• 한방안이비인후피부과학회지
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• 제22권3호
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• pp.63-70
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• 2009

Objectives : The present study was examined to evaluate the anti-inflammatory effects of the Gentianae Macrophyllae Radix MeOH extracts (GMR) in vivo. Methods : The effects of GMR on anti-inflammation were measured by production of NO, TNF-$\alpha$ (Tumor Necrosis Factor-alpha) and IL-$1{\beta}$ (Interleukin-$1{\beta}$), IL-6 in Raw 264.7 macrophage cells stimulated with LPS. Results : 1. All concentrations of GMR(0.10 mg/ml) had no significant cytotoxicity in Raw 264.7 cell during the entire experimental period. 2. The level of NO and iNOS in culture medium was dramatically increased by LPS application. However, these increases were dose-dependently(0.03 and 0.10 mg/ml) attenuated by treatment with GMR. 3. All concentrations of GMR significantly inhibited the production of IL-$1{\beta}$ in Raw 264.7 macrophage cells stimulated with LPS. Conclusions : These results provide evidences that therapeutic effect of GMR on heat syndrome, especially due to the acute inflammation, are partly due to the reduction of some of inflammatory factors by inhibiting iNOS and COX-2 through the suppression of $p-I{\kappa}B{\alpha}$. Moreover, it suggests that the mechanism of action of GMR comes from the suppression of inflammatory mediators, such as NO, PGE2 and pro-inflammatory cytokines.

• Asian-Australasian Journal of Animal Sciences
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• 제24권1호
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• pp.110-124
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• 2011

Anatomically separate fat depots differ in size, function, and contribution to pathological states such as the metabolic syndrome. We isolated pre-adipocytes from different adipose depots, omental, subcutaneous and intramuscular, of beef cattle, and cultured in vitro to determine the basis for the variations and attribute these variations to the inherent properties of adipocyte progenitors. The proliferating cells from all depots before the confluence were harvested and the proteome was analyzed by a functional proteomic approach, involving 2-DE and MALDI-TOF/TOF. More than 252 protein spots were identified, selected and analyzed by Image Master (ver 7.0) and MALDI-TOF/TOF. Further, our analysis showed that there were specific differences in proteome expression patterns among proliferating precursor cells from the three depots. Sixteen proteins were found to be differentially expressed and these were identified as proteins involved in cellular processes, heat shock/chaperones, redox proteins, cytoskeletal proteins and metabolic enzymes. The results also enabled us to understand the basic roles of these proteins in different inherent properties exhibited by adipose tissue depots.

• Asian Pacific Journal of Cancer Prevention
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• 제17권10호
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• pp.4655-4659
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• 2016

Background and Aim: Breast cancer is a major healthcare problem in women. There are many reports about up-regulation of Hsp27 in cancer tissues but less is known about the potential relationship between Hsp27 antibody levels and breast cancer complications. We here investigated concentrations of serum Hsp27 antigen and antibodies in subjects with and without breast cancer and assessed potential associations with two-year disease-free survival, histological grade and number of lymph nodes. Materials and Methods: Specifically, serum Hsp27 antigen and antibody levels from 97 patients with breast cancer, and 65 healthy controls were determined by enzyme-linkedimmunosorbent assays (ELISAs). Results: Serum Hsp27 and antibody levels were significantly (p<0.001) higher in patients with breast cancer compared to the control group, but no relationship were found with two-year disease free survival, histological grade or number of lymph nodes (p> 0.6, 0.2 and 0.9 respectively). Conclusions: Elevated levels of Hsp27 antibody occur with women with breast cancer but do not appear to be associated with the presence of disease clinical complications.

• 한국동물위생학회지
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• 제41권3호
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• pp.211-216
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• 2018

Heat stroke in a dog is a life-threatening syndrome characterized by a high body temperature over $41^{\circ}C$, by the central nervous system dysfunction, and by multiple organ dysfunction. A 11-month-old male American Pit Bull Terrier was presented to clarify the cause of death to diagnostic laboratory. This dog showed clinical signs such as high body temperature ($42^{\circ}C$), severe tachypnea, hematochezia, epistaxis and hemoptysis after transportation at hot summer time. At necropsy, there were hemorrhages in skin, serosa of stomach and small intestine, and also dark red fluid in lumen of intestine. Histopathologic examination revealed extensive hemorrhages in stomach, muscle, skin, and tongue. In microbiology, pathologic bacteria such as Pasteurella, Boedetella, Salmonella, Clostridium, and circulating virus in Korea such as CDV, CIV, CAV, CHV, CCV, CPIV were negative except CPV. There were many gross and microscopic lesions suggesting hest stroke while pathologic agents and related lesions were not detected. Therefore we diagnosed this case as heatstroke.

