• 대한전기학회:학술대회논문집
• /
• 대한전기학회 1994년도 하계학술대회 논문집 C
• /
• pp.1571-1573
• /
• 1994

In order to substitute for the conventional measuring system which could bring about technical inconveiences, measuring techniques for the fast transient high voltage upto 100 kV and small signals less than 1 V are developed by use of Laser Source with Packets cell. for the former, capacitive voltage divider was specially designed for reducing the impulse voltage less than the half-wave voltage of pockets cell. For the tatter, interferometer type was employed as a mean to removing the fluctuation of Laser output intensity. And also the main beam through the Pockels cell and the reference beam from the Laser source are seperated before being detected respectively by photo diodes. And then, these two signals are amplified and compared for detecting only the small signals applied across the Pockels cell. Throughout this work, Laser-based measuring system is likely to enable us, at this moment, to detect correctly lightning impulse voltage upto 100 kV and the small signals less than 1 V upto the 2 MHz. Such a system could be employed as a possible diagnostic measuring system at the substation.

• Journal of Microbiology and Biotechnology
• /
• 제22권10호
• /
• pp.1395-1400
• /
• 2012

In this study, we investigated various cultural and operational factors to enhance electricity generation in a microbial fuel cell (MFC) using Geobacter sulfurreducens. The pure culture of G. sulfurreducens was cultivated using various substrates including acetate, malate, succinate, and butyrate, with fumarate as an electron acceptor. Cell growth was observed only in acetate-fed medium, when the cell concentrations increased 4-fold for 3 days. A high acetate concentration suppressed electricity generation. As the acetate concentration was increased from 5 to 20 mM, the power density dropped from 16 to $13mW/m^2$, whereas the coulombic efficiency (CE) declined by about half. The immobilization of G. sulfurreducens on the anode considerably reduced the enrichment period from 15 to 7 days. Using argon gas to create an anaerobic condition in the anode chamber led to increased pH, and electricity generation subsequently dropped. When the plain carbon paper cathode was replaced by Pt-coated carbon paper (0.5 mg $Pt/cm^2$), the CE increased greatly from 39% to 83%.

• 대한의생명과학회지
• /
• 제27권3호
• /
• pp.134-141
• /
• 2021

c-Jun N-terminal kinases (JNKs) have a Janus face, regulating both cell apoptosis and survival. The present study focused on understanding the function of JNK in tumor development and the chemoresistance underlying JNK-mediated cancer cell survival. We identified an inhibitor of JNK1, an important regulator of cancer cell survival. Kinase assay data showed that JNK1-dependent c-Jun phosphorylation was inhibited by indirubin derivatives. In particular, indirubin-3-monoxime (I3M) directly inhibited the phosphorylation of c-Jun in vitro, with a half inhibition dose (IC₅₀) of 10 nM. I3M had a significant inhibitory effect on JNK1 activity. Furthermore, we carried out assays to determine the viability, migration, and proliferation of breast cancer cells. Our results demonstrated that cell growth, scratched wound healing, and colony forming abilities were inhibited by the JNK inhibitor SP600125 and I3M. The combination of SP600125 and I3M significantly decreased cancer cell proliferation, compared with either SP600125 or I3M alone. Our studies may provide further support for JNK1-targeting cancer therapy using the indirubin derivative I3M in breast cancer.

• Journal of Applied Biological Chemistry
• /
• 제63권4호
• /
• pp.457-462
• /
• 2020

Pancreatic cancer is among the most difficult-to-treat tumors. More than half of patients with this cancer have very few symptoms at the early stages, allowing the development of distant metastases and resistance to cancer treatment. In this study, we found that Juniperus chinensis extract (JCX) decreased the cell viability and migration activity of PANC-1 and SNU-213 pancreatic cancer cells in a dose-dependent manner. JCX increased caspase-3 activation and generation of reactive oxygen species (ROS). N-acetylcysteine treatment blocked JCX-induced ROS generation and the negative effects on pancreatic cancer cell viability. In addition, JCX down-regulated the levels of phospho-focal adhesion kinase (p-FAK) and phospho-extracellular signal-regulated kinase (p-ERK). Together, these results indicate that JCX induces apoptosis in human pancreatic cancer cell lines through ROS production, downregulating FAK/ERK signaling and activating caspase-3. We propose that JCX-derived compounds represent candidates for the development of alternative medicines for the treatment of pancreatic cancer.

