• 한방안이비인후피부과학회지
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• 제29권3호
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• pp.134-149
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• 2016

Objectives : Gal-Geun-Tang (GT) has been described from SANGHAN in Korean traditional medicine and known to act against cold, fever, hypertension, and nasal catarrh. However, little has yet been learned about the effect of GT on immune function. In the current study, in vitro and in vivo immunomodulatory activity of GT (water extract) was investigated.Methods : Water extract of GT induced in vitro proliferation of spleen cells and significantly increased their proliferative responses during anti-CD3 activation. Using purified splenic T and B cells, it was revealed that GT has a mitogenic activity to B cells and promotes their proliferation induced by lipopolysaccharide, whereas T cell proliferation was not triggered and GT was rather inhibitory to T cell activation caused by anti-CD3 antibody. In the presence of antigen presenting cells (APC), GT addition resulted in a significant increase of IFNγ and IL-4, but not IL-2, production. However, addition of high concentration (1,000㎍/㎖) of GT led to a marked reduction in T cell cytokine production and under such condition, GT facilitated apoptosis of T cells when examined by flow cytometry with propidium iodide staining.Results : In vivo immunomdulation of GT was also investigated using a mouse model. Following keyhole limpet hemocyanin (KLH) immunization, GT (1 ㎎/day) was orally administered for 9 days. Cell numbers in thymus, spleen and peripheral blood were not altered by GT administration, indicating that such dose is not immunotoxic. Cell numbers in draining lymph nodes (LN) and ex vivo Ag-specific proliferation of LN cells were significantly elevated by GT administration. However, any preferential stimulation of T or B and CD4⁺ or CD8⁺ T cell subpopulations was not observed in a flow cytometric analysis of LN cells. This result shows that GT does not promote in vivo B cell proliferation while GT enhances Ag-specific proliferation of LN cells, unlike what was observed in vitro.Conclusions : For a further understanding of in vivo immunomodulatory activity of GT, ex vivo cytokine production of LN cells obtained from KLH-immunized mice was evaluated. Ag-specific IFNγ production was significantly higher in GT-treated mice when compared to PBS-treated control mice. In contrast, IL-4 production in GT-treated group was comparable to control group unlike to in vitro data. In addition, GT administration did not result in any significant differences in serum levels of Ig (IgM, IgG1 and IgG2a) between GT-treated and control groups. Taken together, these data strongly support that GT promotes immune response, more profoundly type 1 helper T cell (Th1) activity and GT may be applicable for treatment of intracellular parasite infection such as viral diseases.

• 한국과학교육학회지
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• 제33권4호
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• pp.807-825
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• 2013

이 연구는 과학적 모델의 사회적 구성 과정을 목적으로 설계된 수업을 실행한 두 과학 교사의 과학 수업에서 형성되었던 실천적 지식의 내용을 Elbaz가 제시한 실천적 지식의 5가지 내용 범주를 준거로 조사하였다. 이를 위해 두 과학 교사의 과학 수업에 대한 참여 관찰 및 수업 촬영 비디오 자료를 바탕으로 행위 동안의 반성 과정 및 수업 후 면담에 반영된 교사들의 내러티브를 통한 행위에 대한 반성 과정을 질적으로 분석하였다. 연구 결과, 1) 두 과학 교사의 수업 실행 과정에서 학생들이 과학적인 모델을 구성할 수 있도록 지원하는 비계를 제공할 때 교과내용 지식에 대한 실천적 지식이 발현되는 것을 볼 수 있었다. 2) 교육 과정에 대한 교사의 지식이 어려운 과학 개념의 이해와 전달에서 주어진 학습 목표에 적절한 모델 형성으로 수업의 목표를 변화하는데 중요함을 알 수 있었다. 3) 참여 교사의 교수법 지식의 실행은 과학적 모델의 사회적 구성 수업의 의도에 맞추어 학생들의 모둠 활동과 모델 생성 활동을 지원하는 방향으로 변화하였다. 4) 교사의 자아에 대한 실천적 지식은 학생들이 모둠 활동을 통해 과학적 모델을 구성하는 것을 지원할 수 있으려면, 교사는 조력자, 안내자, 그리고 격려자로서 자신의 역할을 인식하고 변화된 역할을 적절하게 수행하는 것이 중요함을 보여주었다. 5) 두 참여 교사의 학습 환경에 대한 실천적 지식은 수업을 진행하는 동안 각 모둠과 교사가 형성한 상호작용의 양상을 통해 발현되었으며, 두 교사는 함께 뛰는 운동 선수 또는 그라운드 옆의 코치와 같은 양상을 보였다. 이상의 결과를 바탕으로 과학적 모델 구성에 대한 영역 특이적 실천적 지식의 특성에 대해 논의하였다.

