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http://dx.doi.org/10.5352/JLS.2007.17.3.402

Molecular Sex Determination Using Sexual Dimorphisms between ZFX and ZFY Genes in Korean Hares(Lepus coreanus Thomas)  

Han, Sang-Hyun (National Institute of Subtropical Agriculture, R.D.A.)
Cho, In-Cheol (National Institute of Subtropical Agriculture, R.D.A.)
Lee, Sung-Soo (National Institute of Subtropical Agriculture, R.D.A.)
Oh, Moon-You (Department of Life Science, College of Natural Sciences, Cheju National Uniersity)
Oh, Hong-Shik (Department of Science Education, College of Education, Cheju National University)
Publication Information
Journal of Life Science / v.17, no.3, 2007 , pp. 402-406 More about this Journal
Abstract
This study was performed to develop the molecular marker for sex determination of hare (Lepus coreanus) distributed in Korea which focused on sexual dimorphism between X and Y chromosomal homologous genes, zinc finger-X (ZFX) and -Y (ZFY). The intron 7 regions of ZFX and ZFY genes exhibited differential amplification patterns between male and female hares. The lengths of intron 7 region of ZFX and ZFY genes were 538 and 233-bp, respectively. Especially, the ZFX intron 7 contained a repetitive sequence identified as member of RNA-mediated transposable elements which was similar to CSINE2 commonly found in the rabbit genome. However, it was not present in intron 7 of ZFY gene. The molecular sex typing by polymerase chain reaction (PCR) was also carried out to determine the sex of hare based on difference in lengths between the intron 7 regions of ZFX and ZFY genes. All DNA samples tested had common band amplified from ZFX. However, the male hare DNAs had two distinct bands which amplified from ZFX and ZFY genes, respectively. The results from ZFX-ZFY PCR sex typing were identical to those from phenotypic investigation and from amplification patterns using male-specific sex determining region Y (SRY) gene as well. Finally, this study suggested that the sexual dimorphism between intron 7 regions of ZFX and ZFY could be useful genetic marker to determine sex of hare.
Keywords
sexual dimorphism; ZFX; ZFY; hare; SINE;
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1 Thompson,J. D., D. G. Higgins and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting position specific gap penalties and weight matrix choice. Nucl. Acids Res. 22, 4673-4680   DOI   ScienceOn
2 Tomomasa, H., Y. Adachi, M. Iwabuchi, Y. Tohyama, M. Yotsukura, S. Oshio, T. Yazaki, T. Umeda, T. Takano, Y. Yamanouchi and Y. Nakahori. 1999. XX-male syndrome bearing the sex-determining region Y. Arch. Androl. 42, 89-96   DOI
3 Zenteno, J. C., S. Carranza-Lira, A. L. Jimenez and S. Kofrnan, 2003. A de novo phe67leu mutation in the SRY gene in a patient with complete 46,XY gonadal dysgenesis. J. Endocrinol. Invest. 26, 1117-1119   DOI   ScienceOn
4 McCarty, A. S., G. Kleiger, D. Eisenberg and S. T. Smale. 2003. Selective dimerization of a C2H2 zinc finger subfamily. Mol. Cell 11, 459-470   DOI   ScienceOn
5 Medstrand, P., L. N. van de Lagemaat and D. L. Mager. 2002. Retroelement distributions in the human genome: variations associated with age and proximity to genes. Genome Res. 12, 1483-1495   DOI   ScienceOn
6 Melniczek, J. R., D. Dambach, U. Prociuk, P. F. Jezyk, P. S. Henthorn, D. F. Patterson and U. Giger. 1999. Sry-negative XX sex reversal in a family of Norwegian Elkhounds. J. Vet. Intern. Med. 13, 564-569   DOI
7 Nagai, K. 2001. Molecular evolution of Sry and Sox gene. Gene 270, 161-169   DOI   ScienceOn
8 Poloumienko, A. 2004. Cloning and comparative analysis of the bovine, porcine, and equine sex chromosome genes ZFX and ZFY. Genome 47, 74-83   DOI   ScienceOn
