• Journal of the Korean housing association
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• v.18 no.1
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• pp.61-72
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• 2007

The purposes of this study were to summarize the concept of well-being and well-being apartment, to grasp the present condition of apartments which were introduced with well-being elements, and to find out the consumer preferences on well-being elements for apartment planning. Library and internet surveys were performed to summarize the concept of well-being and well-being apartment and to grasp the present condition of apartments which were introduced with well-being elements. Questionnaire survey was carried out from 2nd to 22nd of June 2005, to investigate the preferences on well-being elements for apartment planning. The respondents were 250 residents who are from thirties to fifties and living in urban area. As results, respondents think that 'living for health of body and mind' about concept of well-being and 'certificated apartments by green building rating system' or 'apartments introduced ecological factor' about concept of well-being apartment. They answered that 'yes' about 'Do you have intention to buy well-being apartment?'. The elements in aspect of complex planning having the preference were revealed that promenade for complex design, ecological garden or walking space for landscape design, outdoor exercise space for outdoor design, and security system for foundation equipment. The elements having the preference in aspect of public facilities were fitness room for sports & health facility and study room for cultural facility. The preferred elements in aspect of building and unit design were roof garden for building design, multi-functional room for unit floor plan, natural surface material for interior surface, ventilation system for indoor environment, control system for home automation, and food waste machine for home electronics.

• Research in Plant Disease
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• v.17 no.2
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• pp.111-120
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• 2011

A bacterial artificial chromosome library of Pectobacterium carotovorum 35 was constructed to characterize the genome and to sequence its hrp region. The hrp cluster of P. carotovorum 35 consisted of 26 open reading frames in five operons. A promoter-based green fluorescent protein technology was used to identify the genes regulated by the alternative sigma factor, HrpL, in P. carotovorum 35. The majority of the selected clones contained the hrpJ operon promoter sequence, which harbors a hrp box, but no putative hrp boxes were detected within the promoter sequences of two other hrpL-regulated genes encoding for pectate lyase and large repetitive protein. Although the promoters of five other hrp operons also contained hrp boxes, their expression was not HrpL-dependent in the promoter-based selection in E. coli. However, transcriptional analysis showed that expression from all operons harboring hrp boxes, except for the hrpN operon, was reduced significantly in the hrpL mutant. The severity of soft-rot symptoms when the hrpL mutant was applied to the surface of tobacco leaves, mimicking natural infection, was greatly attenuated. These results indicate that the hrpL gene of P. carotovorum 35 may be involved in the development of soft-rot symptoms.

• The Korean Journal of Food And Nutrition
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• v.32 no.6
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• pp.717-729
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• 2019

The chemical informs about 70 individual phenolic compounds were constructed from various lettuce samples based on literature sources and analytical data. A total of 30 phenolic compounds including quercetin 3-O-glucuronide, quercetin 3-O-(6''-O- malonyl) glucoside, cyanidin 3-O-(6''-O-malonyl)glucoside, chlorogenic acid and chicoric acid as major components were identified in 6 lettuce samples from Korea using UPLC-DAD-QToF/MS on the basis of constructed library. Among these, quercetin 3,7-di-O-glucoside(m/z 627 [M+H]⁺), quercetin 3-O-(2''-O-malonyl)glucoside(morkotin C, m/z 551 [M+H]⁺), quercetin 3-O-(6''- O-malonyl)glucoside methyl ester(m/z 565 [M+H]⁺), 5-O-cis-p-coumaroylquinic acid(m/z 339 [M+H]⁺) and 5-O-caffeoylquinic acid methyl ester(m/z 369 [M+H]⁺) were newly confirmed from the lettuce samples. In total content of phenolic compounds, 4 red lettuce samples(2,947.7~7,535.6 mg/100 g, dry weight) showed higher than green lettuce(2,687.3 mg) and head lettuce(320.1 mg).

