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http://dx.doi.org/10.1016/j.jgr.2018.12.010

Ginseng berry polysaccharides on inflammation-associated colon cancer: inhibiting T-cell differentiation, promoting apoptosis, and enhancing the effects of 5-fluorouracil  

Wang, Chong-Zhi (Tang Center for Herbal Medicine Research, Department of Anesthesia and Critical Care, University of Chicago)
Hou, Lifei (Program in Cellular and Molecular Medicine, Boston Children's Hospital, Department of Pediatrics, Harvard Medical School)
Wan, Jin-Yi (Tang Center for Herbal Medicine Research, Department of Anesthesia and Critical Care, University of Chicago)
Yao, Haiqiang (Tang Center for Herbal Medicine Research, Department of Anesthesia and Critical Care, University of Chicago)
Yuan, Jinbin (Tang Center for Herbal Medicine Research, Department of Anesthesia and Critical Care, University of Chicago)
Zeng, Jinxiang (Tang Center for Herbal Medicine Research, Department of Anesthesia and Critical Care, University of Chicago)
Park, Chan Woong (Vital Beautie Research Institute, R&D Center, AmorePacific Corporation)
Kim, Su Hwan (Vital Beautie Research Institute, R&D Center, AmorePacific Corporation)
Seo, Dae Bang (Vital Beautie Research Institute, R&D Center, AmorePacific Corporation)
Shin, Kwang-Soon (Department of Food Science and Biotechnology, Kyonggi University)
Zhang, Chun-Feng (Tang Center for Herbal Medicine Research, Department of Anesthesia and Critical Care, University of Chicago)
Chen, Lina (Tang Center for Herbal Medicine Research, Department of Anesthesia and Critical Care, University of Chicago)
Zhang, Qi-Hui (Tang Center for Herbal Medicine Research, Department of Anesthesia and Critical Care, University of Chicago)
Liu, Zhi (Tang Center for Herbal Medicine Research, Department of Anesthesia and Critical Care, University of Chicago)
Sava-Segal, Clara (Tang Center for Herbal Medicine Research, Department of Anesthesia and Critical Care, University of Chicago)
Yuan, Chun-Su (Tang Center for Herbal Medicine Research, Department of Anesthesia and Critical Care, University of Chicago)
Publication Information
Journal of Ginseng Research / v.44, no.2, 2020 , pp. 282-290 More about this Journal
Abstract
Background: Ginseng is a commonly used herbal medicine in treating various medical conditions. Chronic gut inflammation is a recognized factor for the development of colorectal cancer (CRC). In this project, Asian ginseng berry polysaccharide preparations were used to assess their effects on CRC and related immune regulation mechanisms. Methods: Ginseng berry polysaccharide extract (GBPE) and purified ginseng berry polysaccharide portion (GBPP) were used to evaluate their activities on human HCT-116 and HT-29 CRC cell proliferation. Interleukin-8 secretion analysis was performed on HT-29 cells. Naive CD4 cell isolation and T-helper cell differentiation were performed and determined using flow cytometry for Th1 and Treg in addition to cell cycle and apoptotic investigation. Results: GBPE and GBPP significantly inhibited interleukin-8 secretion and cancer cell proliferation, inhibited CD4+IFN-γ+ cell (Th1) differentiation, and decreased CD4+FoxP3+ cell (Treg) differentiation. Compared to the GBPE, GBPP showed more potent antiinflammatory activities on the malignant cells. This is consistent with the observation that GBPP can also inhibit Th1-cell differentiation better, suggesting that it has an important role in antiinflammation, whereas Treg cells hinder the body's immune response against malignancies. Supported by cell cycle and apoptosis data, GBPE and GBPP, at various degrees, remarkably enhanced the anticancer activities of 5-fluorouracil. Conclusion: Data from this project suggested that Asian ginseng berry potentially has clinical utility in managing enteric inflammation and suppressing CRC through immunomodulation mechanisms.
Keywords
Adaptive immune response; Colorectal cancer; 5-Fluorouracil; Ginseng berry polysaccharides; Inflammation;
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