• 제목/요약/키워드: hare

검색결과 126건 처리시간 0.03초

한국멧토끼 ZFX와 ZFY 유전자의 성별 이형성과 분자 성판별 (Molecular Sex Determination Using Sexual Dimorphisms between ZFX and ZFY Genes in Korean Hares(Lepus coreanus Thomas))

  • 한상현;조인철;이성수;오문유;오홍식
    • 생명과학회지
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    • 제17권3호통권83호
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    • pp.402-406
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    • 2007
  • 우리나라에 분포하는 멧토끼 (Lepus coreanus)의 성판별을 위한 분자 표지자를 개발하기 위하여, X, Y 염색체간 상동인 ZFX와 ZFY 유전자들의 성별 이형성에 초점을 맞추어 본 연구를 수행하였다. ZFX와 ZFY 유전자의 인트론 7 영역은 멧토끼의 암수가 구분되는 증폭 양상을 나타내었다. 인트론 7의 길이는 각각 ZFX에서 538, ZFY에서 233-bp로 확인되었다. 특히, ZFX의 인트론 7에서는 RNA-매개성 전위인자 중 한 종이며 토끼의 유전체에서 빈번하게 관찰되는 CSINE2와 유사한 반복서열이 발견되었다. 반면, 반복서열은 ZFY의 인트론 7에서는 관찰되지 않았다. ZFX와 ZFY 유전자의 인트론 7에서 확인된 길이의 차이에 근거하여 중합효소연쇄반응 기법을 이용한 유전자 성판별을 수행하였다. 시험에 이용된 모든 DNA시료들은 ZFX에서 증폭된 공통의 밴드를 가지고 있었다. 이에 반해, 멧토끼 수컷 DNA들은 각각 ZFX와 ZFY에서 증폭된 두 개의 구분되는 밴드들을 나타내었다. ZFX-ZFY 유전자·성판별 결과는 표현형 성별 정보뿐만 아니라 수컷-특이적인 SRY 유전자의 증폭양상과도 일치한 결과와도 정확히 일치하였다. 이상의 결과들은 멧토끼에서 ZFX와 ZFY의 인트론 7 영역간의 성별 이형성은 유전자 성판별을 위한 유용한 유전자 표지자가 될 것으로 사료된다.

개불과 군소육의 단백질 및 아미노산 조성 (Protein and Amino Acid Compositions in Echiurid and Sea Hare Muscles)

  • 최영준;한영실
    • 한국수산과학회지
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    • 제18권6호
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    • pp.550-556
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    • 1985
  • 수산무척추동물에 속하는 개불과 군소의 식품학적 평가를 위하여 육의 단백질 및 아미노산조성을 분석 검토하였으며, in vitro법에 의한 단백질의 품질추정을 위한 실험도 병행하였다. 개불과 군소의 육은 각각 $10.19\%$$6.33\%$의 조단백질을 함유하고 있었다. 이들 각 육은 개불에 있어서는 비단백태질소;$40.6\%$, 수용성단백질 ; $58.9\%$ 염용성단백질 ; $0.2\%$, 알칼리가용성단백질 ; $0.2\%$ 및 기질단백질 ; $0.1\%$, 그리고 군소에 있어서는 비단백태질소 ; $38.8\%$, 수용성단백질 ; $56.9\%$, 염용성단백질 ; $2.5\%$, 알칼리가용성단백질 ; $1.6\%$ 및 기질단백질 ; $0.2\%$로 구성되어 있었다. 양적으로 많은 비율을 점하는 수용성단백질의 subunit 조성을 측정한 결과, 개불은 15개 subunit, 군소는 10개 subunit가 검출되었다. 유리아미노산의 조성을 분석한 결과, 개불은 glycine과 alanine 이 총유리아미노산의 약 $75\%$를 차지 하였고, glycine, alanine, taurine, arginine 및 aspartic acid는 총유리아미노산의 약 $96\%$를 차지하였다. 군소의 총유리아미노산의 양은 개불의 총유리아미노산양의 약 1/10에 불과하였으며 taurine이 총유리아미노산의 약 $78\%$를 차지하였다. 단백질의 아미노산조성을 분석한 결과, 개불의 육단백질에는 glycine, aspartic acid, glutamic acid, arginine, lysine이 많이 함유되어 있었고 cysteine은 그 함량이 적었다. 그리고, 군소의 육단백질에는 glycine, glutamic acid, aspartic acid, arginine이 많이 함유되어 있었으며 cysteine과 tryptophan은 흔적양에 불과하였다. 한편, 개불과 군소육의 가수분해 시료중에는 개불은 glutamic acid, aspartic acid, glycine, arginine, leucine이 그리고 군소는 glycine, glutamic acid, aspartic acid, leucine, serine이 비교적 많은 함량을 보였다. in vitro 법으로 이들 두 동물의 육단백질의 영양가를 추정한 결과, 이미 알려진 수산동물의 육단백질에 비하여 떨어짐을 알 수 있었다.

