• Title/Summary/Keyword: half-cell method

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Nitrate uptake of the red tide dinoflagellate Prorocentrum micans measured using a nutrient repletion method: effect of light intensity

  • Lee, Kyung Ha;Jeong, Hae Jin;Kim, Hye Jeong;Lim, An Suk
    • ALGAE
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    • v.32 no.2
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    • pp.139-153
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    • 2017
  • The ability of a red tide species to take up nutrients is a critical factor affecting its red tide dynamics and species competition. Nutrient uptake by red tide species has been conventionally measured by incubating nutrient-depleted cells for a short period at 1 or 2 light intensities. This method may be applicable to certain conditions under which cells remain in oligotrophic water for a long time and high nutrients are suddenly introduced. Thus, a new method should be developed that can be applicable to the conditions under which cells are maintained in eutrophicated waters in healthy conditions and experience light and dark cycles and different light intensities during vertical migration. In this study, a new repletion method reflecting these conditions was developed. The nitrate uptake rates of the red tide dinoflagellate Prorocentrum micans originally maintained in nitrate repletion and depletion conditions as a function of nitrate concentration were measured. With increasing light intensity from 10 to $100{\mu}E\;m^{-2}s^{-1}$, the maximum nitrate uptake rate ($V_{max}$) of P. micans increased from 3.6 to $10.8 pM\;cell^{-1}d^{-1}$ and the half saturation constant ($K_{s-NO3}$) increased from 4.1 to $6.9{\mu}M$. At $20{\mu}E\;m^{-2}s^{-1}$, the $V_{max}$ and $K_{s-NO3}$ of P. micans originally maintained in a nitrate repletion condition were similar to those maintained in a nitrate depletion condition. Thus, differences in cells under nutrient repletion and depletion conditions may not affect $K_{s-NO3}$ and $V_{max}$. Moreover, different light intensities may cause differences in the nitrate uptake of migratory phototrophic dinoflagellates.

In Vivo Development of Vitrified Rat Embryos: Effects of Timing and Sites of Transfer to Recipient Females

  • Han, Myung-Sook;Koji Niwa;Magosaburo Kasai
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.80-80
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    • 2003
  • In cryopreserved rat embryos, survival rates obtained in vitro are not always consistent with the rates obtained in vivo. To determine the optimal conditions for in vivo development to term, rat embryos at the 4-cell, 8-cell and morula stages were vitrified in EFS40 by a 1-step method and transferred into oviducts or uterine horns of recipients at various times during pseudopregnancy. Vitrified and fresh 4-cell embryos only developed after transfer into oviducts of asynchronous recipients on Day -1 to -2 of synchrony, i.e., at a point in pseudopregnancy that was 1-2 days earlier than the embryos. However, although about half the vitrified embryos transferred into oviducts on Day -1 developed to term, only a minority of embryos transferred at later times did so, whether vitrified (10-34%) or fresh (24-33%), suggesting that this may not be the most suitable stage for cryopreservation. Very few 8-cell embryos, either vitrified or fresh, developed when transferred into oviducts on Day 0 to -0.5. However, when transferred into uterine horns, high proportions of vitrified 8-cell embryos (-63%) developed to term in reasonably synchronous recipients (Day 0 to -0.5) but not in more asynchronous ones (6%; Day-1). A majority of vitrified morulae also developed to term (52-68%) in a wider range of recipients (Day 0 to -1), the greatest success occurring with recipients on Day -0.5. Similar proportions of vitrified and fresh 4-cell embryos, 8-cell embryos and morulae developed to term when there was appropriate synchronization between embryo and recipient. Thus vitrification of preimplantation stage rat embryos does not appear to impair their developmental potential in vivo.

