• 한국식생활문화학회지
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• 제36권3호
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• pp.325-332
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• 2021

This study sought to investigate the effects of heat-moisture treatment (HMT) and ethanol treatment (EOH) for improving the quality and storage stability of Tteokbokki Tteok. The quality characteristics were evaluated by moisture, pH, color, texture profile analysis, and observing the microbial properties after the heat-moisture treatment or ethanol treatment. As the storage period increased, the moisture content of Tteokbokki Tteok tended to decrease except for the HMT group (p<0.05) while the pH did not show significant variation except for the EOH group (p<0.05). While measuring the color, the L-value tended to increase in all groups during the storage period. The a-value and the b-value showed the highest values in the HMT group and the control (CON) group, respectively. In the texture profile analysis, all groups showed a significant tendency to increase levels of hardness and chewiness as the storage period increased (p<0.05). The HMT group showed an increase in hardness and adhesiveness, which are characteristics of the HMT treatment. The results of examining the microbial properties of Tteokbokki Tteok showed that the total microbial count in the HMT group was 4.52 on the 8^th day of storage, which was lower than the level in the CON group and the EOH group on the 4^th day of storage. Yeast and mold were not measured during the storage period. Thus the results of this study showed that when manufacturing Tteokbokki Tteok, the heat-moisture treatment of rice powder increased the storage stability by delaying microbial growth and also had positive effects on quality.

• 국제지역연구
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• 제22권1호
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• pp.101-121
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• 2018

본 논문은 중국내 다국적 기업 R&D 랩의 진출동기가 경영성과에 미치는 영향, 진출동기와 경영성과의 관계에서 통제수준의 매개효과에 대하여 검증하였다. 문헌연구에 대한 고찰을 거쳐, 다국적 기업 R&D 랩의 진출 동기는 크게 4가지로 드러났다. 첫째, 중국의 시장과 고객을 확보하기 위한 목적으로 시장요인이라 명명하였으며, 둘째, 중국의 훌륭한 기술이나 인적자원을 획득하기 위한 목적으로 자원요인이라 명명하였다. 셋째, 정부의 혜택, 관련 정책, 인센티브 조항 등 제도적 이점을 바라고 진출하는 경우인데 이를 제도요인이라 명명하였고 마지막으로 중국을 전략적 요지로 보고 중국 시장에서 경쟁자와 경쟁하기 위한 목적으로 진출한 경우인데 이를 전략요인이라 명명하였다. R&D 랩에 대한 기업의 통제수준은 각 사안별로 측정하였다. 진출동기와 통제수준의 관계를 실증분석해본 결과, 시장요인, 제도요인, 전략요인을 추구하는 기업들은 목표의 특성상 현지성을 많이 추구해야 하므로 높은 통제보다는 자율권을 부여하는 것이 바람직 한 것으로 드러났다. 진출동기와 경영성과의 관계에서 통제수준의 매개효과를 살펴본 결과, 자원요인을 추구하여 진출한 R&D 랩은 현지에서 획득한 훌륭한 기술자원을 잘 활용하기 위하여 본사의 통제가 필요로 하며, 전략요인을 추구하는 기업 역시 본사의 통제력이 강할수록 경영성과가 높아 경쟁에서 유리한 것으로 나타났다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.73-73
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• 2019

Angelica gigas Nakai (Korean danggui), a member of the Umbelliferae family, is a Korean traditional medicinal plant whose roots have been used for treating gynecological diseases. Transcriptomics is the study of the transcriptome, which is the complete set of RNA transcripts that are produced by the genome, using high-throughput methods, such as microarray analysis. In this study, transcriptome analysis of A.gigas Nakai was carried out. Transcriptome sequencing and assembly was carried out by using Illumina Hiseq 2500, Velvet and Oases. A total of 109,591,555 clean reads of A. gigas Nakai was obtained after trimming adaptors. The obtained reads were assembled with an average length of 1,154 bp, a maximum length of 13,166 bp, a minimum length of 200 pb, and N50 of 1,635 bp. Functional annotation and classification was performed using NCBI NR, InterprotScan, KOG, KEGG and GO. Candidate genes for phenylpropanoid biosynthesis were obtanied from A.gigas transcriptome and the genes and its proteins were confirmed through the NCBI homology BLAST searches, revealing high identity with other othologous genes and proteins from various plants pecies. In RNA sequencing analysis using an Illumina Next-Seq2500 sequencer, we identified a total 94,930 transcripts and annotated 71,281 transcripts, which provide basic information for further research in A.gigas Nakai. Our transcriptome data reveal that several differentially expressed genes related to flower color in A.gigas Nakai. The results of this research provide comprehensive information on the A.gigas Nakai genome and enhance our understanding of the flower color related gene pathways in this plant.

