 mg/g. And GCSB-5 preparation contained genistin of 0.0202-0.0203 mg/capsule and geniposide of 0.211-0.212 mg/capsule, respectively.

RAW 264.7 세포에서 레드비트의 항산화 및 항염증 등의 생리활성 연구 (The Study on the Physiological Activities of Beta vulgaris such as Antioxidant and Anti-inflammatory in RAW 264.7 cells)

  • 지중구
    • 한국응용과학기술학회지
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    • 제38권1호
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    • pp.309-317
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    • 2021
  • 본 연구는 침출차 원료로 레드비트를 활용하기 위해서 세포독성, 항염증 및 항산화 등의 활성을 조사하고자 설계되었다. 항산화 효능은 DPPH 및 ABTS 라디컬 소거 활성을 분석하여 평가하였다. RAW 264.7 세포를 통해 MTT 분석으로 세포 생존율을 평가하고 LPS로 유도하여 활성산소종과 염증매개체(일산화질소, TNF-α, IL-6 등)의 생성량을 확인하였다. 그 결과, DPPH 및 ABTS 라디컬 소거활성은 농도 의존적으로 증가하였으며, 모든 농도에서 세포독성이 없음을 확인하였다. 또한, LPS로 유도한 RAW 264.7 세포에서 활성산소종(ROS)과 일산화질소(NO), IL-6, TNF-α 생성량을 유의적으로 감소시켰다. 따라서 이러한 결과는 레드비트가 안전한 항산화 및 항염증 효과를 가진 침출차의 원료로서 높은 잠재력을 가지고 있음을 시사한다.

증숙 온도와 시간에 따른 4년근 인삼의 이화학적 특성 (Physicochemical Characteristics of a 4-Year-Old Ginseng Based on Steaming Temperatures and Times)

  • 유진;장인배;문지원;장인복;이성우;서수정
    • 한국약용작물학회지
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    • 제27권2호
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    • pp.86-95
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    • 2019
  • Background: Depending on the processing method, the raw materials constituents change in various ways. In particular, a heat treatment process, such as steaming, changes the color and aroma of a raw material to increase its palatability and number of physiologically active ingredients. Methods and Results: In the present study, the effects of the steaming temperature and time on the yield, color, proximal composition, and total polyphenol and ginsenoside content of a 4-year-old ginseng root were analyzed. The yield tended to decrease with the increase of steaming time at each temperature and the total ginsenoside content increased with increasing of steaming temperature except at $80^{\circ}C$. Conclusions: These results suggest that steaming at $100^{\circ}C$ for 6 - 9 hours or at $110^{\circ}C$ for 3 - 6 hours is suitable for increasing total polyphenol and ginsenoside content with less yield reduction in a 4-year-old ginseng root.

한약재 중 제랄레논의 탕액으로 이행률 조사 (Investigation of the Transfer Rate of Zearalenone in Herbal Medicines to Their Decoction)

  • 심원보;송정언;김정숙;정영철;정덕화
    • 한국식품과학회지
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    • 제45권3호
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    • pp.312-316
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    • 2013
  • 본 연구에서는 탕액에 존재하는 제랄레논의 분석을 위한 HPLC법을 확립하고 탕액조제 시 한약재로부터의 제랄레논 이행률을 조사하였다. 한약재 (괄루인, 두충, 복분자)에 제랄레논을 임의의 농도로 오염시켜 수침 및 비수침 과정을 거친 후 상압가열 ($100^{\circ}C$, 3 h)과 고압가열 ($121^{\circ}C$, 1 h)하여 탕액을 조제한 다음 탕액과 한약재 잔류물로 분리하고 immunoaffinity column으로 정제하여 분석에 사용하였다. 가열처리를 하지 않은 탕액 제조 전 원료 한약재에 대한 회수율은 괄루인 83.7-95.5%, 두충 81.9-99.7%, 복분자 79.1-82.3%로 분석에 이용이 가능한 것으로 확인되었고, 3종의 한약재 중 회수율이 가장 좋은 두충을 선택하여 탕액을 조제한 후 탕액과 한약재 잔류물에 대한 회수율 측정한 결과 탕액에서는 68.4-83.7%, 한약재 잔류물에서는 72.9-80.3%로 원재료 보다는 낮은 수준으로 확인되었다. 한약재에서 탕액으로 제랄레논의 이행률은 한약재 원재료에 제랄레논을 임의의 농도로 오염시킨 후 탕액을 조제하여 분석하였으며, 그 결과 탕액에서는 제랄레논이 검출되지 않았고, 괄루인과 복분자 잔류물에서는 수침 시료의 경우 17.04-22.99%, 비수침 시료의 경우 10.17-18.33%의 제랄레논이 검출되었다. 그리고 두충 잔류물에서는 수침 시료의 경우 10.44-17.16%, 비수침 시료의 경우 12.42-17.75%가 검출되었다. 본 연구에서는 탕액에서 제랄레논이 검출되지 않아 이행률은 낮은 것으로 판단되나 보다 탕액 조제과정 중 첨가물에 의해 이행이 일어날 수 있는 가능성은 여전히 존재하기 때문에, 제랄레논을 포함한 곰팡이독소에 안전한 한약재의 확보를 위한 지속적인 노력이 필요하다. 또한, 유통 중인 한약재를 포함하여 조제포장되어 판매되는 탕액에 대해서도 지속적인 모니터링이 필요할 것으로 판단된다.