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An Efficient Hardware Implementation of AES Rijndael Block Cipher Algorithm (AES Rijndael 블록 암호 알고리듬의 효율적인 하드웨어 구현)

  • 안하기;신경욱
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.12 no.2
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    • pp.53-64
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    • 2002
  • This paper describes a design of cryptographic processor that implements the AES (Advanced Encryption Standard) block cipher algorithm, "Rijndael". An iterative looping architecture using a single round block is adopted to minimize the hardware required. To achieve high throughput rate, a sub-pipeline stage is added by dividing the round function into two blocks, resulting that the second half of current round function and the first half of next round function are being simultaneously operated. The round block is implemented using 32-bit data path, so each sub-pipeline stage is executed for four clock cycles. The S-box, which is the dominant element of the round block in terms of required hardware resources, is designed using arithmetic circuit computing multiplicative inverse in GF($2^8$) rather than look-up table method, so that encryption and decryption can share the S-boxes. The round keys are generated by on-the-fly key scheduler. The crypto-processor designed in Verilog-HDL and synthesized using 0.25-$\mu\textrm{m}$ CMOS cell library consists of about 23,000 gates. Simulation results show that the critical path delay is about 8-ns and it can operate up to 120-MHz clock Sequency at 2.5-V supply. The designed core was verified using Xilinx FPGA board and test system.

The Effect of Corrosion Inhibitors Influencing on the Corrosion of Reinforced Steel in Mortar Specimen Immersed in Sea Water for Seven Years (해수중 7년간 침지된 몰탈시험편의 철근부식에 미치는 부식억제제의 효과)

  • 정진아;남진각;문경만;이명훈;김기준
    • Proceedings of the Korea Concrete Institute Conference
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    • 1998.10c
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    • pp.211-216
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    • 1998
  • Recently the shortage of good aggregate has encouraged the use of sea sand in construction field, and the corrosion damage of the reinforced steel in concrete structures has been increased due to chlorides from sea sand and deicing salt. Therefore, a number of researchs are proceeding to prevent the corrosion of the reinforced steel in concrete, especially in marine environments. This study focused on the effect of corrosion inhibitors to evaluate protection characteristics for mortar specimens containing clorides. Corrosion behaviors have been investigated by half-cell potential measurement, linear polarization method, AC impedance method, and cyclic polarization test after immersing in sea water for 7 years. A possitive effect of a corrosion inhibitor has been obtained.

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Growth and Characterization of SrS:Ce Thin Films for Blue EL Devices (청색발광 EL소자용 SrS:Ce박막의 제작과 기초적 물성)

  • 이상태
    • Journal of Advanced Marine Engineering and Technology
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    • v.25 no.6
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    • pp.1272-1280
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    • 2001
  • SrS:Ce thin films for blue EL devices were prepared by Hot Wall Method and their crystallographic and optical characteristics were investigated by various methods. Deposition rates were increased with SrS cell temperature, but the rates were independent on substrate temperature and sulfur pressure. The optical and crystallographic characteristics were strongly affected by deposition rates. The band gap energies obtained by optical transmission spectra and Full Width at Half Maximum of (200) plane in X-ray diffraction patterns were found at 4.5-4.6eV and $0.22~0.26^{\circ}$, respectively. The photoluminescence from SrS:Ce thin tiles showed a greenish blue omission peaked at 470 and 540nm.

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Growth and characterization of CdTe single crystal by vertical Bridgman method (수직 Bridgman 법에 의한 CdTe 단결정 성장과 특성)

  • Hong, Myung-Seok;Hong, Kwang-Joon
    • Journal of Sensor Science and Technology
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    • v.14 no.6
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    • pp.369-373
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    • 2005
  • High quality CdTe single crystal for the solar cell fabrication was grown by vertical Bridgman method. The etch pits patterns of (111) surfaces of CdTe etched by Nakagawa solution was observed the (111)A compesed of Cd atoms with typical triangle etch pits of pyramid mode. From the photoluminescence measurement on (111)A, we observed free exciton ($E_{x}$) existing only high quality crystal and neutal acceptor bound exciton ($A^{0}$,X) having very strong peak intensity. Then, the full width at half maximum and binding energy of neutral acceptor bound exciton were 7 meV and 5.9 meV, respectively. By Haynes rule, an activation enery of impurity was 59 meV. Therefore, the origins on impurity level acting as a neutral acceptor were associated Ag or Cu elements.

