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Anti-inflammatory Effect of the Cirsium japonicum var. ussuriense 70% Ethanolic Extract in RAW264.7 Cells by Heme oxygenase-1 Expression  

Lee, Dong-Sung (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Kim, Kyoung-Su (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Li, Bin (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Choi, Hyun-Gyu (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Keo, Samell (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Jun, Ki-Young (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Park, Jun-Hyeong (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Kim, Youn-Chul (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Publication Information
Korean Journal of Pharmacognosy / v.43, no.1, 2012 , pp. 39-45 More about this Journal
Abstract
Cirsium japonicum var. ussuriense has long been used in herbal medicine for the treatment of arthritis, dyspepsia, and bleeding in Korea. In the present study, we investigated anti-inflammatory effects of C. japonicum var. ussuriense against lipopolysaccharide(LPS)-induced activation in RAW264.7 cells by the expression of heme oxygenase (HO)-1. The 70% EtOH extract of the aerial parts of C. japonicum var. ussuriense (CJE), showed the potent anti-inflammatory effects on LPS-induced inflammation in RAW264.7 cells. The anti-inflammatory effect of CJE was demonstrated by the suppression of pro-inflammatory mediators, including pro-inflammatory enzymes (inducible nitric oxide synthase and cyclooxygenase-2). Furthermore CJE induced HO-1 expression through nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) and increased HO activity in RAW264.7 macrophages. The effects of CJE on LPS-induced NO and $PGE_2$ productions were partially reversed by an HO-1 inhibitor, tin protoporphyrin (SnPP). Therefore, it is suggested that CJE-induced HO-1 expression plays a role of the resulting anti-inflammatory effects in macrophages. These results suggest that CJE may be a promising candidate for the treatment of inflammatory diseases.
Keywords
Cirsium japonicum var. ussuriense; Heme oxygenase-1; RAW264.7; iNOS; COX-2; Inflammation;
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