Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Anti-inflammatory Effect of the Cirsium japonicum var. ussuriense 70% Ethanolic Extract in RAW264.7 Cells by Heme oxygenase-1 Expression

엉겅퀴 70% 에탄올 추출물의 RAW264.7 세포에서 Heme oxygenase-1 발현을 통한 항염증 효과

  • Lee, Dong-Sung (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Kim, Kyoung-Su (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Li, Bin (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Choi, Hyun-Gyu (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Keo, Samell (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Jun, Ki-Young (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Park, Jun-Hyeong (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University) ;
  • Kim, Youn-Chul (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
  • Received : 2012.01.27
  • Accepted : 2012.03.09
  • Published : 2012.03.31
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Abstract

Cirsium japonicum var. ussuriense has long been used in herbal medicine for the treatment of arthritis, dyspepsia, and bleeding in Korea. In the present study, we investigated anti-inflammatory effects of C. japonicum var. ussuriense against lipopolysaccharide(LPS)-induced activation in RAW264.7 cells by the expression of heme oxygenase (HO)-1. The 70% EtOH extract of the aerial parts of C. japonicum var. ussuriense (CJE), showed the potent anti-inflammatory effects on LPS-induced inflammation in RAW264.7 cells. The anti-inflammatory effect of CJE was demonstrated by the suppression of pro-inflammatory mediators, including pro-inflammatory enzymes (inducible nitric oxide synthase and cyclooxygenase-2). Furthermore CJE induced HO-1 expression through nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) and increased HO activity in RAW264.7 macrophages. The effects of CJE on LPS-induced NO and $PGE_2$ productions were partially reversed by an HO-1 inhibitor, tin protoporphyrin (SnPP). Therefore, it is suggested that CJE-induced HO-1 expression plays a role of the resulting anti-inflammatory effects in macrophages. These results suggest that CJE may be a promising candidate for the treatment of inflammatory diseases.

Keywords

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