• 한국약용작물학회지
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• 제28권6호
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• pp.395-411
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• 2020

Background: This study investigated the anti-obesity effect of the flavonoid rich fraction (FRF) and its constituent, rutin obtained from the leaf of Morus alba L., on the lipid accumulation mechanism in 3T3-L1 adipocyte and C57BL/6 mouse models. Methods and Results: In Oil Red O staining, FRF (1,000 ㎍/㎖) treatments showed inhibition rate of 35.39% in lipid accumulation compared to that in the control. AdipoRedTM assay indicated that the triglyceride content in 3T3-L1 adipocytes treated with FRF (1,000 ㎍/㎖) was reduced to 23.22%, and free glycerol content was increased to 106.04% that of the control. FRF and its major constituent, rutin affected mRNA gene expression. Rutin contributed to the inhibition of Sterol regulatory element binding protein-1c (SREBP-1c) gene expression, and inhibited the transcription factors SREBP-1c, peroxisome proliferator-activated receptor gamma (PPAR-γ), CCAAT/enhancer binding protein α (C/EBPα), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC). In addition, the effect of FRF administration on obesity development in C57BL/6 mice fed high-fat diet (HFD) was investigated. FRF suppressed weight gain, and reduced liver triglyceride and leptin secretion. FRF exerted potential anti-inflammatory effects by improving insulin resistance and adiponectin levels, and could thus be used to help counteract obesity. The mRNA expressions of PPAR-γ, FAS, ACC, and CPT-1 were determined in liver tissue. Quantitative real-time PCR analysis was also performed to evaluate the expression of IL-1β, IL-6, and TNF-α in epididymal adipose tissue. Compared to the control group, mice fed the HFD showed the up-regulation in PPAR-γ, FAS, IL-6, and TNF-α genes, and down-regulation in CPT1 gene expression. FRF treatement markedly reduced the expression of PPAR-γ, FAS, IL-6, and TNF-α compared to those in HFD control, whereas increased the expression level of CPT1. Conclusions: These results suggest that the FRF and its major active constituent, rutin, can be used as effective anti-obesity agents.

• 한국식품위생안전성학회지
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• 제35권6호
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• pp.567-573
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• 2020

감은 중국, 한국, 일본, 브라질, 터키, 이탈리아 등을 포함하는 온대지역에서 널리 재배되고 있으며 일부 아시아권 소비자들에게는 건강에 유익한 기능성 원료로 인식되고 있다. 또한 감에 포함된 풍부한 파이토케미컬들은 감을 섭취함으로써 건강과 관련된 다양한 문제점을 개선하기 위한 연구의 가능성을 제시한다. 본 연구에서는 감의 미숙과인 풋감추출물(DKA)의 유전독성을 확인하고자 한다. 미생물복귀돌연변이시험, 염색체이상시험, 포유류 소핵발생시험을 수행하여 풋감추출물(DKA)의 유전독성을 평가하였다. 미생물복귀돌연변이시험에서 DKA는 Salmonella typhimurium TA98, TA100, TA1535, TA1537 와 Escherichia coli WP2uvrA에서 S9 대사활성계의 존재에 상관없이 돌연변이 유도를 보이지 않았다. 또한 마우스를 이용한 소핵시험은 풋감추출물(DKA)처리군에서 소핵을 가진 다염성 적혈구와 전체적혈구 중 다염성 적혈구의 비율의 증가는 볼 수 없었으며 통계학적 유의성도 나타나지 않았다. 한편, CHL 세포를 이용한 염색체이상시험에서 모든 세포주의 처리시간 및 S9 대사활성계 존재유무에 상관없이 염색체이상을 보이지 않았다. 따라서 본 연구결과에 의하면 풋감추출물(DKA)은 유전독성을 유발하지 않는 안전한 기능성 식품 원료로서 활용 가능하다고 판단된다.

