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Differentiation and Proliferation of Porcine T Lymphocytes in NOD/SCID Mice  

Lee, Yong-Soo (Cell and Gene Therapy Research institute, Graduate School of Life Science and Biotechnology, Pochon CHA University)
Kim, Tae-Sik (Cell and Gene Therapy Research institute, Graduate School of Life Science and Biotechnology, Pochon CHA University)
Kim, Jae-Hwan (Cell and Gene Therapy Research institute, Graduate School of Life Science and Biotechnology, Pochon CHA University)
Chung, Hak-Jae (Animal Biotechnology Division, National livestock Research Institute)
Park, Jin-Ki (Animal Biotechnology Division, National livestock Research Institute)
Chang, Won-Kyong (Animal Biotechnology Division, National livestock Research Institute)
Kim, Dong-Ku (Cell and Gene Therapy Research institute, Graduate School of Life Science and Biotechnology, Pochon CHA University)
Publication Information
Reproductive and Developmental Biology / v.31, no.1, 2007 , pp. 1-6 More about this Journal
Abstract
The nonobese diabetic / severe combined immune deficiency (NOD/SCID) has been used for determination of proliferation and differentiation of hematopoietic stem cells as xenotransplantation animal model. In this study, we transplanted porcine hematopoietic cells from bone marrow into NOD/SCID mice via intravenous injection to confirm the activity of differentiation and proliferation for porcine hematopoietic cells in vivo. Interestingly, we observed the result of high efficiency with pig T lymphocytes in hematopoietic organs, liver, spleen lymph node, and bone marrow in NOD/SCID mice. The porcine $CD3^{+}$ T cells were detected with $5.4{\pm}1.9%$ in bone marrow, $15.4{\pm}7.3%$ in spleen, $21.3{\pm}1.4%$ in liver, and $33.5{\pm}32.8%$ in lymph node of NOD/SCID mice at 6 weeks after trans-plantation Furthermore, immunohistochemical analysis showed the high engraftment of porcine T lymphocytes in spleen of NOD/SCID mice. Our data suggest that NOD/SCID mice are excellent animal model to determinate the generation md function of pig T lymphocytes.
Keywords
Porcine hematopoietic cells; NOD/SCID mouse; Porcine T cells; Engraftment;
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