9 Pomp, D., B. A. Good, R. D. Geisert, C. J. Corbin and A. J. Conley. 1995. Sex identification in mammals with polymerase chain reaction and its use to examine sex effects on diameter of day-10 or -11 pig embryos. J. Anim. Sci. 73, 1408-1415   DOI
10 Sinclair, A. H., P. Berta, M. S. Palmer, J. R. Hawkins, B. L. Griffiths, M. J. Smith, J. W. Foster, A. -M. Frischauf, R. Lovell-Badge and P. N. Goodfellow. 1990. A gene from the human sex-determining region Y encodes a protein with a homology to a conserved DNA-binding motif. Nature 346, 240-244   DOI   ScienceOn
11 Smit, A. F. 1996. The origin of interspersed repeats in the human genome. Curr. Opin. Genet. Dev. 9, 657-663   DOI   ScienceOn
12 Geraldes, A., C. Rogel-Gaillard and N. Ferrand. 2005. High levels of nucleotide diversity in the European rabbit (Oryctolagus cuniculus) SRY gene. Anim. Genet. 36, 349-351   DOI   ScienceOn
13 Staessen, C., E. van Assche, H. Joris, M. Bonduelle, M. Vandervorst, I. Liebaers and A. van Steirteghem. 1999. Clinical experience of sex determination by fluorescent in-situ hybridization for preimplantation genetic diagnosis. Mol. Hum. Reprod. 5, 382-389   DOI   ScienceOn
14 Gardner, R. L. and R. G. Edwards. 1968. Control of the sex ratio at full term in rabbit by transferring sexed blastocysts. Nature 218, 346-349   DOI   ScienceOn
15 Gentles, A. J., O. Kohany and J. Jurka. 2005. Evolutionary diversity and potential recombinogenic role of integration targets of Non-LTR retrotransposons. Mol. Biol. Evol. 22, 1983-1991   DOI   ScienceOn
16 Geraldes, A. and N. Ferrand. 2006. A 7-bp insertion in the 3' untranslated region suggests the duplication and concerted evolution of the rabbit SRY gene. Genet. Sel. Evol. 38, 313-320   DOI   ScienceOn
17 Horng, Y. M. and M. C. Huang. 2003. Male-specific DNA sequences in pigs. Theriogenology 59, 841-848   DOI   ScienceOn
18 Jurka. J. 2006. CSINE2: A large family of SINE elements from rabbit. Repbase Reports 6, 208
19 Kim Y. K. 1994. A comparison of nutrient digestibility by wild Korean mountain hares (Lepus sinensis coreanus) and rabbits (Oryctolagus cuniculus). Korean J. Acnim. Sci. Technol. 36, 397-402
20 King, W. A., T. Linares, I. Gustavesson and A. A. Bane. 1979. A method for preparation of chromosome from bovine zygotes and blastocytes. Vet. Sci. Commun. 3, 51-56   DOI
21 Barr, M. L. 1960. Sexual dimorphism interphase nuclei. Am. J. Hum. Genet. 12, 118-127
22 Koh, H. S., T. Y. Chun, H. S. Yoo, Y. Zhang, J. Wang, M. Zhang and C. Wu. 2001. Mitochondrial cytochrome b gene sequence diversity in the Korean hare, Lepus coreanus Thomas (Mammalia, Lagomorpha). Biochem. Genet. 39, 417-429   DOI   ScienceOn
23 Lander, E. S., L. M. Linton and B. Birren. 2001. Initial sequencing and analysis of the human genome. Nature 409, 860-921   DOI   ScienceOn
24 Anderson, G. B. 1987. Identification of embryonic sex detection of H-Y antigen. Theriogenology 27, 81-97   DOI   ScienceOn
25 Quilter, C. R., S. C. Blott, A. J. Mileham, N. A. Affara, C. A. Sargent and D. K. Griffin, 2002. A mapping and evolutionary study of porcine sex chromosome genes. Mamm. Genome 13, 588-594   DOI   ScienceOn
26 Bello, N. and A. Sanchez. 1999. The identification of a sex-specific DNA marker in the ostrich using a random amplified polymorphic DNA (RAPD) assay. Mol. Ecol. 8, 667-669   DOI   ScienceOn
27 Birren, B., E. D. Green, S. Klapholz, R. M. Myers and J. Roskams. 1997. Genome analysis: A laboratory manual. Cold Spring Harbor Laboratory Press, USA
28 Cho, I. C., S. Y. Kang, S. S. Lee, Y. L. Choi, M. S. Ko, M. Y. Oh and S. H. Han. 2005. Molecular sexing using SRY and ZF genes in pigs. Korean J. Anim. Sci. Technol. 47, 317-324   DOI   ScienceOn