• Journal of Cadastre & Land InformatiX
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• v.44 no.2
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• pp.103-112
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• 2014

The objective of this study is to suggest the possibility on land cover classification using hyperspectal imagery on area which includes lands and waters. After atmospheric correction as a preprocessing work was conducted on hyperspectral imagery acquired by airborne hyperspectral sensor CASI-1500, the effect of atmospheric correction to a few land cover class in before and after atmospheric correction was compared and analyzed. As the result of accuracy of land cover classification by highspectral imagery using reference data as airphoto and digital topographic map, maximum likelihood method represented overall accuracy as 67.0% and minimum distance method showed overall accuracy as 52.4%. Also product accuracy of land cover classification on road, dry field and green house, but that on river, forest, grassland showed low because the area of those was composed of complex object. Therefore, the study needs to select optimal band to classify specific object and to construct spectral library considering spectral characteristics of specific object.

• Journal of Microbiology and Biotechnology
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• v.30 no.9
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• pp.1430-1435
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• 2020

Bacterial cellulose (BC) has outstanding physical and chemical properties, including high crystallinity, moisture retention, and tensile strength. Currently, the major producer of BC is Komagataeibacter xylinus. However, due to limited tools of expression, this host is difficult to engineer metabolically to improve BC productivity. In this study, a regulated expression system for K. xylinus with synthetic ribosome binding site (RBS) was developed and used to engineer a BC biosynthesis pathway. A synthetic RBS library was constructed using green fluorescent protein (GFP) as a reporter, and three synthetic RBSs (R4, R15, and R6) with different strengths were successfully isolated by fluorescence-activated cell sorting (FACS). Using synthetic RBS, we optimized the expression of three homologous genes responsible for BC production, pgm, galU, and ndp, and thereby greatly increased it under both static and shaking culture conditions. The final titer of BC under static and shaking conditions was 5.28 and 3.67 g/l, respectively. Our findings demonstrate that reinforced metabolic flux towards BC through quantitative gene expression represents a practical strategy for the improvement of BC productivity.

• Korean Journal of Food Science and Technology
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• v.32 no.5
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• pp.997-1001
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• 2000

The organic acids in 3 kinds of medicinal herbal extracts were screened and compared each other according to their organic acid contents by an efficient gas chromatographic method. It involves solid-phase extraction of organic acids using Chromosorb P with subsequent conversion to stable tert-butyldimethysilyl derivatives for the direct analysis by capillary column gas chromatography and gas chromatography-mass spectrometry. Total of 24 organic acids were reproducibly identified from 3 kinds of herbal extracts. When the GC profiles were simplified to their retention index spectra, characteristic patterns were obtained for each herb sample. As expected, three kinds of herbal extracts showed three distinct patterns.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.65.2-65
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• 2003

To protect the plant against several soil-borne pathogens, we are currently constructing disease-resistant transgenic root stock for the growth of cucurbitaceae vegetable plants, watermelon and gourd. We made a watermelon cDNA library from Cladosporium cucumerinum-Infected leaves for substractive hybriazation and differential screening. We isolated the several pathogen inducible cDNA clones, such as caffeoyl-CoA-methyltransferase, LAA induced protein, receptor-like kinase homolog, hydroxyproline-rich glycoprotein, catalase, calmodulin binding protein, mitochondrial ATPase beta subunit, methyl tRNA synthetase and WRKY transcription factors. We previously obtained CaMADS in pepper and galactinol synthase ( CsGolS) in cucumber that were confirmed to be related with disease-resistance. CaMADS and CsGolS2 were transformed into the inbred line 'GO701-2' gourd, the inbred line '6-2-2' watermelon and the Kong-dye watermelon by Agrobacterium tumerfaciens LBA4404. Plant growth regulators (zeatin, BAP and IAA) were used for shoot regeneration and root induction for optimal condition. Putative transgenic plants were selected in medium containing 100mg/L kanamycin and integration of the CaMADS and CsGO/S2 into the genomic DNA were demonstrated by the PCR analysis. We isolated major soil-borne pathogens, such as Monosporascus cannonballus, Didymella bryoniae, Cladosporium cuvumerinum from the cultivation area of watermelon or root stock, and successfully established artificial inoculation method for each pathogen. This work was supported by a grant from BioGreen 21 program, Rural Development Administration, Republic of Korea.