• Journal of Ginseng Research
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• 제44권2호
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• pp.282-290
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• 2020

Background: Ginseng is a commonly used herbal medicine in treating various medical conditions. Chronic gut inflammation is a recognized factor for the development of colorectal cancer (CRC). In this project, Asian ginseng berry polysaccharide preparations were used to assess their effects on CRC and related immune regulation mechanisms. Methods: Ginseng berry polysaccharide extract (GBPE) and purified ginseng berry polysaccharide portion (GBPP) were used to evaluate their activities on human HCT-116 and HT-29 CRC cell proliferation. Interleukin-8 secretion analysis was performed on HT-29 cells. Naive CD4 cell isolation and T-helper cell differentiation were performed and determined using flow cytometry for Th1 and Treg in addition to cell cycle and apoptotic investigation. Results: GBPE and GBPP significantly inhibited interleukin-8 secretion and cancer cell proliferation, inhibited CD4⁺IFN-γ⁺ cell (Th1) differentiation, and decreased CD4⁺FoxP3⁺ cell (Treg) differentiation. Compared to the GBPE, GBPP showed more potent antiinflammatory activities on the malignant cells. This is consistent with the observation that GBPP can also inhibit Th1-cell differentiation better, suggesting that it has an important role in antiinflammation, whereas Treg cells hinder the body's immune response against malignancies. Supported by cell cycle and apoptosis data, GBPE and GBPP, at various degrees, remarkably enhanced the anticancer activities of 5-fluorouracil. Conclusion: Data from this project suggested that Asian ginseng berry potentially has clinical utility in managing enteric inflammation and suppressing CRC through immunomodulation mechanisms.

• 한국산학기술학회논문지
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• 제20권1호
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• pp.248-258
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• 2019

본 연구는 유치원에서 자유선택활동을 실시하는 교사의 전문성 향상을 위해 셀프리더십과의 관계를 살펴보고, 교사 효능감에 대한 매개효과를 분석하는 데 목적이 있다. 본 연구는 2018년 9월 17일부터 28일까지 서울 경기 지역 유치원 교사 총 325명에게 방문 조사를 통해 설문을 실시한 자료를 사용하였다. 자료 분석을 위해 SPSS Statistics 22.0과 AMOS 20.0 통계프로그램을 사용하여, 신뢰성분석, 확인적 요인분석, 상관관계 분석, 구조방정식 모형을 실시하였다. 가설검증 결과, 자유선택활동을 교육하는 유치원 교사의 셀프리더십은 교사효능감에 정(+)의 영향을 미치는 것으로 나타났으며, 교사 전문성에도 정(+)의 영향을 미치는 것으로 나타났다. 또한 유치원 교사의 교사효능감도 교사의 전문성에 정(+)의 영향을 미치는 것으로 나타났다. 또한 유치원 교사의 셀프리더십이 교사효능감을 매개하여 교사 전문성에 간접효과가 통계적으로 유의하게 있는 것으로 나타났다. 또한 유치원 교사의 셀프리더십이 교사 전문성에 미치는 직접효과가 유의하게 나타났으며, 이는 유치원 교사의 교사효능감이 셀프리더십과 교사 전문성 간의 관계를 간접 매개함을 확인하였다. 본 연구의 결과를 통하여 유치원 교사의 전문성을 향상시키기 위해서는 교사의 셀프리더십을 강화하는 것은 물론이고, 교사들의 교사효능감 수준을 강화시키기 위해서는 업무환경에 대해 교사들에게 효능감을 높이기 위해 필요한 지식 및 기술 등을 제공하고 교사들의 전문성을 향상시키기 위한 적극적인 촉진자 및 조력자의 역할이 필요할 것이다.