백출에서 분리된 Atractylenolide II의 RAW264.7 대식세포와 BV2 미세아교세포에서의 항염증 효과 (Atractylenoide II Isolated from Atractylodes macrocephala Inhibited Inflammatory Responses in Lipopolysaccharide-induced RAW264.7 Macrophages and BV2 Microglial Cells)

  • 김홍광;김관우;이정;임혜리;이대영;윤다혜;정진태;김금숙;오현철;안인파;김윤철
    • 생약학회지
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    • 제51권4호
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    • pp.244-254
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    • 2020
  • Atractylodes macrocephala is a perennial herb and is a member of the Compositae family. This plant is known to contain various bioactive constituents indicating anti-inflammatory, neuroprotective, anti-oxidant, immunological enhancement, and gastroprotective effects. In this investigation, we isolated four compounds with similar chemical structures from A. macrocephala, and evaluated their anti-inflammatory effects. Among the four compounds, compound 2(atractylenolide II) showed the second-best inhibitory effect on the lipopolysaccharide(LPS)-induced production of nitric oxide in RAW264.7 macrophages and BV2 microglial cells. Compound 2 also inhibited the LPS-induced the production of prostaglandin E2(PGE2), and the expression of inducible nitric oxide synthase(iNOS) and cyclooxygenase(COX)-2 proteins in both cells. In addition, compound 2 suppressed the production of pro-inflammatory cytokines including interleukin(IL)-1β, IL-6, and tumor necrosis factor(TNF)-α. These inhibitory effects were contributed by inactivation of nuclear factor kappa B(NF-κB) and mitogen-activated protein kinases(MAPKs) pathways by treatment with compound 2. This compound did not induce the expression of heme oxygenase(HO)-1 protein indicating that the anti-inflammatory effect of compound 2 was independent with HO-1 protein. Taken together, these results suggested that atractylenolide II can be a candidate material to treat inflammatory diseases.

엉겅퀴 70% 에탄올 추출물의 RAW264.7 세포에서 Heme oxygenase-1 발현을 통한 항염증 효과 (Anti-inflammatory Effect of the Cirsium japonicum var. ussuriense 70% Ethanolic Extract in RAW264.7 Cells by Heme oxygenase-1 Expression)

  • 이동성;김경수;리빈;최현규;케오썸엘;전기용;박준형;김윤철
    • 생약학회지
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    • 제43권1호
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    • pp.39-45
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    • 2012
  • Cirsium japonicum var. ussuriense has long been used in herbal medicine for the treatment of arthritis, dyspepsia, and bleeding in Korea. In the present study, we investigated anti-inflammatory effects of C. japonicum var. ussuriense against lipopolysaccharide(LPS)-induced activation in RAW264.7 cells by the expression of heme oxygenase (HO)-1. The 70% EtOH extract of the aerial parts of C. japonicum var. ussuriense (CJE), showed the potent anti-inflammatory effects on LPS-induced inflammation in RAW264.7 cells. The anti-inflammatory effect of CJE was demonstrated by the suppression of pro-inflammatory mediators, including pro-inflammatory enzymes (inducible nitric oxide synthase and cyclooxygenase-2). Furthermore CJE induced HO-1 expression through nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) and increased HO activity in RAW264.7 macrophages. The effects of CJE on LPS-induced NO and $PGE_2$ productions were partially reversed by an HO-1 inhibitor, tin protoporphyrin (SnPP). Therefore, it is suggested that CJE-induced HO-1 expression plays a role of the resulting anti-inflammatory effects in macrophages. These results suggest that CJE may be a promising candidate for the treatment of inflammatory diseases.