• 한국자원식물학회지
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• 제21권1호
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• pp.96-102
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• 2008

Nelumbo nucifera Gaertn.(family Nymphaeaceae) has been used for summer heat syndrome as home remedy in Japan, China and Korea. Although whole plant parts are edible, root is commonly consumed. It has been reported that rhizome extract showed anti-diabetic and anti-inflammatory effects. However, in spite of usefulness for treatment of various diseases, the effect of Nelumbo nucifera rhizome(NNR) on proliferation, migration and matrix degrading enzymes-matrix metalloproteinsases(MMPs), the expression of which degrades extracellular matrix(ECM) leading to metastasis, has not been fully elucidated. We examined the effect of hot water extract of NNR on the proliferation, migration and secretion of MMP-2 and MMP-9 in rat smooth muscle cells(rSMC), epidermoid cancer cells(A431) and breast cancer cells(MDA-MB-231). The proliferation assay was carried out using MTT assay, the principle of which depends upon the conversion of MTT by mitochondrial dehydrogensases of viable cells to formazan crystals. The effect of NNR on migration of cells was examined using wound healing assay. Our results showed that there was inhibition in the proliferation, migration and expression of MMP-2 and MMP-9 in dose dependent fashion in all the cells used. Thus, we concluded that NNR could be used as traditional medicine in the treatment of various diseases where proliferation, migration and MMPs' expression plays a pathological role like in restenosis and metastasis.

• 대한한의학원전학회지
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• 제29권1호
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• pp.1-15
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• 2016

Objectives : The purpose of this study is to investigate the method of setting up logic and estimate Fang Youzhi's work through Shanghanluntiaobian. Methods : Wang Shuhe's old edition Shanghanlun and Fang Youzhi's Shanghanluntiaobian were compared and several essential provisions were analyzed and addressed the positive and negative aspects of Fang Youzhi's revising old edition Shanghanlun mixed bamboo tablets. Results : Fang Youzhi's changing Bianmaifa("辨脈法") and Pingmaifa("平脈法") and placement Bianchishiyemaizhengbingzhi chapter after Liujingbingmaizhengbingzhi for discriminating convulsion dampness summer heat stroke from febrile diseases is appropriate. Fang Youzhi's deletion Shanghanli("傷寒例") chapter that has a guiding characteristics shows his editorial policy. Fang Youzhi's addition Bianwenbingfengwenzabingmaizhengbingzhi chapter is for discriminating febrile disease from epidemic febrile disease wind warmth syndrome miscellaneous disease and it indicates Shanghanlun is a book of treatment after differentiation of syndromes containing epidemic febrile disease and miscellaneous disease. Through Fang Youzhi's revising method I can see several positive aspects. First he tried to refine the logic of the content of Shanghanlun and accord with an actual situation. Second he shows Shanghanlun is a book of treatment after differentiation of syndromes containing epidemic febrile disease and miscellaneous disease and tried to see Shanghanlun clearly. A criticism of the Preserving Old Edition Shanghanlun group could interrupt flexibility and creative thinking reading Shanghanlun and a criticism to the Sangangdingli Principles of the several exegetists is a productive criticism because medicine treats human being so it cannot be completed by a logic. Conclusions : All of these processes are Fang Youzhi's endeavour for strengthening the system and logicality of the old edition Shanghanlun and it is meaningful to show a method to read Shanghanlun to beginners.

• 대한한방내과학회지
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• 제25권3호
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• pp.669-676
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• 2004

Post-herpetic neuralgia(PHN) is a chronic pain syndrome associated with the reactivation of a primary infection with varicella zoster virus(chinken pox), which leads to a chronic infection of dorsal root ganglia. The most common risk factor for shingles and its potential sequela, PHN, is advanced age. For a significant number of patients, the pain following healing of shingles can persist for months to years. If this pain, classified as PHN, persists longer than one month. PHN often leads to depression, disrupted sleep, decreased productivity, and utilization of health care. We treated a 60 year-old female patient who suffered pain and headache after Stellate Ganglion Blocks(SGB). In identifying points for differentiation of syndrom(辨證), this subject was diagnosed as Yangmyeong Merdian wind-heat syndrom(陽明經風熱證) and was administered Seungmagalgeuntanggamibang(revised Shengmagalgen-tang, 升麻葛根湯加味方). To ease pain, Western medication was administered as well. After fourteen days of treatment, pain and other symptoms improved.