• 한국환경보건학회지
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• 제47권6호
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• pp.548-557
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• 2021

Background: Due to the emergence of new diseases such as COVID-19, an increasing number of people are struggling with stress and depression. Interest is growing in forest-based recreation for physical and mental relief. Objectives: A prediction model equation using meteorological factors and data was developed to predict the quantities of medicinal substances generated in forests (monoterpenes) in real-time. Methods: The concentration of phytoncide and meteorological factors in the forests near Chuncheon in South Korea were measured for nearly two years. Meteorological factors affecting the observation data were acquired through a multiple regression analysis. A model equation was developed by applying a linear regression equation with the main factors. Results: The linear regression analysis revealed a high explanatory power for the coefficients of determination of temperature and humidity in the coniferous forest (R²=0.7028 and R²=0.5859). With a temperature increase of 1℃, the phytoncide concentration increased by 31.7 ng/Sm³. A humidity increase of 1% led to an increase in the coniferous forest by 21.9 ng/Sm³. In the deciduous forest, the coefficients of determination of temperature and humidity had approximately 60% explanatory power (R²=0.6611 and R²=0.5893). A temperature increase of 1℃ led to an increase of approximately 9.6 ng/Sm³, and 1% humidity resulted in a change of approximately 6.9 ng/Sm³. A prediction model equation was suggested based on such meteorological factors and related equations that showed a 30% error with statistical verification. Conclusions: Follow-up research is required to reduce the prediction error. In addition, phytoncide data for each region can be acquired by applying actual regional phytoncide data and the prediction technique proposed in this study.

• Journal of Microbiology and Biotechnology
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• 제32권9호
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• pp.1154-1167
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• 2022

In this study, we investigated the anti-amnesic effect of Korean red pine (Pinus densiflora) bark extract (KRPBE) against amyloid beta_1-42 (Aβ_1-42)-induced neurotoxicity. We found that treatment with KRPBE improved the behavioral function in Aβ-induced mice, and also boosted the antioxidant system in mice by decreasing malondialdehyde (MDA) content, increasing superoxide dismutase (SOD) activities, and reducing glutathione (GSH) levels. In addition, KRPBE improved the cholinergic system by suppressing reduced acetylcholine (ACh) content while also activating acetylcholinesterase (AChE), regulating the expression of choline acetyltransferase (ChAT), postsynaptic density protein-95 (PSD-95), and synaptophysin. KRPBE also showed an ameliorating effect on cerebral mitochondrial deficit by regulating reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and ATP levels. Moreover, KRPBE modulated the expression levels of neurotoxicity indicators Aβ and phosphorylated tau (p-tau) and inflammatory cytokines TNF-α, p-IκB-α, and IL-1β. Furthermore, we found that KRPBE improved the expression levels of neuronal apoptosis-related markers BAX and BCl-2 and increased the expression levels of BDNF and p-CREB. Therefore, this study suggests that KRPBE treatment has an anti-amnestic effect by modulating cholinergic system dysfunction and neuroinflammation in Aβ_1-42-induced cognitive impairment in mice.

• Journal of Animal Science and Technology
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• 제64권1호
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• pp.23-37
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• 2022

Thirty-six weaned piglets with an initial body weight (BW) of 8.43 ± 0.40 kg (28 days of age, ([Landrace × Yorkshire] × Duroc) were randomly assigned to 6 treatments for a 2-week feeding trial to determine the effects of different inorganic zinc (IZ), organic zinc (OZ) or combination of low crude protein diet (LP) and Mixed feed additive (MFA) on diarrhea score, nutrient digestibility, zinc utilization, blood profiles, organ weight, and fecal microflora in weaned piglet diet. The pigs were individually placed in 45 × 55 × 45 cm stainless steel metabolism cages in an environmentally controlled room (30 ± 1℃). The dietary treatments included a negative control (NC), positive control (PC; zinc oxide, 1,000 mg/kg), T1 (IZ : OZ, 850 : 150), T2 (IZ : OZ 700 : 300), T3 (IZ : OZ, 500 : 500), and T4 (LP + MFA [0.1% Essential oils + 0.08% Protease + 0.02% Xylanase]). The daily feed allowance was adjusted to 2.7 times the maintenance requirement for digestible energy (2.7 × 110 kcal of DE/kg BW^0.75). This allowance was divided into two equal parts, and the piglets were fed at 08 : 30 and 17 : 30 each day. Water was provided ad libitum through a drinking nipple. The diarrhea score was significantly increased (p < 0.05) in NC treatment compared with other treatments. The apparent total tract digestibility (ATTD) of dry matter (DM), nitrogen (N), and gross energy (GE) was significantly increased (p < 0.05) in the T2 treatment compared with the PC and NC treatments in week 1. In week 2, the ATTD of DM, N, and GE was significantly decreased (p < 0.05) in the NC treatment compared with other treatments. The T3 treatment had significantly higher (p < 0.05) ATTD and apparent ileal digestibility of zinc than the PC and T1 treatments. The Escherichia coli count in feces was significantly decreased in the T4 treatment compared with the NC and T2 treatments. The Lactobacillus count in feces was significantly increased in the T4 and T1 treatment compared with the T2 and T3 treatments. In conclusion, IZ : OZ 500 : 500 levels could improve nutrient digestibility and zinc utilization in weaned piglets, Moreover, MFA in LP diets could be used as a zinc alternative.

