Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Differentiation and Proliferation of Porcine T Lymphocytes in NOD/SCID Mice

NOD/SCID 모델 마우스 생체 내 돼지 T 면역세포의 증식 및 분화

  • Lee, Yong-Soo (Cell and Gene Therapy Research institute, Graduate School of Life Science and Biotechnology, Pochon CHA University) ;
  • Kim, Tae-Sik (Cell and Gene Therapy Research institute, Graduate School of Life Science and Biotechnology, Pochon CHA University) ;
  • Kim, Jae-Hwan (Cell and Gene Therapy Research institute, Graduate School of Life Science and Biotechnology, Pochon CHA University) ;
  • Chung, Hak-Jae (Animal Biotechnology Division, National livestock Research Institute) ;
  • Park, Jin-Ki (Animal Biotechnology Division, National livestock Research Institute) ;
  • Chang, Won-Kyong (Animal Biotechnology Division, National livestock Research Institute) ;
  • Kim, Dong-Ku (Cell and Gene Therapy Research institute, Graduate School of Life Science and Biotechnology, Pochon CHA University)
  • 이용수 (포천중문의과대학교 세포유전자치료연구소) ;
  • 김태식 (포천중문의과대학교 세포유전자치료연구소) ;
  • 김재환 (포천중문의과대학교 세포유전자치료연구소) ;
  • 정학재 (축산연구소 응용생명공학과) ;
  • 박진기 (축산연구소 응용생명공학과) ;
  • 장원경 (축산연구소 응용생명공학과) ;
  • 김동구 (포천중문의과대학교 세포유전자치료연구소)
  • Published : 2007.03.31
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Abstract

The nonobese diabetic / severe combined immune deficiency (NOD/SCID) has been used for determination of proliferation and differentiation of hematopoietic stem cells as xenotransplantation animal model. In this study, we transplanted porcine hematopoietic cells from bone marrow into NOD/SCID mice via intravenous injection to confirm the activity of differentiation and proliferation for porcine hematopoietic cells in vivo. Interestingly, we observed the result of high efficiency with pig T lymphocytes in hematopoietic organs, liver, spleen lymph node, and bone marrow in NOD/SCID mice. The porcine $CD3^{+}$ T cells were detected with $5.4{\pm}1.9%$ in bone marrow, $15.4{\pm}7.3%$ in spleen, $21.3{\pm}1.4%$ in liver, and $33.5{\pm}32.8%$ in lymph node of NOD/SCID mice at 6 weeks after trans-plantation Furthermore, immunohistochemical analysis showed the high engraftment of porcine T lymphocytes in spleen of NOD/SCID mice. Our data suggest that NOD/SCID mice are excellent animal model to determinate the generation md function of pig T lymphocytes.

NOD/SCID 마우스는 선천성 면역결핍을 지닌 마우스로서 이종 세포 및 조직 이식을 위한 실험동물로서 가장 많이 활용되고 있다. 본 연구는 돼지의 골수조직에서 채취한 조혈줄기세포를 면역결핍마우스의 정맥 주입을 통하여 생체 내 주입을 실시한 결과, 마우스의 조혈조직에서 대단히 높은 돼지 T면역세포의 증식이 관찰되었다. 유세포 분석기를 이용해 돼지 골수 조혈세포 생체 이식 6주의 마우스에서의 돼지 T면역세포의 증식과 분화 특성을 분석한 결과, 마우스 조혈조직인 골수($5.4{\pm}1.9%$), 비장($15.4{\pm}7.3%$), 간($21.3{\pm}1.4%$), 림프절($33.5{\pm}32.8%$)에서 돼지 조혈줄기세포 유래 T 세포의 증식과 분화가 관찰되었고, 돼지 helper T 세포와 cytotoxic T 세포의 발달도 확인되었다. 또한 조직 면역염색을 통하여 마우스의 비장조직에 이식한 돼지 면역세포의 중식을 관찰하였다. 본 연구는 NOD/SCID 마우스를 이용해 돼지 조혈줄기세포로부터 T 면역세포로의 분화 및 발달과정을 생체 내에서 분석할 수 있는 유용한 동물모델로서 이용할 수 있음을 보여준다.

Keywords

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