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Relationship between Nutritionally-related Blood Metabolites and Gastrointestinal Parasites in Nguni Goats of South Africa

  • Gwaze, F. Rumosa;Chimonyo, M.;Dzama, K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제23권9호
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    • pp.1190-1197
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    • 2010
  • The objective of the study was to determine the relationship between faecal egg counts and nutritionally-related blood metabolites in Nguni goats of South Africa. Body weights, body condition scores (BCS), FAMACHA scores, faecal and blood samples were collected from 96 Nguni castrates. Faecal samples were analysed using the modified McMaster technique for nematodes and the sedimentation method for trematodes. Blood was analysed for packed cell volume (PCV), glucose, cholesterol, total protein, albumin, urea and creatinine. Season had an effect on glucose, globulin, total protein, creatinine, PCV and faecal egg counts (FEC). Globulin, PCV, creatinine and FEC were significantly higher in the wet season compared to the dry season. A quadratic relationship existed between faecal egg count loads and BCS whilst negative linear relationships were observed between faecal egg counts and creatinine, albumin and cholesterol levels of Nguni goats.

변분근사식과 연계된 산란체법에 의한 파랑변형 계산 (Computation of Wave Propagation by Scatter Method Associated with Variational Approximation)

  • 서승남
    • 한국해안·해양공학회논문집
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    • 제20권6호
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    • pp.553-563
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    • 2008
  • 만일 임의의 지형을 다수의 계단으로 근사하면 이 지형 위를 지나는 선형 파랑의 변형을 계산하기 위해 변분근사법과 고유함수 전개법을 사용할 수 있다. 본 논문에서는 반사율과 투과율을 계산하기 위해 변분근사식과 연계된 산란체법을 제시하였다. 본 기법은 O'Hare and Davies의 변환행렬 축차법보다 간단하고 직접적인 방법임을 보였다. 또한 수 개의 수치실험을 실시하여 기존 결과와 거의 같은 결과를 얻었다.

Farmers' Perceptions and Knowledge of Cattle Adaptation to Heat Stress and Tick Resistance in the Eastern Cape, South Africa

  • Katiyatiya, C.L.F.;Muchenje, V.;Mushunje, A.
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권11호
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    • pp.1663-1670
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    • 2014
  • The objective of this study was to determine the perceptions and knowledge of farmers of heat stress and tick resistance in cattle. A cross-sectional survey was conducted and 110 farmers in four villages in the sour and sweet velds of the Eastern Cape Province, South Africa were interviewed. The associations among area (municipality), gender, age, level of education, employment and religion were computed using Chi-square tests. The majority of the respondents had on average 4 bulls, 4 cows, 4 heifers, 4 calves, and 4 oxen. Milk was considered as the major (28.3%) reason for keeping cattle. Most farmers owned non-descript (72.6%), and Nguni (45.3%) cattle because of their heat tolerance (54.7%), tick resistance (54.7%), and milking ability (28.2%) traits. Excessive panting (56.6%) and disease transmission (76%) were regarded as the major effects of heat stress and tick infestation in cattle, respectively. About 50% of the respondents agreed that hair length influences tick resistance and 47.17% considered coat colour when acquiring cattle. In the sampled areas, ticks were prevalent in the summer season (93%), and 77.36% of the respondents use acaricides every fortnight. Gall sickness was reported to be a major problem in the cattle herds by 36.79% of the respondents. Our results showed that farmers in the two municipalities had knowledge of cattle adaptation to heat stress and tick resistance.