• 대한한방내과학회지
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• 제23권1호
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• pp.91-98
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• 2002

Objective : Bangpungtongseongsan(BTS) has been used in oriental medicine for many centuries as a prescription for a heat syndrome of apoplexy. The effects of BTS on the vascular system are not well known. This study is designed to identify the effects of BTS on the regional cerebral blood flow(rCBF), arterial blood pressure and action mechanism in mice. Methods : We measured the change of rCBF and BP by BTS. Secondly, we investigated changes of rCBF and BP for 30 minutes interval after venous inject 0.01, 0.1, 1.0, 10.0mg/kg each density BTS infusion into mice, which were premanaged with methylene blue(10mg/kg, i.v), indomethacin(3mg/kg, i.v.), propranolo(3mg/kg, i.v.) for 30minutes. Results : rCBF increased significantly by BTS in a dose-dependent and BP was not affected by BTS in mice. Pretreatment with methylene blue significantly inhibited rCBF increased by BTS and aceelrated BP not affected by BTS. Pretreatment with indomethacin significantly inhibited rCBF increased by BTS and inhibited BP not affected by BTS. Conclusion : These results show that BTS causes the increasement of rCBF and non-change of BP. The action mechanism is related to prostaglandid activated by cyclooxygenase.

• 대한한방내과학회지
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• 제36권2호
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• pp.122-152
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• 2015

Objectives: This study was designed to develop a standard tool for pattern identification of gastroesophageal reflux disease (GERD) patients. Methods: Korean and Chinese literature was selected that mentioned pattern identification of GERD. We gathered the pattern identification and their symptoms and a Chinese medical doctor proficient in Korean translated the Chinese characters into Korean. A Korean linguist then confirmed the translation results to develop a draft of the standard tool for pattern identification of gastroesophageal reflux disease (PIGERD). The final PIGERD was developed after assessment by an expert committee composed of professors from the Korean Medicine University, using the following items: inclusion of the pattern identification and its symptoms, importance of items, and validity of translation. Results: Six pattern identifications and 94 symptoms were selected from 45 references and translated into Korean. Four pattern identifications [pattern/syndrome of liver qi invading the stomach (肝胃不和), spleen-stomach weakness (脾胃虛弱), spleen-stomach dampness-heat (脾胃濕熱), and stomach yin deficiency (胃陰不足)] and 49 symptoms were then selected through the Delphi method by the expert committee. The final standard PIGERD tool was completed after the assessment of translation validity and reflection of individual opinions by the expert committee. This tool consists of 40 items including tongue and pulse diagnosis. The weighted value was also computed from assessment of the importance of items. Conclusions: We developed a standard tool for pattern identification of gastroesophageal reflux disease (PIGERD) to clarify the pattern identification of patients with gastroesophageal reflux disease for standardized diagnosis.

• 동의신경정신과학회지
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• 제15권1호
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• pp.9-25
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• 2004

Behavioral characteristics of Attention Deficit Hyperactivity Disorder(ADHD) is one of the most common mental disorders among children.child psyachiatry. Inattention, Hyperactivity that is done by hyperkinesis or minimal brain dysfunction is major sypmton in ADHD, But etiology and pathological facor of ADHD is very much or unkown.. We brought to about a Study on the Oriental - medical pathologic Understanding about Inattention, Hyperactivity symptom in ADHD within Don yui bo gam Book are as follows. 1. Oriental medical pathologic concepts about Inattention, Hyperactivity are continuous with process of Yang Qi(陽氣), an unbalance of qi(氣) and shen ming(神明), excess of seven emotions(七情), pathology of Huo(火). 2. Immanent factors in inattention, Hyperactivity are improper diet, overtiredness and seven emotions, are continuous with pathological process of the heart, liver, gall bladder, spleen, stomach, kidneys. 3. In oriental medicine, considered as a child's qi of shao yang, dynamic physiological feature, excess and want of yin and yang, organs and bowels, immanently imbalance in growth rather than a child's mental disorder 4. Inattention, looseness in ADHD-PI type are continuous with forgetfulness, improper overtiredness, shortage of qi, the interior heat syndrome due to yin deficiency within Don yui bo gam Book 5. Hyperactivity, impulsive actions in ADHD-C type are continuous with sudden palpitation, severe palpitation, delirium, fidgeting due to deficiency, fidgetiness, hyperactivity of huo due to yin deficiency, fever, febrile disease with accumulation of blood.