• 한국자원식물학회지
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• 제25권6호
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• pp.682-692
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• 2012

Sea Buckthorn (Hippophae rhamnoides L.) has been used in traditional medicine for the treatment of cough, indigestion, circulatory problems and pain. The associated anti-inflammatory effect of this agent is achieved via the inhibition of Nf-${\kappa}B$ signaling, a property that has been demonstrated to effectively control the symptoms of various skin disorders, including atopic dermatitis. Accordingly, the purpose of this study was to assess the efficacy of Sea Buckthorn in reducing the production of lipopolysaccharide (LPS) activated nitric oxide (NO) by inhibiting the Nf-${\kappa}B$ pathway, as measured by the symptoms of atopic dermatitis (AD) occurring secondarily to inflammation and immune dysregulation. Our data demonstrate that Sea Buckthorn significantly decreased the LPS-induced production of NO (p<0.001). Atopic dermatitis was induced by repeated application of 2,4-dinitrochlorobenzene to the dorsal skin of mice. Topical application of 5% Sea Buckthorn extract improved the symptoms of AD, specifically reducing disease severity scores, scratching behaviors and epidermal thickness. When compared to the control group, animals treated with Sea Buckthorn exhibited increased serum IL-12 levels and decreased serum TNF-${\alpha}$, IL-4 and IL-5 levels. Such a modulation of biphasic T-helper (Th)1/Th2 cytokines may result in a reduction in serum IgE levels. Our findings suggest that mechanism of action of Sea Buckthorn in the treatment of AD is associated with a marked anti-inflammatory effect as well as an inhibition of Th2-mediated IgE overproduction via the modulation of biphasic Th1/Th2 cytokines. Such results suggest that topical Sea Buckthorn extract may prove to be a novel therapy for AD symptoms with few side effects.

• Reproductive and Developmental Biology
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• 제31권1호
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• pp.1-6
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• 2007

NOD/SCID 마우스는 선천성 면역결핍을 지닌 마우스로서 이종 세포 및 조직 이식을 위한 실험동물로서 가장 많이 활용되고 있다. 본 연구는 돼지의 골수조직에서 채취한 조혈줄기세포를 면역결핍마우스의 정맥 주입을 통하여 생체 내 주입을 실시한 결과, 마우스의 조혈조직에서 대단히 높은 돼지 T면역세포의 증식이 관찰되었다. 유세포 분석기를 이용해 돼지 골수 조혈세포 생체 이식 6주의 마우스에서의 돼지 T면역세포의 증식과 분화 특성을 분석한 결과, 마우스 조혈조직인 골수($5.4{\pm}1.9%$), 비장($15.4{\pm}7.3%$), 간($21.3{\pm}1.4%$), 림프절($33.5{\pm}32.8%$)에서 돼지 조혈줄기세포 유래 T 세포의 증식과 분화가 관찰되었고, 돼지 helper T 세포와 cytotoxic T 세포의 발달도 확인되었다. 또한 조직 면역염색을 통하여 마우스의 비장조직에 이식한 돼지 면역세포의 중식을 관찰하였다. 본 연구는 NOD/SCID 마우스를 이용해 돼지 조혈줄기세포로부터 T 면역세포로의 분화 및 발달과정을 생체 내에서 분석할 수 있는 유용한 동물모델로서 이용할 수 있음을 보여준다.