Bruising in Slaughter Cattle and Its Relationship with Creatine Kinase Levels and Beef Quality as Affected by Animal Related Factors

  • Mpakama, T.;Chulayo, A.Y.;Muchenje, V.
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권5호
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    • pp.717-725
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    • 2014
  • The objective of the study was to determine the effects of animal related factors on bruising in slaughter cattle, creatine kinase (CK) and beef quality. Three hundred and twenty one cattle from three breeds (108 Bonsmara, 130 Beefmaster and 83 Brahman) were used in this study. The animals were grouped as follows: Group 1 (16 months old), Group 2 (18 months old) and Group 3 (24 months old). At exsanguinations, blood samples for CK determination were collected using disposable vacutainer tubes. Muscularis longissimus thoracis et lumborum (LTL) was collected 24 h after slaughter to determine the colour ($L^*$, $a^*$, and $b^*$) and ultimate pH ($pH_u$) of beef. Breed, sex and age had significant effects (p<0.05) on bruising score, CK levels and beef quality. Bonsmara breed had the highest (80%) bruising score percentage, CK ($705.3{\pm}80.57U/L$) and $pH_u$ ($6.3{\pm}0.05$) values while the Bonsmara had the highest $L^*$ ($24.8{\pm}0.78$) $a^*$ ($17.5{\pm}0.53$) and $b^*$ ($12.8{\pm}0.53$) values. Higher CK levels were also observed in winter compared to summer, spring and autumn respectively. Therefore, animal factors (sex, breed and animal age at slaughter) contribute to the development of bruises and have an effect on the levels of CK and meat quality. It was also concluded that there is no significant relationship between meat parameters (L,* $a^*$, and $b^*$) and CK levels.

An outlook on livestock welfare conditions in African communities - A review

  • Njisane, Yonela Zifikile;Mukumbo, Felicitas Esnart;Muchenje, Voster
    • Asian-Australasian Journal of Animal Sciences
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    • 제33권6호
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    • pp.867-878
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    • 2020
  • A significant proportion of the African continent is conducive for animal agricultural production, due to its historical experience and available resources to accommodate and nurture various indigenous and exotic animal species and breeds. With food security being a global challenge, animal products can play an important role as nutrient dense food sources in human diets, particularly in Africa. However, this does not seem to reach its full potential in practice, due to numerous reasons that have not been adequately addressed. Animal welfare reservations can be highlighted as one of the major contributing factors to the curbed progress. The consequences have been scientifically proven to affect product quality and market access. However, in the African community, the concept of animal welfare has not been fully embraced. While there are international animal welfare standards in the developed world, there are inherent factors that hinder adoption of such initiatives in most developing regions, particularly among communal farmers. These include cultural norms and practices, social ranking, socio-economic status, available resources, information dissemination and monitoring tools. Therefore, there is need to harmonize what is internationally required and what is feasible to accommodate global variability. The protocols followed to ensure and evaluate farm animal welfare require regular investigation, innovation and a sustainable approach to enhance animal productivity, efficiency and product quality. Additionally, investing in animal wellbeing and health, as well as empowering communities with significant knowledge, has a potential to improve African livelihoods and contribute to food security. This review seeks to highlight the concept of animal welfare in relation to livestock and food production in African conditions.