Photoluminescent properties for CdTe crystal grown by Bridgman method (Bridgman 방법으로 성장된 CdTe의 광발광 특성)

  • Hong, Kwang-Joon;You, Sang-Ha
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2004.04b
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    • pp.42-45
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    • 2004
  • High quality CdTe single crystal for the solar cell fabrication was grown by vertical Bridgman method. The etch pits patterns of {111}surfaces of CdTe etched by Nakagawa solution was observed the {111} A composed of Cd atoms with typical triangle etch pits of pyramid mode. From the photoluminescence measurement on {111}A, we observed free exciton($E_x$) existing only higy quality crystal and neytral acceptor bound exciton($A^0$,X) having very strong peak intensity. Then, the full width at half maximum and binding energy of neutral acceptor bound exciton were 7 meV and 5.9 meV, respectively. By Haynes rule, an actibation energy of impurity was 59 meV. Therefore, the origins on impurity level acting as a neutral acceptor were associated Ag or Cu elements.

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The Electrochemical Study of the Concrete Reinforcement Corrosion (콘크리트내부의 철근부식에 관한 전기화학적연구)

  • 강태혁;조원일;신치범;김은겸;주재백;윤경석
    • Proceedings of the Korea Concrete Institute Conference
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    • 1996.10a
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    • pp.213-217
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    • 1996
  • The electrochemical methods of early detection and analysis of corrosion related deterioration in concrete reinforcement structures are very useful techniques. The generally using procedure for corrosion monitoring of reinforced structures employs a method of half-cell potential measurement. Whilst the technique has provided a useful means of delineating areas of high or low corrosion risk, there are difficulties in its use and interpretation, particularly when assessing corrosion rates of reinforcement. The aim of this study is to describe the AC-impedance method being employed to monitor and assess corrosion rates, to estimate corrosion mechanism of reinfrocement in laboratory conditions. The AC-impedance monitoring technique applies a small amplitude(20mV) AC signal to embedded steel in concrete and reference electrode (Cu/$CuSo_4$). We obtained over a wide frequency range(10MHz~1mHz) to produce a complex plane plot or Nyquist plot.

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Estimation of Critical Chloride Threshold Value Using Corrosion Monitoring (부식 모니터링을 이용한 콘크리트 내의 임계 염화물량 평가)

  • Bae Su Ho;Lee Kwang Myong;Chung Young Soo;Kim Jee Sang
    • Proceedings of the Korea Concrete Institute Conference
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    • 2004.11a
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    • pp.801-804
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    • 2004
  • It should be noted that the critical chloride threshold level is not considered to be a unique value for all conditions. This value is dependent on concrete mixture proportions, cement type and constituents, presence of admixtures, environmental factors, steel reinforcement surface conditions, and other factors. In this study, the accelerated corrosion test for reinforcing steel was conducted by electrochemical and sea water-circulated method, respectively and during the test, corrosion monitoring by half cell potential method was carried out to estimate the critical chloride threshold value when corrosion for reinforcing steel in concrete was perceived. For this purpose, lollypop and beam test specimens were made for $31.4\%,\;41.5\%\;and\;49.7\%$ of w/c. respectively and then the accelerated corrosion test for reinforcing steel was executed. It was observed from the test that the time to initiation of corrosion was found to be different with water-cement ratio and the critical chloride threshold value was found to range from 0.91 to $1.27kg/m^3$.

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An Experimental Study on Measurement of Corrosion Initiation in Reinforced Concrete Exposed to Chloride Using EIS Method (EIS를 이용한 염해에 노출된 철근콘크리트의 부식개시 측정에 관한 실험적 연구)

  • Park, Dong-Jin;Park, Jang-Hyun;Lee, Kwang-Soo;Lee, Han-Seung
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2017.11a
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    • pp.61-62
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    • 2017
  • In this study, the initiation of steel corrosion was monitored due to chloride attack using embedded sensor. In general, Steel bars embedded in concrete are protected from corrosion by being forming a passive film on the surface. However, the passive film is destroyed by chemical erosion such as concrete carbonation and chloride penetration, and the rebar is exposed to the deteriorating factor and corrosion proceeds. In order to realize the initiation of steel corrosion, OCP and change of Impedance parameter were observed by using Half-cell and EIS method depending on cover depth. As result, 10mm cover showed the impedence increased in 6weeks.

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