• 보건행정학회지
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• 제7권1호
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• pp.55-72
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• 1997

The purpose of this study is to analyze the consumers' demand pattern and the feature of the market for nursing homes, the number of which is tending upwards. The survey data were obtained from the interview of 500 elderly people living in Seoul and Kyung-Ki provincial area. All respondents were 60 years of age and above. The main findings were summarized as follows: 1. The respondents who are less aged, highly educated comparatively, and living with spouse show positive response for the use of nursing homes. The aged living independently and the aged living with unmarried children show higher demand for this facility. Also, the respondents who prefer independent living away from their childrenn, urban areas as their residence and flat-type housing show more interest for the facility. The respondents who are self- supportive, who has no financial supporter, no caretaker, and no domestic helper demonstrate strong inclination to the use of the facility. The respondents who are interested in this kind of facility, acknowledge the necessity of it show strong intention of moving into it. 2. Logistic regression analysis was conducted to understand factors related to the intention of moving into the nursing homes. The group who wish to live separated from their children in the future give 1.78 times more favorable response than the opposite. The group who have an interest in the facility for elderly has 2.02 times higher intention of moving than the opposite. The group who have an intention of using the facility for elderly it is 7.34 times more likely to move into it. 3. The respondents who are the potential consumers for nursing homes can be subdivided. Within the positive group, it could be divided into the group of living independently with the preference of flat-type housing, the group living independently with the preference of separate housing, and the group wishing to live with their children. Within the negative group, the factor of the division is their concern to the facility. Following this study, it can be said that old age people, who have been regarded as one homogeneous group so far, should be recognized as one characteristic individual. This study also shows that the demand aspect yet in its initial stage shold be researched in anticipation of rapid increase. The understanding of diciding factors, the segmentation of potential market will help work out proper strategy, which will contribute to providers' benefit.

• 한국식품영양과학회지
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• 제45권4호
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• pp.613-618
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• 2016

1,2,3,4,6-Penta-O-galloyl-${\beta}$-D-glucose(PGG)는 오배자(Galla Rhois)의 gallotannin으로 항산화 효과, 항균 효과, 항알레르기 효과 등을 가지는 것으로 알려졌다. 알레르기성질환은 비만세포를 포함한 다양한 면역세포와 관련된 질환이다. 비만세포는 탈과립화에 의한 알레르기 매개성 물질(histamine 및 ${\beta}-hexosaminidase$)의 분비, T helper 2 type 사이토카인(IL-4) 분비 및 염증을 일으키는 매개체($TNF-{\alpha}$)의 증가를 통해 알레르기 반응에서 중요한 역할을 한다. 따라서 본 연구에서는 PGG가 phorbol 12-myristate 13-acetate(PMA)와 A23187로 비만세포인 RBL-2H3 세포에서 탈과립 그리고 Th2 type 및 염증성 사이토카인 생산에 미치는 영향을 조사하고, 관련 메커니즘에 대해 평가하였다. PGG는 PMA와 A23187 자극에 의한 탈과립 마커(histamine 및 ${\beta}-hexosaminidase$)의 분비를 억제하고, IL-4 및 $TNF-{\alpha}$와 같은 사이토카인의 분비를 억제하였다. 이러한 효과는 전사인자인 $NF-{\kappa}B$의 세포질에서 핵으로의 이동을 억제함으로써 나타나는 것으로 판단된다. 이러한 결과로부터 gallotannin의 하나인 PGG가 알레르기 반응을 현저히 저해하는 효과가 있는 것으로 나타나, 향후 알레르기성 질환을 예방, 개선 및 치료하는 데 유용한 물질로 사용될 가능성이 있을 것으로 생각된다.