용담화 에탄올 추출물의 항염증 효과에 관한 연구 (Study on Anti-inflammatory Effect of Ethanol Extract of Gentianae sino-ornata)

  • 최형욱;인명희;리순화;김은주;임규상;우원홍
    • 한방안이비인후피부과학회지
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    • 제28권4호
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    • pp.51-61
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    • 2015
  • Objective : This research has been conducted to comparative analysis of anti-inflammatory effects to suggest a usefulness of ethanol extracts fromGentianae sino-ornata(GSO) as a functional material for inflammatory activities.Methods : Cell viability was measured by neutral red (NR) assay, and nitric oxide (NO) production in RAW264.7 cells was monitored by measuring the nitrite content in culture medium. The expressions of cyclooxigenase-2 (COX-2) was determined by western blot analysis, and Inducible nitric oxide syntase (iNOS) and cytokine were determined by reverse transcriptase-polymerase chain reaction (RT-PCR).Results : When the GSO extract was added the concentration of 5-20 ㎍/㎕, the viability of cells was maintained 90% or more at all levels. NO production was suppressed by the treatment of GSO in LPS-stimulated RAW264.7 cells. GSO inhibited the expression of iNOS, COX-2, IL-1βand IL-6 in LPS-stimulated RAW264.7 cells.Conclusions: From this results, we consider that GSO can be a useful therapeutic and preventive approach to various inflammatory diseases as a functional material for inflammatory activities.

계혈등 메탄올추출물이 LPS로 활성화된 Raw264.7 Cell에서 Nitric Oxide 및 Pro-inflammatory Cytokines 생성에 미치는 영향 (Effects of Spatholobi Caulis MeOH Extract on the Production of NO and Pro-inflammatory Cytokines in LPS-activated Raw264.7 Cells)

  • 최송이;박숙자;변성희;이종록;박문기;김상찬
    • 대한본초학회지
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    • 제24권2호
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    • pp.21-27
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    • 2009
  • Objectives: Spatholobi Caulis is the stem of Spatholobus suberectus Dunn., which has been used for treating menstrual disorders, anemia, and rheumatic arthralgia in traditional Oriental medicine, This study was conducted to evaluate the inhibitory effects of Spatholobi Caulis extract (SCE) on the production of nitric oxide (NO) and pro-inflammatory cytokines in LPS-activated Raw264,7 cells. Methods : Cell viability was determined by MIT assay. Relative levels of NO were measured with Griess reagent and pro-inflammatory cytokines were detected by ELISA Expression of iNOS and COX-2 proteins were determined by western blotting. Results : NO production and iNOS expression were increased by incubation with LPS for 24 h. However, the augmented NO was reduced by SCE in a dose-dependent manner. Expressions of iNOS and COX-2 were inhibited by the treatment with SCE, SCE also suppressed the production of pro-inflammatory cytokines, such as IL-1beta and IL-6. Conclusions : These results indicate that the MeOH extract of Spatholobi Caulis warrant further development as an anti-inflammatory agent for the treatment of Gram-negative bacterial infections.

당귀, 작약, 천궁, 삼백초, 인진쑥 및 산조인을 첨가한 한방차의 팽화 온도별 고형분 용출율과 Benzo[${\alpha}$]pyrene의 변화 (Changes in Solid Elution Rate and Benzo[${\alpha}$]pyrene During Puffing Temperature of Herbal Tea Containing Angelica gigas, Paeoniae radix, Cnidium officinale, Saururus chinensis, Artemisia capillarisin and Zizyphus vulgaris)

  • 장재선;최미용;오성천
    • 한국응용과학기술학회지
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    • 제31권2호
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    • pp.305-312
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    • 2014
  • 팽화 처리온도를 각각 $140{\sim}220^{\circ}C$로 한 한방차 제품의 성분변화를 분석한 결과는 다음과 같다. 처리온도의 상승에 따라 일부 탄화가 발생하며 조회분 함량이 상대적으로 상승하는 소폭의 변화가 있었고, 조단백질 및 조지방 함량은 거의 변화가 없는 것으로 나타났으며 수분함량은 감소하였다. 한방차의 고형분 용출률은 0.18~0.27%(w/w)로 나타내었는데, 팽화온도가 상승할수록 증가하였다. 고형분의 용출은 온도가 화학적 변화보다 물리적 변화에 의해 식품의 원재료 성분인 탄수화물, 단백질, 지방 등이 천연 상태에서 상호가교 결합이 물리적인 힘으로 어느 정도 파괴되어 성분의 용출이 용이해지기 때문인 것으로 생각된다. 벤조피렌 함량은 0.18~0.24ppb로 처리온도, 원재료에 따라 B(${\alpha}$)P 함량에 차이가 발생한 것으로 나타났다.