• Current Photovoltaic Research
• /
• 제11권1호
• /
• pp.34-37
• /
• 2023

Solar energy had become the main energy industry of renewable energy along with hydroelectric power generation. One of the technologies that contributed to the popularization of photovoltaic power and the decrease in the unit price of photovoltaic modules was the large-area solar cell. However, as the area increased, the light receiving area increased and the current value increased accordingly. Since power loss occurs when the current value was large, the number of busbar was increased to increase the current collection rate, and a technology to lower the current value through half-cutting was developed. The bus bar of the solar cell served as a passage through which the generated current was transmitted. This was because when the number of busbar decreases, the moving distance of electrons increased, so the amount of power generation decreases and when it increases, shadows occured. An important aspect of the electrode design was the optimal balance of these busbars and number of fingers. Therefore, in this study, the characteristics of the solar cell according to the number of front bus bars of the large-area solar cell were simulated using Griddler 2,5 pro. After selecting the number of busbar with the best characteristics, the difference was compared by varying the number of fingers and a better direction for the number of cutting was presented.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제7권5호
• /
• pp.303-310
• /
• 2002

Cell therapy applied to wound healing or tissue regeneration presents a revolutionary realm to which principles of gene engineering and delivery may be applied. One promising application is the transplantation of cells into the wounded tissue to help the tissue repair. However, when cells are transplanted from in vitro to in vivo, immune rejection occurs due to the immune response triggered by the activation of T-cell, and the transplanted cells are destroyed by the attack of activated T-cell and lose their function. Immune suppressant such as FK506 is commonly used to suppress immune rejection during transplantation. However, such kind of immune suppressants not only suppresses immune rejection in the periphery of transplanted cells but also suppresses whole immune response system against pathogenic infection. In order to solve this problem, we developed a method to protect the desired cells from immune rejection without impairing whole immune system during cell transplantation. Previously, we reported the success of constructing glomerular epithelial cells for removal of immune complex, in which complement receptor of type 1 (CR1) was over-expressed on the membrane of renal glomerular epithelial cells and could bind immune complex of DNA/anti-DNA-antibody to remove immune complex through phagocy-tosis [1]. Attempting to apply the CR1-expressing cells to cell therapy and evade immune rejection during cell transplantation, we constructed three plasmids containing genes encoding a soluble fusion protein of cytolytic T lymphocyte associated antigen-4 (CTLA4Ig) and an enhanced green fluorescent protein (EGFP). The plasmids were transfected to the above-mentioned glomerular epithelial cells to express both genes simultaneously. Using the clone cells for cell transplantation showed that mice with autoimmune disease prolonged their life significantly as compared with the control mice, and two injections of the cells at the beginning of two weeks resulted in remarkable survivability, whereas it requires half a year and 50 administrations of proteins purified from the same amount of cells to achieve the same effect.

• 전기화학회지
• /
• 제3권1호
• /
• pp.44-48
• /
• 2000

상온에서 DC-magnetron sputtering으로 증착한 비정질의 $V_2O_5$ 박막을 양극물질로 하여 $V_2O_5/LIPON/Li$으로 구성된 박막형 리튬이차전지를 제작하였다. $V_2O_5$의 양극특성은 액체전해질을 이용한 half cell 구조에서 평가하였으며, $Ar/O_2$ 분압비의 변화에 따라 제작된 $V_2O_5$ 양극은 분압비 80/20에서 가장 좋은 특성을 보였다. 자체 제작한 $Li_3PO_4$ 타겟을 사용하여 RF-sputtering으로 순수한 질소 분위기 하에서 양극 위에 고체전해질 LIPON 박막을 형성하였으며, 1.2-4.0V vs. Li 구간에서 리튬에 대해 반응성이 없는 안정한 화합물임을 확인하였다. 음극으로 쓰인 약 $2{\mu}m$두께의 금속리튬박막은 진공 열 증착법으로 제조하였으며, $V_2O_5/LIPON/Li$의 박막형 리튬이차전지는 $1.2\~3.5V$ 구간에서 초기에 약 $150{\mu}A/cm^2{\mu}m$의 높은 방전용량을 나타내었다.

