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http://dx.doi.org/10.6114/jkood.2015.28.4.051

Study on Anti-inflammatory Effect of Ethanol Extract of Gentianae sino-ornata  

Choi, Hyung-Wook (Dept of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang)
In, Myung-Hee (BK21-plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University)
Lee, Shun-Hua (Dept. of Beauty Education, Graduate School of Education, Konkuk University)
Kim, Eun-Joo (Dept of Beauty Health Design, Open Cyber University)
Lim, Kyu-Sang (Dept of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang)
Woo, Won-Hong (Dept. of Anatomy, College of Oriental Medicine, Wonkwang University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.28, no.4, 2015 , pp. 51-61 More about this Journal
Abstract
Objective : This research has been conducted to comparative analysis of anti-inflammatory effects to suggest a usefulness of ethanol extracts fromGentianae sino-ornata(GSO) as a functional material for inflammatory activities.Methods : Cell viability was measured by neutral red (NR) assay, and nitric oxide (NO) production in RAW264.7 cells was monitored by measuring the nitrite content in culture medium. The expressions of cyclooxigenase-2 (COX-2) was determined by western blot analysis, and Inducible nitric oxide syntase (iNOS) and cytokine were determined by reverse transcriptase-polymerase chain reaction (RT-PCR).Results : When the GSO extract was added the concentration of 5-20 ㎍/㎕, the viability of cells was maintained 90% or more at all levels. NO production was suppressed by the treatment of GSO in LPS-stimulated RAW264.7 cells. GSO inhibited the expression of iNOS, COX-2, IL-1βand IL-6 in LPS-stimulated RAW264.7 cells.Conclusions: From this results, we consider that GSO can be a useful therapeutic and preventive approach to various inflammatory diseases as a functional material for inflammatory activities.
Keywords
Gentuanae sino-ornata; LPS; COX-2; iNOS; IL-6;
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