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http://dx.doi.org/10.13103/JFHS.2020.35.6.567

Genotoxicity Study of Immature Green Persimmon Extract  

Ham, Young-Min (Biodiversity Research Institute, Jeju Technopark)
Yoon, Seon-A (Biodiversity Research Institute, Jeju Technopark)
Hyun, Ho Bong (Biodiversity Research Institute, Jeju Technopark)
Go, Boram (Biodiversity Research Institute, Jeju Technopark)
Jung, Yong-Hwan (Biodiversity Research Institute, Jeju Technopark)
Oh, Dae-Ju (Biodiversity Research Institute, Jeju Technopark)
Yoon, Weon-Jong (Biodiversity Research Institute, Jeju Technopark)
Publication Information
Journal of Food Hygiene and Safety / v.35, no.6, 2020 , pp. 567-573 More about this Journal
Abstract
The persimmon is commonly cultivated in temperate regions of the world, including China, Korea, Japan, Brazil, Turkey, and Italy. In some Asian cultures, consumers are aware of the health claims related to the persimmon and its functional ingredients. The rich phytochemistry of the persimmon has opened new avenues of research on diet-based regimens designed to cure various ailments. This study was conducted to identify the genotoxicity of immature green persimmon (Diospyros kaki THUNB.) extract (DKA). The bacterial reverse mutation assay, the chromosomal aberration assay, and the mammalian micronucleus test were performed to determine the DKA genotoxicity. The result of the bacterial reverse mutation assay revealed that the DKA did not induce mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, TA1537 and Escherichia coli WP2uvrA with or without metabolic activation of S9 mixture. The oral administration of DKA also caused no significant increase in the number of micronucleated polychromatic erythrocytes or in the mean ratio of polychromatic to total erythrocytes. In addition, DKA did not cause a significant chromosome aberration on CHL cells in the presence or absence of S9 activation. In conclusion, DKA could be considered as a reliable and safe functional food since no toxicity was found under the condition of this study.
Keywords
Immature persimmon; Genotoxicity; Reverse mutation; Micronucleus formation; Chromosome aberration;
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