• 한국수소및신에너지학회논문집
• /
• 제16권1호
• /
• pp.31-39
• /
• 2005

Direct methanol fuel cell (DMFC) is considered as a candidate for portable power sources, that could overcome the disadvantages of lithium battery. But in order to attain commercial viability the long term stability of the DMFC should be achieved. Understanding the long-term behavior of membrane-electrode assembly (MEA) is a prerequisite to this purpose and the optimization of the MEA is also needed. In this study we have investigated the changes in performance and electrochemical properties of the MEA during extended operation and the effects of heat treatment of MEA on the long-term performance. The MEAs have been treated in an autoclave with saturated water vapor at 120$^{\circ}C$, vacuum oven at 140$^{\circ}C$ and boiling in organic solvents. The autoclaved MEA was found to be have the best long term performance. The on-off operation mode also increased the performance probably due to effective removal of products from the electrodes. Physical and electrochemical analyses using a scanning electron microscope, impedance analyser and half-cell technique have been done to characterize the MEAs.

• Journal of Nutrition and Health
• /
• 제37권3호
• /
• pp.182-192
• /
• 2004

Conjugated linoleic acid (CLA) is the mixture of positional and geometric isomers of linoleic acid (LA), which is found abundantly in dairy products and meats. This study was performed to investigate the anticarcinogenic effect of CLA in HepG2 hepatoma cells. HepG2 cell were treated with LA and CLA at the various concentrations of 10, 20, 40, 80 uM each at different incubation times. After each incubation times, cell proliferation, fatty acids incorporation into cell, peroxidation and postaglandin E$_2$ (PGE$_2$) and thromboxane $A_2$ (TXA$_2$) for the eicosanoid metabolism were measured. LA treated HepG2 cells were increased cell growth 6 - 70％ of control whereas CLA increased cell death the half of those in LA group (p 〈 0.001). LA and CLA were incorporated very well into the cellular membranes four times higher than in control according to concentration and longer incubation times. Moreover, LA synthesized significantly arachidonic acids corresponding with LA concentration compared to CLA supplementation. The supplementation with LA increased intracellular lipid peroxides concentration corresponding with LA concentration and five times higher than those in CLA significantly at any incubation times (p 〈 0.001). PGE$_2$ and TXA$_2$ levels were three to twenty times lower in condition of CLA treatments than LA, respectively. Overall, the dietary CLA might change the HepG2 cell growth by the changes of cell composition, production of lipid peroxide. Since CLA have not changed the levels of arachidonic acid of cell membrane, which was sources of eicosanoids, eicosanoid synthesis was not increased in CLA compared to LA. Our results was suggest CLA has a possibility to protect the progress of atherosclerosis because CLA does not produce lipid production and endothelial contraction factors in liver.

• 전자공학회논문지SC
• /
• 제46권2호
• /
• pp.72-77
• /
• 2009

본 논문은 별도 기준 클록 없이 고속 시리얼 데이터 통신을 위한 3.2Gb/s 클록 데이터 복원(CDR) 회로를 설명한다. CDR회로는 전체적으로 5부분으로 구성되며, 위상검출기(PD)와 주파수 검출기(FD), 다중 위상 전압 제어 발진기(VCO), 전하펌프(CP), 외부 루프필터(LF)로 구성되어 있다. CDR회로는 half-rate bang-bang 타입의 위상 검출기와 입력 pull-in 범위를 늘릴 수 있도록 half-rate 주파수 검출기를 적용하였다. VCO는 4단의 차동 지연단(delay cell)으로 구성되어 있으며 튜닝 범위와 선형성 향상을 위해 rail-to-rail 전류 바이어스단을 적용하였다 각 지연단은 풀 스윙과 듀티의 부정합을 보상할 수 있는 출력 버퍼를 갖고 있다. 구현한 CDR회로는 별도의 기준 클록 없이 넓은 pull-in 범위를 확보할 수 있으며 기준 클록 생성을 위한 부가적인 Phase-Locked Loop를 필요치 않기 때문에 칩의 면적과 전력소비를 효과적으로 줄일 수 있다. 본 CDR 회로는 0.18um 1P6M CMOS 공정을 이용하여 제작하였고 루프 필터를 제외한 전체 칩 면적은 $1{\times}1mm^2$이다. 3.2Gb/s 입력 데이터 율에서 모의실험을 통한 복원된 클록의 pk-pk 지터는 26ps이며 1.8V 전원전압에서 전체 전력소모는 63mW로 나타났다. 동일한 입력 데이터 율에서 테스트를 통한 pk-pk 지터 결과는 55ps였으며 신뢰할 수 있는 입력 데이터율 범위는 약 2.4Gb/s에서 3.4Gb/s로 나타났다.