• 보건행정학회지
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• 제9권1호
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• pp.30-71
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• 1999

There has been a dramatic increase in public awareness regarding dementia during recent years. However, dementia remains a family affair and patients do not receive adequate care in Korea. This study aims to assist patients and their caregivers by establishing Home and Community based Long-Term Care in a city. The data collected for analysis include five main categories: dementia prevalence, limitations of daily activities of patients, burden of caregivers, the services that patient's family want to utilize, the resources that handle dementia in the community. Major findings can be summarized as follows: 1)The prevalence rate of dementia for elderly people is 13.1 per 100 persons. Alzheimer's disease amount to 38.9% of dementia patients and vascular dementia account for 36.7% of them 2)Eight out of ten patients have mild dementia. Almost all patients have normal ADL. IADL, however, shows different picture. In every items of IADL, about 60% of patients reveals some limitations. 3)The proportion of patients who had medical diagnosis is as low as 20%. Families of patients think dementia as normal aging process and medical doctors in the community do not give special concern to dementia patients. 4)Caregivers does not have proper social support. They suffer from long care time, experience large obstacles in respect of health, daily living, and social activity. 5)Health center and Community welfare center have launched some programs-consultation, home-visiting nursing, day care center, voluntary force mobilization and so on-for dementia patients. But they do not perform expected roles and functions because of lack of skilled personnels and inadequate coordination of relevant organizations for dementia care. 6)Families of dementia patients prefer home helper and home-visiting nurse to hospitalization. For the future, however, demand for institution-based long-term services will increase. We develope community dementia care model based on above findings as follows: 1)Health center execute community cardiovascular control program for the prevention of vascular dementia. 2)Refer to epidemiologic characteristics of patients and preference of family, the most urgent task for dementia care in this city is to expand and organize Home and Community based Long-Term Care. 3)For the continuous and comprehensive care, care plan for a patient must be prepared. Case management team should be builded to prepare this plan and coordinate relevant resources. 4)Special long-term care unit for dementia will be needed in a near future. This unit should have multiple functions, such as day-care center, short stay facility, training center for relevant personnels, besides long-term nursing home considering effective care of dementia and efficient operation of the facility. 5)Voluntary workers deserve their due efforts. Incentive mechanisms must be developed to activate voluntary activities.

• 성인간호학회지
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• 제12권1호
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• pp.30-39
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• 2000

Coronary intervention is now a well established method for the treatment of coronary artery disease. Coronary restenosis is one of the major limitations after coronary intervention. So medical teams advise the patients to get the follow-up coronary angiogram in 6 months after coronary intervention to know if the coronary artery stenosis recurs or not. This study was done in order to know how many patients complied with the advice, and to identify the relative factors to the compliance with getting the follow-up coronary angiogram. The subjects were 101 patients (male: 58 female: 22, mean age: $61{\pm}15$), who received coronary interventions from Jan. 1st to Mar. 31st 1997, and their data were collected from them by questionnaires one year after intervention. The questionnaires consisted of family support scale, self efficacy scale and compliance with sick role behavior scale. The result may be summarized as follows. 1. The number of patients who complied with getting the follow-up coronary angiogram were 37 people(36.6%) and did not comply with it were 64 people(63.4%). All scores of family support(t=5.56, p<.0001), self efficacy (t=4.13, p<.0001) and compliance with sick role behavior(t=5.66, p<.0001) were significantly higher in the patients who got the follow-up coronary angiogram than in those who did not get it. But there was not any relative factor in demographic variables (p>.05). 2. The major motivations for getting follow-up coronary angiogram were recurrence of subjective symptom(40.5%), the advice of medical team(32.4%), and fear of recurrence (27.1%). The restenosis rate in patients who got the follow-up coronary angiogram was 37.8%. 3. The restenosis rate was higher in the patients who had subjective symptoms than in those who did not have any subjective symptom. So subjective symptom and restenosis rate showed a high positive correlation(r=39.9, p<.001). However, 27.2% of the patients who did not have any subjective symptom showed coronary restenosis. 4. The reasons why they did not get the follow-up coronary angiogram were economic burden(37.5%), improved symptom(34.4%), busy life schedule(10.9%), fear of invasive procedure(9.4%), negative reaction of family member(3.1%), no helper for patient(3.1%) and worry about medical team's mistake (1.6%). The relative fators on compliance with getting the follow-up coronary angiogram after coronary intervention were family support, self-efficacy and Compliance with sick role behavior. And the most important reason why the patients did not get the follow-up coronary angiogram after coronary intervention was an economic burden.