• Journal of KIISE:Software and Applications
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• v.30 no.7_8
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• pp.727-743
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• 2003

This paper proposes an automatic highlight building algorithm for soccer video by using the structural characteristics of broadcasted sports video that an interesting (or important) event (such as goal or foul) in sports video has a continuous replay shot surrounded by gradual shot change effect like wipe. This shot editing rule is used in this paper to analyze the structure of broadcated soccer video and extracts shot involving the important events to build a highlight. It first uses the spatial-temporal image of video to detect wipe transition effects and zoom out/in shot changes. They are used to detect the replay shot. However, using spatial-temporal image alone to detect the wipe transition effect requires too much computational resources and need to change algorithm if the wipe pattern is changed. For solving these problems, a two-pass detection algorithm and a pixel sub-sampling technique are proposed in this paper. Furthermore, to detect the zoom out/in shot change and replay shots more precisely, the green-area-ratio and the motion energy are also computed in the proposed scheme. Finally, highlight shots composed of event and player shot are extracted by using these pre-detected replay shot and zoom out/in shot change point. Proposed algorithm will be useful for web services or broadcasting services requiring abstracted soccer video.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.43 no.11 s.353
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• pp.26-36
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• 2006

In this paper, we propose a cost-effective color filter may (CFA) demosaicing method for digital still cameras in which a single CCD or CMOS image sensor is used. Since a CFA is adopted, we must interpolate missing color values in the red, green and blue channels at each pixel location. While most state-of-the-art algorithms invest a great deal of computational effort in the enhancement of the reconstructed image to overcome the color artifacts, we focus on eliminating the color artifacts with low computational complexity. Using spatial correlation of the adjacent pixels, the edge-directional information of the neighbor pixels is used for determining the edge direction of the current pixel. We apply our method to the state-of-the-art algorithms which use edge-directed methods to interpolate the missing color channels. The experiment results show that the proposed method enhances the demosaiced image qualify from $0.09{\sim}0.47dB$ in PSNR depending on the basis algorithm by removing most of the color artifacts. The proposed method was implemented and verified successfully using verilog HDL and FPGA. It was synthesized to gate-level circuits using 0.25um CMOS standard cell library. The total logic gate count is 12K, and five line memories are used.

• Journal of Plant Biotechnology
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• v.41 no.3
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• pp.159-167
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• 2014

In eukaryotes, proteins that are secreted into ER are post-translationally modified by N-glycosylation, the patterns of which are significantly different between plant and animal cells. Biotechnology industry has already produced a number of therapeutic glycoproteins in plant cells. However, the aberrant glycosylation of therapeutic recombinant proteins in plant systems can cause immune problems in humans. Therefore, it is important to develop strategies for producing non-glycosylated forms to preserve biological activity and native conformation by a peptide: N-glycanase (PNGase). In this study, we try to isolate PNGase T gene from tomato, which can use as a platform plant for biotechnology industry. We isolated a cDNA (GenBank Accession number KM401550) from tomato leaves with 1,767 bp, which encoded a polypeptide of 588 amino acids with a predicted molecular mass of 65.8 kDa. We also investigated the expression patterns of PNGase T during fruit ripening of tomato. The transcripts of PNGase T, which were constitutively induced in tomato fruit from green stage, were significantly increased and reached a peak at orange stage. After which, those transcripts were continuously reduced. The expression pattern of PNGase T was coincided well with transcripts profiles of metacaspase gene, LeMCA, and senescence-related gene members of ACC synthase, LeACS2, LeACS4, and LeACS6, for ethylene biosynthesis during fruit ripening. These results suggest that PNGase T is involved in a de-glycosylation process associated with senescence and fruit ripening.