• 한국노년학
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• 제29권3호
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• pp.899-915
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• 2009

본 연구의 목적은 체력과 운동참여가 노인들의 건강한 노후에 미치는 영향에 대해 조사한 것으로 연구대상은 서울시 복지관에 다니고 운동에 참여하는 65세 이상의 노인 350명(남 96명, 여 223명)을 대상으로 하였고, 총 319부의 설문지와 체력측정을 실제 분석 자료로 사용하였다. 조사기간은 2006년 11월 1부터 11월 30일까지 설문조사와 체력측정을 실시하였으며, 자료처리는 Window용 SPSS 통계 Package 12.0 Version을 이용하여 빈도분석, 기술통계, 상관관계, 위계적 회귀분석 방법을 실시하였다. 위계적 회귀분석 결과는 다음과 같이 나타났다. 첫째, 월수입, 한달용돈, 주관적 건강상태, 하체근력, 운동행태, 참여기간이 건강한 노후에 영향을 주는 요인으로 나타났다. 둘째, 건강한 노후의 하위요인인 신체적 건강에서는 나이, 결혼유지 상태, 주관적 건강, 운동 행태, 운동참여기간이 영향을 미치는 요인으로 나타났다. 셋째, 건강한 노후의 하위요인인 인지·정신적 건강에서는 월수입, 한달용돈, 주관적 건강상태, 하체근력, 운동행태, 운동참여기간이 영향을 미치는 요인으로 나타났다. 넷째, 건강한 노후의 하위요인인 사회적·지지 건강에서는 월수입, 나이, 학력, 운동행태, 운동참여기간이 영향을 미치는 요인으로 나타났다. 따라서 이상과 같은 결과를 통해 얻어낸 결론은 다음과 같다. 첫째, 본 연구의 결과를 통해 한국 노인들의 건강한 노후를 반영했다는 점에서 의의가 크다. 둘째, 통계적용을 통해 얻어낸 결과 중에 운동행태에 대한 요인이 건강한 노후를 유지하는데 중요한 요소라는 점을 부각시켜야 할 것이다. 셋째, 노인들의 건강한 노후의 문항 점수에 관한 기준값 제시에 의의가 있다.

• 서비스연구
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• 제13권4호
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• pp.24-46
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• 2023

본 연구는 서비스 조직의 내부시장 지향성이 서비스 제공자의 적응적 서비스 행동에 어떠한 영향을 미치는지 살펴보고, 이를 위하여 어떠한 심리적 과정을 거치는지 확인하고자 하였다. 이러한 목적을 달성하기 위하여 선행연구를 바탕으로 서비스 조직의 내부시장 지향성이 서비스 제공자의 인지적 측면인 개인-환경 적합성과 정서적 측면인 직무열의의 심리적 과정을 연속으로 매개하여 적응적 서비스 행동을 증가시킬 것으로 가정하고, 가설 및 연구모델을 설정하였다. 가설검증을 위하여 부산과 경남지역에 위치하는 위탁급식업체의 영양사를 대상으로 설문조사를 진행하고 데이터를 수집하였다. 본 연구의 연구모델에서 제안된 가설을 검증한 결과 내부시장 지향성은 개인-환경 적합성의 핵심요소인 개인-직업 적합성과 개인-조직 적합성을 증가시켰으며, 개인-직업 적합성과 개인-조직 적합성은 직무열의에 긍정적인 영향을 미치는 것을 확인하였다. 또한, 직무열의는 서비스 제공자의 적응적 서비스 행동을 향상시키는 것을 확인하였다. 마지막으로, 적응적 서비스 행동에 대한 내부시장 지향성의 영향력은 개인-환경 적합성과 직무열의를 연속적으로 매개함을 본 연구에서 확인하였다.